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Mungbean yellow mosaic virus (MYMV) is a single-stranded DNA (ssDNA) begomovirus that is the most prevalent threats in Pakistan to mungbean crop. Ten genotypes were selected from 127 genotypes which have already been screened for resistance under field conditions. A total of 38 bands were scored, exhibited high level of polymorphism i.e., 68.42%, with amplification range of (1-7bands). Genetic analysis often primers revealed similarities in the range of 66.60-97.70%. Majority of primers were moderately informative with polymorphism information content (PIC) value (0.00-0.566) and average PIC value of all primers was 0.379. Cluster analysis inferred that genotypes showing resistant response against MYMV were present in un-clustered form in dendrogram. On the basis of phylogenetic analysis NM-2011 has higher genetic difference which reduces to the next branches showed that the susceptible (S) and moderately susceptible (MS) genotypes diverged from resistant genotypes. Diverse field response of mungbean was confirmed even on SSR markers and furthermore it is recommended that molecular markers are effective tags for genetic diversity calculation in mungbean germplasm and could be utilized for the future breeding program regarding mungbean crop. [ABSTRACT FROM AUTHOR]

Bulgaria has long-standing traditions of cultivating oil-bearing roses and is one of the leading producers of rose essential oil and rose water worldwide. We studied the genetic diversity of twenty-two R. damascena accessions from Bulgaria and Saudi Arabia using 10 inter-simple sequence repeat (ISSR) primers. In total, 212 bands were generated, of which 204 were polymorphic and eight monomorphic. High mean values of polymorphic information content (0.75), effective multiplex ratio (8.90), resolving power (32.40), marker index (6.59), and percentage of polymorphic bands (96.26) were obtained, implying significant genetic variability between the studied genotypes. The ISSR-based analyses showed that the level of polymorphism in this crop is appreciably high (Ne = 1.28, I = 0.24, He = 0.16). The coefficient of genetic differentiation among pre-defined groups was 0.46, indicating that 46% of total genetic variability was between groups and 54% was within groups. The method was appropriate for distinguishing individuals with a high level of similarity. The genetic relationships analysed with PCoA and hierarchical clustering analyses clearly separated the group of Bulgarian R. damascena accessions collected from local industrial plantations from the other two pre-defined groups. The clustering of Bulgarian rose cultivars and accessions from Saudi Arabia was independent of their geographical origin. The reported data could further facilitate the management of genetic resources and the development of rose breeding programs. Our study provides the first data on the genetic diversity of R. damascena accessions from Bulgaria using ISSR markers. [ABSTRACT FROM AUTHOR]

The study was conducted to evaluate eight germplasms/varieties of wheat during the rabi season (November-April, 2022--23) using a randomized block design with three replications at the research farm of Agricultural Research Sub-Station (ARSS), Nagaur (Rajasthan), India to estimate the variability parameters and characters association for nine characters. The results were revealed that mean sum of squares due to genotypes showed significant differences for all the nine characters under study, suggested that the genotypes were genetically divergent. The phenotypic coefficient of variation values was higher than genotypic coefficient of variation values for all the nine traits which reflect the influence of environment on the expression of traits. Spike length and number of spikelets were showed higher PCV and medium GCV. High heritability estimates were recorded for all the nine characters. High genetic advance as percentage of mean along with high heritability was observed for number of spikelets, spike length, number of effective tillers plant-1, seed yield and number of grains spike-1. This indicated the existence of lesser environmental influence and prevalence of additive gene action in their expression and these traits possessed high selective value. These characters could be further improved through individual plant selection. Grain yield had highly significant and positive genotypic and phenotypic correlations with plant height, number of effective tillers plant-1, spike length, number of spikelets and number of grains spike-1. These characters can be considered as criteria for selection for higher grain yield as these were mutually and directly associated with grain yield. [ABSTRACT FROM AUTHOR]

