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The novel type of microalgae granules (MGs) derived from tiny microalgae cells has received extensive attention due to its great potential for nutrient remediation and resource recovery in wastewater treatment whereas the long start-up time with increased labor expenses remains a bottleneck. In this study, an operation strategy at reduced upflow air velocity (UAV = 0.49 cm/s in R

A reasonable recovery of excess sludge may shift the waste into wealth. Recently an increasing attention has been paid to the recycling of extracellular biopolymers from conventional and advanced biological wastewater treatment systems such as flocculent activated sludge (AS), bacterial aerobic granular sludge (AGS), and algal-bacterial AGS processes. This review provides the first overview of current research developments and future directions in the recovery and utilization of high value-added biopolymers from the three types of sludge. It details the discussion on the recent evolvement of cognition or updated knowledge on functional extracellular biopolymers, as well as a comprehensive summary of the operating conditions and wastewater parameters influencing the yield, quality, and functionality of alginate-like exopolymer (ALE). In addition, recent attempts for potential practical applications of extracellular biopolymers are discussed, suggesting research priorities for overcoming identification challenges and future prospects.

Nitrous oxide (N

Among the common treatment/disposal routes of excessive activated sludge from municipal wastewater treatment plant, dewatering process functions as an essential pre-/post-treatment for volume minimization and transportation facilitation. Since inorganic coagulants have long been criticized for their high dosage and solid residue in sludge cake, there is an urgent need for investigations regarding the potential of applying organic chemicals as the conditioner. In this study, combined use of poly dimethyldiallylammonium chloride (PDMD) and tannic acid (TA) were investigated as an all-organic co-conditioning method for sewage sludge pre-treatment. Results showed that this all-organic conditioning strategy can effectively improve the dewaterability of sewage sludge. The capillary suction time reduced from 128.8 s to 23.1 s, and the filtration resistance reduced from 1.24 × 10

The particular physicochemical and biological properties of nanobubbles (NBs) have prompted many researchers to conduct an in-depth study on their potential application in various fields. This study aims to investigate the effects of nanobubble water (NBW) on the community structure of the gut microbiota in mice. In this study, supplementation with nitrogen NBW (SD-N2 group), hydrogen NBW (SD-H2 group) and deionized water (SD-C group) to a standard diet of mice was performed for five weeks. The composition of fecal microbiota was analyzed by using 16S rRNA gene sequencing. Compared with the SD-C group, the species diversity of the fecal microbiota in mice in NBW groups was significantly increased. At the genus level, supplementation with nitrogen NBW to mice significantly increased the relative abundance of two beneficial genera Clostridium and Coprococcus (mean growth 6.3 times and 9.7 times, respectively), while supplementation with hydrogen NBW significantly decreased the relative abundance of two pathogenic genera Mucispirillum and Helicobacter (mean reduction rate 86% and 60%, respectively). These results demonstrate that supplementation with NBW might optimize the composition of gut microbiota in mice.

Recently, increasing interest has been placed in microalgal-bacterial granular sludge (MBGS) in the journey towards the energy and carbon neutrality of municipal wastewater treatment. Different from aerobic granular sludge, the performance of MBGS is mainly determined by the mutualism and symbiosis between coexisting microalgae and bacteria. It appears from the literature that most of studies on MBGS were conducted at small benchtop scales under controlled conditions with synthetic wastewater. Therefore, this article attempts to look into the major engineering gaps between the knowledge generated from numerous laboratory research works and the large-scale application of MBGS, including massive production of MBGS, type of bioreactor, effect of alternate photo and dark metabolisms on effluent quality, resource recovery from waste MBGS, etc. It is clearly demonstrated that MBGS is still at its infant stage, and more effort is strongly needed to identify the technological bottlenecks of full-scale applications, while providing corresponding engineering solutions.

