1. Response of genes related to iron and porphyrin transport in Porphyromonas gingivalis to blue light.
- Author
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Yuan L, Wang Y, Zong Y, Dong F, Zhang L, Wang G, Dong H, and Wang Y
- Subjects
- Biological Transport genetics, Biological Transport radiation effects, Humans, Gingiva cytology, Fibroblasts cytology, Fibroblasts radiation effects, Hydroxyl Radical metabolism, Heme metabolism, Up-Regulation radiation effects, Homeostasis radiation effects, Down-Regulation radiation effects, Microbial Viability radiation effects, Reactive Oxygen Species metabolism, Aerobiosis, Porphyrins metabolism, Iron metabolism, Porphyromonas gingivalis cytology, Porphyromonas gingivalis genetics, Porphyromonas gingivalis metabolism, Porphyromonas gingivalis radiation effects, Light, Color, Genes, Bacterial radiation effects, Gene Expression Regulation, Bacterial genetics, Gene Expression Regulation, Bacterial radiation effects
- Abstract
Background: Antimicrobial blue light (aBL) kills a variety of bacteria, including Porphyromonas gingivalis. However, little is known about the transcriptomic response of P. gingivalis to aBL therapy. This study was designed to evaluate the selective cytotoxicity of aBL against P. gingivalis over human cells and to further investigate the genetic response of P. gingivalis to aBL at the transcriptome level., Methods: Colony forming unit (CFU) testing, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) were used to investigate the antimicrobial effectiveness of blue light against P. gingivalis. The temperatures of the irradiated targets were measured to prevent overheating. Multiple fluorescent probes were used to quantify reactive oxygen species (ROS) generation after blue-light irradiation. RNA sequencing (RNA-seq) was used to investigate the changes in global gene expression. Following the screening of target genes, real-time quantitative polymerase chain reaction (RT-qPCR) was performed to confirm the regulation of gene expression., Results: A 405 nm aBL at 100 mW/cm
2 significantly killed P. gingivalis within 5 min while sparing human gingival fibroblasts (HGFs). No obvious temperature changes were detected in the irradiated surface under our experimental conditions. RNA-seq showed that the transcription of multiple genes was regulated, and RT-qPCR revealed that the expression levels of the genes RgpA and RgpB, which may promote heme uptake, as well as the genes Ftn and FetB, which are related to iron homeostasis, were significantly upregulated. The expression levels of the FeoB-2 and HmuR genes, which are related to hydroxyl radical scavenging, were significantly downregulated., Conclusions: aBL strengthens the heme uptake and iron export gene pathways while reducing the ROS scavenging pathways in P. gingivalis, thus improving the accumulation of endogenous photosensitizers and enhancing oxidative damage to P. gingivalis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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