Within a species population, the amount of diversification is reflected by its genetic variability as it enumerates the genetical and environmental favour on that population. In terms of this, forty eight okra collections were field investigated in RCB design during 2022 as a summer crop. Significant difference among the collected okra accessions was statistically proven by ANOVA. Per se range for okra traits clarified the elaborate variation for fruit yield and others. As always, GCV was marked as lower than PCV which signalled the error regarding environment. Genetic advance along with heritability was noticed high for most of the traits especially yield and its related components that entrusted the selection of traits on phenotype for new trait based expectations perpetually in okra. [ABSTRACT FROM AUTHOR]

Genetic diversity is referred to as any variation at the phenotypic, DNA or genomic level of an individual, population or species. The appraisal of diversity is important to understand its pattern and evolutionary relationships between germplasms or genotypes, which will aid in sampling the genetic resources in a more systematic manner for conservation and crop improvement. The present study employed 50 simple sequence repeat (SSR) markers linked to the yield and fibre quality/colour traits for estimating the genetic diversity in 33 cotton genotypes of diploid and tetraploid species differing in fibre colour. The diversity analysis was performed in GenAlEx (Ver. 6.41) and Powermarker (Ver. 3.25) while DARwin (Ver. 6.0.21) software was used to establish the phylogenetic relationships following neighbour- -joining (NJ) and unweighted pair group method with arithmetic (UPGMA) mean method. Markers generated 186 polymorphic loci as genotypic data with an average of 3.72 alleles and an average polymorphic information content (PIC) value of 0.59 per SSR locus. The NJ and UPGMA grouped 33 genotypes into three major clusters I, II and III consisting of 21 tetraploid Gossypium hirsutum, 10 G. arboreum coloured and 2 white cotton genotypes, respectively. In the PCA, the first two components (PC1 and PC2) explained 74.69% of the variation and the biplot plotted the 33 genotypes in three groups. The study established the diverse nature of 33 cotton genotypes based on their fibre colour and ploidy level. With confirmation of the prevalent genetic diversity, we suggest that hybridisation can be planned among diverse genotypes to unleash greater variation in the fibre colour or to derive superior cross combinations. [ABSTRACT FROM AUTHOR]

The two recombinant inbred line (RIL) populations developed by crossing Almaly × Avocet S (206 RILs) and Almaly × Anza (162 RILs) were used to detect the novel genomic regions associated with adult plant resistance (APR) and seedling or allstage resistance (ASR) to yellow rust (YR) and leaf rust (LR). The quantitative trait loci (QTLs) were detected through multi-year phenotypic evaluations (2018–2020) and using high-throughput DArTseq genotyping technology. RILs exhibited significant genetic variation with p < 0.001, and the coefficient of variation ranged from 9.79% to 47.99% for both LR and YR in all Environments and stages of evaluations. The heritability is quite high and ranged between 0.47 and 0.98. We identified nine stable QTLs for YR APR on chromosomes 1B, 2A, 2B, 3D, and 4D and four stable QTLs for LR APR on chromosomes 2B, 3B, 4A, and 5A. Furthermore, in silico analysis revealed that the key putative candidate genes such as cytochrome P450, protein kinase-like domain superfamily, zincbinding ribosomal protein, SANT/Myb domain, WRKY transcription factor, nucleotide sugar transporter, and NAC domain superfamily were in the QTL regions and probably involved in the regulation of host response toward pathogen infection. The stable QTLs identified in this study are useful for developing rust-resistant varieties through marker-assisted selection (MAS). [ABSTRACT FROM AUTHOR]

