85 results on '"Ting Ren"'
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2. Diffusion simulation and risk assessment model establishment of chlorine gas leakage based on terrain conditions
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Xiao-ting Ren, Xiao-ling Ma, Jiang-zheng Liu, Rui Liu, Chen-qian Zhao, Hao Wu, Zhao Wang, Chun-xu Hai, and Xiao-di Zhang
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Health, Toxicology and Mutagenesis ,Environmental Chemistry ,General Medicine ,Pollution - Published
- 2023
3. The outcomes of margin status after sleeve lobectomy for patients of non–small cell lung cancer
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Jianghao Ren, Mingyang Zhu, Yuanyuan Xu, Ruijun Liu, Ting Ren, Zhiyi Guo, Jiangbin Ren, Kan Wang, and Qiang Tan
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Pulmonary and Respiratory Medicine ,China ,Lung Neoplasms ,Oncology ,Carcinoma, Non-Small-Cell Lung ,Humans ,General Medicine ,Neoplasm Recurrence, Local ,Pneumonectomy ,Neoplasm Staging ,Retrospective Studies - Abstract
Sleeve lobectomy is recognized as an alternative surgical operation to pneumonectomy because it preserves the most pulmonary function and has a considerable prognosis. In this study, we aimed to investigate the implications of residual status for patients after sleeve lobectomy.In this retrospective cohort study, we summarized 58 242 patients who underwent surgeries from 2015 to 2018 in Shanghai Chest Hospital and found 456 eligible patients meeting the criteria. The status of R2 was excluded. The outcomes were overall survival (OS) and recurrence-free survival (RFS). We performed a subgroup analysis to further our investigation.After the propensity score match, the baseline characteristic was balanced between two groups. The survival analysis showed no significant difference of overall survival and recurrence-free survival between R0 and R1 groups (OS: p = 0.053; RFS: p = 0.14). In the multivariate Cox analysis, we found that the margin status was not a dependent risk factor to RFS (p = 0.119) and OS (p = 0.093). In the patients of R1, N stage and age were closely related to OS, but we did not find any significant risk variable in RFS for R1 status. In the subgroup analysis, R1 status may have a worse prognosis on patients with more lymph nodes examination. On further investigation, we demonstrated no differences among the four histological types of margin status.In our study, we confirmed that the margin status after sleeve lobectomies was not the risk factor to prognosis. However, patients with more lymph nodes resection should pay attention to the margin status.
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- 2022
4. HIV/AIDS Curability Study, Different Approaches and Drug Combination
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Da-Yong Lu and Ting-Ren Lu
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Microbiology (medical) ,Pharmacology ,Molecular Medicine ,General Medicine - Abstract
AIM: HIV infection is currently an incurable disease characterized by life-long drug utility. Its incurable causality and mechanism are still unknown to us. METHODS: To overcome this therapeutic setback, some breakthroughs should be made by utilizing different approaches. How to plan some experimental and clinical novelty for HIV curability is a modern challenge. In this article, new ideas and approaches for global HIV/AIDS therapeutic strategies are proposed and represented by scientific insights. RESULTS: Pharmaceutical characteristics, herbal medicine, novel drug targets, cutting-edge biotherapy, drug combination, animal modalities, and immune-stimuli for HIV latency, as well as clearance, are highlighted. DISCUSSION: To elucidate our understanding of curative treatment for HIV/AIDS, many new pathological discoveries, expansion, technical advances, and potential drug targets are constructed. After the discovery of novel pathogenesis and therapeutic evolution, HIV/AIDS therapeutic curability may become achievable and a reality. CONCLUSION: Transformation from animal model investigation to widespread therapies for larger volume of human population is a necessity in modern medicine. In this infectious treatment scenario, major breakthroughs in medicine and drug development are anticipated.
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- 2023
5. Reconstruction of the trachea and carina: Surgical reconstruction, autologous tissue transplantation, allograft transplantation, and bioengineering
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Jianghao Ren, Yuanyuan Xu, Guo Zhiyi, Ting Ren, Jiangbin Ren, Kan Wang, Yiqing Luo, Mingyang Zhu, and Qiang Tan
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Pulmonary and Respiratory Medicine ,bioengineering ,Reviews ,regenerative medicine ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Review ,General Medicine ,Plastic Surgery Procedures ,respiratory system ,Allografts ,Transplantation, Autologous ,Trachea ,surgery ,Oncology ,Humans ,tracheal reconstruction ,RC254-282 ,transplantation - Abstract
There have been significant advancements in medical techniques in the present epoch, with the emergence of some novel operative substitutes. However, the treatment of tracheal defects still faces tremendous challenges and there is, as yet, no consensus on tracheal and carinal reconstruction. In addition, surgical outcomes vary in different individuals, which results in an ambiguous future for tracheal surgery. Although transplantation was once an effective and promising method, it is limited by a shortage of donors and immune rejection. The development of bioengineering has provided an alternative for the treatment of tracheal defects, but this discipline is full of ethical controversy and hindered by limited cognition in this area. Meanwhile, progression of this technique is blocked by a deficiency in ideal materials. The trachea together with the carina is still the last unpaired organ in thoracic surgery and propososal of a favorable scheme to remove this dilemma is urgently required. In this review, four main tracheal reconstruction methods, especially surgical techniques, are evaluated, and a thorough interpretation conducted., Tracheal and carinal reconstruction are still challenges in regenerative medicine although medical techniques have achieved significant advancements in the present epoch.
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- 2022
6. Structural characteristics of mixed pectin from ginseng berry and its anti-obesity effects by regulating the intestinal flora
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Ting Ren, Mengran Xu, Shuo Zhou, Jing Ren, Bo Li, Peng Jiang, Hui Li, Wei Wu, Changbao Chen, Meiling Fan, and Lili Jiao
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Structural Biology ,General Medicine ,Molecular Biology ,Biochemistry - Published
- 2023
7. Luteirhabdus pelagi gen. nov., sp. nov., a novel member of the family Flavobacteriaceae, isolated from the West Pacific Ocean
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Li-Li Guo, Li Sun, Yue-Hong Wu, Fan-Xu Meng, Peng Zhou, Wen-Ting Ren, and Xue-Wei Xu
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DNA, Bacterial ,West Pacific Ocean ,Lineage (evolution) ,Biochemistry ,Microbiology ,Genomic analysis ,Genus ,RNA, Ribosomal, 16S ,Botany ,Genetics ,Molecular Biology ,Phylogeny ,Luteirhabdus ,Original Paper ,Pacific Ocean ,Phylogenetic analysis ,biology ,Phylogenetic tree ,Strain (chemistry) ,Bacteroidetes ,Fatty Acids ,Vitamin K 2 ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,16S ribosomal RNA ,Flavobacteriaceae ,Bacterial Typing Techniques ,Bacteria - Abstract
A Gram-stain-negative, aerobic, and yellow-pigmented bacterium, designated A3-108T, was isolated from seawater of the West Pacific Ocean. Cells were non-motile and rod-shaped, with carotenoid-type pigments. Strain A3-108T grew at pH 6.0–8.5 (optimum 6.5) and 15–40 °C (optimum 28 °C), in the presence of 0.5–10% (w/v) NaCl (optimum 1.0%). It possessed the ability to produce H2S. Based on the 16S rRNA gene analysis, strain A3-108T exhibited highest similarity with Aureisphaera salina A6D-50T (90.6%). Phylogenetic analysis shown that strain A3-108T affiliated with members of the family Flavobacteriaceae and represented an independent lineage. The principal fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:1 G, and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The sole isoprenoid quinone was MK-6. The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified aminolipid and one unidentified lipid. The ANIb, in silico DDH and AAI values among the genomes of strain A3-108T and three reference strains were 67.3–71.1%, 18.7–22.1%, and 58.8–71.4%, respectively. The G + C content was 41.0%. Distinctness of the phylogenetic position as well as differentiating chemotaxonomic and other phenotypic traits revealed that strain A3-108T represented a novel genus and species of the family Flavobacteriaceae, for which the name Luteirhabdus pelagi gen. nov., sp. nov. is proposed (type strain, A3-108T = CGMCC 1.18821T = KCTC 82563T).
