Search

Amaranthus is one of the underutilized species distributed and utilized worldwide. In the study was used ISSR approach to analyse intra and inter-specific variability of 16 Amarathus caudatus, 19 A. cruentus and 21 A. hypochodriacus accessions. Using of 11 ISSR primers, fragments levels ranged from 15 up to the 24 were obtained. In the dendrogram based on ISSR data set individual species separation is observed except of three accessions. Two A. caudatus genotypes originated from India clustered with A. hypochondriacus accessions and one A. hypochondriacus genotype originated from Nepal clustered with A. cruentus accessions. Average similarity index among all 55 analysed genotypes ranged from 0,154 to 1,000 with a mean of 0,522. Highest intaspecific variability is reported for A. hypochondriacus accessions when comparing to the A. caudatus or A. cruentus. This study has demonstared, that a single primer marker system as ISSR is able to generate a sufficient level of informative characters for intra and inter-specific molecular analysis of Amaranthus genus. [ABSTRACT FROM AUTHOR]

Molecular markers ISSR were used to analyse γ-irradiated mutants of amaranth Ficha cultivar and K-433 hybrid; tested six microsatellite - primed PCR markers. First analysis of gamma-irradiated amaranth mutant lines was done using the ISSR. In the study, primers that distinguish the mutant lines from control plant were reported as well as primer with the differentiation ability for all analysed mutant lines. The very specific changes in the mutant lines' non-coding regions based on inter microsatellite length polymorphism were analysed. Mutant lines of the Ficha cultivar (C15, C26, C27, C82, C236) shared a genetic dissimilarity of 0.16 and their ISSR profiles were more similar to the Ficha than those of K-433 hybrid mutant lines. The K-433 mutant lines (D54, D279, D282) shared genetic dissimilarity of 0.22 but are more distinct to their control plant as a whole, as those of the Ficha mutant lines. Different ISSR fingerprints patters of the mutant lines when compared to the Ficha cultivar and K-433 hybrid ISSR specific profiles may be part of a consequence of the complex response of the intergenic space of mutant lines to the gamma-radiance. [ABSTRACT FROM AUTHOR]