Calcium ions (Ca

Alkaline-thermal pretreatment was examined for waste activated sludge (WAS) disintegration and subsequent anaerobic digestion (AD). Pretreatment at 60 °C was estimated to provide better economic benefits than higher temperature conditions. The maximum methane yield of 215.6 mL/g COD was achieved when WAS was pretreated at 60 °C and pH 10 for 24 h, which was 46.6% higher than untreated WAS. The pretreatment condition also provided the maximum net savings. The degree of sludge disintegration, considering both loosely bound-extracellular polymeric substance and soluble COD, would be a better indicator to predict anaerobic digestibility than the solubilization rate that considers soluble COD alone. Microbial analysis implied that pretreatment facilitated the growth of hydrolytic bacteria, phyla Bacteroidetes and Firmicutes. In addition, sludge pretreatment enhanced the growth of both acetoclastic and hydrogenotrophic methanogens, genera Methanosaeta and Methanobacterium. The mild AT-PT would be useful to enhance the digestion performance and economic benefit of WAS digestion.

In order to sustainably manage wastewater treatment plants and the environment, enhanced biological phosphorus (P) removal (EBPR) was proposed to achieve P recovery through extracting P-rich liquid (i.e., Phostrip) from the bottom of aerobic granular sludge (AGS)-based sequencing batch reactors (SBRs) under no mixing during the anaerobic phase. Results showed both tested bacterial AGS (BAGS) and algal-bacterial AGS (A-BAGS) systems stably produced low effluent P ( 99%). The collected P-rich liquids (55-83 mg-P/L) from both systems showed great potential for P recovery of about 83.85 ± 0.57 % (BAGS) or 83.99 ± 0.77% (A-BAGS), which were contributed by the influent P (> 95%) and P reserves in granules based on P balance analysis. This study suggests that the AGS-based SBRs coupling the Phostrip holds great potentials for P recovery profit and further reduction in energy consumption.

Air-nanobubble water (NBW) was applied to pre-acclimate anaerobically digested sludge that was then used as the inoculum in the two-stage anaerobic digestion (AD) of high saline (20 g NaCl/L) food waste (FW) to optimize NBW application in the AD of high saline FW. K+ was simultaneously supplemented during the methanogenic stage to resist the inhibition of salt on methanogens. Results showed that after the second pre-acclimation cycle, the inoculum activity was increased 27% in the Air-NBW supplemented reactor in comparison to the deionized water (DW) supplemented one. In the first-stage AD, H2 yield was enhanced by 46% in the Air-NBW pre-acclimated sludge reactor compared with the DW pre-acclimated sludge reactor. Besides, supplementation of K+ in the methanogenic stage could enhance methane production by 17-25% in the DW reactors at initial pH 7.5, 8.0, and 9.0 when compared to the control reactor (using NaOH adjusted initial pH to 7.5), respectively.

A synergistic process was proposed to prepare hydrochar by hydrothermal co-carbonization (HTcoC) of waste distillers grains with sewage sludge, focusing on hydrochar properties and combustion behavior under different mixing ratios. Results show that the co-hydrochar from HTcoC exhibited excellent synergistic characteristics with relatively high synergistic coefficients (0.1-1.2% for hydrochar yield, 4.8-8.0% for higher heating value (HHV), 8.0-12.6% for organic retention, and 2.2-4.0% for carbon retention, respectively), partially evidenced by FTIR data. And the co-hydrochar showed a higher fuel ratio of 0.09-0.13 with the fixed carbon increased to 8.3-10.0 at an remarkably enhanced coalification degree. Moreover, thermal analysis showed that the co-hydrochar exhibited improved combustion efficiency and a more stable flame. As a result, the HTcoC process with 13.0-22.5% increase in biofuel recovery rate and 25.6-47.7% increase in net energy gain may provide an effective approach for the conversion of both biomass wastes into clean biofuel.

Toxic cyanobacterial blooms frequently develop in eutrophic freshwater bodies worldwide. Microcystis species produce microcystins (MCs) as a cyanotoxin. Certain bacteria that harbor the mlr gene cluster, especially mlrA, are capable of degrading MCs. However, MC-degrading bacteria may possess or lack mlr genes (mlr+ and mlr− genotypes, respectively). In this study, we investigated the genotype that predominantly contributes to biodegradation and cyanobacterial predator community structure with change in total MC concentration in an aquatic environment. The 2 genotypes coexisted but mlr+ predominated, as indicated by the negative correlation between mlrA gene copy abundance and total MC concentration. At the highest MC concentrations, predation pressure by Phyllopoda, Copepoda, and Monogononta (rotifers) was reduced; thus, MCs may be toxic to cyanobacterial predators. The results suggest that cooperation between MC-degrading bacteria and predators may reduce Microcystis abundance and MC concentration.