- Published
- 2021
8. Crude Extracts and Secondary Metabolites of Epichloë bromicola against Phytophthora infestans
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Fan Li, Da‐Hai Mei, Ting Ren, and Qiu‐Yan Song
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Molecular Medicine ,Bioengineering ,General Chemistry ,General Medicine ,Molecular Biology ,Biochemistry - Abstract
Potato late blight caused by Phytophthora infestans is still one of the main factors limiting potato production. Epichloë spp. can provide host plants with various resistances, which makes them show great potential in the biological control of diseases. In this study, we explored the potential biological activity of crude extracts of 20 strains of Epichloë bromicola to control P. infestans. The crude extracts of strains 1 and 8 showed significant antifungal activity with an inhibition rate of 88% and 81%, respectively, and showed different effects on the mycelium morphology of P. infestans observed by scanning electron microscopy. Moreover, the two crude extracts demonstrated an interesting therapeutic and protective effect on potato late blight, and none of the extracts had an adverse effect against zebrafish embryos. A total of 13 metabolites were isolated from the crude extract of strain 8, and these tested compounds showed a weak antifungal effect and the inhibition rate was less than 80%. These findings suggested that strains 1 and 8 have potential for biocontrol of late potato blight.
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- 2022
9. Corrigendum: Thiosulfatihalobacter marinus gen. nov. sp. nov., a novel member of the family Roseobacteraceae, isolated from the West Pacific Ocean
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Yue-Hong Wu, Wen-Ting Ren, Ying-Wen Zhong, Li-Li Guo, Peng Zhou, and Xue-Wei Xu
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General Medicine ,Microbiology ,Ecology, Evolution, Behavior and Systematics - Published
- 2022
10. Antifungal, Repellency, and Insecticidal Activities of Cymbopogon distans and Ruta graveolens Essential Oils and Their Main Chemical Constituents
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Sen Wang, Shi‐Cai Li, Fang‐Shu Cheng, Ting Ren, null Fan‐Li, Da‐Hai Mei, Kun Gao, and Qiu‐Yan Song
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Male ,Ruta ,Insecticides ,Antifungal Agents ,Fungi ,DEET ,Bioengineering ,General Chemistry ,General Medicine ,Biochemistry ,Biological Control Agents ,Insect Repellents ,Oils, Volatile ,Molecular Medicine ,Humans ,Cymbopogon ,Molecular Biology - Abstract
Essential oils produced by Cymbopogon distans and Ruta graveolens with a similar Chinese name could be explained as book fragrance in the Chinese idiom 'shu xiang men di', namely, a wealthy intellectual family according to ancient Chinese. Therefore, volatile oils from these two plants and their main compounds were tested to explore their antifungal, repellent, and insecticide actions. In this study, the essential olis (EO) of C. distans exhibited significant antifungal activity against Rhizopus stolonifera (97 %), Mucor racemosus (97 %), and Trichoderma viride (84 %); its main compounds exhibit interesting activity, such as methyleugenol (87 %) and elemicine (85 %) against T. viride and butyl hydroxytoluene against M. racemosus (90 %) and R. stolonifera (95 %). The EO of R. graveolens and other major chemical constituents showed weak inhibitory effects against other fungi (Aspergillus flavus and Fusarium oxysporum). Then, EO (C. distans and R. graveolens) and its main compounds exhibited obvious repellent activity (more than 85 %) at a concentration of 16 nL/cm
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- 2022
11. Early reoccurrence of traumatic posterior atlantoaxial dislocation without fracture: A case report
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Su-Xia Wang, Jun Fang, Jin-Ting Ren, Li Wang, Zhaode Jiao, and Ying-Hua Sun
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Orthodontics ,Reoccurrence ,Atlantoaxial dislocation ,business.industry ,Transverse ligament ,General Medicine ,Traumatic posterior atlantoaxial dislocation ,03 medical and health sciences ,0302 clinical medicine ,Without fracture ,030220 oncology & carcinogenesis ,Case report ,Fracture (geology) ,Medicine ,030211 gastroenterology & hepatology ,business - Abstract
BACKGROUND In general, atlantoaxial dislocation is rare due to the stability of the C1-C2 complex. Traumatic atlantoaxial dislocations are usually anterior and accompanied by odontoid fractures. Posterior atlantoaxial dislocations are rare, and complete posterior dislocation without associated fracture is even more rare. A case of early recurrence of posterior atlantoaxial dislocation without fracture being in therapy of first closed reduction and then open reduction has not been previously reported. CASE SUMMARY A 45-year-old female presented with traumatic posterior atlantoaxial dislocation (TPAD) of C1-C2 without associated fractures, and Frankel Grade B spinal cord function. She was successfully managed by immediate closed reduction under skull traction. Unexpectedly, 17 d later, re-dislocation was discovered. On day 28, closed reduction was performed as before but failed. Then, open reduction and posterior internal fixation with autologous iliac bone grafts was performed. By 6 mo after surgery, atlantoaxial joint fusion was achieved, and neurological function had recovered to Frankel Grade E. At 12 mo follow-up, she had lost only 15° of cervical rotation, and atlantoaxial complex instability in joint flexing and extending were no longer observed under fluoroscopy. CONCLUSION Early assessment of transverse ligament is critical for TPAD without fracture avoiding re-dislocation after closed reduction.
