6 results on '"Wei-Wei Du"'
Search Results
2. Exposure parameters and health risk of Cryptosporidium and Giardia in the recreational water activities for urban residents in China
- Author
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Chong-Miao Zhang, Wei-Wei Du, Pengcheng Xu, and Xiaochang C. Wang
- Subjects
Infection risk ,Health risk assessment ,biology ,Health, Toxicology and Mutagenesis ,Giardia ,Cryptosporidium ,General Medicine ,biology.organism_classification ,Pollution ,Geography ,Environmental health ,Environmental Chemistry ,Health risk ,Risk assessment ,China ,Recreation - Abstract
Knowledge gaps in the exposure parameters for recreational water activities make quantitative risk assessment related to water recreation difficult. Therefore, the annual exposure frequency and single exposure duration for the recreational water activities of residents from ten cities in the North and South of China were investigated. Questionnaire interviews were carried on recreational water activities comprising swimming (SW), boating (BA), playing in interactive fountains (PF), and watching fountains (WF). Quantitative microbial risk assessment for the exposure of urban residents to Cryptosporidium and Giardia was also performed. For the four recreational water activities, the participation rates of urban residents in SW and WF were higher than the others. For SW and BA, the mean annual exposure frequency and single exposure duration for males were significantly higher than those for females. PF and WF showed the opposite. The annual exposure frequency for above 35-year-old residents was higher than that for young residents (18–35 years). However, the single exposure duration for young residents was highest in SW, BA, and PF. The mean annual exposure frequency and single exposure duration for North China residents were higher than those for South China residents in all recreational water activities, except for SW. Overall, the annual exposure frequency and single exposure duration in recreational water activities for all urban residents followed a lognormal distribution. In the four recreational water activities, the total annual infection risk of male exposure to Cryptosporidium was 1.0 × 10-2, with the confidence intervals between 95 and 5% of [4.3 × 10-4, 3.7 × 10-2], whereas that for females was 6.8 × 10-3 and [4.2 × 10-4, 2.4 × 10-2]. Also, the annual infection risk of males to Giardia was 8.8 × 10-3 and [5.1×10-4, 3.2×10-2], and that of females was 5.3 × 10-3 and [4.0 × 10-4, 1.8 × 10-2]. These results demonstrated that SW and PF made the highest contribution to the total annual infection risk. Sensitivity analysis highlighted that the characterization of exposure parameters plays a critical role in health risk assessment, which may provide a scientific basis for recreational water quality standards formulation.
- Published
- 2021
3. Inhibition of neuroblastoma proliferation by PF-3758309, a small-molecule inhibitor that targets p21-activated kinase 4
- Author
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Xiaolu Li, Li-Xiao Xu, Xiaolan Chen, Wei-Wei Du, Guang-Hui Qian, Chun Yang, Yi Wu, Jian Wang, Shaoyan Hu, He Zhao, Jian Pan, Fang Fang, Jun Lu, Xin Ding, Junli Ren, Zhi-Heng Li, Mei Li, Yunyun Xu, and Yan-Fang Tao
- Subjects
Adult ,Male ,0301 basic medicine ,Cancer Research ,Cell cycle checkpoint ,Cell Survival ,MAP Kinase Signaling System ,Cell ,Biology ,neuroblastoma ,03 medical and health sciences ,Cell Line, Tumor ,Neuroblastoma ,medicine ,Humans ,Pyrroles ,Protein Kinase Inhibitors ,Cell Proliferation ,Neoplasm Staging ,Oncogene ,Kinase ,Cell Cycle ,apoptosis ,Articles ,General Medicine ,Cell cycle ,medicine.disease ,Up-Regulation ,Cell biology ,Gene Expression Regulation, Neoplastic ,PF-3758309 ,growth arrest ,030104 developmental biology ,medicine.anatomical_structure ,p21-Activated Kinases ,Oncology ,PAK4 ,Apoptosis ,Cancer cell ,Cancer research ,Pyrazoles ,Female - Abstract
Neuroblastoma is the most common extracranial solid childhood tumor. Despite the availability of advanced multimodal therapy, high-risk patients still have low survival rates. p21-activated kinase 4 (PAK4) has been shown to regulate many cellular processes in cancer cells, including migration, polarization and proliferation. However, the role of PAK4 in neuroblastoma remains unclear. In the present study, we demonstrated that PAK4 was overexpressed in neuroblastoma tissues and was correlated with tumor malignance and prognosis. To investigate the function of PAK4 in neuroblastoma, we used a small-molecule inhibitor that targets PAK4, that is, PF-3758309. Our results showed that PF-3758309 significantly induced cell cycle arrest at the G1 phase and apoptosis in neuroblastoma cell lines. Meanwhile, the inhibition of PAK4 by PF-3758309 increased the expression of CDKN1A, BAD and BAK1 and decreased the expression of Bcl-2 and Bax. In addition, we screened the target genes of PAK4 by PCR array and found that 23 genes were upregulated (including TP53I3, TBX3, EEF1A2, CDKN1A, IFNB1 and MAPK8IP2) and 20 genes were downregulated (including TNFSF8, Bcl2-A1, Bcl2L1, SOCS3, BIRC3 and NFKB1) after PAK4 inhibition by PF-3758309. Moreover, PAK4 was found to regulate the cell cycle and apoptosis via the ERK signaling pathway. In conclusion, the present study demonstrated, for the first time, the expression and function of PAK4 in neuroblastomas and the inhibitory effect of PF-3758309, which deserves further investigation as an alternative strategy for neuroblastoma treatment.
