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To explore the mechanism of extraction and enrichment of three nitrophenol isomers by charge-transfer supramolecular synergistic three-phase microextraction system, a charge transfer supramolecular-mediated hollow fiber liquid-phase microextraction (CTSM-HF-LPME) combined with high-performance liquid chromatography-ultraviolet detector (HPLC-UV) method was established for the determination of real environmental water samples. In this study, the three nitrophenols (NPs) formed charge-transfer supramolecules with electron-rich hollow fibers, which promoted the transport of NPs in the three-phase extraction system and greatly increased the EFs of NPs. The relationships between the EFs of NPs and their solubility, pK

Herzog and de Meuron were 2001 Pulitzer prize winners. Their buildings are famous because of the unique and amazing epidermis. In their representative works, there are two major ways to create and form epidermis. The first one is learning from the surrounding context and environment, and the second is thinking about the features of architectures. This paper, through the investigation of six of their major works: Dominus Winery in California of the US(1998), De Young Museum in San Francisco (2004), the library of Brandenburg Industrial University in Germany, the Tokyo Prada flagship store in Japan, Munich Allianz Stadium in Germany, and Laban Centre for Contemporary Dance in London, summarizes the main methods of H&dM.Architecture surface processing is becoming more and more concerned by the construction industry and the viewers. H&dMs methods of design provide our use for reference. Let us look forward to their next new work.

GEO-groove simulation experiment is applied to imitate the Taiyuan soil subsidence in pipeline base and side by soil loss, which eventually leads the pipeline to be deformed and destroyed. The pre-damage standard is set up though analyzing test statistics and drawing it’s sedimentation and damage curve. The damage standard of sewage line is achieved through setting up a very pilot model to the test and analyzing it’s bearing force characteristics, and according to the gotten sedimentation curve of the test, damage characteristics and mechanism of sewage line is analyzed , thus will give the designing and application of pipeline of testing and theory basement, so as to take measures to protect pipeline in practical situations, establish early warning system to pipeline, and guarantee it’s regular service.

Currently it is still a lack of reliable evidences on the evaluation of the bearing capacity of foundation in highway construction of Shanxi Province. Mainly reason is that the method widely used to determine in bearing capacity of loess foundation in building structures is not completely suitable to length and wide range of highway subgrade project. Because Shanxi loess region terrain is very complex, the capacity of Q3 loess-like silt in Yuncheng of Shanxi is collected and analyzed, based on statistics of a large number of bearing capacity from site loading tect. A linear regression relationship is established between loess foundation bearing capacities and water content, liquid limit, void ratio. Another linear regression relationship is established between saturation and liquid limit void ratio . Meanwhile, it compares these two sets of linear regression relationship with the measured bearing capacity and the carrying capacity by specification. Applied result show that the relative errors of the bearing capacities computed by the formulas are less than 10%, compared with other standard methods to determine the loess bearing capacity. It is significantly improvement for meeting the requirements of the highway construction.

Through the model test in soil slot,the process of deformation and destruction of soft pipeline caused by subsoil settlement is simulated and observed in lab. Based on the experimental data, the curve as the internal force of pipeline is first drawn out and discussed, in turn the characteristic of stress distribution and destruction rule of pipeline are analyzed and summarized, which may be referred for designer to take appropriate measures. The experimental results show that the internal force and deflection of the pipeline increase with the depth and width of the hole below the pipeline. When the hole depth (h)/ pipeline diameter (D) =2.5, internal force and deflection changes only associate with the width of the hole (b). And when b/D =6, the pipeline can not work normally and even be destroyed. The experimental conclusion can provide the basis for the security of underground pipeline.

The subject of Foundation Excavation near the buried pipeline line. Compared with each other using a scale model of the buried pipeline foundation excavation factor of the vertical displacement, and connected with the buried pipeline displacement of both ends of the fixed elastic beam theory; analysis of the factors affecting the displacement of buried pipelines, the good agreement of the theoretical results with the scale model test value.

A series of compression tests were conducted on the samples taken from compacted soil site in Lulling, in order to investigate the compressibility of compacted loess in different water content and different compaction energy. Through statistics analysis of the testing data, the stress-strain curve of compacted loess could be expressed with Hyperbolic form. And the relation between initial tangent modulus and water content change coefficient was founded by introducing the water content change coefficient. The result shows that at the same compaction energy, the initial tangent modulus decreases and the ultimate strain increases as water content increases; while at the same water content change coefficient, the initial tangent modulus increases and the ultimate strain decreases as compaction effort increases.

This paper studies the microstructure of 16 group samples from three different sites of North-west region in P. R. China. Pictures of microstructure were processed with imaging processing program to acquire the geometer parameter and fractal dimension of pores. The range of pores’ fractal dimension is between 1.816~1.936. The pore distribution function of unsaturated soil is established based on fractal geometry theory. The pore water distribution of loess under natural moisture is analyzed. The relationships of collapsibility coefficient with fractal dimension and porosity of unsaturated pores are analyzed with the method of regression analysis. A larger fractal dimension represents a more complex pore-structure. The collapsibility coefficient of loess increases with the increase of fractal dimension and porosity of unsaturated pores.

