6 results on '"Camargo, Samira Esteves Afonso"'
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2. Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation.
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Carvalho, Nayane Chagas, Guedes, Simone Alves Garcez, Albuquerque-Júnior, Ricardo Luiz Cavalcanti, de Albuquerque, Diana Santana, de Souza Araújo, Adriano Antunes, Paranhos, Luiz Renato, Camargo, Samira Esteves Afonso, and Ribeiro, Maria Amália Gonzaga
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ALOE vera , *GENETIC toxicology , *ENDODONTICS , *PHOTOBIOCHEMISTRY , *CALCIUM hydroxide - Abstract
This study aims to evaluate, in vitro , the effect of Aloe vera associated with endodontic medication, with or without laser photobiomodulation (FTL) irradiation in FP6 human pulp fibroblasts. The materials were divided into eight groups: CTR - control; CL - FTL alone; AA - Aloe vera with distilled water; AL - Aloe vera with distilled water and FTL; HA - calcium hydroxide P.A. with distilled water; HL - calcium hydroxide P.A. with distilled water and FTL; HAA - calcium hydroxide P.A. with Aloe vera and distilled water; HAL - calcium hydroxide P.A. with Aloe vera , distilled water, and FTL. The cytotoxicity was evaluated by MTT assay at 24, 48, and 72 h and the genotoxicity by micronucleus test assay. This study was performed in triplicate. Data obtained in both tests were statistically analyzed by ANOVA and Tukey's tests ( p ≤ 0.05). Group AA presented high genotoxicity and low cytotoxicity. After 24, 48, and 72 h, the group HAA significantly reduced the cell viability. Interaction with FTL showed slightly increase cell viability after 24 and 48 h in groups CL and HL ( p < 0.001), despite the high genotoxicity in group CL and low genotoxicity in group HL. Group AL showed higher cell survival rate at 72 h ( p < 0.05) and high genotoxicity ( p < 0.001). It was concluded that Aloe vera allowed higher cell viability in human pulp fibroblasts in the presence of calcium hydroxide or with FTL separately, but genotoxicity increased in these associations. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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3. Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity.
- Author
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Oliveira, Jonatas Rafael de, de Jesus Viegas, Daiane, Martins, Ana Paula Réquia, Carvalho, Cláudio Antonio Talge, Soares, Cristina Pacheco, Camargo, Samira Esteves Afonso, Jorge, Antonio Olavo Cardoso, and de Oliveira, Luciane Dias
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THYMES , *ANTI-infective agents , *CELL-mediated cytotoxicity , *GENETIC toxicology , *MACROPHAGES , *THERAPEUTICS - Abstract
Objectives This study evaluated the biological effects of the T. vulgaris L. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity. Methods Monomicrobial biofilms of Candida albicans , Staphylococcus aureus , Enterococcus faecalis , Streptococcus mutans and Pseudomonas aeruginosa and polymicrobial biofilms composed by C. albicans with each bacterium were formed for 48 h and exposed for 5 min to the plant extract. Murine macrophages (RAW 264.7), human gingival fibroblasts (FMM-1), human breast carcinoma cells (MCF-7) and cervical carcinoma cells (HeLa) were also exposed to the plant extract for 5 min and the cell viability were analyzed by MTT, neutral red (NR) and crystal violet (CV) assays. Interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) produced by RAW 264.7 was quantified by ELISA, after 24 h exposure to the plant extract, both in the absence and presence of lipopolysaccharide (LPS) from Escherichia coli . Genotoxicity of the plant extract was evaluated by micronucleus formation (MN) in 1000 cells. The results were analyzed by T-Test or ANOVA and Tukey’s Test ( P ≤ 0.05). Results All biofilms showed significant reductions in CFU/mL (colony-forming units per milliliter). Cell viability was above 50% for all cell lines. Anti-inflammatory effect on the synthesis of IL-1β and TNF-α was observed. The MN was similar or lower than the control group in all cells. Conclusions T. vulgaris L. extract was effective against all biofilms, promoted high cell viability, anti-inflammatory effect and presented no genotoxicity. [ABSTRACT FROM AUTHOR]