- Published
- 2017
4. Inhibiting PLK1 induces autophagy of acute myeloid leukemia cells via mammalian target of rapamycin pathway dephosphorylation
- Author
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Yanhong Li, Guang-Hui Qian, Jian Wang, Shaoyan Hu, Xing Feng, Wei-Qi He, Li-Xiao Xu, Xie Yi, Jian Pan, Mei Li, Yan-Fang Tao, Zhi-Heng Li, Xiaolu Li, Wei-Wei Du, Yi-Ping Li, Gang Li, Fang Fang, Jun Lu, Junli Ren, and Yi Wu
- Subjects
Male ,0301 basic medicine ,Cancer Research ,Cell Cycle Proteins ,Mice ,0302 clinical medicine ,hemic and lymphatic diseases ,Tumor Cells, Cultured ,Phosphorylation ,RNA, Small Interfering ,Child ,Reverse Transcriptase Polymerase Chain Reaction ,TOR Serine-Threonine Kinases ,RO3280 ,Myeloid leukemia ,Articles ,General Medicine ,Cell cycle ,Prognosis ,Cell biology ,Survival Rate ,Leukemia, Myeloid, Acute ,mTOR phosphorylation ,Oncology ,030220 oncology & carcinogenesis ,polo-like kinase 1 ,Female ,Signal Transduction ,autophagy ,Programmed cell death ,Blotting, Western ,Protein Serine-Threonine Kinases ,Biology ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Proto-Oncogene Proteins ,Biomarkers, Tumor ,Animals ,Humans ,RNA, Messenger ,Protein kinase A ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Neoplasm Staging ,BI2536 ,Autophagy ,pediatric acute myeloid leukemia ,Xenograft Model Antitumor Assays ,030104 developmental biology ,Cancer research ,K562 cells - Abstract
Decreased autophagy is accompanied by the development of a myeloproliferative state or acute myeloid leukemia (AML). AML cells are often sensitive to autophagy‑inducing stimuli, prompting the idea that targeting autophagy can be useful in AML cytotoxic therapy. AML NB4 cells overexpressing microtubule-associated protein 1 light chain 3-green fluorescent protein were screened with 69 inhibitors to analyze autophagy activity. AML cells were treated with the polo-like kinase 1 (PLK1) inhibitors RO3280 and BI2536 before autophagy analysis. Cleaved LC3 (LC3-II) and the phosphorylation of mammalian target of rapamycin (mTOR), adenosine monophosphate-activated protein kinase, and Unc-51-like kinase 1 during autophagy was detected with western blotting. Autophagosomes were detected using transmission electron microscopy. Several inhibitors had promising autophagy inducer effects: BI2536, MLN0905, SK1-I, SBE13 HCL and RO3280. Moreover, these inhibitors all targeted PLK1. Autophagy activity was increased in the NB4 cells treated with RO3280 and BI2536. Inhibition of PLK1 expression in NB4, K562 and HL-60 leukemia cells with RNA interference increased LC3-II and autophagy activity. The phosphorylation of mTOR was reduced significantly in NB4 cells treated with RO3280 and BI2536, and was also reduced significantly when PLK1 expression was downregulated in the NB4, K562 and HL-60 cells. We demonstrate that PLK1 inhibition induces AML cell autophagy and that it results in mTOR dephosphorylation. These results may provide new insights into the molecular mechanism of PLK1 in regulating autophagy.
- Published
- 2017
5. Contribution to Study of the Sintering Mechanism
- Author
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Wei Wei Du and Lyes Douadji
- Subjects
Surface tension ,chemistry.chemical_compound ,Materials science ,chemistry ,Powder metallurgy ,Scientific method ,Heat transfer ,Flow (psychology) ,Metallurgy ,Sintering ,Particle ,General Medicine ,Polyvinylidene fluoride - Abstract
In all process based in powder and in particular in the rotational moulding process, the major physical phenomenon during the flow of the powders is the sintering and grains growth. The sintering is the formation of only one particle starting from two or many particles under the effect of the temperature and the forces of surface tension. We interest in this study in particular at the mechanism of Polyvinylidene fluoride (PVDF) sintering. The results of this study can enable us to maximize our knowledge about sintering mechanism and enable us to determine parameters such as the speed of sintering for the optimization of the process.
- Published
- 2014
6. A Study on Migration of Contamination from Paper Cups to Ethanol by HPLC
- Author
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Ya Ning Xu, Jian Qing Wang, Qing Qing Yue, Wei Wei Du, and Ying Wang
- Subjects
Pollutant ,chemistry.chemical_compound ,Ethanol ,Chromatography ,Waste management ,Chemistry ,Dibutyl phthalate ,Benzophenone ,Alcohol ,General Medicine ,Contamination ,High-performance liquid chromatography - Abstract
The main objective was to develop a technique by high performance liquid chromatography to study migration of contamination from packaging material of three samples of paper cups with different properties. Dibutyl phthalate (DBP) and benzophenone (BP), which are the most commonly used in paper packaging material, are regarded as stimulant of contamination, alcohol is selected as food stimulant.The obtained data indicated that migration has a relationship with the type of contamination,concentration of food stimulant.Analysed by1stOptsoftware with experimental result, the rule of migration of DBP and BP can be studied.
- Published
- 2013
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