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Ginger is a vegetable with medicinal and culinary properties widely cultivated in the Southern and Southeastern Brazil. The knowledge of ginger species' genetic variability is essential to direct correctly future studies of conservation and genetic improvement, but in Brazil, little is known about this species' genetic variability. In this study, we analyzed the genetic diversity and structure of 55 Brazilian accessions and 6 Colombian accessions of ginger, using AFLP (Amplified Fragment Length Polymorphism) molecular markers. The molecular characterization was based on 13 primers combinations, which generated an average of 113.5 polymorphic loci. The genetic diversity estimates of Nei (Hj), Shannon-Weiner index (I) and an effective number of alleles (n e ) were greater in the Colombian accessions in relation to the Brazilian accessions. The analysis of molecular variance showed that most of the genetic variation occurred between the two countries while in the Brazilian populations there is no genetic structure and probably each region harbors 100 % of genetic variation found in the samples. The bayesian model-based clustering and the dendrogram using the dissimilarity's coefficient of Jaccard were congruent with each other and showed that the Brazilian accessions are highly similar between themselves, regardless of the geographic region of origin. We suggested that the exploration of the interspecific variability and the introduction of new varieties of Z.officinale are viable alternatives for generating diversity in breeding programs in Brazil. The introduction of new genetic materials will certainly contribute to a higher genetic basis of such crop.

The distribution of organs for renal transplant depends on HLA matching between donor and recipient. This study aimed to characterize the allele and haplotype frequencies of HLA-A, -B, and -DRB1 in a cohort of renal transplant candidates populations in the region of Sao José do Rio Preto (State of São Paulo), to compare the allele frequencies between Caucasian and Black in that region, as well as to compare these frequencies with different Brazilian populations reported. The HLA-A, -B, and -DRB1 allele and haplotypes frequencies were analyzed in a sample of 2.624 individuals and classified according to the ethnic group (2.347 Caucasians and 277 Blacks). The HLA class I (A, B) and class II (DRB1) specificities were determined by complement-dependent microlymphocytotoxic (CDC) and Polymerase Chain Reaction/Sequence Specific Priming (PCR-SSP) methods, respectively. Twenty-one HLA-A, 34 HLA-B and 13 HLA-DRB1 allelic groups were identified. The most frequent alleles for each locus were HLA-A(∗)02, HLA-B(∗)35, and HLA-DRB1(∗)11. The most frequent haplotypes found were A(∗)01 B(∗)08 DRB1(∗)03 among Caucasians and A(∗)29 B(∗)15 DRB1(∗)04 among Blacks. The most common alleles for each locus among RTx were HLA-A(∗)02, HLA-B(∗)35 and HLA-DRB1(∗)11. The haplotypes A(∗)01 B(∗)08 DRB1(∗)03 and A(∗)29 B(∗)44 DRB1(∗)07 prevailed among Caucasians and Blacks, respectively. This study provides the first data on the HLA-A, HLA-B and HLA-DRB1 allele and haplotype frequencies of renal transplant candidates populations in the region of Sao José do Rio Preto., (Copyright © 2016 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)

Centropomus undecimalis (common snook) and Centropomus parallelus (fat snook) have a wide distribution from southern Florida to southern Brazil. Due to their value as a food source, these species have been heavily exploited through predatory fishing, posing a conservation challenge. To assess their genetic diversity and population structure, we used microsatellite markers. Our findings revealed genetic differences among populations of the same species, highlighting the need for targeted conservation efforts. The microsatellite markers proved effective in assessing genetic variability, providing valuable insights for management and conservation. The parameters Ho (observed heterozygosity) and He (expected heterozygosity) were reliable indicators of genetic diversity, and specific loci showed varying allele numbers across populations. Our study contributes to understanding population genetics in these snook species and supports their conservation. Despite not being classified as endangered, genetic differences among populations emphasize the importance of considering population-level characteristics in conservation strategies. This research lays the foundation for future studies and actions aimed at preserving these valuable fish species. In summary, our study demonstrates the significance of microsatellite markers in assessing genetic variability and population structure in common snook and fat snook, informing conservation efforts for these species. [ABSTRACT FROM AUTHOR]

(1) Background: Campomanesia adamantium is an endemic species of the Cerrado and this biome has been under threat by its constant degradation. The genetic diversity of C. adamantium was characterized using species-specific microsatellites in two different time periods and correlations of these parameters of genetic diversity with the land use and land cover data. (2) Methods: We used 10 microsatellite loci to analyze C. adamantium from five populations, collected in 2011 and 2017. Maps of land use and land cover of the collection sites in both years were generated and subsequently correlated with genetic diversity. (3) Results: No significant loss of genetic diversity was observed in the analyzed period and a positive inbreeding coefficient was observed in all populations. AMOVA and STRUCTURE showed that there is no population structure between years and populations. Significant Pearson correlations were observed in 2017 between parameters of genetic diversity and land use and land cover, with a positive correlation between expected heterozygosity and secondary vegetation, and a negative correlation between inbreeding coefficient and exposed soil. (4) Conclusions: There was no decline in genetic diversity from 2011 to 2017. The high rates of the inbreeding coefficient could lead, for the species, in the long run, to an inbreeding depression as the Cerrado fragmentation might cause a population bottleneck. [ABSTRACT FROM AUTHOR]

The essential oil of four populations of Baccharis dracunculifolia was analyzed for their chemical composition by CG/MS and the study of genetic diversity was conducted by analyzing the DNA polymorphism using microsatellite markers. The predominant compounds were E -nerolidol, cis -β-guaiene, germacrene D, E -caryophyllene, limonene, α-pinene, β-myrcene, δ-cadinene, β-pinene and spathulenol. The E -nerolidol substance was the most abundant among the compounds present in the essential oil in all populations. Genetic analysis showed that most of the diversity of B. dracunculifolia was within populations, and the diversity among populations is not expressive. All the populations evaluated in this study were in a process of extensive fragmentation, this might be the main cause of reduced genetic diversity of this specie in Cerrado biome. The comparison between PCA of genotypes and chemodiversity reinforces the unique profile for each population despite the environmental similarity observed and genetic analysis. The results obtained in this work, using microsatellite markers, made it possible to assess the genetic variability in natural populations of B. dracunculifolia. • Baccharis dracunculifolia is the main source of botanical green propolis. • The species is well known by its uses in South America folk medicine. • The essential oil exerts a pleasant aroma reminiscent of rosemary (alecrim-do-campo). • The genetic diversity of populations was assessed by microsatellite markers. • Most of the genetic diversity of Baccharis dracunculifolia was intrapopulation. [ABSTRACT FROM AUTHOR]