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- 2021
12. Blockage of ETS homologous factor inhibits the proliferation and invasion of gastric cancer cells through the c-Met pathway
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Xin-Xin Zhou, Wei-Xiang Zhong, Feng Ji, Ya-Mei Wang, Wen-Rui Jiao, Meng-Li Gu, Meng-Ting Ren, Mo-Sang Yu, and Ke-Da Shi
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Small interfering RNA ,Epithelial-Mesenchymal Transition ,C-Met ,ETS homologous factor ,chemistry.chemical_compound ,Cell Movement ,Stomach Neoplasms ,Cell Line, Tumor ,Humans ,Neoplasm Invasiveness ,Propidium iodide ,c-Met ,Cell Proliferation ,Signaling pathway ,Cell growth ,Gastroenterology ,ETS Homologous Factor ,General Medicine ,Basic Study ,Cell cycle ,Molecular biology ,Gene Expression Regulation, Neoplastic ,chemistry ,Antineoplastic target ,Cancer cell ,Signal transduction ,Gastric cancer - Abstract
Background Gastric cancer (GC) is one of the most common and deadliest types of cancer worldwide due to its delayed diagnosis and high metastatic frequency, but its exact pathogenesis has not been fully elucidated. ETS homologous factor (EHF) is an important member of the ETS family and contributes to the pathogenesis of multiple malignant tumors. To date, whether EHF participates in the development of GC via the c-Met signaling pathway remains unclear. Aim To investigate the role and mechanism of EHF in the occurrence and development of GC. Methods The expression of EHF mRNA in GC tissues and cell lines was measured by quantitative PCR. Western blotting was performed to determine the protein expression of EHF, c-Met, and its downstream signal molecules. The EHF expression in GC tissues was further detected by immunohistochemical staining. To investigate the role of EHF in GC oncogenesis, small interfering RNA (siRNA) against EHF was transfected into GC cells. The cell proliferation of GC cells was determined by Cell Counting Kit-8 and colony formation assays. Flow cytometry was performed following Annexin V/propidium iodide (PI) to identify apoptotic cells and PI staining to analyze the cell cycle. Cell migration and invasion were assessed by transwell assays. Results The data showed that EHF was upregulated in GC tissues and cell lines in which increased expression of c-Met was also observed. Silencing of EHF by siRNA reduced the proliferation of GC cells. Inhibition of EHF induced significant apoptosis and cell cycle arrest in GC cells. Cell migration and invasion were significantly inhibited. EHF silencing led to c-Met downregulation and further blocked the Ras/c-Raf/extracellular signal-related kinase 1/2 (Erk1/2) pathway. Additionally, phosphatase and tensin homolog was upregulated and glycogen synthase kinase 3 beta was deactivated. Moreover, inactivation of signal transducer and activator of transcription 3 was detected following EHF inhibition, leading to inhibition of the epithelial-to-mesenchymal transition (EMT). Conclusion These results suggest that EHF plays a key role in cell proliferation, invasion, apoptosis, the cell cycle and EMT via the c-Met pathway. Therefore, EHF may serve as an antineoplastic target for the diagnosis and treatment of GC.
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- 2020
13. Thiosulfatihalobacter marinus gen. nov. sp. nov., a novel member of the family Roseobacteraceae, isolated from the West Pacific Ocean
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Yuehong Wu, Wen-Ting Ren, Ying-Wen Zhong, Li-Li Guo, Peng Zhou, and Xue-Wei Xu
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General Medicine ,Microbiology ,Ecology, Evolution, Behavior and Systematics - Abstract
Two strains (GL-11-2T and ZH2-Y79) were isolated from the seawater collected from the West Pacific Ocean and the East China Sea, respectively. Cells were Gram-stain-negative, strictly aerobic, non-motile and rod-shaped. Cells grew in the medium containing 0.5–7.5 % NaCl (w/v, optimum, 1.0–3.0 %), at pH 6.0–8.0 (optimum, pH 6.5–7.0) and at 4–40 °C (optimum, 30 °C). H2S production occurred in marine broth supplemented with sodium thiosulphate. The almost-complete 16S rRNA gene sequences of the two isolates were identical, and exhibited the highest similarity to Pseudoruegeria aquimaris JCM 13603T (97.5 %), followed by Ruegeria conchae TW15T (97.2%), Shimia aestuarii DSM 15283T (97.1 %) and Ruegeria lacuscaerulensis ITI-1157T (97.0 %). Phylogenetic analysis revealed that the isolates were affiliated with the family Roseobacteraceae and represented an independent lineage. The sole isoprenoid quinone was ubiquinone 10. The principal fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and cyclo-C19 : 0 ω8c. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and diphosphatidylglycerol. The DNA G+C content was 62.3 mol%. The orthologous average nucleotide identity, in silico DNA–DNA hybridization and average amino acid identity values among the genomes of strain GL-11-2T and the reference strains were 73.2–79.0, 20.3–22.5 and 66.0–80.8 %, respectively. Strains GL-11-2ᵀ and ZH2-Y79 possessed complete metabolic pathways for thiosulphate oxidation, dissimilatory nitrate reduction and denitrification. Phylogenetic distinctiveness, chemotaxonomic differences and phenotypic properties revealed that the isolates represent a novel genus and species of the family Roseobacteraceae , belonging to the class Alphaproteobacteria , for which the name Thiosulfatihalobacter marinus gen. nov., sp. nov. (type strain, GL-11–2T=KCTC 82723T=MCCC M20691T) is proposed.
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- 2022
14. Mesobacterium pallidum gen. nov., sp. nov., Heliomarina baculiformis gen. nov., sp. nov. and Oricola indica sp. nov., three novel Alphaproteobacteria members isolated from deep-sea water in the southwest Indian ridge
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Yan-Hui Kong, Wen-Ting Ren, Lin Xu, Hong Cheng, Peng Zhou, Chun-Sheng Wang, Yue-Hong Wu, and Xue-Wei Xu
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General Medicine ,Microbiology ,Ecology, Evolution, Behavior and Systematics - Abstract
Three Gram-staining-negative, aerobic and rod-shaped strains, designated as T40-1T, T40-3T and JL-62T, were isolated from the deep-sea water in the southwest Indian ridge. For strain T40-1T, growth occurred at 15–37 °C (optimum, 28 °C), pH 6.0–9.0 (optimum, pH 7.5) and in the presence of 0.5–5.0 % NaCl (w/v; optimum, 2.0 %). Strain T40-3T could grow at 15–40 °C (optimum, 28 °C), with 0.5–11.0 % NaCl (optimum, 2.0 %, w/v) at pH 6.0–9.5 (optimum, 8.0). The temperature, pH and salinity ranges for growth of strain JL-62T were 15–40 °C (optimum, 30 °C), pH 5.5–9.0 (optimum, pH 7.5–8.0) and 0.5–9.0 % NaCl (w/v; optimum, 4.0 %). Ubiquinone-10 was the sole ubiquinone in all strains, the major fatty acids (>20 %) were summed feature 8 (C18 : 1 ω7c / C18 : 1 ω6c). The major polar lipids of strains T40-1T and T40-3T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Strain JL-62T contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and sulfoquinovosyldiacylglycerol as major polar lipids. Phylogenetic trees based on 16S rRNA gene and core-genomic sequences revealed affiliation of strains T40-1Tand T40-3T to the family Roseobacteraceae and formed two independent clades from other Roseobacteraceae genera, and those two strains had average nucleotide identities of 62.0–72.0 % to their phylogenetically related species which fell into to the genus boundary range, indicating that they represent two novel genera. While strain JL-62T represents a novel species in the genus Oricola belonging to the family Phyllobacteriaceae , which was supported by overall genomic relatedness index calculations. The DNA G+C contents of strains T40-1T, T40-3T and JL-62T were 66.5, 60.1 and 62.1 mol %, respectively. Based on the polyphasic taxonomic data, strains T40-1T (=MCCC M24557T=KCTC 82975T) and T40-3T (=MCCC 1K05135T=KCTC 82976T) are classified as representing two novel genera belonging to the family Roseobacteraceae with the names Mesobacterium pallidum gen. nov., sp. nov. and Heliomarina baculiformis gen. nov., sp. nov. are proposed, and strain JL-62T (=MCCC M24579T=KCTC 82974T) is proposed to represent a novel species within the genus Oricola with the name Oricola indica sp. nov. is proposed.