Liquid nitrogen was employed as a low-temperature medium to activate zero-valent iron (ZVI) powder in an attempt to strengthen its enhancement effect on anaerobic digestion (AD) of swine manure (SM). Surprisingly, it was found that both pristine ZVI and liquid nitrogen-pretreated ZVI (LZVI) did not significantly improve the AD performance or change the archaeal community structure. It was hypothesized that ZVI might not be effective at stress-free environment like in these digesters. To confirm this, an additional set of AD experiments were performed at high ammonia stress (about 4000 mg/L), results showed that ZVI and LZVI greatly alleviated ammonia inhibition and increased the CH

This study presents a rapid method on how to speed up aerobic granular sludge (AGS) cultivation and ensure excellent and stable removal performance during bioreactor operation for domestic wastewater treatment. This new strategy consists of start-up the bioreactor using only anaerobic granular sludge (AnGS) as a seed and feeding with crude sewage extracted from a full-scale Extended Aeration Plant. This experiment used a 2.5 L lab-scale sequencing batch reactor (SBR). The bioreactor operated at low dissolved oxygen (DO) concentration controlled at the value of 2.0 mg/L and below. After 60 days of operation, it clearly showed that almost 90% of AnGS seeds turned from black color to brown. The physical characterization analysis showed that the average sizes were unchanged, and the granules remained compact. Also, the SBR operation monitored with brown granules showed stable removal performance. Average removal efficiencies during steady-state cycles at room temperature of COD, ammoniacal nitrogen, and phosphate reached 84%, 92%, and 100%, respectively.

Feammox process is crucial for the global nitrogen cycle and has great potentials for the treatment of low COD/NH

In this study, CuO-CeO2 was synthesized via an easy hydrothermal-calcination method and innovatively applied to peroxymonosulfate (PMS) activation for pollutants degradation under a non-radical oxidation pathway. Singlet oxygen (1O2) was the dominated reactive oxygen species in the CuO-CeO2/PMS system, leading to a dramatical degradation efficiency with Rhodamine B (RhB) as model compounds. The observed rate constant of the CuO-CeO2/PMS system was 7–11 times higher than that of only PMS, CeO2/PMS and CuO/PMS systems. Also, under the reaction conditions of 1.6 mM PMS, 0.4 g/L catalyst and initial pH 7, the degradation efficiencies of RhB, Methylene Blue, Reactive Blue 19 and atrazine were respectively up to 100%, 85.39%, 72.84% and 98.44% in 60 min. X-ray photoelectron microscopy analysis indicated that the electrons transfer between CuO and CeO2 and the formation of oxygen vacancy in CeO2 should be responsible for the enhanced 1O2 production, which involved a new non-radical oxidation pathway for PMS activation by CuO-CeO2 catalyst. Moreover, the combination of CuO and CeO2 increased reusability and stability of catalyst, allowing it remove more than 92% of RhB over a wide pH range (pH = 3–9). This study not only proved that CuO-CeO2 is an efficient and stable PMS activator but also provided a new insight into PMS activation through a non-radical oxidation pathway for organic contaminants removal from wastewater.

To overcome the high separation cost of microalgae, natural microalgae granulation was performed in open sequencing batch reactors (SBRs) by treating synthetic wastewater. After operation for 60 days, easily settled algae granules were obtained with an average size of 0.61 mm, sludge volume index (SVI) of 125 ml/g and settling velocity of 12.2 m/h. More extracellular polymeric substances (EPS) (∼252 mg/g-VSS) were detected to excrete with a higher proteins/polysaccharides (PN/PS) ratio (∼7) for the algae granules on day 60, which are beneficial for granulation. Meanwhile, the algae granules were found to have a higher phosphorus (P) content (33.4 mg-P/g-TSS) with higher P bioavailability (91.8%) when compared to the seed algae (20.4 mg-P/g-TSS). The obtained algae granules possess great potential for P recovery and reuse.

