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Your search keyword '"Spiridonova, K. V."' showing total 3 results
Start Over Author "Spiridonova, K. V." Topic gentiana
3 results on '"Spiridonova, K. V."'

1. [18S-25S rDNA variation in tissue culture of some Gentiana L. species].

Author

Mel'nyk VM, Andrieiev IO, Spiridonova KV, Strashniuk NM, and Kunakh VA

Subjects
Electrophoresis, Agar Gel, In Situ Hybridization, Tissue Culture Techniques, DNA, Plant genetics, DNA, Ribosomal genetics, Gentiana genetics, Polymorphism, Restriction Fragment Length, RNA, Ribosomal genetics, RNA, Ribosomal, 18S genetics
Abstract

18S-25S rDNA of intact plants and tissue cultures of G. acaulis, G. punctata and G. lutea have been investigated by using blot-hybridization. The decrease of rDNA amount was found in the callus cultures as compared with the plants. In contrast to other species, G. lutea showed intragenome heterogeneity of rRNA genes as well as qualitative rDNA changes in tissue culture, in particular appearance of altered repeats. The relationship between the peculiarities of rRNA gene structure and their rearrangements in in vitro culture was suggested.

Published
2007

2. [Variability of nuclear 18S-25S rDNA of Gentiana lutea L. in nature and in tissue culture in vitro].

Author

Mel'nyk VM, Spiridonova KV, Andrieiev IO, Strashniuk NM, and Kunakh VA

Subjects
RNA, Plant genetics, RNA, Ribosomal genetics, RNA, Ribosomal, 18S genetics, Repetitive Sequences, Nucleic Acid genetics, DNA, Plant genetics, DNA, Ribosomal genetics, Genes, Plant genetics, Genetic Variation genetics, Gentiana genetics
Abstract

18S-25S rDNA sequence in genomes of G. lutea plants from different natural populations and from tissue culture has been studied with blot-hybridization method. It was shown that ribosomal repeats are represented by the variants which differ for their size and for the presence of additional HindIII restriction site. Genome of individual plant usually possesses several variants of DNA repeats. Interpopulation variability according to their quantitative ratio and to the presence of some of them has been shown. Modifications of the range of rDNA repeats not exceeding intraspecific variability were observed in callus tissues in comparison with the plants of initial population. Non-randomness of genome modifications in the course of cell adaptation to in vitro conditions makes it possible to some extent to forecast these modifications in tissue culture.

Published
2004

3. [The investigation of genomes of some species of the genus Gentiana in nature and in vitro cell culture].

Author

Mel'nyk VM, Spiridonova KV, Andrieiev IO, Strashniuk NM, and Kunakh VA

Subjects
Cells, Cultured, Gentiana cytology, Plant Leaves genetics, Plant Roots genetics, Polymorphism, Restriction Fragment Length, Restriction Mapping, Species Specificity, DNA, Plant genetics, Genome, Plant, Gentiana genetics
Abstract

The comparative study of the genomes of intact plants-representatives of some species of the genus Gentiana L. as well as cultured cells of G. lutea and G. punctata was performed using restriction analysis. Species specificity of restriction fragment patterns for studied representatives of this genus was revealed. The differences between electrophoretic patterns of digested DNA purified from rhizome and leaves of G. lutea and G. punctata were found. The changes in genomes of G. lutea and G. punctata cells cultured in vitro compared with the genomes of intact plants were detected. The data obtained evidence that some of them may be of nonrandom character.

Published
2002

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