- Published
- 2022
15. Sirtuin 1 alleviates endoplasmic reticulum stress-mediated apoptosis of intestinal epithelial cells in ulcerative colitis
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Mo-Sang Yu, Xin-Xin Zhou, Meng-Ting Ren, Hang-Hai Pan, Feng Ji, Meng-Li Gu, and Chen-Yan Ding
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Niacinamide ,Apoptosis ,CHOP ,Occludin ,Heterocyclic Compounds, 4 or More Rings ,Mice ,03 medical and health sciences ,0302 clinical medicine ,SRT1720 ,Sirtuin 1 ,Annexin ,medicine ,Animals ,Humans ,Intestinal Mucosa ,Colitis ,Endoplasmic Reticulum Chaperone BiP ,Intestinal barrier ,Caspase 12 ,biology ,Chemistry ,Macrophages ,Endoplasmic reticulum ,Dextran Sulfate ,Gastroenterology ,Epithelial Cells ,General Medicine ,Basic Study ,Endoplasmic Reticulum Stress ,medicine.disease ,Molecular biology ,Coculture Techniques ,Disease Models, Animal ,Ulcerative colitis ,030220 oncology & carcinogenesis ,biology.protein ,Colitis, Ulcerative ,Female ,030211 gastroenterology & hepatology ,Caco-2 Cells ,Transcription Factor CHOP - Abstract
BACKGROUND Sirtuin 1 (SIRT1) is a nicotinamide adenine dinucleotide (NAD+)-dependent protein deacetylase that is involved in various diseases, including cancers, metabolic diseases, and inflammation-associated diseases. However, the role of SIRT1 in ulcerative colitis (UC) is still confusing. AIM To investigate the role of SIRT1 in intestinal epithelial cells (IECs) in UC and further explore the underlying mechanisms. METHODS We developed a coculture model using macrophages and Caco-2 cells. After treatment with the SIRT1 activator SRT1720 or inhibitor nicotinamide (NAM), the expression of occludin and zona occludens 1 (ZO-1) was assessed by Western blot analysis. Annexin V-APC/7-AAD assays were performed to evaluate Caco-2 apoptosis. Dextran sodium sulfate (DSS)-induced colitis mice were exposed to SRT1720 or NAM for 7 d. Transferase-mediated dUTP nick-end labeling (TUNEL) assays were conducted to assess apoptosis in colon tissues. The expression levels of glucose-regulated protein 78 (GRP78), CCAAT/enhancer-binding protein homologous protein (CHOP), caspase-12, caspase-9, and caspase-3 in Caco-2 cells and the colon tissues of treated mice were examined by quantitative real-time PCR and Western blot. RESULTS SRT1720 treatment increased the protein levels of occludin and ZO-1 and inhibited Caco-2 apoptosis, whereas NAM administration caused the opposite effects. DSS-induced colitis mice treated with SRT1720 had a lower disease activity index (P < 0.01), histological score (P < 0.001), inflammatory cytokine levels (P < 0.01), and apoptotic cell rate (P < 0.01), while exposure to NAM caused the opposite effects. Moreover, SIRT1 activation reduced the expression levels of GRP78, CHOP, cleaved caspase-12, cleaved caspase-9, and cleaved caspase-3 in Caco-2 cells and the colon tissues of treated mice. CONCLUSION SIRT1 activation reduces apoptosis of IECs via the suppression of endoplasmic reticulum stress-mediated apoptosis-associated molecules CHOP and caspase-12. SIRT1 activation may be a potential therapeutic strategy for UC.
- Published
- 2019
16. Molecular evolution and phylogenetic relationships of Ligusticum (Apiaceae) inferred from the whole plastome sequences
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Ting Ren, Dengfeng Xie, Chang Peng, Lingjian Gui, Megan Price, Songdong Zhou, and Xingjin He
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Evolution, Molecular ,Genome, Plastid ,Ligusticum ,General Medicine ,Phylogeny ,Apiaceae - Abstract
Background The genus Ligusticum belongs to Apiaceae, and its taxonomy has long been a major difficulty. A robust phylogenetic tree is the basis of accurate taxonomic classification of Ligusticum. We herein used 26 (including 14 newly sequenced) plastome-scale data to generate reliable phylogenetic trees to explore the phylogenetic relationships of Chinese Ligusticum. Results We found that these plastid genomes exhibited diverse plastome characteristics across all four currently identified clades in China, while the plastid protein-coding genes were conserved. The phylogenetic analyses by the concatenation and coalescent methods obtained a more robust molecular phylogeny than prior studies and showed the non-monophyly of Chinese Ligusticum. In the concatenation-based phylogeny analyses, the two datasets yielded slightly different topologies that may be primarily due to the discrepancy in the number of variable sites. Conclusions Our plastid phylogenomics analyses emphasized that the current circumscription of the Chinese Ligusticum should be reduced, and the taxonomy of Ligusticum urgently needs revision. Wider taxon sampling including the related species of Ligusticum will be necessary to explore the phylogenetic relationships of this genus. Overall, our study provided new insights into the taxonomic classification of Ligusticum and would serve as a framework for future studies on taxonomy and delimitation of Ligusticum from the perspective of the plastid genome.
- Published
- 2021
17. Drug Sensitivity Testing for Cancer Therapy, Key Areas
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Da-Yong Lu, Ting-Ren Lu, Nagendra Sastry Yarla, and Bin Xu
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Pharmacology ,General Medicine - Abstract
Aims: Cancer is a high-mortality disease (9.6 million deaths in 2018 worldwide). Given various anticancer drugs, drug selection plays a key role in patient survival in clinical trials. Methods: Drug Sensitivity Testing (DST), one of the leading drug selective systems, was widely practiced for therapeutic promotion in the clinic. Notably, DSTs assist in drug selection that benefits drug responses against cancer from 20-22% to 30-35% over the past two decades. The relationship between drug resistance in vitro and drug treatment benefits was associated with different tumor origins and subtypes. Medical theory and underlying DST mechanisms remain poorly understood until now. The study of the clinical scenario, sustainability and financial support for mechanism and technical promotions is indispensable. Results: Despite the great technical advance, therapeutic prediction and drug selection by DST needs to be miniature, versatility and cost-effective in the clinic. Multi-parameters and automation of DST should be a future trend. Advanced biomedical knowledge and clinical approaches to translating oncologic profiles into drug selection were the main focuses of DST developments. With a great technical stride, the clinical architecture of the DST platform was entering higher levels (drug response testing at any stage of cancer patients and miniaturization of tumor samples). Discuss: The cancer biology and pharmacology for drug selection mutually benefit the clinic. New proposals to reveal more therapeutic information and drug response prediction at genetic, molecular and omics levels should be estimated overall. Conclusion: By upholding this goal of non-invasive, versatility and automation, DST could save the life of several thousand annually worldwide. In this article, new insights into DST novelty and development are highlighted.
