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RNA interference (RNAi) was investigated with the aim of achieving gene silencing with diverse RNAi platforms that include small interfering RNA (siRNA), short hairpin RNA (shRNA) and antisense oligonucleotides (ASO). Different versions of each system were used to silence the expression of specific subunits of the heterotrimeric signal transducing G-proteins, G alpha i2 and G beta 2, in the RAW 264.7 murine macrophage cell line. The specificity of the different RNA interference (RNAi) platforms was assessed by DNA microarray analysis. Reliable RNAi methodologies against the genes of interest were then developed and applied to functional studies of signaling networks. This study demonstrates a successful knockdown of target genes and shows the potential of RNAi for use in functional studies of signaling molecules. [ABSTRACT FROM AUTHOR]

An abstract of the article "rAAV-2 Transduction of Primitive Human Hematopoietic Stem Cells Capable of Serial Engraftment in Immune Deficient Mice," by Helicia Paz, Leah Santat, Lijing Li, Christie Wong, Stephen J. Forman, K. K. Wong and Saswati Chatterjee is presented.