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Your search keyword '"Tee, Nicole Gui Zhen"' showing total 2 results
Start Over Author "Tee, Nicole Gui Zhen" Topic genetic predisposition to disease
2 results on '"Tee, Nicole Gui Zhen"'

1. WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling.

Author

Chen H, Moreno-Moral A, Pesce F, Devapragash N, Mancini M, Heng EL, Rotival M, Srivastava PK, Harmston N, Shkura K, Rackham OJL, Yu WP, Sun XM, Tee NGZ, Tan ELS, Barton PJR, Felkin LE, Lara-Pezzi E, Angelini G, Beltrami C, Pravenec M, Schafer S, Bottolo L, Hubner N, Emanueli C, Cook SA, and Petretto E

Subjects
Adolescent, Adult, Aged, Animals, Cardiomyopathies genetics, Cardiomyopathies metabolism, Extracellular Matrix Proteins metabolism, Female, Fibrosis genetics, Gene Expression Regulation, Heart Diseases genetics, Heart Diseases metabolism, Humans, Male, Mice, Mice, Transgenic, Middle Aged, Protein Isoforms, Smad2 Protein genetics, Transforming Growth Factor beta metabolism, Ubiquitin-Protein Ligases genetics, Young Adult, Fibrosis metabolism, Gene Regulatory Networks, Genetic Predisposition to Disease genetics, Smad2 Protein metabolism, Ubiquitin-Protein Ligases metabolism
Abstract

Cardiac fibrosis is a final common pathology in inherited and acquired heart diseases that causes cardiac electrical and pump failure. Here, we use systems genetics to identify a pro-fibrotic gene network in the diseased heart and show that this network is regulated by the E3 ubiquitin ligase WWP2, specifically by the WWP2-N terminal isoform. Importantly, the WWP2-regulated pro-fibrotic gene network is conserved across different cardiac diseases characterized by fibrosis: human and murine dilated cardiomyopathy and repaired tetralogy of Fallot. Transgenic mice lacking the N-terminal region of the WWP2 protein show improved cardiac function and reduced myocardial fibrosis in response to pressure overload or myocardial infarction. In primary cardiac fibroblasts, WWP2 positively regulates the expression of pro-fibrotic markers and extracellular matrix genes. TGFβ1 stimulation promotes nuclear translocation of the WWP2 isoforms containing the N-terminal region and their interaction with SMAD2. WWP2 mediates the TGFβ1-induced nucleocytoplasmic shuttling and transcriptional activity of SMAD2.

Published
2019
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2. WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling

Author

Chen, Huimei, Moreno-Moral, Aida, Pesce, Francesco, Devapragash, Nithya, Mancini, Massimiliano, Heng, Ee Ling, Rotival, Maxime, Srivastava, Prashant K, Harmston, Nathan, Shkura, Kirill, Rackham, Owen JL, Yu, Wei-Ping, Sun, Xi-Ming, Tee, Nicole Gui Zhen, Tan, Elisabeth Li Sa, Barton, Paul, Felkin, Leanne E, Lara-Pezzi, Enrique, Angelini, Gianni, Beltrami, Cristina, Pravenec, Michal, Schafer, Sebastian, Bottolo, Leonardo, Hubner, Norbert, Emanueli, Costanza, Cook, Stuart A, and Petretto, Enrico

Subjects
Adult, Male, Extracellular Matrix Proteins, Adolescent, Heart Diseases, Ubiquitin-Protein Ligases, Mice, Transgenic, Smad2 Protein, Middle Aged, Fibrosis, 3. Good health, Mice, Young Adult, Gene Expression Regulation, Transforming Growth Factor beta, Animals, Humans, Protein Isoforms, Female, Gene Regulatory Networks, Genetic Predisposition to Disease, Cardiomyopathies, Aged
Abstract

Cardiac fibrosis is a final common pathology in inherited and acquired heart diseases that causes cardiac electrical and pump failure. Here, we use systems genetics to identify a pro-fibrotic gene network in the diseased heart and show that this network is regulated by the E3 ubiquitin ligase WWP2, specifically by the WWP2-N terminal isoform. Importantly, the WWP2-regulated pro-fibrotic gene network is conserved across different cardiac diseases characterized by fibrosis: human and murine dilated cardiomyopathy and repaired tetralogy of Fallot. Transgenic mice lacking the N-terminal region of the WWP2 protein show improved cardiac function and reduced myocardial fibrosis in response to pressure overload or myocardial infarction. In primary cardiac fibroblasts, WWP2 positively regulates the expression of pro-fibrotic markers and extracellular matrix genes. TGFβ1 stimulation promotes nuclear translocation of the WWP2 isoforms containing the N-terminal region and their interaction with SMAD2. WWP2 mediates the TGFβ1-induced nucleocytoplasmic shuttling and transcriptional activity of SMAD2.

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