The effects of organic loading rate (OLR) on simultaneous phosphorus (P) and alginate-like exopolymers (ALE) recovery from bacterial aerobic granular sludge (AGS) and algal-bacterial AGS were examined and compared during 70 days' operation. With the increase of OLR (0.6-1.2 g COD/(L·day)), both AGS showed good settleability and granular strength with P bioavailability 92% (Stage III). The moderate increase in OLR had a positive influence on simultaneous recovery of P and ALE. On day 60, the contents of ALE and guluronic acid/guluronic acid (GG) blocks reached the highest in algal-bacterial AGS, about 13.37 and 2.13 mg/g-volatile suspended solids (VSS), respectively. Meanwhile, about daily 0.55 kg of P is estimated to be recovered from the wastewater treatment plant with a treatment capacity of 10,000 m

Algal-bacterial aerobic granular sludge (AGS) was applied for hexavalent chromium (Cr(VI)) biosorption from wastewater and the dynamic distribution and mobility of different metals in granules were systematically examined before and after hydrothermal treatment. The loaded Cr on algal-bacterial AGS was found to mainly localize in microbial cells and mineral particles; little Cr was detected in extracellular polymeric substances (EPS) after 6 h contact, which increased to 5.1% after 24 h biosorption. Along with Cr localization, 9.3-22.8% of Mg and 11.5-26.4% of Ca in algal-bacterial AGS were released from loosely bound EPS, then replenished to maintain their stable proportion in EPS, probably contributing to granular stability. In addition, chemical fractionation showed that the Cr mobility in algal-bacterial AGS, indicated by a low mobility factor of 4.7%, decreased to 1.4% with the co-existence of mineral salts and acetate during biosorption, which was further declined to 0 after hydrothermal treatment.

Zero-valent iron (ZVI) supplementation for improving anaerobic digestion (AD) of ammonia-rich swine manure (initial ammonia-N ∼5000 mg/L) was tested. The addition of 5 g/L ZVI powder apparently accelerated the acidification process to produce more volatile fatty acids (VFAs) and optimized the fermentation type by contributing to a lower system oxidation–reduction potential (ORP) level of −181.7 to −250.0 mV favorable for ethanol-type and butyric-type fermentation during day 14–30, in comparison with that of −164.3 to −216.3 mV in the control group favorable for propionic-type. Overall, ZVI significantly decreased the proportion of propionic acid from 49.8% to 30.9% while increased the proportion of n-butyric acid from 6.8% to 18.7%. Microbial analysis revealed that fast growing and ammonia-tolerant hydrogenotrophic Methanoculleus species were enriched with ZVI, helping achieve a 54.2% higher CH4 yield relative to control. Results from this study demonstrated the potential of ZVI addition to enhance AD of ammonia-rich animal manure.

Resistant pollutants like oil, grease, pharmaceuticals, pesticides, and plastics in wastewater are difficult to be degraded by traditional activated sludge methods. These pollutants are prevalent, posing a great threat to aquatic environments and organisms since they are toxic, resistant to natural biodegradation, and create other serious problems. As a high-efficiency biocatalyst, enzymes are proposed for the treatment of these resistant pollutants. This review focused on the roles and applications of enzymes in wastewater treatment. It discusses the influence of enzyme types and their sources, enzymatic processes in resistant pollutants remediation, identification and ecotoxicity assay of enzymatic transformation products, and typically employed enzymatic wastewater treatment systems. Perspectives on the major challenges and feasible future research directions of enzyme-based wastewater treatment are also proposed.

