8 results on '"Henrique Marques-Souza"'
Search Results
2. Embryo size regulates the timing and mechanism of pluripotent tissue morphogenesis
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Viviane Souza Rosa, Lorenzo Carlo Orietti, Marta N. Shahbazi, Christos Kyprianou, Francesco Antonica, Magdalena Zernicka-Goetz, Henrique Marques-Souza, William Mansfield, Zernicka-Goetz, Magdalena [0000-0002-7004-2471], and Apollo - University of Cambridge Repository
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0301 basic medicine ,animal structures ,Time Factors ,Morphogenesis ,embryo ,morphogenesis ,Cell fate determination ,Biology ,Biochemistry ,Exocytosis ,Article ,lumenogenesis ,03 medical and health sciences ,0302 clinical medicine ,epithelial tissue ,Embryonic morphogenesis ,mouse embryogenesis ,regulative development ,apoptosis ,size regulation ,embryonic stem cells ,implantation ,Genetics ,medicine ,Animals ,Amnion ,Cell Aggregation ,Basement membrane ,Embryogenesis ,Embryo ,Cell Biology ,Organ Size ,Embryo, Mammalian ,Embryonic stem cell ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,embryonic structures ,Mice, Inbred CBA ,Female ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Summary Mammalian embryogenesis is a paradigm of regulative development as mouse embryos show plasticity in the regulation of cell fate, cell number, and tissue morphogenesis. However, the mechanisms behind embryo plasticity remain largely unknown. Here, we determine how mouse embryos respond to an increase in cell numbers to regulate the timing and mechanism of embryonic morphogenesis, leading to the formation of the pro-amniotic cavity. Using embryos and embryonic stem cell aggregates of different size, we show that while pro-amniotic cavity formation in normal-sized embryos is achieved through basement membrane-induced polarization and exocytosis, cavity formation of increased-size embryos is delayed and achieved through apoptosis of cells that lack contact with the basement membrane. Importantly, blocking apoptosis, both genetically and pharmacologically, alters pro-amniotic cavity formation but does not affect size regulation in enlarged embryos. We conclude that the regulation of embryonic size and morphogenesis, albeit concomitant, have distinct molecular underpinnings., Graphical abstract, Highlights • Increased-size embryos present a delay in embryonic morphogenesis • Embryo size determines the mechanism of pro-amniotic cavity formation • Apoptosis is required for the formation of a single cavity in enlarged embryos • Cavity formation and size regulation have distinct molecular underpinnings, In this article Shahbazi, Zernicka-Goetz, and colleagues explore how increasing embryo size affects the mechanism and timing of embryonic morphogenesis. Enlarged embryos present a delay in pro-amniotic cavity formation, an essential morphogenetic event that happens concomitantly with the regulation of embryo size. However, whereas pro-amniotic cavity formation requires apoptosis of embryonic cells lacking basement membrane contact, size regulation occurs independently of cell death.
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- 2020
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3. Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure
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Gisela de Aragão Umbuzeiro, Henrique Marques-Souza, Augusto Ducati Luchessi, Karina Danielle Pereira, Theodore B. Henry, Vagner Katsumi Okura, Mariana Coletty Artal, Universidade de São Paulo (USP), Universidade Estadual de Campinas (UNICAMP), Universidade Estadual Paulista (Unesp), Heriot Watt Univ, and Univ Tennessee
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Male ,Silver ,010504 meteorology & atmospheric sciences ,Health, Toxicology and Mutagenesis ,ved/biology.organism_classification_rank.species ,Metal Nanoparticles ,RNA-Seq ,Context (language use) ,010501 environmental sciences ,Biology ,Ecotoxicology ,Toxicology ,01 natural sciences ,Transcriptome ,chemistry.chemical_compound ,Glutathione-S-Transferase ,Animals ,Amphipoda ,Model organism ,Gene ,0105 earth and related environmental sciences ,Genetics ,ved/biology ,Gene Expression Profiling ,RT-qPCR ,Amphipod ,General Medicine ,biology.organism_classification ,Pollution ,chemistry ,Female ,Gene expression ,Toxicant ,Parhyale hawaiensis - Abstract