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- 2021
18. Corrigendum: Muriiphilus fusiformis gen. nov., sp. nov., a novel non-marine bacterium belonging to the Roseobacter group, and reclassification of Maritimibacter lacisalsi (Zhong et al. 2015) as Muriicola lacisalsi gen. nov., comb. nov
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Yu-Wen Wang, Wen-Ting Ren, Yuan-You Xu, and Xin-Qi Zhang
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General Medicine ,Microbiology ,Ecology, Evolution, Behavior and Systematics - Published
- 2021
19. Development and biological evaluation of AzoBGNU: A novel hypoxia-activated DNA crosslinking prodrug with AGT-inhibitory activity
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Xiaoli Wang, Na Zhang, Ting Ren, Jun Li, Lijiao Zhao, Rugang Zhong, Jiaojiao Wang, Guohui Sun, and Liu Qi
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Male ,Antineoplastic Agents ,Apoptosis ,RM1-950 ,O6-alkylguanine DNA alkyltransferase ,chemistry.chemical_compound ,O(6)-Methylguanine-DNA Methyltransferase ,DU145 ,Drug Development ,DNA Crosslinking ,Cell Line, Tumor ,Spheroids, Cellular ,medicine ,Benzene Derivatives ,Tumor Microenvironment ,Humans ,Prodrugs ,Enzyme Inhibitors ,Cytotoxicity ,DNA Modification Methylases ,Cell Proliferation ,Pharmacology ,drug resistance ,tumor targeting ,Chemistry ,Tumor Suppressor Proteins ,Prostatic Neoplasms ,General Medicine ,DNA interstrand crosslinks ,Prodrug ,Hypoxia (medical) ,DNA Repair Enzymes ,hypoxia-activated prodrug ,Cancer research ,Tumor Hypoxia ,Therapeutics. Pharmacology ,Pharmacophore ,medicine.symptom ,chloroethylnitrosoureas ,DNA ,Alkyltransferase - Abstract
Chloroethylnitrosoureas (CENUs) are an important family of chemotherapies in clinical treatment of cancers, which exert antitumor activity by inducing the formation of DNA interstrand crosslinks (dG-dC ICLs). However, the drug resistance mediated by O6-alkylguanine-DNA alkyltransferase (AGT) and absence of tumor-targeting ability largely decrease the antitumor efficacy of CENUs. In this study, we synthesized an azobenzene-based hypoxia-activated combi-nitrosourea prodrug, AzoBGNU, and evaluated its hypoxic selectivity and antitumor activity. The prodrug was composed of a CENU pharmacophore and an O6-benzylguanine (O6-BG) analog moiety masked by a N,N-dimethyl-4-(phenyldiazenyl)aniline segment as a hypoxia-activated trigger, which was designed to be selectively reduced via azo bond break in hypoxic tumor microenvironment, accompanied with releasing of an O6-BG analog to inhibit AGT and a chloroethylating agent to induce dG-dC ICLs. AzoBGNU exhibited significantly increased cytotoxicity and apoptosis-inducing ability toward DU145 cells under hypoxia compared with normoxia, indicating the hypoxia-responsiveness as expected. Predominant higher cytotoxicity was observed in the cells treated by AzoBGNU than those by traditional CENU chemotherapy ACNU and its combination with O6-BG. The levels of dG-dC ICLs in DU145 cells induced by AzoBGNU was remarkably enhanced under hypoxia, which was approximately 6-fold higher than those in the AzoBGNU-treated groups under normoxia and those in the ACNU-treated groups. The results demonstrated that azobenzene-based combi-nitrosourea prodrug possessed desirable tumor-hypoxia targeting ability and eliminated chemoresistance compared with the conventional CENUs.
- Published
- 2021
20. Physiological and genomic features of Henriciella with the description of Henriciella mobilis sp. nov
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Xue-Wei Xu, Hong Cheng, Yue-Hong Wu, Wen-Ting Ren, Peng Zhou, and Chunsheng Wang
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0106 biological sciences ,Whole genome sequencing ,Genetics ,0303 health sciences ,Phylogenetic tree ,Lineage (evolution) ,Alphaproteobacteria ,General Medicine ,Hyphomonadaceae ,Biology ,biology.organism_classification ,16S ribosomal RNA ,010603 evolutionary biology ,01 natural sciences ,Microbiology ,Algicola ,03 medical and health sciences ,Genus ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology - Abstract
Strains M65T, M69 and JN25 were isolated from seawater of the West Pacific Ocean. Cells of the three strains were Gram-stain-negative, aerobic and rod-shaped. Cells were motile by means of flagella. On the basis of the results of 16S rRNA gene sequence analysis, strains M65T, M69 and JN25 showed the highest 16S rRNA gene sequence similarity to Henriciella algicola MCS27T (98.8 %), followed by Henriciella marina DSM 19595T (98.4 %), Henriciella barbarensis MCS23T (98.4 %), Henriciella pelagia LA220T (98.3 %), Henriciella aquimarina P38T (98.1 %) and Henriciella litoralis SD10T (97.8 %). The 16S rRNA gene sequence similarities among the isolates were 100 %. Phylogenetic analyses revealed that the isolates fell within a cluster comprising the Henriciella species and represented an independent lineage. The average nucleotide identity and in silico DNA–DNA hybridization values between strain M65T and the type strains of Henriciella species were 73.9–85.8 % and 19.9–22.4 %, respectively. The sole respiratory quinone detected in the three isolates was ubiquinone 10. The principal fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C16 : 0. The major polar lipids were glucuronopyranosyldiglyceride, monoglycosyldiglyceride and one unidentified glycolipid. The DNA G+C content was 61.3–61.4 mol%. Phylogenetic distinctiveness, chemotaxonomic differences, together with phenotypic properties, revealed that the isolates could be differentiated from the Henriciella species with validly published names. Therefore, it is proposed that strains M65T, M69 and JN25 represent a novel species of the genus Henriciella , for which the name Henriciella mobilis sp. nov. (type strain, M65T=CGMCC 1.15927T=KCTC 52576T) is proposed.