Nano-bubble water (NBW) has been proven to be effective in promoting organics utilization and CH4 production during anaerobic digestion (AD) process, suggesting its potential in improving the stability of the AD process and thereby alleviating acidic inhibition. In this work, the effect of NBW on digestion stability and CH4 production was investigated to evaluate the ability of NBW on AD recovery from acidic inhibition. Results showed that NBW supplementation increased the total alkalinity (TA) and partial alkalinity (PA), and reduced the ratio of VFA/TA, thus maintained the stability of the AD process. Generation/consumption of VFAs was also enhanced with NBW supplementation under acidic inhibition with pH values of 5.5, 6.0 and 6.5. The cumulative CH4 production was 246–257 mL/g-VS in NBW groups, which was 12.1–17.2% higher than the control. Moreover, with NBW supplementation, the maximum CH4 production rate was raised according to the modeling results.

This study aimed to figure out the main contributors to aerobic phosphorus (P) removal in the algal-bacterial aerobic granular sludge (AGS)-based wastewater treatment system. Kinetics study showed that aerobic P removal was controlled by macropore (contributing to 64-75% P removal) and micropore diffusion, and the different light intensity (0, 4.0, 12.3, and 24.4 klux) didn't exert significant (p 0.05) influence on P removal. On the other hand, the increasing light intensity did promote microalgae metabolism, leading to the elevated wastewater pH (8.0-9.8). The resultant pH increase had a strongly negative relationship (R

Various kinds of vanadium (V) ore mining areas produced serious contamination have been widely recognized, while less relevant research was about the associated health risk and V distribution level for farmland soils around. This study assessed the contamination characteristics and associated human health risk of V in the surface farmland soils near various V ore mining areas. The bioremediation of V contamination by indigenous microbes from them was also evaluated. The farmland soils near stone coal area (Hunan province, China) showed the highest mean concentration of V (543.91 mg/kg), posing high non-carcinogenic risks, with high hazard quotient (HQ) value of 1.29 for children. While, V values of sampled soils near V titanomagnetite, petroleum associated minerals and uvanite areas were lower than that near stone coal area, also with lower HQ values (1.00). Within 60 h, the removal efficiency of V(V) reached 98.4% with farmland soils near uvanite area, suggesting feasibility of V bioremediation via indigenous microbes. Bacterial communities after long-term cultivation (240 d) with V(V) were dominated by native microbes able to tolerate or reduce the toxicity of V(V), such as Ruminococcaceae_incertae_sedis, Trichococcus and Comamonas. This work is helpful for calling attention to V pollution of farmland near various V ore mining areas and formulating effective strategies for V(V) contamination bioremediation.

In this study, high solid anaerobic digestion of pig manure (PM) under nano-bubble water (NBW) addition was investigated with focus on digestion stability, methanogenesis performance and related mechanisms. Volatile fatty acids (VFAs) inhibition occurred when total solids (TS) was about 8% without NBW addition, which was alleviated with improved digestion stability under NBW addition, facilitating the process of high solids anaerobic digestion (HSAD). The cumulative CH4 yield, on the other hand, was 201–230 mL/g-VS in the NBW reactors at TS of 3–6%, about 20.3–25.0% higher than the control reactors. At the same time, with higher water mobility and zeta potential, NBW was found to promote the consumption of soluble proteins/carbohydrates during the above AD process.

This study investigated the synergistic effects of rice straw (RS) and rice bran (RB) addition on methane production and process stability of anaerobic digestion of food waste (FW). Positive synergistic effect (Synergy index (SI) = 1.03–1.24 > 1) was noticed in all the co-digestion reactors. The optimum mixing ratio of FW:RS:RB (volatile solid (VS) basis) was 60:10:30 with the maximum SI (1.24), achieving 27.4% increase in methane yield (235.4 mL/g-VS) and around 5 days shorter of λ (3.7 days) compared to the mono-digestion of FW (184.8 mL/g-VS and 8.2 days). Remarkably high concentration of volatile fatty acids (VFAs) was also accumulated in the mono-digestion of FW, especially propionic acid, which to a great extent caused the methane production to stagnate. Results from this study demonstrate that co-digestion of FW and RS with RB has high potentials for energy recovery from AD of the mixed feedstocks and its stable operation.