Made available in DSpace on 2020-12-10T17:31:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Analysis of the transcriptome of organisms exposed to toxicants offers new insights for ecotoxicology, but further research is needed to enhance interpretation of results and effectively incorporate them into useful environmental risk assessments. Factors that must be clarified to improve use of transcriptomics include assessment of the effect of organism sex within the context of toxicant exposure. Amphipods are well recognized as model organisms for toxicity evaluation because of their sensitivity and amenability to laboratory conditions. To investigate whether response to metals in crustaceans differs according to sex we analyzed the amphipod Parhyale hawaiensis after exposure to AgCl and Ag nanoparticles (AgNP) via contaminated food. Gene specific analysis and whole genome transcriptional profile of male and female organisms were performed by both RT-qPCR and RNA-seq. We observed that expression of transcripts of genes glutathione transferase (GST) did not differ among AgCl and AgNP treatments. Significant differences between males and females were observed after exposure to AgCl and AgNP. Males presented twice the number of differentially expressed genes in comparison to females, and more differentially expressed were observed after exposure to AgNP than AgCl treatments in both sexes. The genes that had the greatest change in expression relative to control were those genes related to peptidase and catalytic activity and chitin and carbohydrate metabolic processes. Our study is the first to demonstrate sex specific differences in the transcriptomes of amphipods upon exposure to toxicants and emphasizes the importance of considering gender in ecotoxicology. (C) 2020 Elsevier Ltd. All rights reserved. Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508000 Sao Paulo, Brazil Univ Estadual Campinas, Sch Technol, BR-13484332 Limeira, SP, Brazil Univ Estadual Campinas, Sch Appl Sci, Lab Biotechnol, BR-13484350 Limeira, SP, Brazil Sao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil Univ Estadual Campinas, Life Sci Core Facil LaCTAD, BR-13083886 Campinas, SP, Brazil Heriot Watt Univ, Sch Energy Geosci Infrastruct & Soc, Inst Life & Earth Sci, Edinburgh EH14 4AS, Midlothian, Scotland Univ Tennessee, Ctr Environm Biotechnol, 676 Dabney Hall,1416 Circle Dr, Knoxville, TN 37996 USA Univ Estadual Campinas, Dept Biochem & Tissue Biol, BR-13083970 Campinas, SP, Brazil Sao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil FAPESP: 2014/08829-7 CNPq: 400362/2014-7 CAPES: 001
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- 2020
4. RNA interference as a gene silencing tool to control Tuta absoluta in tomato (Solanum lycopersicum)
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Guilherme Oliveira Barbosa, Joni Esrom Lima, Eric Lam, Lázaro Eustáquio Pereira Peres, Roberto de Almeida Camargo, Antonio Figueira, Isabella Presotto Possignolo, and Henrique Marques-Souza
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0301 basic medicine ,TRAÇAS (CONTROLE) ,lcsh:Medicine ,Genetically modified crops ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Transgenic ,03 medical and health sciences ,Pest control ,RNA interference ,Botany ,Gene silencing ,Gene ,Genetics ,General Neuroscience ,fungi ,lcsh:R ,RNA ,food and beverages ,dsRNA delivery ,General Medicine ,biology.organism_classification ,RNA silencing ,030104 developmental biology ,RNAi ,Tuta absoluta ,Solanum ,dsRNA uptake ,General Agricultural and Biological Sciences - Abstract
RNA interference (RNAi), a gene-silencing mechanism that involves providing double-stranded RNA molecules that match a specific target gene sequence, is now widely used in functional genetic studies. The potential application of RNAi-mediated control of agricultural insect pests has rapidly become evident. The production of transgenic plants expressing dsRNA molecules that target essential insect genes could provide a means of specific gene silencing in larvae that feed on these plants, resulting in larval phenotypes that range from loss of appetite to death. In this report, we show that the tomato leafminer (Tuta absoluta), a major threat to commercial tomato production, can be targeted by RNAi. We selected two target genes (Vacuolar ATPase-AandArginine kinase) based on the RNAi response reported for these genes in other pest species. In view of the lack of an artificial diet forT. absoluta, we used two approaches to deliver dsRNA into tomato leaflets. The first approach was based on the uptake of dsRNA by leaflets and the second was based on “in planta-induced transient gene silencing” (PITGS), a well-established method for silencing plant genes, used here for the first time to deliverin planta-transcribed dsRNA to target insect genes.Tuta absolutalarvae that fed on leaves containing dsRNA of the target genes showed an ∼60% reduction in target gene transcript accumulation, an increase in larval mortality and less leaf damage. We then generated transgenic ‘Micro-Tom’ tomato plants that expressed hairpin sequences for both genes and observed a reduction in foliar damage byT. absolutain these plants. Our results demonstrate the feasibility of RNAi as an alternative method for controlling this critical tomato pest.