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- 2017
- Full Text
- View/download PDF
4. Evaluation of biocompatibility and antimicrobial action in vitro of a hydrogel scaffolds Hydromatrix associated to glycolic extract of Punica granatum (pomegranate)
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Gomes, Livia Aparecida Procopio, Universidade Estadual Paulista (Unesp), and Camargo, Samira Esteves Afonso [UNESP]
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Punica granatum ,Scaffold ,Hydrogel ,Genotoxicidade ,Células ,Citotoxicidade ,Hidrogel ,Matriz ,Cells ,Cytotoxicity ,Enterococcus faecalis ,Genotoxicity - Abstract
Submitted by LIVIA APARECIDA PROCOPIO null (livia_procopio@hotmail.com) on 2018-02-09T15:42:21Z No. of bitstreams: 1 Versão Final 02-02-18.pdf: 922541 bytes, checksum: a4127f75f51107b997c1c6b6a97a7713 (MD5) Approved for entry into archive by Silvana Alvarez null (silvana@ict.unesp.br) on 2018-02-16T17:57:34Z (GMT) No. of bitstreams: 2 gomes_lap_sjc_int.pdf: 922541 bytes, checksum: a4127f75f51107b997c1c6b6a97a7713 (MD5) gomes_lap_sjc_par.pdf: 429939 bytes, checksum: bfd42840e604070f5c6e2f4c6fcc8862 (MD5) Made available in DSpace on 2018-02-16T17:57:34Z (GMT). No. of bitstreams: 2 gomes_lap_sjc_int.pdf: 922541 bytes, checksum: a4127f75f51107b997c1c6b6a97a7713 (MD5) gomes_lap_sjc_par.pdf: 429939 bytes, checksum: bfd42840e604070f5c6e2f4c6fcc8862 (MD5) Previous issue date: 2017-12-05 A matriz de hidrogel é um biomaterial de nanofibra peptídica tridimensional que induz o crescimento, migração e proliferação celular, assim favorecendo a regeneração tecidual. O objetivo deste trabalho foi avaliar a biocompatibilidade e a atividade antimicrobiana da matriz de hidrogel associado ao extrato romã e ao antimicrobiano ciprofloxacino. Para isso, foram realizadas análises microbiológicas sobre Enterococcus faecalis (ATCC 4083) em cultura planctônica, por meio d ensaio de microdiluição em caldo e em biofilme pelo teste de MTT. Para os ensaios da biocompatibilidade, foi utilizada cultura de macrófagos (RAW 264.7). A citotoxicidade foi avaliada pelo ensaio de MTT e a genotoxicidade foi realizado pelo teste de micronúcleo. A análise estatística foi realizada pelos testes ANOVA e Tukey, adotando o nível de significância 5%. Os resultados demonstraram que a matriz de hidrogel associado ao extrato de romã não apresentou atividade antimicrobiana para a cultura planctônica como para o biofilme de E. faecalis. Porém, não foi citotóxico e genotóxico para RAW 264.7. Por outro lado, o antimicrobiano ciprofloxacino apresentou atividade antimicrobiana sobre E. faecalis,além de ter apresentado efeitos citotóxico e genotóxico para os macrófagos. Com isso, foi possível concluir que o extrato de romã associado ou não a matriz de hidrogel apresenta ausência de citotóxico, genotóxico e efeito antimicrobiano. The hydrogel matrix is a three-dimensional peptide nanofiber biomaterial that induces cell growth, migration and proliferation, thus favoring tissue regeneration. The objective of this work was to evaluate the biocompatibility and antimicrobial activity of the hydrogel matrix associated with the pomegranate extract and the antimicrobial ciprofloxacin. For this, microbiological analyzes on Enterococcus faecalis (ATCC 4083) were carried out in planktonic culture, by means of the microdilution test in broth and in biofilm by the MTT test. For the biocompatibility assays, macrophage culture (RAW 264.7) was used. Cytotoxicity was assessed by the MTT assay and genotoxicity was performed by the micronucleus test. Statistical analysis was performed by the ANOVA and Tukey tests, adopting the significance level 5%. The results showed that the hydrogel matrix associated with the pomegranate extract did not show antimicrobial activity for the planktonic culture as for the E. faecalis biofilm. However, it was not cytotoxic and genotoxic for RAW 264.7. On the other hand, the antimicrobial ciprofloxacin showed antimicrobial activity on E. faecalis, besides having cytotoxic and genotoxic effects for the macrophages. With this, it was possible to conclude that the pomegranate extract associated or not with the hydrogel matrix shows absence of cytotoxic, genotoxic and antimicrobial effect.