Sustainable sewage sludge management is a worldwide issue in wastewater treatment plants (WWTPs). This work developed a new strategy for sewage sludge treatment involving the integration of hydrothermal treatment (HT) with anaerobic co-digestion (AcoD), particularly on the feasibility of mesophilic AcoD of anaerobically digested sludge (DS) hydrolysate and waste activated sludge (WAS). Results show that AcoD of DS hydrolysate from HT 170℃ for 30 min with WAS achieved the highest CH4 production of 205.39 mL CH4/g-VSfed. By adopting the new AD-HT170-AcoD strategy, 61.88 mL CH4/g-tVSfed higher CH4 yield and 22.2% more total solids (TS) reduction were obtained in addition to much better sludge settleability and 7.6% wt. less sludge cake production compared to the conventional mono AD. Although negative energy gain was obtained, the proposed AD-HT170-AcoD strategy is promising, economically feasible, and sustainable when the final sludge disposal of WAS is concerned in the context of whole WWTP.

Nitrite removal is necessary and significant for pickle and meat processing wastewater. In this study, Chlorella sp. L38 is used as an alternative to remove nitrite and reuse it as nitrogen source for potential value-added ingredients production. Based on the typical BG11 medium with and without NaNO3 (which is the conventional nitrogen source), nitrite is additionally provided, and its concentration gradient was set at 0, 50, 100, 150 and 200 μmol/L, respectively. The experimental results showed that the nitrite removal rate could achieve 57.1 μmol/L/d. In addition, the biomass variation, and value-added ingredients (polysaccharides, lipid, and protein) productivity were also measured, and their yield could achieve 4.8 mg/g/d, 3.0 mg/L/d and 5.5 mg/L/d, respectively. It indicated that Chlorella sp. L38 has the potential to be an environmentally friendly approach for nitrite removal of wastewater.

Nanobubble water (NBW) has been proven to efficiently improve methane production from organic solid wastes. However, the increase in reactor volume due to addition of NBW hinders its practical applications. In this study, anaerobically digested sludge was first pre-augmented by N2-NBW and O2-NBW using corn straw as sole substrate for methane production with electron transfer activity being monitored. 20%, 33% and 38% of cellulose and 29%, 35% and 35% of hemicellulose were reduced respectively from the control, N2-NBW and O2-NBW pre-augmented sludge reactors. N2-NBW and O2-NBW pre-augmented sludge reactors achieved methane yields of 127 and 142 mL/g-VS, about 10% and 22% higher than that from the control. Results show that use of NBW pre-augmented anaerobically digested sludge as inoculum can remarkably enhance methane yield from corn straw, providing a novel concept for NBW-based anaerobic digestion system with no increase of reactor volume and construction cost in practice.

Combined effect of zero valent iron (ZVI) and magnetite on semi-dry anaerobic digestion of swine manure was studied. Compared with control, the addition of 5 g/L ZVI, magnetite and their mixture (1:1 wt.) increased the CH4 yield by 17.6%, 22.7% and 21.9%, respectively. The three additives improved CH4 production through altering the metabolism pathways, rather than improving the solid degradation efficiency. ZVI promoted interspecies hydrogen transfer (IHT) by enriching H2-comsuming Methanolinea and acetate-oxidizing bacteria (Sedimentibacter and Clostridium). Magnetite enriched dissimilatory iron reduction bacteria (Acinetobacter) to accelerate organic hydrolysis and established direct interspecies electron transfer (DIET) by enriching Methanothrix and Methanospirillum. Key microorganisms relative to IHT (Clostridium) and DIET (Methanothrix and Methanospirillum) were simultaneously enriched with ZVI+magnetite, but they only showed an additive effect on methanogenesis, the lack of synergetic effect was attributable to the possible trade-off between IHT and DIET, or the little improvement effect of additives on substrate biodegradability.

Biogas upgrading is an essential process for efficient and safe utilization of biogas produced from anaerobic digestion (AD), a cost-effective and environmentally friendly technology for bioenergy recovery from organic wastes. Biogas recirculation in AD reactors has been recently reported as a cost-effective and promising method to enhance methane content in biogas. This review aimed to summarize the state-of-art of biogas recirculation-based AD systems to better understand the possible mechanisms and main factors relating to in-situ biogas upgrading. It shows that biogas recirculation in the AD reactor can not only enhance methane content via both physicochemical and biological effects, but also help establish a robust AD system with high buffering capacity for highly efficient treatment of various organic wastes. More research works are demanding for a better understanding of the mechanisms and the optimization of the whole AD system, targeting its further development for high-calorie bioenergy production.

