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1. Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli.

Author

Moseley SL, Hardy JW, Hug MI, Echeverria P, and Falkow S

Subjects
Base Sequence, DNA, Bacterial, Diarrhea microbiology, Enterotoxins biosynthesis, Escherichia coli metabolism, Escherichia coli Proteins, Humans, Nucleic Acid Hybridization, Bacterial Toxins, Cloning, Molecular, Enterotoxins genetics, Escherichia coli genetics, Genes, Genes, Bacterial
Abstract

A gene encoding a heat-stable enterotoxin (ST) from an Escherichia coli strain isolated from a human with diarrhea was cloned and characterized by nucleotide sequence analysis. The gene was found to be partially homologous to a previously characterized ST gene from an E. coli strain of bovine origin. Hybridization studies showed that most ST-producing strains of E. coli isolated from humans with diarrhea possess genes highly homologous to either the ST gene from the bovine strain or the ST gene characterized in the present study.

Published
1983
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