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- 2016
5. De novo transcriptome assembly and analysis to identify potential gene targets for RNAi-mediated control of the tomato leafminer (Tuta absoluta)
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Roberto de Almeida Camargo, Flavia de Moura Manoel Bento, Henrique Marques-Souza, Roberto H. Herai, Joni Esrom Lima, Luana Novaes Santos, and Antonio Figueira
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De novo transcriptome assembly ,RNA-Seq ,Genes, Insect ,Biology ,Moths ,Insect Control ,Transcriptome ,Solanum lycopersicum ,RNA interference ,Hormone synthesis ,Genetics ,Animals ,Cluster Analysis ,Gene Silencing ,RNA, Messenger ,Gene ,Solanaceae ,Gene Library ,Gene knockdown ,Base Composition ,Gene Expression Profiling ,fungi ,food and beverages ,Computational Biology ,High-Throughput Nucleotide Sequencing ,Reproducibility of Results ,Molecular Sequence Annotation ,biology.organism_classification ,Hormones ,Gelechiidae ,Lepidoptera ,RNA silencing ,Gene Expression Regulation ,Tuta absoluta ,RNA Interference ,RNA-seq ,Biotechnology ,Research Article - Abstract
Background Providing double-stranded RNA (dsRNA) to insects has been proven to silence target genes, and this approach has emerged as a potential method to control agricultural pests by engineering plants to express insect dsRNAs. A critical step of this technology is the screening of effective target genes essential for insect development and/or survival. The tomato leafminer (Tuta absoluta Meyrick) is a major Solanum lycopersicum (tomato) pest that causes significant yield losses and has recently invaded Europe, from where it is spreading at an alarming rate. To explore RNA interference (RNAi) against T. absoluta, sequence information on potential target genes is necessary, but only a few sequences are available in public databases. Results We sequenced six libraries from RNA samples from eggs, adults, and larvae at four stages, obtaining an overall total of around 245 million reads. The assembled T. absoluta transcriptome contained 93,477 contigs with an average size of 1,574 bp, 59.8 % of which presented positive Blast hits, with 19,995 (21.4 %) annotated by gene ontology. From the transcriptome, most of the core genes of the RNAi mechanism of Lepidoptera were identified indicating the potential suitability of T. absoluta for gene silencing. No contigs displayed significant similarity with a RNA-dependent RNA Polymerase. Genes from the juvenile hormone and ecdysteroid biosynthetic pathways were identified, representing potential target genes for systemic silencing. Comparisons of transcript profiles among stages revealed 1,577 genes differentially expressed at earlier larval stages, from which potential gene targets were identified. Five of these genes were evaluated using in vitro transcribed dsRNA absorbed by tomato leaflets, which were fed to 1st instar T. absoluta larvae, resulting in significant reduction of larval body weight while exhibiting significant knockdown for three of the genes. Conclusions The transcriptome we generated represents a valuable genomic resource for screening potential gene targets that affect the development or survival of T. absoluta larvae. Five novel genes that showed greater expression at the 1st larval stage were demonstrated to be effective potential RNAi targets by reducing larval weight and can be considered good candidates for use in RNAi-mediated crop protection. Electronic supplementary material The online version of this article (doi:10.1186/s12864-015-1841-5) contains supplementary material, which is available to authorized users.
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- 2015
6. Delimiting the conserved features of hunchback function for the trunk organization of insects
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Diethard Tautz, Manuel Aranda, and Henrique Marques-Souza
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animal structures ,Embryo, Nonmammalian ,Biology ,Krüppel ,Animals ,Hox gene ,Molecular Biology ,Gene ,Gap gene ,Ultrabithorax ,Regulation of gene expression ,Genetics ,Homeodomain Proteins ,Tribolium ,Gene Expression Profiling ,fungi ,Gene Expression Regulation, Developmental ,Phenotype ,Cell biology ,DNA-Binding Proteins ,Larva ,embryonic structures ,Drosophila ,RNA Interference ,Blastoderm ,Developmental Biology ,Transcription Factors - Abstract
The gap gene hunchback in Drosophila acts during syncytial blastoderm stage via a short-range gradient and concentration-dependent activation or repression of target genes. Orthologues of hunchback can be easily found in other insects, but it has been unclear how well its functions are conserved. The segmentation process in most insect embryos occurs under cellular conditions, which should not allow the formation of diffusion-controlled transcription factor gradients. We have studied here in detail the function of hunchback in the short germ embryo of Tribolium using parental RNAi and interaction with possible target genes. We find that hunchback is a major regulator of the trunk gap genes and Hox genes in Tribolium, but may only indirectly be required to regulate other segmentation genes. The core function of hunchback appears to be the setting of the Ultrabithoraxexpression border via a repression effect, and the activation of the Krüppel expression domain. These regulatory effects are likely to be direct and are conserved between Drosophila and Tribolium. We find no evidence for a classical gap phenotype in the form of loss of segments in the region of expression of hunchback. However, the phenotypic effects in Tribolium are highly comparable with those found for other short germ embryos, i.e. the core functions of hunchback in Tribolium appear to be the same in these other insects, although they are evolutionarily more distant to Tribolium,than Tribolium is to Drosophila. These results allow the disentanglement of the conserved role of hunchback in insects from the derived features that have been acquired in the lineage towards Drosophila. Given that the gap phenotype appears to occur only in long germ embryos and that the main role of hunchback appears to be the regionalization of the embryo, it may be appropriate to revive an alternative name for the class of gap genes, namely `cardinal genes'.
