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Your search keyword '"Constantinidou C"' showing total 5 results
Start Over Author "Constantinidou C" Topic genome, bacterial
5 results on '"Constantinidou C"'

1. Genome sequencing in clinical microbiology.

Author

Chan JZ, Pallen MJ, Oppenheim B, and Constantinidou C

Subjects
Humans, Precision Medicine, Bacterial Infections genetics, Bacterial Infections microbiology, Bacteriological Techniques methods, Chromosome Mapping methods, Genetic Testing methods, Genome, Bacterial genetics, Sequence Analysis, DNA methods
Published
2012
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2. High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity.

Author

Loman NJ, Constantinidou C, Chan JZ, Halachev M, Sergeant M, Penn CW, Robinson ER, and Pallen MJ

Subjects
Genetics, Microbial trends, Molecular Biology trends, DNA, Bacterial chemistry, DNA, Bacterial genetics, Genome, Bacterial, High-Throughput Screening Assays
Abstract

Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is 'too cheap to metre' might change the face of microbiology forever.

Published
2012
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3. Complete genome sequence of the Crohn's disease-associated adherent-invasive Escherichia coli strain HM605.

Author

Clarke DJ, Chaudhuri RR, Martin HM, Campbell BJ, Rhodes JM, Constantinidou C, Pallen MJ, Loman NJ, Cunningham AF, Browning DF, and Henderson IR

Subjects
Chromosomes, Bacterial, Colon microbiology, Colon pathology, Crohn Disease pathology, Epithelial Cells microbiology, Humans, Intestinal Mucosa microbiology, Intestinal Mucosa pathology, Molecular Sequence Data, Plasmids, Bacterial Adhesion, Crohn Disease microbiology, Escherichia coli genetics, Escherichia coli isolation & purification, Genome, Bacterial
Abstract

Adherent-invasive Escherichia coli strains are increasingly being associated with intestinal pathologies. Here we present the genome sequence of E. coli HM605, a strain isolated from colonic biopsy specimens of a patient with Crohn's disease.

Published
2011
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4. Genome sequences of three Acinetobacter baumannii strains assigned to the multilocus sequence typing genotypes ST2, ST25, and ST78.

Author

Zarrilli R, Giannouli M, Rocco F, Loman NJ, Haines AS, Constantinidou C, Pallen MJ, Triassi M, and Di Nocera PP

Subjects
Acinetobacter baumannii drug effects, Anti-Bacterial Agents pharmacology, Drug Resistance, Multiple, Bacterial, Molecular Sequence Data, Acinetobacter baumannii classification, Acinetobacter baumannii genetics, Genome, Bacterial, Genotype, Multilocus Sequence Typing
Abstract

Acinetobacter baumannii is an emerging opportunistic gram-negative pathogen responsible for hospital-acquired infections. A. baumannii epidemics described in Europe and worldwide were caused by a limited number of genotypic clusters of multidrug-resistant strains. Here, we report the availability of draft genome sequences for three multidrug-resistant A. baumannii strains assigned to multilocus sequence typing genotypes ST2, ST25, and ST78 that were more frequently isolated during outbreaks occurred in Greece, Italy, Lebanon, and Turkey.

Published
2011
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5. Genomic studies with Escherichia coli MelR protein: applications of chromatin immunoprecipitation and microarrays.

Author

Grainger DC, Overton TW, Reppas N, Wade JT, Tamai E, Hobman JL, Constantinidou C, Struhl K, Church G, and Busby SJ

Subjects
Chromatin, DNA-Binding Proteins genetics, DNA-Directed RNA Polymerases genetics, DNA-Directed RNA Polymerases metabolism, Escherichia coli genetics, Escherichia coli Proteins genetics, Gene Expression Profiling, Promoter Regions, Genetic, Protein Binding, Symporters genetics, Symporters metabolism, Trans-Activators genetics, DNA-Binding Proteins metabolism, Escherichia coli metabolism, Escherichia coli Proteins metabolism, Gene Expression Regulation, Bacterial, Genome, Bacterial, Melibiose metabolism, Oligonucleotide Array Sequence Analysis methods, Precipitin Tests methods, Trans-Activators metabolism
Abstract

Escherichia coli MelR protein is a transcription activator that is essential for melibiose-dependent expression of the melAB genes. We have used chromatin immunoprecipitation to study the binding of MelR and RNA polymerase to the melAB promoter in vivo. Our results show that MelR is associated with promoter DNA, both in the absence and presence of the inducer melibiose. In contrast, RNA polymerase is recruited to the melAB promoter only in the presence of inducer. The MelR DK261 positive control mutant binds to the melAB promoter but cannot recruit RNA polymerase. Further analysis of immunoprecipitated DNA, by using an Affymetrix GeneChip array, showed that the melAB promoter is the major, if not the sole, target in E. coli for MelR. This was confirmed by a transcriptomics experiment to analyze RNA in cells either with or without melR.

Published
2004
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