Anaerobic digestion has been recognized as promising technology for bioenergy production, while the bottlenecks including long start up times, low methane contents, and susceptibility toward environmental change attenuate the process benefits. Integrating microbials electrolysis cell (MEC) with anaerobic digestion (AD) has been recognized as a promising strategy for alleviate the performance bottleneck. This review summarized and updated the current researches that utilize MEC-AD for enhanced methane production from biomass. The integrated AD-MEC was first elucidated, followed by illustrations on strategies for process performance enhancements, parameters effects, and the associated applications. Finally, the challenges and prospects were outlined in this work.

This work investigated the potential of vanadium (V) (V(V)) bioreduction by using soils sampled from four main kinds of vanadium ore mining areas, i.e. vanadium titanomagnetite, stone coal, petroleum associated minerals and uvanite as inocula. During a typical operation cycle of 60 h, the soils from vanadium titanomagnetite area and petroleum associated minerals area exhibited higher V(V) removal efficiencies, about 92.0 ± 2.0% and 91.0 ± 1.9% in comparison to 87.1 ± 1.9% and 69.0 ± 1.1% for the soils from uvanite and stone coal areas, respectively. Results from high-throughput 16 S rRNA gene pyrosequencing analysis reflect the accumulation of Bryobacter and Acidobacteriaceae with capabilities of V(V) reduction, accompanied with other functional species. This study is helpful to search new functional species for V(V) reduction and to develop in situ bioremediations of V(V) polluted groundwater.

A lab-scale intermittently aerated sequencing batch reactor (IASBR) was applied to treat anaerobically digested swine wastewater (ADSW) to explore the removal characteristics of veterinary antibiotics. The removal rates of 11 veterinary antibiotics in the reactor were investigated under different chemical organic demand (COD) volumetric loadings, solid retention times (SRT) and ratios of COD to total nitrogen (TN) or COD/TN. Both sludge sorption and biodegradation were found to be the major contributors to the removal of veterinary antibiotics. Mass balance analysis revealed that greater than 60% of antibiotics in the influent were biodegraded in the IASBR, whereas averagely 24% were adsorbed by sludge under the condition that sludge sorption gradually reached its equilibrium. Results showed that the removal of antibiotics was greatly influenced by chemical oxygen demand (COD) volumetric loadings, which could achieve up to 85.1% ± 1.4% at 0.17 ± 0.041 kg COD/m-3/day, while dropped to 75.9% ± 1.3% and 49.3% ± 12.1% when COD volumetric loading increased to 0.65 ± 0.032 and 1.07 ± 0.073 kg COD/m-3/day, respectively. Tetracyclines, the dominant antibiotics in ADSW, were removed by 87.9% in total at the lowest COD loading, of which 30.4% were contributed by sludge sorption and 57.5% by biodegradation, respectively. In contrast, sulfonamides were removed about 96.2%, almost by biodegradation. Long SRT seemed to have little obvious impact on antibiotics removal, while a shorter SRT of 30–40 day could reduce the accumulated amount of antibiotics and the balanced antibiotics sorption capacity of sludge. Influent COD/TN ratio was found not a key impact factor for veterinary antibiotics removal in this work.

Anaerobic digestion of decabromodiphenyl ether was carried out and compared in two continuously stirred anaerobic bioreactors for 210 days under thermophilic and mesophilic conditions. Results show that the degradation of decabromodiphenyl ether followed the first-order reaction kinetics, which exhibited a higher removal rate in the thermophilic reactor when compared to the mesophilic one, reaching its maximum of 1.1 μg·day−1. The anaerobic digestion of decabromodiphenyl ether was found to involve the replacement of bromines from polybrominated diphenyl ether by hydrogen atoms, gradually forming nona-, octa- and hepta-brominated diphenyl ether, respectively. Under the thermophilic condition, the reactors were dominated by Bacillus sp. and Methanosarcina sp. with high bioactivity and high concentrations of debromination microorganisms.

