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Yildirim, Ebru/0000-0002-6289-0729; Kabakci, Ruhi/0000-0001-9131-0933; Elifoglu, Taha Burak/0000-0002-2302-6321 WOS:000560988800020 Di-(2-ethylhexyl) phthalate (DEHP) is widely used as a plasticizer in various products such as PVC-derived plastics, toys, packaging materials, cosmetics, and pharmaceuticals. This study aimed to investigate the effect of DEHP on spontaneous contractions of the feline uterus in vitro. Tubal 1-cm uterine samples prepared from 10, 9, and 12 uteri obtained from adult cats in estrus (n = 5), diestrus (n = 5), and interestrus (n = 5), respectively, after ovariohysterectomy were suspended in an isolated organ bath in aerated Krebs solution at 39 +/- 1 degrees C, and an initial 1 g tension was given. After 1 h equilibration of tissues, the spontaneous contractions were recorded for 10 min as control. The effects of solvent and DEHP (0.001-100 mu M) on contractions were then evaluated in terms of frequency and mean amplitude parameters. It was observed that DEHP had no effect on uterine contractions of cats in interestrus. However, DEHP significantly increased the mean amplitude of uterine contractions during the estrus and diestrus periods at concentrations of 1 mu M and 10 mu M, respectively, depending on the dose (P < 0.05). Decreases in the frequency of the contractions in the estrus and diestrus periods were not statistically significant (P > 0.05). 'this study, carried out for the first time in cats, showed that DEHP has a stimulatory effect on uterine contractions. We concluded that disruption of the uterine contractions, which are essential for physiological reproductive processes such as regular estrous cycles, sperm and zygote transport, implantation and continuation of pregnancy, by DEHP exposure may cause many reproductive problems.

WOS: 000374105800006 The aim of this study was to determine the effects of seasonal variations on the occurrence of total aflatoxin (AFTotal), aflatoxin B1 (AFB1), ochratoxin A (OTA), and total fumonisin (FTotal) in the commercially mixed ruminant feed obtained from feed mills located in Ankara, Kirikkale, Cankiri, Corum, and Kirsehir provinces using the enzyme-linked immunoassay (ELISA) method. To represent the annual production, 22 samples were collected every quarter over 1 year (a total of 88 samples) starting in September 2012. AFTotal and AFB1 were detected in 72 samples (81.81%), OTA in 84 samples (95.45%), and FTotal in 83 samples (94.31%). When seasonal changes were taken into consideration, the highest levels of AFTotal, AFB1, and FTotal (13.57 +/- 8.78 ppb, 8.54 +/- 6.02 ppb, and 0.70 +/- 0.88 ppm, respectively) were found in the summer, while the highest OTA level (57.69 +/- 14.59 ppb) was observed in the spring. The results of high-performance liquid chromatography methods confirmed the results of the ELISA method. Consequently, although most of the feed samples were contaminated with mycotoxins, all the amounts were within the limits allowed for feedstuffs in Turkey. Scientific Research Project Coordination Unit of Kirikkale University (KUBAP) [2012/54] This study was financially supported by the Scientific Research Project Coordination Unit of Kirikkale University (KUBAP No: 2012/54). A part of this study was presented at the 25th Anniversary Annual Scientific Meeting on 4 December 2014, in Geel, Belgium.

WOS: 000357000800002, Veterinary drug residues and their metabolites in food are regularly investigated by local authorities; however their fate in the environment is still unknown. Despite the importance of the aquaculture industry and the widespread use of antibiotics; limited scientific information regarding their residue in natural fish, sediment and sea water are available in TURKEY. The current study; which is the first study in this area, was undertaken to determine the oxytetracycline (OTC), ivermectin (IVM) and emamectin benzoate (EMA) residues from samples of wild fishes (Oblada melanura, Mullus barbatus), sea water and sediment collected in four different months, caught around the fish cages near Salihli Island in Bodrum, Aegean Sea. Samples were analyzed by High Pressure Liquid Chromatography (HPLC) followed by the validation for each matrix. No residues were found to be above the Limit of detection (LOD) levels of the validated methods in the screened samples. In order to understand the possible risk of veterinary antibiotics, especially for low dose accumulation, to the ecosystem for sustainable aquaculture, conduction of more screening analysis with expanded possible matrices would be beneficial., General Directorate of Agricultural Research and Policy (GDAR), Ministry of Food, Agriculture and Livestock [TAGEM 10/AR-GE/20], This study was granted by the General Directorate of Agricultural Research and Policy (GDAR), Ministry of Food, Agriculture and Livestock with the project number: TAGEM 10/AR-GE/20.