Wamena is notable for producing and exporting high-quality coffee. However, a molecular method based on its polymorphism and not affected by external factors is required since morphological observations alone are frequently insufficient for identifying the coffee. The objective of this study is to identify Wamena Arabica coffee in the Jayawijaya District using simple sequence repeats (SSRs) molecular markers. This research involved several stages, including DNA isolation and purification, PCR SSR amplification with 5 primers, polymorphism, and heterozygosity level analysis. The results analysis proved that 30 alleles of 32 coffee genotypes were successfully amplified, with fragment sizes ranging from 176 to 553bp. The Car M052 locus (0.29%) had the least polymorphism with only two alleles, while the CarM101 locus (0.82%) had the most with 12 alleles, that high and low polymorphism indicated a measure of PIC scores. Heterozygosity analysis showed that the coffee samples were highly heterozygous. And based on the results of the bootstrap analysis, the phenogram shows that the coffee samples are divided into six clusters, with a cophenetic correlation coefficient (r) of 0.948 (excellent fit). This study proved that all SSR loci succeeded in amplifying 30 alleles and could be identified molecularly based on the genetic variation of the Wamena Arabica coffee genotypes in Papua. It seems highly possible that there is a mix of traits through gene flow and exchange between coffee genotypes, and the heterozygosity in the Arabica population on plantations. As a result, it is very important further analysis to confirm the findings. [ABSTRACT FROM AUTHOR]

Date palm has excellent economic value all over the world. Date palm genotypes grown in Pakistan are diverse in nature. The current study aimed to explore the diversity in 50 date palm genotypes collected from two different research stations in Punjab, Pakistan. The study findings are as follows: Dhakki had the highest fruit weight, pulp weight, fruit width and fruit volume. Jaman had a longer fruit length. Eedel Shah and Begum Jangi had greater fruit thickness and fruit perimeter, respectively. Makran showed a higher fruit area. Dedhi showed the highest stone weight, thickness and volume. Halmain had a longer stone length, and Sundari had an extended stone width. Makhi exhibited a higher stone perimeter and area. The Danda genotype had low weight and a small length of the stone. Begum Jangi and Peeli Sundar had small stone width. Seib and Shado had a higher moisture content in their fruits. The fruits of Baidhar and Khudraw-2 genotypes had the highest dry matter and reducing sugars. Champa Kali and Shakri had higher TSS levels in fruits. Halmain had higher juice pH. The fruits of Pathri and Makhi genotypes exhibited higher non-reducing sugars and carotenoids. In this study, a dendrogram was constructed to cluster 50 genotypes into five different clusters based on their physico-chemical characteristics. The correlation matrix and variable plot revealed positive and negative correlations between fruits traits and their biochemical properties conducive to the improvement of desired traits. Principal component analysis (PCA) revealed that Dhakki, Chohara, Baidhar, Karbalaen and Eedel Shah showed a higher genetic diversity; hence, in the scatter plot and biplot, these genotypes deviated from the centre of origin. Physico-chemical characteristics of data palm genotypes indicated a huge diversity among them, which could help select diverse parents, which is important for different breeding purposes. [ABSTRACT FROM AUTHOR]

Wild pomegranate is a valuable edible species in the plant ecosystem of the Hyrcanian forests and the northern plains of the Caspian coast in Iran. The genetic diversity of these wild pomegranates can be effective in pomegranate breeding programs and germplasm conservation. In the present study, morphological diversity in 103 wild pomegranates (Punica granatum L. var. spinosa) in the northeastern area of Iran was studied using 46 traits related to trees, flowers, and fruits. The results showed that the fruit weight ranged from 17.93 to 99.9 g with an average of 48.92 g, the total aril weight ranged from 0.54 g to 64.78 g with an average of 24.25 g, and the weight of 100 arils was between 4.89 and 46.21 with an average of 13.79. The fruit cracking percent, crown shape, aril juiciness, calyx, and corolla colors show a high coefficient of variation (CV > 70%). Based on PCA results, fruit weight and total aril weight, peel weight, and fruit length and diameter were important on determining differences among accessions. In biplot analysis, genotype distribution was determined by two main factors. In cluster analysis, the studied accessions were divided into two different major clusters and two subclusters in each one. The results showed a high diversity of important pomological traits in wild pomegranates such as fruit weight, fruit cracking percent, crown shape, total aril weight, aril juiciness corolla, and calyx color that can be used in breeding programs to improve pomegranate juice quality and marketability. [ABSTRACT FROM AUTHOR]