The degree of compaction is usually used as the compaction quality and control indicator of backfill in practical project. However, as the degree of compaction is affected by various internal and external factors, its accuracy is difficult to guarantee. In this paper, compacted loess samples were prepared under different compaction energies by normal compaction method. The curves of compression coefficient and dry density, the compression coefficient and porosity of compaction loess samples under different compaction energy are analyzed while the water content is constant. The air porosities of compaction loess samples under different compaction energy and water content are calculated and summed up. The air porosity of compaction loess samples under different compaction energy is more stable than the degree of compaction when the water content is exactly equal to the optimum water content. The rationality of using air porosity as the loess compaction quality control indicator is discussed. It is proposed using air porosity as additional indicator of compaction quality control on the condition of the loess compacted dry density meeting the requirements. The air porosity less than 6.5% is suggested as the additional quality control indictor for region backfill compaction.

Contamination can change the physical properties of soil, reduce the strength of cemented soil foundation, and then induce the instability or disruption of buildings. This study explored the relationships among the age, electrical resistivity, unconfined compression strength, contamination of municipal sewage concentration. Firstly, we mixed the silty clay with municipal sewage of five different concentrations to form the contaminated soil. Then, mixed the contaminated soil with ordinary portland cement to form the cemented soil blocks. Finally, measured the unconfined compression strength and electrical resistivity of these blocks. The results show that the unconfined compression strength and electrical resistivity increase linearly with the increase of the logarithm of age, and decrease exponentially with the increase of municipal sewage concentration. The unconfined compression strength of cemented soil has a linear relationship with its resistivity. The municipal sewage concentration and age can be use to predict the unconfined compression strength of cemented soil.The predicted values correlate well with the experimental values.

With the Drucker-Prager yield criterion, the ANSYS software is used to analyze the long-short-pile composite foundation by using three-dimensional nonlinear finite element method. The influences of load on axial stress and maximal axial stress of head of pile with cushion or not have been investigated by simulating composite foundation through nine long piles and sixteen short piles. The result indicates the cushion can reduce the stress concentration in head of pile effectively with increase of load. This conclusion has a certain value for engineering.

A novel hollow fiber cell fishing with high performance liquid chromatography (HFCF-HPLC) was extended and used to screen flavonoid and anthraquinone active compound groups simultaneously from traditional Chinese medicines (TCMs). In this study, three cells (MCF-7, SGC7901, and MADB-106) were seeded on the inner wall of the hollow fiber employed to screen bioactive components from TCM water decoction. The variables influencing HFCFHPLC, such as cell seeding time, screening stirring rate and time, and active compound concentration, were investigated and optimized. The surface property of the hollow fiber seeded with cells, the cell survival rate under different conditions, the nonspecific binding between active centers in the fiber and the target compounds, and the repeatability and recovery of HFCF-HPLC were analyzed and validated. Certain structures of the compounds fished by HFCF-HPLC were identified after comparing the retention times of the reference substances. To verify preliminarily the binding site between the bioactive components and cells, we separated the cell membrane and cell organelle from live MCF-7 cells. We then employed the cell membrane, cell organelle, and the whole cells to screen simultaneously the active compounds. The cell fishing factor of the active compound was calculated and discussed as the index of cell-drug binding ability in HFCFHPLC. Tamoxifen as a positive control and indomethacin as a negative control were screened by HFCF-HPLC to verify the method. The results indicate that HFCF-HPLC is an effective and reliable method for the screening and analysis of bioactive components. Moreover, this method can be applied to predict bioactive candidates in TCMs.

The carcinogenic or tumourigenic testing of seven animal kidney cell lines (F-81, CRFK, MDCK, Vero, Vero-2 cell line, MA-104 and BHK-21) established in China, were carried out in more than 700 nude mice for colony formation in soft agar and for agglutination under different density of plant lectins. Tests showed that there were correlation between cell line chromosome number variations and anchorage independence in soft agar, agglutinability under lectins and tumour-forming ability in nude mice. Since testing in vitro was more economical, simpler and faster and thus thought to be more reliable, we recommend measuring agglutinability, followed by anchorage independence or analysis of karyotype as the initial means for monitoring tumourigenicity of animal cell lines in nude mice.

Hollow fibre cell fishing with high performance liquid chromatography (HFCF-HPLC) is a newly developed method used to screen and fish bioactive compounds in traditional Chinese medicines (TCMs). In the study, colorectal cancer cell HCT116 was first seeded in a hollow fibre and used for screening and fishing active compounds from TCMs. The surface properties of the hollow fibre seeded with HCT116 cells, the non-specific binding between active centres in the fibre and the target compounds, the cell survival rate under different conditions before and after screening, the repeatability and recovery of HFCF-HPLC were investigated in detail. The cell fishing factor of active compound was defined in HFCF-HPLC. We employed HFCF-HPLC to screen and fish anthraquinones active compounds group from extracts of Polygonum cuspidatum, Cecropia obtusifolia L. and Polygoni multiflori radix praeparata. Some of the anthraquinones structures screened from TCMs were identified by comparing to the retention time of the reference substances confirmed by mass spectrometry. The ability of permeable membrane of anthraquinones screened by HFCF-HPLC was further described. Indomethacin was used as the positive control substance. Results demonstrated that HFCF-HPLC is an effective, stable and reliable method to screen and analyse bioactive compounds. Other bioactive compounds from TCMs could also be screened.