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- 2021
21. Muriiphilus fusiformis gen. nov., sp. nov., a novel non-marine bacterium belonging to the Roseobacter group, and reclassification of Maritimibacter lacisalsi (Zhong et al. 2015) as Muriicola lacisalsi gen. nov., comb. nov
- Author
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Yuan-You Xu, Xin-Qi Zhang, Yu-Wen Wang, and Wen-Ting Ren
- Subjects
biology ,Phylogenetic tree ,Strain (biology) ,General Medicine ,Roseobacter ,Maritimibacter ,16S ribosomal RNA ,biology.organism_classification ,Microbiology ,Monophyly ,Genus ,Botany ,Ecology, Evolution, Behavior and Systematics ,Bacteria - Abstract
An aerobic, Gram-stain-negative, non-sporulating, flagellated and spindle-like bacterium, designated HY14T, was isolated from a pickle-processing factory wastewater sample. The isolate chemoheterotrophically grew at 4–42 °C (optimum, 35 °C) and pH 5.5–9.0 (optimum, pH 6.0–6.5). Salt was required for growth (0.5–12 % NaCl, w/v). A deep brown and water-soluble uncharacterized pigment was produced when grown in certain media. The predominant fatty acids (>5 %) included C16 : 0, C18 : 1 ω7c, 11-methyl C18 : 1 ω7c and C19 : 0 cyclo ω8c. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, two unidentified phospholipids, two unidentified glycolipids and five unknown lipids. The major isoprenoid quinone was ubiquinone-10. Pairwise alignment based on 16S rRNA gene sequences indicated that strain HY14T had the highest sequence similarity to genera Maritimibacter (95.61–96.05 %) and Boseongicola (95.82 %). Phylogenetic analysis based on core genome illustrated that strain HY14T formed a monophyletic lineage with members of the genus Maritimibacter in the clade of the Roseobacter group in the family Rhodobacteraeceae. The core-gene average amino acid identity used to define bacterial genera by a threshold of 60–80 % was calculated to be 68.56–76.5 % between HY14T and closely related taxa. Several genomic characteristics, such as carrying two RuBisCO-mediated pathways and different osmoprotectant transport pathways, exhibited the genotypic discrepancies of strain HY14T. Based on the polyphasic taxonomic characterization, strain HY14T is considered to represent a novel species of a novel genus belonging to the family Rhodobacteraeceae, for which the name Muriiphilus fusiformis gen. nov., sp. nov. is proposed. The type strain is HY14T (=CGMCC 1.15973T=KCTC 52499T). Maritimibacter lacisalsi (Zhong et al. 2015) is considered to diverge from Maritimibacter alkaliphilus at the genus level, and should be reassigned as a novel genus, for which the name Muriicola lacisalsi gen. nov., comb. nov. is proposed.
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- 2021
22. Cerebral functional abnormalities in patients with nasopharyngeal carcinoma after radiotherapy: an observational magnetic resonance resting-state study
- Author
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Wen-Ting Ren, Ye-Xiong Li, Kai Wang, Li Gao, Jun-Lin Yi, Xiao-Dong Huang, Jing-Wei Luo, Run-Ye Wu, Yong Yang, Jian-Yang Wang, Wen-Qing Wang, Jing-Bo Wang, Feng Ye, Han Ouyang, Jian-Rong Dai, and Li-Min Chen
- Subjects
Oncology ,Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,lcsh:Medicine ,Pilot Projects ,Brain mapping ,03 medical and health sciences ,0302 clinical medicine ,Text mining ,Internal medicine ,Cerebellum ,otorhinolaryngologic diseases ,Medicine ,Humans ,Brain Mapping ,Nasopharyngeal Carcinoma ,medicine.diagnostic_test ,Resting state fMRI ,Radiotherapy ,Functional ,business.industry ,lcsh:R ,Brain ,Magnetic resonance imaging ,General Medicine ,Original Articles ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,Radiation therapy ,stomatognathic diseases ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Observational study ,Female ,business ,Neurocognitive ,030217 neurology & neurosurgery ,MRI - Abstract
Background:. Nasopharyngeal carcinoma (NPC) is sensitive to radiotherapy (RT). However, neurocognitive complications such as memory loss and learning and attention deficits emerge in the survivors of NPC who received RT. It remains unclear how radiation affects patient brain function. This pilot study aimed at finding cerebral functional alterations in NPC patients who have received RT. Methods:. From September 2014 to December 2016, 42 individuals, including 22 NPC patients and 20 normal volunteer controls in National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences, and Peking Union Medical College, were recruited in this study. All patients received resting-state functional magnetic resonance imaging scans and neurocognitive tests 1 day before the initiation of RT (baseline) and 1 day after the completion of RT; the 20 normal controls were also subjected to the same scans and tests. The amplitude of the low-frequency fluctuations (ALFF) in blood oxygen level-dependent signals and functional connectivity (FC) were used to characterize cerebral functional changes. Independent t test, paired t test, and analysis of variances were used to obtain statistical significance across groups. Results:. After RT, NPC patients showed significantly decreased ALFF values in the calcarine sulcus, lingual gyrus, cuneus, and superior occipital gyrus and showed significantly reduced FC mainly in the default mode network (P
- Published
- 2019
23. Ebola Therapeutic Study and Future Directions
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Nagendra Sastry Yarla, Jian-Ding, Ting-Ren Lu, Da-Yong Lu, Hong-Ying Wu, and Bin Xu
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0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,viruses ,Viral pathogenesis ,Context (language use) ,Antiviral Agents ,Disease Outbreaks ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Medicine ,Intensive care medicine ,Pharmacology ,business.industry ,Viral Vaccines ,General Medicine ,Hemorrhagic Fever, Ebola ,Ebolavirus ,Survival Rate ,030104 developmental biology ,Drug development ,Africa ,Molecular Medicine ,business ,030217 neurology & neurosurgery - Abstract
The constant Ebola epidemic outbreaks in Africa arisen in waves of panic worldwide. There is a high mortality rate (30-70%) among the Ebola-infected people in virus- stricken areas. Despite these horrors, the medical capabilities against this deadly viral disease were provided by limited therapeutic agents/options. As a result, several patented agents, biotherapies or prophylactic/therapeutic vaccines need to be reviving into the global markets—including patents of small molecular chemicals, short sequences or oligomers of DNA/RNA, linkages of chemicals with bio-molecules, herbal medicine and so on. In addition, the possible mechanisms of action of these therapeutic options are underway. To promote Ebola biomedical study, the multiple characters of Ebola infections—its origin, pathologic progress, genomic changes, therapeutic context and economic considerations are outlined in this review. Finally, a great difference can be expected after these types of efforts.
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- 2019
24. HIV/AIDS Curable Study: New Forms of Therapeutic Trinity
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Bin Xu, Hong-Ying Wu, Nagendra Sastry Yarla, Ting-Ren Lu, Da-Yong Lu, and Jian Ding
- Subjects
Drug ,medicine.medical_specialty ,Anti-HIV Agents ,media_common.quotation_subject ,medicine.medical_treatment ,HIV Infections ,Immune system ,Acquired immunodeficiency syndrome (AIDS) ,Antiretroviral Therapy, Highly Active ,Drug Discovery ,medicine ,Humans ,Pharmacology (medical) ,Intensive care medicine ,media_common ,AIDS Vaccines ,business.industry ,HIV ,Combination chemotherapy ,General Medicine ,Immunotherapy ,medicine.disease ,Combined Modality Therapy ,Exercise Therapy ,Biological Therapy ,Survival Rate ,Treatment Outcome ,Infectious Diseases ,Drug development ,Infectious disease (medical specialty) ,Medicine, Traditional ,Personalized medicine ,business - Abstract
Background and aims AIDS (acquired immune deficient syndrome), a deadly human infectious disease is caused by HIV (human immunodeficiency viruses) infection. Patient's mortality was eventually reduced to one-fourth by combined chemotherapy (usually 3 chemical drugs simultaneously) than earlier HIV/AIDS treatments (single drug or vaccine) in the clinic. Results Combined treatments against HIV/AIDS are still incurable for all patients despite a high rate of patient's survival. Basic viral pathological study and advancing drug development systems for curable medications are indispensable nowadays and in the future. Conclusion Up to date, therapeutic trinity (combined therapy) against HIV/AIDS is generally among chemical drugs. In this article, several forms of other therapeutic attempts for effectively curing efforts against HIV/AIDS are proposed-including the development of next generation therapeutic HIV vaccines and schedules, new categories of bio-therapy, different pathways of immune-modulation, herbal medicines in general (allopathic, Ayurveda and traditional Chinese medicines), high quality of physical exercises, and especially therapeutic combinations guided by latest medical discovery and principles (new forms of therapeutic trinity against HIV-induced pathogenesis and human mortality).
