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Your search keyword '"Synthetic Biology standards"' showing total 6 results
Start Over Descriptor "Synthetic Biology standards" Topic genetic engineering
6 results on '"Synthetic Biology standards"'

1. Time to Get Serious about Measurement in Synthetic Biology.

Author

Beal J, Haddock-Angelli T, Farny N, and Rettberg R

Subjects
Base Sequence, DNA analysis, DNA genetics, DNA metabolism, Genetic Engineering instrumentation, Genetic Engineering methods, Humans, Synthetic Biology instrumentation, Synthetic Biology methods, Genetic Engineering standards, Laboratory Proficiency Testing organization & administration, Spectrometry, Fluorescence standards, Synthetic Biology standards
Abstract

For synthetic biology to mature, composition of devices into functional systems must become routine. This requires widespread adoption of comparable and replicable units of measurement. Interlaboratory studies organized through the International Genetically Engineered Machine (iGEM) competition show that fluorescence can be calibrated with simple, low-cost protocols, so fluorescence should no longer be published without units., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Published
2018
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2. DNA assembly standards: Setting the low-level programming code for plant biotechnology.

Author

Vazquez-Vilar M, Orzaez D, and Patron N

Subjects
Biotechnology, CRISPR-Cas Systems, Cloning, Molecular, DNA, Plant genetics, Genetic Engineering standards, Genome, Plant genetics, Plants genetics, Synthetic Biology standards
Abstract

Synthetic Biology is defined as the application of engineering principles to biology. It aims to increase the speed, ease and predictability with which desirable changes and novel traits can be conferred to living cells. The initial steps in this process aim to simplify the encoding of new instructions in DNA by establishing low-level programming languages for biology. Together with advances in the laboratory that allow multiple DNA molecules to be efficiently assembled together into a desired order in a single step, this approach has simplified the design and assembly of multigene constructs and has even facilitated the automated construction of synthetic chromosomes. These advances and technologies are now being applied to plants, for which there are a growing number of software and wetware tools for the design, construction and delivery of DNA molecules and for the engineering of endogenous genes. Here we review the efforts of the past decade that have established synthetic biology workflows and tools for plants and discuss the constraints and bottlenecks of this emerging field., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Published
2018
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3. Realizing the potential of synthetic biology.

Author

Church GM, Elowitz MB, Smolke CD, Voigt CA, and Weiss R

Subjects
Guidelines as Topic, Humans, Synthetic Biology ethics, Synthetic Biology legislation & jurisprudence, Biotechnology, Genetic Engineering, Synthetic Biology standards, Systems Biology
Abstract

Synthetic biology, despite still being in its infancy, is increasingly providing valuable information for applications in the clinic, the biotechnology industry and in basic molecular research. Both its unique potential and the challenges it presents have brought together the expertise of an eclectic group of scientists, from cell biologists to engineers. In this Viewpoint article, five experts discuss their views on the future of synthetic biology, on its main achievements in basic and applied science, and on the bioethical issues that are associated with the design of new biological systems.

Published
2014
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4. DNA tool kit goes live online.

Author

Callaway E

Subjects
Biotechnology standards, Escherichia coli genetics, Gene Regulatory Networks genetics, Genes, Reporter, Genetic Engineering standards, Indicators and Reagents, Promoter Regions, Genetic genetics, Reference Standards, Ribosomes metabolism, Synthetic Biology standards, Biotechnology methods, Gene Expression Regulation genetics, Genetic Engineering methods, Synthetic Biology methods
Published
2013
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5. Synthetic biology. 4th New Phytologist Workshop, Bristol, UK, June 2012.

Author

Osbourn AE, O'Maille PE, Rosser SJ, and Lindsey K

Subjects
Bacteria genetics, Bacteria metabolism, Metabolic Networks and Pathways, Plants genetics, Plants metabolism, Proteins genetics, Proteins metabolism, Synthetic Biology standards, United Kingdom, Yeasts genetics, Yeasts metabolism, Genetic Engineering methods, Synthetic Biology methods
Published
2012
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6. SEVA Linkers: A Versatile and Automatable DNA Backbone Exchange Standard for Synthetic Biology

Author

Morten H. H. Nørholm, Ana Mafalda Cavaleiro, Se Hyeuk Kim, and Maja Rennig

Subjects
0301 basic medicine, DNA Replication, DNA, Bacterial, Protein Conformation, 030106 microbiology, Genetic Vectors, Biomedical Engineering, Computational biology, DNA Fragmentation, Biology, Molecular cloning, Biochemistry, Genetics and Molecular Biology (miscellaneous), law.invention, 03 medical and health sciences, Synthetic biology, chemistry.chemical_compound, Viral Proteins, Plasmid, law, Drug Resistance, Bacterial, Escherichia coli, Synthetic biology standards, Cloning, Molecular, Promoter Regions, Genetic, Base Sequence, Cell factory design, DNA replication, General Medicine, Nicking enzyme, DNA-Directed RNA Polymerases, Small molecule, Standard parts characterization, Anti-Bacterial Agents, 030104 developmental biology, Plasmid backbone exchange, chemistry, Biochemistry, Lac Operon, Recombinant DNA, Synthetic Biology, Genetic Engineering, DNA, Plasmids
Abstract

DNA vectors serve to maintain and select recombinant DNA in cell factories, and as design complexity increases, there is a greater need for well-characterized parts and methods for their assembly. Standards in synthetic biology are top priority, but standardizing molecular cloning contrasts flexibility, and different researchers prefer and master different molecular technologies. Here, we describe a new, highly versatile and automatable standard “SEVA linkers” for vector exchange. SEVA linkers enable backbone swapping with 20 combinations of classical enzymatic restriction/ligation, Gibson isothermal assembly, uracil excision cloning, and a nicking enzyme-based methodology we term SEVA cloning. SEVA cloning is a simplistic one-tube protocol for backbone swapping directly from plasmid stock solutions. We demonstrate the different performance of 30 plasmid backbones for small molecule and protein production and obtain more than 10-fold improvement from a four-gene biosynthetic pathway and 430-fold improvement with a difficult-to-express membrane protein. The standardized linkers and protocols add to the Standard European Vectors Architecture (SEVA) resource and are freely available to the synthetic biology community.

Published
2016

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