As one of the oldest fruit trees of the Arabian peninsula, other Middle-Eastern countries, and also North Africa, the date palm (Phoenix dactylifera L.), is highly significant for the economy of the region. Listed as part of UNESCO's Intangible Cultural Heritage of Humanity, the date palm is believed to be the first tree cultivated by human beings, and was probably first harvested for its fruit nearly 7,000 years ago. Initial research efforts in date palm genetics focused on understanding the genetic diversity of date palm germplasm collections and its phylogenetic history, both important prerequisites for plant improvement. Despite various efforts, the center of origin of the date palm is still unclear, although genomic studies suggest two probable domestication events: one in the Middle East and the other in North Africa, with two separate gene pools. The current review covers studies related to omics analyses that have sought to decipher the present genetic diversity of the date palm. With advances and cost reductions in sequencing technologies, rapid progress has been made in the past few years in date palm genomics research. Along with organellar genomes, several reference genomes of the date palm are now available. In addition, several genotypes have been re-sequenced, either to detect single nucleotide polymorphisms (SNPs), or to study domestication and identification of key genes/loci associated with important agronomic traits, such as sex, fruit color, and sugar composition. These genomics research progress has paved the way to perform fast-track and precise germplasm improvement processes in date palm. In this study, we review the advances made in the genetics and genomics of the date palm so as to strategize targeted crop improvement plans for marginal areas of the Middle Eastern peninsula, North Africa, and other parts of the world. [ABSTRACT FROM AUTHOR]

Spring wheat (Triticum aestivum L.) is one of the most imperative staple food crops, with an annual production of 765 million tons globally to feed ∼40% world population. Genetic diversity in available germplasm is crucial for sustainable wheat improvement to ensure global food security. A diversity panel of 184 Pakistani wheat accessions was genotyped using 123,596 high-quality single nucleotide polymorphism (SNP) markers generated by genotyping-by-sequencing with 42% of the SNPs mapped on B, 36% on A, and 22% on D sub-genomes of wheat. Chromosome 2B contains the most SNPs (9,126), whereas 4D has the least (2,660) markers. The mean polymorphic information content, genetic diversity, and major allele frequency of the population were 0.157, 0.1844, and 0.87, respectively. Analysis of molecular variance revealed a higher genetic diversity (80%) within the sub-population than among the sub-populations (20%). The genome-wide linkage disequilibrium was 0.34 Mbp for the whole wheat genome. Among the three subgenomes, A has the highest LD decay value (0.29 Mbp), followed by B (0.2 Mbp) and D (0.07 Mbp) genomes, respectively. The results of population structure, principal coordinate analysis, phylogenetic tree, and kinship analysis also divided the whole population into three clusters comprising 31, 33, and 120 accessions in group 1, group 2, and group 3, respectively. All groups were dominated by the local wheat accessions. Estimation of genetic diversity will be a baseline for the selection of breeding parents for mutations and the genome-wide association and marker-assisted selection studies. [ABSTRACT FROM AUTHOR]

Background: Guava (Psidium guajava L.) is an important cultivated fruit crop in Vietnam due to its commercial value for fresh consumption and especially for fruit processing industry. A large number of diverse guava accessions, both indigenous and exotic, are available in Vietnam but their characterization, which is needed to identify targeted guava accessions for breeding programs, is still limited. Methods: The phenotypic variation and relationship among 40 guava accessions collected across Vietnam were assessed employing leaf and fruit morphological and physiological characterizations. Result: Range of variation for different leaf and fruit parameters were recorded. Significant correlations between leaf and fruit traits relating to yield and fruit quality were also observed implying a possibility of their simultaneous or interchangeable use in selection. Furthermore, the dendrogram generated from agglomeration hierarchical clustering grouped the 40 guava accessions into four major clusters which are highly associated with their origins. The result indicated that existing guava accessions are an important source of genetic diversity and can be used in the guava improvement program in Vietnam. [ABSTRACT FROM AUTHOR]