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- 2008
7. A segmentation gene in tribolium produces a polycistronic mRNA that codes for multiple conserved peptides
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Manuel Aranda, Henrique Marques-Souza, Joël Savard, and Diethard Tautz
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Flour beetle ,animal structures ,Embryo, Nonmammalian ,Molecular Sequence Data ,Limb Deformities, Congenital ,General Biochemistry, Genetics and Molecular Biology ,Animals ,Drosophila Proteins ,RNA, Messenger ,Gene ,Gap gene ,Body Patterning ,Genetics ,Messenger RNA ,Gene knockdown ,Tribolium ,biology ,Base Sequence ,Sequence Homology, Amino Acid ,Biochemistry, Genetics and Molecular Biology(all) ,fungi ,Gene Expression Regulation, Developmental ,Embryo ,Extremities ,biology.organism_classification ,Segmentation gene ,Transformation (genetics) ,ras GTPase-Activating Proteins ,Insect Proteins ,Peptides - Abstract
Summary Segmentation genes in insects are required for generating the subdivisions of the early embryo. We describe here a new member of the gap family of segmentation genes in the flour beetle Tribolium , mille-pattes ( mlpt ). mlpt knockdown leads to transformation of the abdominal segments into thoracic segments, providing embryos with up to ten pairs of legs. We show that there are crossregulatory interactions between mlpt and the known gap genes in Tribolium , suggesting that mlpt is itself a gap gene. The mlpt gene reveals an unusual structure, as it encodes a polycistronic mRNA that codes for four peptides. mlpt appears to be the prototype of this previously unknown gene structure in eukaryotes, as we find homologous genes with the same polycistronic arrangement in other insect genomes as well.
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- 2006
8. The role of the segmentation gene hairy in Tribolium
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Manuel Aranda, Till Bayer, Henrique Marques-Souza, and Diethard Tautz
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Embryo, Nonmammalian ,animal structures ,Transcription, Genetic ,Eggs ,Pair-rule gene ,Gene Expression ,Biology ,Segmentation ,RNA interference ,Genetics ,Animals ,Cloning, Molecular ,Phylogeny ,Short germ embryogenesis ,Gene knockdown ,Tribolium ,Germ-band extension ,fungi ,Segmentation gene ,Female ,RNA Interference ,Original Article ,Pair-rule genes ,Blastoderm ,Developmental biology ,Trunk segmentation ,Developmental Biology - Abstract
Hairy stripes in Tribolium are generated during blastoderm and germ band extension, but a direct role for Tc-h in trunk segmentation was not found. We have studied here several aspects of hairy function and expression in Tribolium, to further elucidate its role. First, we show that there is no functional redundancy with other hairy paralogues in Tribolium. Second, we cloned the hairy orthologue from Tribolium confusum and show that its expression mimics that of Tribolium castaneum, implying that stripe expression should be functional in some way. Third, we show that the dynamics of stripe formation in the growth zone is not compatible with an oscillatory mechanism comparable to the one driving the expression of hairy homologues in vertebrates. Fourth, we use parental RNAi experiments to study Tc-h function and we find that mandible and labium are particularly sensitive to loss of Tc-h, reminiscent of a pair-rule function in the head region. In addition, lack of Tc-h leads to cell death in the gnathal region at later embryonic stages, resulting in a detachment of the head. Cell death patterns are also altered in the midline. Finally, we have analysed the effect of Tc-h knockdown on two of the target genes of hairy in Drosophila, namely fushi tarazu and paired. We find that the trunk expression of Tc-h is required to regulate Tc-ftz, although Tc-ftz is itself also not required for trunk segmentation in Tribolium. Our results imply that there is considerable divergence in hairy function between Tribolium and Drosophila. Electronic supplementary material The online version of this article (doi:10.1007/s00427-008-0240-1) contains supplementary material, which is available to authorized users.
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