1. Isolation and characterization of two Staphylococcus aureus lytic bacteriophages "Huma" and "Simurgh".
- Author
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Sharifi F, Montaseri M, Yousefi MH, Shekarforoush SS, Berizi E, Wagemans J, Vallino M, and Hosseinzadeh S
- Subjects
- Humans, Base Composition, Podoviridae genetics, Podoviridae isolation & purification, Podoviridae classification, Podoviridae physiology, HT29 Cells, Genome Size, Staphylococcus Phages genetics, Staphylococcus Phages physiology, Staphylococcus Phages isolation & purification, Staphylococcus aureus virology, Staphylococcus aureus genetics, Genome, Viral
- Abstract
Nowadays finding the new antimicrobials is necessary due to the emerging of multidrug resistant strains. The present study aimed to isolate and characterize bacteriophages against S. aureus. Strains Huma and Simurgh were the two podovirus morphology phages which isolated and then characterized. Huma and Simurgh had a genome size of 16,853 and 17,245 bp, respectively and both were Rosenblumvirus with G + C content of 29%. No lysogeny-related genes, nor virulence genes were identified in their genomes. They were lytic only against two out of four S. aureus strains. They also were able to inhibit S. aureus for 8 h in-vitro. Both showed a rapid adsorption. Huma and Simurgh had the latent period of 80 and 60 m and the burst sizes of 45 and 40 PFU/ml and also, they showed very low cell toxicity of 1.23%-1.79% on HT-29 cells, respectively. Thus, they can be considered potential candidates for biocontrol applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
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