The aim of this study was to evaluate the effects of traffic pollution on some haematological parameters, lipid peroxidation and osmotic resistance of erythrocyte in cattle. In the study, totally 38 cattle, living 300 m away (study group, n = 24) and 2.5 km away from the main road (control group, n = 14), were used. Some blood parameters, erythrocyte malondialdehyde (MDA) levels, an indicator of lipid peroxidation, and osmotic resistance were determined in the blood samples taken from the animals. MDA levels of the study group were significantly higher (P, Bu çalışma, trafik kirliliğinin sığırlarda bazı hematolojik parametreler, lipid peroksidasyonu ve eritrosit ozmotik direnci üzerine olan etkilerinin araştırılması amacıyla yapıldı. Çalışmada ana yola 300 m uzaklıkta yaşayan (araştırma grubu, n=24) ve ana yola 2.5 km uzaklıkta yaşayan (kontrol grubu, n=14) toplam 38 sığır kullanıldı. Hayvanlardan alınan kan örneklerinde bazı kan parametreleri, eritrositte lipid peroksidasyonunun bir indikatörü olan malondialdehit (MDA) düzeyi ve eritrosit ozmotik direnci belirlendi. Araştırma grubunda bulunan hayvanlarda MDA düzeyinin daha yüksek olduğu (P

Bu calisma, in vitro kosullarda kadmiyum, bakir ve kursunun follikuler ve luteal evredeki izole inek uterus kasilmalari uzerine olan etkilerinin belirlenmesi amaci ile yapildi. Calismada; Kirikkale mezbahasinda kesilen saglikli Holstein irki ineklerin myometrial seritleri kullanildi. Bu seritler, izole organ banyosuna 2 g on gerimle asildi. Spontan, 2.5 mIU/ml oksitosin ve 10-6 M prostaglandin F2 alfa (PGF2 alfa) ile olusturulan kasilmalar uzerine 10-5 M kadmiyum, 10-4 M bakir ve 10-4 M kursunun etkileri degerlendirildi. Kadmiyum, follikuler evrede oksitosin ve PGF2 alfa’nin, luteal evrede ise spontan, oksitosin ve PGF2 alfa’ nin olusturdugu kasilmalarin tum parametrelerini (maksimum ve ortalama amplitud, frekans degerleri) anlamli olarak azaltti. Bakir; follikuler evrede spontan kasilmalarin ortalama ve maksimum amplitud degerlerini, oksitosin kasilmalarinin tum parametrelerini, PGF2 alfa kasilmalarinin frekans ve maksimum amplitud degerlerini dusurdu. Luteal evrede bakir, spontan kasilmalarin maksimum amplitudunu, oksitosin kasilmalarinin frekans ve maksimum amplitudunu, PGF2 alfa kasilmalarinin ortalama ve maksimumum amplitud degerlerini dusurdu. Kursun, follikuler ve luteal evrede PGF2 alfa ve oksitosin kasilmalarinin frekans degerini azaltirken, luteal evrede spontan kasilmalarin ortalama amplitud degerini artirdi. Sonuc olarak; kadmiyum, bakir ve kursunun inek uterusunun kasilma yanitlarini degistirdigi ve buna bagli olarak fertiliteyi etkileyebilecegi kanisina varildi.

WOS: 000308105200002 PubMed: 22621671 AIM: To determine the effects of Cu and levamisole on concentrations of Cu and Fe in plasma and liver, and the effects of levamisole on lipid peroxidation induced by Cu intoxication in broiler chickens. METHODS: In a 2x4 factorial study, 80 one-day-old Ross PM3 broiler chicks were fed diets for 21 days containing either 8 mg/kg Cu (Low Cu) or 250 mg/kg Cu (High Cu) and were treated with 0 (L0), 4 (L4), 8 (L8) or 16 (L16) mg/kg bodyweight levamisole per day from Day 7 of the study, on three consecutive days in their drinking water. This treatment was repeated three times, at 3-day intervals. On Day 21, blood samples were collected from each bird for analysis of concentrations of Cu, Fe and malondialdehyde, and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase, catalase and glutathione peroxidase (GSH-Px). The birds were killed and liver samples collected for analysis of Cu and Fe. RESULTS: Mean concentrations of Cu and Fe in plasma, and Cu in liver, were increased overall in the High Cu groups compared with the Low Cu groups (p

The aim of this study is to investigate the effect of levamisole on rabbit trachea, the antagonists of levamisole and reveal the relation between trichlorfon and levamisole. The cumulative concentrations of levamisole (10-8 - 10 M) were applied to the tracheal ring of rabbit. Bethanecole, carbachol, acetylcholine, trichlorfon, mecamylamine, atropine and nicotin were also studied with levamisole. Levamisole was without considerable effect alone on rabbit trachea. It was seen that the incubation with levamisole increased the Emax value of acetylcholine (p, Bu araştırmada, levamizolun tavşan trakeası üzerine etkisini belirlemek, levamizolun antagonistlerini saptamak, levamizol ile triklorfon arasındaki ilişkiye açıklık kazandırmak amaçlandı. Levamizol (10-8-10-3 M) birikimli derişimlerde ve 5x10-5M derişimde asetilkolin (Ak), karbakol, betanekol, triklorfon, mekamilamin, nikotin ve atropin ile birlikte değişik terkip ve derişimlerde 82 tavşan trakeası üzerinde çalışıldı. Tavşan trakeası üzerine levamizolun tek başına etkisiz olduğu tespit edildi. Levamizol inkübasyonunun Ak’nın Emax değerini artırdığı (p