- Published
- 2017
5. Análise da citotoxicidade e genocitoxicidade da Aloe vera associada a medicamento endodôntico e fotobiomodulação a laser
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Carvalho, Nayane Chagas, Ribeiro, Maria Amália Gonzaga, and Camargo, Samira Esteves Afonso
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ODONTOLOGIA [CIENCIAS DA SAUDE] ,Genotoxicidade ,Citotoxicidade ,Cytotoxicity ,Endodontia ,Fotobiomodulação a laser ,Odontologia ,Aloe vera ,Endodontic drugs ,Testes de toxicidade ,Laser photobiomodulation ,Medicamentos endodônticos ,Medicamentos ,Genotoxicity ,Babosa (planta) - Abstract
This study aims to evaluate the effect of a drug in order to avoid the appearance of side effects of human pulp fibroblasts. The groups were divided into: CTR with culture medium with fibroblasts; CL, FTL only; AA, Aloe vera with distilled water; AL, Aloe vera with distilled water and FTL; HA, calcium hydroxide P.A. With distilled water; HL, calcium hydroxide P.A. With distilled water and FTL; HAA, calcium hydroxide P.A. With Aloe vera and distilled water; HAL, calcium hydroxide P.A. With Aloe vera, distilled water and FTL. Cytotoxicity evaluation was performed with the MTT reagent at 24, 48 and 72 h, for the evaluation of genotoxicity the micronucleus test was used in 24 h. At 24 h, the CL group presented a greater media of cellular viability, and the HA showed lower mean but stimulated greater number of cell division. At 48 h, the CL group presented a higher medium of cellular viability of high genotoxicity, and the HAL showed lower mean. The AL group showed a higher percentage of surviving cells in 72 h with statistic different from the HA and HL groups (p
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- 2016
6. Citotoxicidade e genotoxicidade de cimentos endodônticos
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Bin, Claudia Villela [UNESP], Universidade Estadual Paulista (Unesp), Valera, Marcia Carneiro [UNESP], and Camargo, Samira Esteves Afonso [UNESP]
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Dental pulp ,Genotoxicidade ,Citotoxicidade ,Dental cements ,Dental materials ,Materiais dentários ,Cytotoxicity ,Biocompatibilidade ,Polpa dentaria ,Biocompatibility ,Cimentos dentarios ,Genotoxicity - Abstract
Made available in DSpace on 2014-06-11T19:24:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-06-10Bitstream added on 2014-06-13T19:31:20Z : No. of bitstreams: 1 bin_cv_me_sjc.pdf: 1076251 bytes, checksum: a167eb57ce7c6168b48f17de4f6795bc (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O MTA é um cimento amplamente utilizado na endodontia devido ao potencial indutor da mineralização, sendo empregado em procedimentos de proteção do complexo dentino/pulpar, retrobturação, perfurações radiculares e de furca e em dentes com rizogênese incompleta. Inicialmente esse material não foi preconizado como cimento obturador de canais radiculares. Entretanto, recentemente foi criado um cimento endodôntico resinoso contendo MTA (MTA Fillapex) com essa indicação, porém não há estudos sobre este novo material. Como o MTA é utilizado sobre os tecidos periodontal, ósseo e pulpar, é importante conhecer seus efeitos citotóxicos e genotóxicos, uma vez que, alguns cimentos endodônticos podem liberar componentes tóxicos, prejudicando o processo de regeneração e reparação dos tecidos no qual entram em contato. Assim, o objetivo deste trabalho foi avaliar a citotoxicidade e genotoxicidade do MTA obturador (Fillapex) comparando-o com o MTA reparador e com o AH Plus. Fibroblastos de hamster Chinês (V79) foram colocados em contato com diferentes diluições de meios de cultura previamente expostos a esses materiais. A citotoxicidade foi avaliada através do ensaio de MTT, em espectrofotômetro, para a verificação da taxa de viabilidade celular. A genotoxicidade foi avaliada pela formação de micronúcleos (MNT), para detecção de mutações cromossômicas. A taxa de sobrevivência celular e o número de micronúcleos antes e após a exposição aos cimentos foram estatisticamente analisados pelo teste de Kruskal-Wallis e teste de Dunn (p
- Published
- 2011
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