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- 2019
25. Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα
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Sujuan Xu, Xiaoqiang Ding, Jie Li, Man Guo, Yi Fang, Yang Zhang, Yingxiang Li, Qiang Yang, Jian Zhang, Jifu Jin, Ting Ren, Zhaoxing Sun, Edward Lee, Zhouping Zou, and Xiaoyan Wang
- Subjects
Male ,Article Subject ,Perilipin 2 ,Immunology ,Cellular homeostasis ,Apoptosis ,medicine.disease_cause ,urologic and male genital diseases ,Models, Biological ,Perilipin-2 ,Immunophenotyping ,Kidney Tubules, Proximal ,Mice ,Downregulation and upregulation ,medicine ,Animals ,Humans ,Immunology and Allergy ,PPAR alpha ,chemistry.chemical_classification ,Reactive oxygen species ,Gene knockdown ,biology ,Acute kidney injury ,Hep G2 Cells ,Hydrogen Peroxide ,General Medicine ,Acute Kidney Injury ,RC581-607 ,medicine.disease ,Mitochondria ,Disease Models, Animal ,Oxidative Stress ,Gene Expression Regulation ,chemistry ,Gene Knockdown Techniques ,Cancer research ,biology.protein ,Disease Susceptibility ,Immunologic diseases. Allergy ,Reactive Oxygen Species ,Oxidative stress ,Research Article - Abstract
Renal ischemia-reperfusion (I/R) can induce oxidative stress and injury via the generation of reactive oxygen species (ROS). Renal proximal tubular cells are susceptible to oxidative stress, and the dysregulation of renal proximal tubular cellular homeostasis can damage cells via apoptotic pathways. A recent study showed that the generation of ROS can increase perilipin 2 (Plin2) expression in HepG2 cells. Some evidence has also demonstrated the association between Plin2 expression and renal tumors. However, the underlying mechanism of Plin2 in I/R-induced acute kidney injury (AKI) remains elusive. Here, using a mouse model of I/R-induced AKI, we found that ROS generation was increased and the expression of Plin2 was significantly upregulated. An in vitro study further revealed that the expression of Plin2, and the generation of ROS were significantly upregulated in primary tubular cells treated with hydrogen peroxide. Accordingly, Plin2 knockdown decreased apoptosis in renal proximal tubular epithelial cells treated with hydrogen peroxide, which depended on the activation of peroxisome proliferator-activated receptor α (PPARα). Overall, the present study demonstrated that Plin2 is involved in AKI; knockdown of this marker might limit apoptosis via the activation of PPARα. Consequently, the downregulation of Plin2 could be a novel therapeutic strategy for AKI.
- Published
- 2021
26. Acid mine drainage and sewage impacted groundwater treatment by membrane distillation: Organic micropollutant and metal removal and membrane fouling
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Zulqarnain Fida, Arbab Tufail, Ting Ren, Muhammad Bilal Asif, William E. Price, and Faisal I. Hai
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Environmental Engineering ,0208 environmental biotechnology ,Sewage ,02 engineering and technology ,010501 environmental sciences ,Management, Monitoring, Policy and Law ,Membrane distillation ,01 natural sciences ,Mining ,Waste Management and Disposal ,Groundwater ,0105 earth and related environmental sciences ,Distillation ,business.industry ,Membrane fouling ,Membranes, Artificial ,General Medicine ,Acid mine drainage ,Persulfate ,020801 environmental engineering ,Deposition (aerosol physics) ,Membrane ,Environmental chemistry ,Environmental science ,business - Abstract
Groundwater is the dominant source of freshwater in many countries around the globe, and the deterioration in its quality by contaminants originating from anthropogenic sources raises serious concern. In this study, a scenario where groundwater is contaminated by acid mine drainage (AMD) from mining activities and/or sewage was envisaged, and the performance of a direct contact membrane distillation (DCMD) system was investigated comprehensively for different compositions of the AMD- and sewage-impacted groundwater. Regardless of the composition, MD membrane achieved 98-100% removal of metals and bulk organics, while the removal of the selected micropollutants ranged between 80 and 100%. Effective retention of contaminants by the MD led to their accumulation over time, which affected the hydraulic performance of the MD membrane by reducing the permeate flux by 29-76%. When persulfate (PS)-mediated oxidation process was integrated with the DCMD, degradation of bulk organics (50-71%) and micropollutants (50-100%) by PS reduced their accumulation. Characterisation of the fouling layer revealed the occurrence of membrane scaling that was mainly due to the deposition of iron oxide or oxyhydroxide precipitates. For an identical composition of the AMD- and sewage-impacted groundwater, flux decline was 10% less in PS-assisted DCMD as compared to that in the standalone DCMD. However, this did not prevent the formation of iron oxide scales on MD membrane during the operation of PS-assisted DCMD. This study demonstrates the long-term performance of a standalone and PS-assisted DCMD operated in continuous-flow mode to treat AMD- and sewage-impacted groundwater for the first time.
- Published
- 2020
27. A shift away from mutualism under food-deprived conditions in an anemone-dinoflagellate association
- Author
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Alessandro Moret, Chii-Shiarng Chen, Mario Giordano, Anderson B. Mayfield, Cherilyn Chang, Yu-Ting Ren, Wan-Nan U. Chen, and Shao-En Peng
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0106 biological sciences ,Jellyfish ,Zoology ,Parasitism ,lcsh:Medicine ,Marine Biology ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Aposymbiotic ,Nutrient ,Mutualism ,biology.animal ,Anemone ,030304 developmental biology ,Mutualism (biology) ,0303 health sciences ,geography ,geography.geographical_feature_category ,biology ,Dinoflagellate ,010604 marine biology & hydrobiology ,General Neuroscience ,lcsh:R ,General Medicine ,Coral reef ,biology.organism_classification ,Starvation ,General Agricultural and Biological Sciences ,Exaiptasia pallida - Abstract
The mutualistic symbiosis between anthozoans and intra-gastrodermal dinoflagellates of the family Symbiodiniaceae is the functional basis of all coral reef ecosystems, with the latter providing up to 95% of their fixed photosynthate to their hosts in exchange for nutrients. However, recent studies of sponges, jellyfish, and anemones have revealed the potential for this mutualistic relationship to shift to parasitism under stressful conditions. Over a period of eight weeks, we compared the physiological conditions of both inoculated and aposymbiotic anemones (Exaiptasia pallida) that were either fed or starved. By the sixth week, both fed groups of anemones were significantly larger than their starved counterparts. Moreover, inoculated and starved anemones tended to disintegrate into “tissue balls” within eight weeks, and 25% of the samples died; in contrast, starved aposymbiotic anemones required six months to form tissue balls, and no anemones from this group died. Our results show that the dinoflagellates within inoculated anemones may have posed a fatal metabolic burden on their hosts during starvation; this may be because of the need to prioritize their own metabolism and nourishment at the expense of their hosts. Collectively, our study reveals the potential of this dynamic symbiotic association to shift away from mutualism during food-deprived conditions.