Anaerobic digestion (AD) of ammonia-rich swine manure (SM) with nano-bubble water (NBW) supplementation was studied in this work with the expectation of ammonia inhibition alleviation, buffering capacity promotion, and methane production enhancement. Results indicated that cumulative methane yield was elevated by 12.3-38.7% in NBW groups. Besides, the reduced methane production rate and elongated lag phase under ammonia inhibition were increased and shortened by NBW supplementation, respectively. The rapid increase of total alkalinity (TA) and partial alkalinity (PA) could be observed with NBW supplementation, as well as the rapid decline of VFA/TA, thus improved buffering capacity and alleviated ammonia inhibition. Moreover, higher level of extracellular hydrolases and coenzyme F420 could be detected in NBW groups. In conclusion, NBW with higher mobility and zeta potential (absolute value) could be a promising strategy for the alleviation of ammonia suppression during the AD of SM.

In order to dispose excess sewage sludge and recycle its nitrogen and phosphorus resources, hydrothermal pretreatment coupling with MgNH4PO3·6H2O (MAP) crystallization was investigated. Hydrothermal pretreatment at different temperature and residence time were performed on anaerobically digested sludge to maximize phosphate and ammonium release and their recovery from the resultant hydrolysate. Results showed that hydrothermal pretreatment at 200°C for 30min favored phosphate and ammonium release achieving phosphate and ammonium concentrations at 579.98mg-P/L and 456.85mg-N/L, respectively. During the subsequent MAP precipitation, the optimum condition for N and P recovery was determined at pH 9 and Mg/P molar ratio of 1, realizing P and N recovery of 91.60% and 54.88% respectively from the hydrolysate in addition to MAP purity of 84.24%. The produced MAP was confirmed by FTIR and XRD analysis, which fulfills the related standards and can be used as fertilizer with high promise.

Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence. Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps. The recovery rates based on MPS used were 26.2%, 29.1% and 18.7% for MP-1, MP-3 and MP-4 respectively. The monosaccharide composition, ultraviolet spectrum, infrared spectrum and NMR of the three fractions were analyzed. Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated. The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL. The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase. These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.

In this study, a proof-of-concept of bioleaching and recovery of copper (Cu) from E-wastes assisted by direct current (DC) electric field was proved in a bioelectrical reactor. Results showed that 40 mA electric current application could not only significantly shorten the leaching time of Cu from 5 (control) to 3 days with 100% leaching efficiency, but also recover about 97% leached Cu ions within 4 days. DC electric field improved the activity and growth of iron oxidizing bacteria and facilitated Fe2+ oxidation, which resulted in effective leaching of Cu from printed circuit boards (PCBs). The functional Acidithiobacillus was selectively enriched by DC electric field for enhancing the efficiency of bioleaching. At the same time, the leached Cu ions were rapidly electrodeposited on the cathode, achieving the recovery of Cu. Hence, this work provided a novel strategy for metals bioleaching and recovery from E-wastes.

In this study, CH4 production from anaerobic digestion (AD) of refractory cellulose was investigated at a high loading of 3.5 (VScellulose/VSinoculum) under nanobubble water (NBW) addition. A longer proton spin-spin relaxation time (2611–2906 ms) of NBW during 35 days’ storage reflected its high mobility and diffusion of water molecules. Higher volatile fatty acids were yielded at the hydrolysis-acidification stage under NBW addition. Methanogenesis tests showed that Air-NBW and CO2-NBW supplementation accelerated the utilization of crystalline cellulose, achieving methane yields of 264 and 246 mL CH4/g-VSreduced, increasing by 18% and 10% compared to deionized water addition (the control), respectively. In addition, under NBW addition the cellulose crystallinity reduction was enhanced by 14–20% with microbial community being enriched with hydrolytic and methanogenic bacteria. Results from this work suggest that NBW environment with no chemical addition and relatively low energy consumption is advantageous for enhanced AD process of cellulosic biomass.