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Fragrance rice (Oryza sativa) recognized as an international crop commodity with high price. Betaine- aldehyde-dehydrogenase-2 (Badh2) is known as a key role in the accumulation of fragrant compound in rice, 2-acetyl-1- pyrroline (2AP). Nucleotide deletion of 8bp in exon 7 inactivates Badh2 causing in the increasing level of 2AP. The objective of this study was to determine among 20 Indonesian local rice varieties from Banten, Samarinda and Banyuwangi which genetically potential for producing fragrance rice based on Badh2 gene. Total DNA was isolated from leaves and Badh2 gene was amplified using PCR with specific primers. The evaluation based on amplified Badh2 fragments was carried out by electrophoresis in 1% agarose. Based on this study we suggest that Pandan Ungu from Samarinda and Blambangan A3 from Banyuwangi are the varieties which potential to produce fragrance rice. [ABSTRACT FROM AUTHOR]

Indonesia is a country which has a different altitude within the landscape. Indonesian lowland altitude is fewer than 200 MASL which in turn makes those places prone to flooding. This problem becomes a scourge for rice farmers; therefore, it is urged to take action to maintain food security in Indonesia. Indonesia has many local rice varieties. Based on their morphological and anatomical studies, some of those varieties predicted to have some capability stand over inundations. Submergence-1A (Sub1A) has been reported to be responsible for inundation tolerance. The expression of the Sub1A gene let the rice to survive up to 14 days under inundation stress. The purpose of this study was to look up the presence of the Sub1A gene in Indonesian local rice varieties to find inundation tolerant rice variety. Sub1A gene amplified using PCR with gene-specific primers followed by electrophoresis on 1% agarose. The result of this study has found the Sub1A gene in some rice varieties proved by the presence of specific DNA fragment band. [ABSTRACT FROM AUTHOR]

Abstract. Dapsone Hypersensitive Syndrome (DHS) is the rare case of immunogenic disease that cause physiological damages even death case. However, in Papua Ethnic group this disease was frequently reported among leprosy patients since Dapsone is one of the main drugs in Multi Drugs Therapy standardized by WHO. Previous study in China found that HLA-B 13:01 is strongly associated to DHS in China population. Aim of this study was to detect HLA- B 13:01 gene among DHS leprosy patients in Papua using Sequence Based Typing Methods and qPCR method. This was cross sectional study among 34 leprosy patients that presented with DHS from Papua and West Papua Province. As the negative control we screened 52 leprosy patients that confirmed non-DHS patients. DNA was extracted from 3 mL blood specimens. HLA-B alleles were detected using Sequence Based Typing method by TBG kit and qPCR rapid detection kit using Nalagenetic. The results of HLA-sequence based typing among the 34 leprosy with DHS patients showed that 91,17 % were positively HLA-B:13:01 HLA-B 13:01 carrier consist of 82,35% Heterozygous and 8.82% Homozygous. In negative control 96,15% were non carrier of HLA-B 13:01 gene and 3,85% were heterozygous. Using qPCR procedure, HLA-B 13:01 was positively detected in DHS-leprosy patients and 96.15% negatively detected in Non-DHS leprosy patients but positive in 3.85% of heterozygote non-DHS. The concordances between SBT and qPCR methods were 100%. Conclusion of this study was HLA-B 13:01 gene can be detected in most of DHS leprosy patients in Papua Ethnics Group using SBT as well as qPCR rapid detection kit. [ABSTRACT FROM AUTHOR]

Indonesia home of abundant indigenous and hybrid rice varieties. The phenomenon of phenotipic-plasticity allows varieties or even the same species to exhibit different phenotypic characteristics when planted in different locations. Thus, the identification based on phenotypic characters is needed to be supported by genetic data. The aim of this research was to unveil the relationship between local rice varieties provided from East Java, Banten, and Samarinda based on matK and rbcL genes. Total DNA from leaves were used as template for amplification of both genes. Phylogenetic trees were reconstructed using Neighbour-Joining (NJ) models in Kimura-2 parameters with 1000 bootstraps. The phylogenetic analysis using matK proved that rice varieties from East Java were closely related to rice varieties from Banten rather than to rice varieties from Samarinda. Meanwhile the same study using the rbcL gave inconsistent results [ABSTRACT FROM AUTHOR]

Indonesia is one of the countries with high biodiversity of freshwater fishes. One of the rivers with high freshwater fish diversity is Arut River located in West Kotawaringin, Central Kalimantan. Freshwater fish which is commonly found in the Arut River is striped snakehead. The fish is popular to be consumed by the community due to its delicate and its high nutrient content. However, the research on the genetic characterization of the striped snakehead from Arut River by applying the COI mitochondrial gene has not been conducted. By this reason, the aim of this research was to analyze the genetic characterization of the striped snakehead from Arut River by using the COI mitochondrial gene. The research applied a PCR method with primer F2 and R2. The result exhibited that the five samples of the striped snakehead investigated in this study had 2 haplotypes with 1 variable site without parsimony site. The haplotype diversity and nucleotide diversity were 0.400±0.237 and 0.00068± 0.0004 respectively with a genetic distance of approximately 0.08%. This finding indicates that intrapopulation genetic variation of the fish species is low. In addition, both haplotypes formed on the striped snakehead investigated in this study was different from the striped snakehead from other regions in Indonesia and Southeast Asia, so it can be used as a molecular marker of the striped snakehead from Arut River. [ABSTRACT FROM AUTHOR]