1. Differential regulation of RNA polymerase III genes during liver regeneration
- Author
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Meghdad Yeganeh, Cristian Carmeli, Dominic Villeneuve, Mauro Delorenzi, Winship Herr, Leonor Rib, Nouria Hernandez, Viviane Praz, Nicolas Guex, CycliX consortium, Hernandez, N., Delorenzi, M., Deplancke, B., Desvergne, B., Guex, N., Herr, W., Naef, F., Rougemont, J., Schibler, U., Andersin, T., Cousin, P., Gilardi, F., Gos, P., Lammers, F., Lopes, M., Mange, F., Minocha, S., Raghav, S., Villeneuve, D., Fabbretti, R., Vlegel, V., Xenarios, I., Migliavacca, E., Praz, V., David, F., Jarosz, Y., Kuznetsov, D., Liechti, R., Martin, O., Delafontaine, J., Cajan, J., Carmeli, C., Gustafson, K., Krier, I., Leleu, M., Molina, N., Naldi, A., Rib, L., Sobel, J., Symul, L., Bounova, G., and Jacquet, P.
- Subjects
Chromatin Immunoprecipitation ,Transcription, Genetic ,DNA polymerase ,DNA polymerase II ,viruses ,RNA polymerase II ,RNA polymerase III ,Histones ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Transcription (biology) ,Genetics ,Animals ,Hepatectomy ,Humans ,Gene ,030304 developmental biology ,0303 health sciences ,biology ,Cell Cycle ,Gene regulation, Chromatin and Epigenetics ,Gene Expression Regulation, Developmental ,RNA Polymerase III ,Histone-Lysine N-Methyltransferase ,Liver regeneration ,Liver Regeneration ,Housekeeping gene ,Cell biology ,Liver ,biology.protein ,RNA Polymerase II ,Cell Division ,030217 neurology & neurosurgery ,Protein Binding - Abstract
Mouse liver regeneration after partial hepatectomy involves cells in the remaining tissue synchronously entering the cell division cycle. We have used this system and H3K4me3, Pol II and Pol III profiling to characterize adaptations in Pol III transcription. Our results broadly define a class of genes close to H3K4me3 and Pol II peaks, whose Pol III occupancy is high and stable, and another class, distant from Pol II peaks, whose Pol III occupancy strongly increases after partial hepatectomy. Pol III regulation in the liver thus entails both highly expressed housekeeping genes and genes whose expression can adapt to increased demand.
- Published
- 2019