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3 results on '"C. Procaccianti"'

1. Whole genome analysis and microRNAs regulation in HepG2 cells exposed to cadmium.

Author

Fabbri M, Urani C, Sacco MG, Procaccianti C, and Gribaldo L

Subjects
Gene Expression Profiling, Hep G2 Cells, Humans, MicroRNAs genetics, Principal Component Analysis, Cadmium toxicity, Gene Expression Regulation drug effects, Genome, Hepatocytes drug effects, Hepatocytes metabolism, MicroRNAs metabolism
Abstract

Cadmium (Cd) is a metal known to be toxic and carcinogenic, but its mechanism of action remains to be fully elucidated. We investigated the gene expression modulation in the human hepatoma cell line HepG2 after exposure to 2 μM and 10 μM Cd using an Agilent microarray. Furthermore, we evaluated the microRNA modulation after exposure to 10 μM Cd with a Low Density Array. At the low concentration only eleven genes belonging to the metallothionein familiy were regulated. At the higher concentration the pathway enrichment analysis for the 536 up-regulated genes showed a large number of pathways related to cancer, whereas the 424 down-regulated genes were enriched on pathways correlated to liver function. A large percentage of modified microRNAs belonged to the let-7 family, which is considered to have oncosuppressor functions. Several pathways connected to cancer were regulated at the transcription level, and miRNAs had a potential impact on the modulation of this regulation.

Published
2012
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2. Whole genome and microRNA analysis in a human hepatoma cell line exposed to Cd

Author

C. Procaccianti, M.G. Saccoa, Chiara Urani, Marco Fabbri, Laura Gribaldo, Gribaldo, L, Fabbri, M, Sacco, M, Procaccianti, C, and Urani, C

Subjects
Genetics, Hepatoma cell line, cadmium, whole genome analysis, microRNA, carcinogenesis, environmental contamination, microRNA, General Medicine, Computational biology, Biology, Toxicology, Genome, BIO/06 - ANATOMIA COMPARATA E CITOLOGIA
Published
2011

3. Whole genome analysis and micrornas regulation in HepG2 cells exposed to cadmium

Author

C. Procaccianti, Maria Grazia Sacco, Chiara Urani, Marco Fabbri, Laura Gribaldo, Fabbri, M, Urani, C, Sacco, M, Procaccianti, C, and Gribaldo, L

Subjects
Pharmacology, cadmium, gene ontology, HepG2 cells, pathway mapping, toxicogenomics, Principal Component Analysis, Genome, Microarray, Gene Expression Profiling, General Medicine, Hep G2 Cells, Biology, Bioinformatics, Cell biology, Gene expression profiling, Medical Laboratory Technology, MicroRNAs, Gene Expression Regulation, Gene expression, microRNA, Hepatocytes, Metallothionein, Humans, Liver function, Gene, Carcinogen, BIO/06 - ANATOMIA COMPARATA E CITOLOGIA, Cadmium
Abstract

Cadmium (Cd) is a metal known to be toxic and carcinogenic, but its mechanism of action remains to be fully elucidated. We investigated the gene expression modulation in the human hepatoma cell line HepG2 after exposure to 2 μM and 10 μM Cd using an Agilent microarray. Furthermore, we evaluated the microRNA modulation after exposure to 10 μM Cd with a Low Density Array. At the low concentration only eleven genes belonging to the metallothionein familiy were regulated. At the higher concentration the pathway enrichment analysis for the 536 up-regulated genes showed a large number of pathways related to cancer, whereas the 424 down-regulated genes were enriched on pathways correlated to liver function. A large percentage of modified microRNAs belonged to the let-7 family, which is considered to have oncosuppressor functions. Several pathways connected to cancer were regulated at the transcription level, and miRNAs had a potential impact on the modulation of this regulation.

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