Dome growth and explosive degassing are fundamental processes in the cycle of continental arc volcanism. Johnson et al. present novel optical geodetic observations to constrain the sources responsible for long-period volcanic earthquakes, which are coincident with frequent explosive eruptions at Santiaguito volcano, Guatemala. They find that acceleration in deformation of the volcanic dome, extracted from high-resolution optical image processing, is coincident with recorded long-period seismic sources. Based on these observations, abrupt mass shift of solidified domes, conduit magma or magma pads seem to be part of the mechanism responsible for generating long-period earthquakes at silicic volcanic systems. This study presents optical geodetic observations to constrain the sources responsible for long-period volcanic earthquakes, which are coincident with frequent explosive eruptions at Santiaguito Volcano, Guatemala. It is found that acceleration in deformation of the volcanic dome, extracted from the high-resolution optical image processing, is coincident with recorded long-period seismic sources. On the basis of these observations, abrupt mass shift of solidified domes, conduit magma or magma pads seem to be part of the mechanism responsible for generating long-period earthquakes at silicic volcanic systems. Dome growth and explosive degassing are fundamental processes in the cycle of continental arc volcanism1,2. Because both processes generate seismic energy, geophysical field studies of volcanic processes are often grounded in the interpretation of volcanic earthquakes3. Although previous seismic studies have provided important constraints on volcano dynamics4,5,6, such inversion results do not uniquely constrain magma source dimension and material properties. Here we report combined optical geodetic and seismic observations that robustly constrain the sources of long-period volcanic earthquakes coincident with frequent explosive eruptions at the volcano Santiaguito, in Guatemala. The acceleration of dome deformation, extracted from high-resolution optical image processing, is shown to be associated with recorded long-period seismic sources and the frequency content of seismic signals measured across a broadband network. These earthquake sources are observed as abrupt subvertical surface displacements of the dome, in which 20–50-cm uplift originates at the central vent and propagates at ∼50 m s-1 towards the 200-m-diameter periphery. Episodic shifts of the 20–80-m thick dome induce peak forces greater than 109 N and reflect surface manifestations of the volcanic long-period earthquakes, a broad class of volcano seismic activity that is poorly understood and observed at many volcanic centres worldwide7. On the basis of these observations, the abrupt mass shift of solidified domes, conduit magma or magma pads may play a part in generating long-period earthquakes at silicic volcanic systems.