1. 2-phenylethylamine catabolism by Escherichia coli K-12: gene organization and expression.
- Author
-
Hanlon SP, Hill TK, Flavell MA, Stringfellow JM, and Cooper RA
- Subjects
- Aldehyde Oxidoreductases metabolism, Amino Acid Sequence, Escherichia coli enzymology, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Genes, Regulator, Molecular Sequence Data, Restriction Mapping, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Species Specificity, Substrate Specificity, Transcription, Genetic, Aldehyde Oxidoreductases genetics, Amine Oxidase (Copper-Containing), Escherichia coli genetics, Genes, Bacterial, Oxidoreductases Acting on CH-NH Group Donors genetics, Phenethylamines metabolism
- Abstract
A gene encoding phenylacetaldehyde dehydrogenase (PAD), the enzyme involved together with a copper-topaquinone-containing amine oxidase in the initial steps of 2-phenylethylamine catabolism, was located at 31.1 min on the Escherichia coli K-12 genetic map. It was immediately adjacent to the gene encoding the amine oxidase but transcribed in the opposite direction. The purified PAD acted almost equally well on phenylacetaldehyde, 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde. It had a subunit size of 54 kDa and its deduced amino acid sequence was approximately 40% identical to various eukaryotic and prokaryotic aldehyde dehydrogenases. A third gene encoding a positive regulatory protein required for expression of the amine oxidase and PAD genes was located next to the PAD gene. A gene previously located in this position was reported to encode a second amine oxidase but this was not confirmed. The nucleotide sequence from 1447 to 1450 kb on the E. coli K-12 physical map has been determined.
- Published
- 1997
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