- Published
- 2020
28. Resveratrol synergizes with cisplatin in antineoplastic effects against AGS gastric cancer cells by inducing endoplasmic reticulum stress‑mediated apoptosis and G2/M phase arrest
- Author
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Xin-Xin Zhou, Jinpu Yang, Mo-Sang Yu, Ya-Mei Wang, Sha Liu, Feng Ji, Wen-Rui Jiao, Meng-Li Gu, and Meng-Ting Ren
- Subjects
G2/M cell cycle arrest ,0301 basic medicine ,Cancer Research ,endocrine system diseases ,Eukaryotic Initiation Factor-2 ,Cell ,cisplatin ,resveratrol ,eIF-2 Kinase ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,Cell Line, Tumor ,Enhancer binding ,Antineoplastic Combined Chemotherapy Protocols ,CDC2 Protein Kinase ,medicine ,Humans ,Viability assay ,Cyclin B1 ,Endoplasmic Reticulum Chaperone BiP ,Caspase 12 ,Cell Proliferation ,Cisplatin ,Chemistry ,Kinase ,gastric cancer ,apoptosis ,Cell Cycle Checkpoints ,Articles ,General Medicine ,Cell cycle ,Activating Transcription Factor 4 ,Neoplasm Proteins ,G2 Phase Cell Cycle Checkpoints ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer cell ,endoplasmic reticulum stress ,Cancer research ,Transcription Factor CHOP ,medicine.drug - Abstract
Gastric cancer (GC) is a common gastrointestinal malignancy, and cisplatin (DDP) is an important component of chemotherapeutic regimens for GC. However, the application of DDP is limited by its dose‑dependent systemic toxicity. Resveratrol (RES) is a natural polyphenol compound that has chemopreventive and therapeutic effects against various cancers, including GC. However, whether RES can sensitize GC cells to DDP remains unknown. Following RES/DDP combination treatment, cell viability was determined by Cell Counting Kit‑8 and colony‑forming assays, and cell apoptosis and the cell cycle were detected by FITC‑Annexin V/PI staining assay and PI staining assay, respectively, followed by flow cytometry. Moreover, western blotting was performed to evaluate the protein expression levels, and the intracellular free Ca2+ concentration was determined by a Fluo‑4 AM probe after cell cotreatment with RES and DDP. The present results demonstrated that RES/DDP combination treatment significantly inhibited cell viability, promoted cell apoptosis and induced G2/M phase arrest in AGS cells. In addition, it was determined that RES combined with DDP significantly increased the levels of Bax, cleaved poly‑ADP‑ribose polymerase (PARP), glucose‑regulated protein 78 (GRP78), PRKR‑like ER kinase (PERK), p‑eukaryotic translation initiation factor 2α (p‑eIF2α), CCAAT/enhancer binding protein homologous protein (CHOP) and cleaved caspase‑12, whereas Bcl‑2 expression was downregulated following RES/DDP cotreatment. Moreover, RES/DDP cotreatment significantly upregulated phosphorylated cyclin‑dependent kinase 1 (p‑CDK1, Tyr15), p21Waf1/Cip1 and p27Kip1 protein levels and downregulated Cdc25C protein levels. In conclusion, RES and DDP synergistically inhibited the growth of the gastric adenocarcinoma cell line AGS by inducing endoplasmic reticulum stress‑mediated apoptosis and G2/M phase arrest via activation of the PERK/eIF2α/activating transcription factor 4 (ATF4)/CHOP signaling pathway and caspase‑12 and by inactivating the CDK1‑cyclin B1 complex. These results indicated that RES is a promising adjuvant for DDP during GC chemotherapy.
- Published
- 2020
29. Characteristics and source apportionment of atmospheric volatile organic compounds in Beijing, China
- Author
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Wei Wei, Yun-Ting Ren, Lihui Han, Gan Yang, and Shuiyuan Cheng
- Subjects
China ,010504 meteorology & atmospheric sciences ,Correlation coefficient ,010501 environmental sciences ,Management, Monitoring, Policy and Law ,Combustion ,01 natural sciences ,chemistry.chemical_compound ,Beijing ,Apportionment ,Industry ,Emission inventory ,Gasoline ,Benzene ,Vehicle Emissions ,0105 earth and related environmental sciences ,General Environmental Science ,Air Pollutants ,Volatile Organic Compounds ,General Medicine ,Pollution ,Petrochemical ,chemistry ,Environmental chemistry ,Environmental science ,Environmental Monitoring ,Toluene - Abstract
This paper focused on VOCs and their source apportionment in urban Beijing. Our monitoring measured 52 VOCs in July 2014 and January 2015. The concentration of VOCs was in the range of 14.5~95.2 ppb in July and 2.1~93.1 ppb in January, with the top five compounds of toluene (10.7%), ethane (6.9%), ethylene (6.3%), n-butane (5.7%), and propane (5.6%) in July and ethylene (14.7%), n-butane (14.2%), ethane (9.6%), propylene (8.0%), toluene (7.9%), and benzene (6.9%) in January. The ratio of VOCs to CO reached 0.059 in July and 0.022 in January on average. These differences implied a potential seasonal difference in the VOC source contribution. Then, we conducted a source apportionment study based on 21 major VOCs and CO by using probabilistic matrix factorization (PMF) receptor model. According to the similarity between the PMF analysis profiles and the known source profiles, combustion sources, petrochemical industry sources, solvent utilization sources, and gasoline evaporation sources were identified. The correlation coefficient (R) between the PMF analysis profile and the source profile reached 0.68~0.87 in July and 0.53~0.92 in January. The better apportionment performance in July was mainly due to the use of intensive VOC observations at a 3-h resolution. When we conducted another PMF source apportionment for July based on 12-h resolved concentration input, the R values decreased to 0.47~0.73. Thus, the PMF model depends heavily on the sample number of concentration inputs, and intensive observation is more propitious. Our PMF apportionment results showed that combustion sources, petrochemical industry, solvent utilization, gasoline evaporation, and other sources contributed ambient VOCs in Beijing urban areas of 13.7 ppb, 5.1 ppb, 7.7 ppb, 12.8 ppb, and 3.3 ppb in July and 13.2 ppb, 2.0 ppb, 5.7 ppb, 6.6 ppb, and 1.0 ppb in January, respectively, on a monthly average. These apportionment results match well with the 2013 VOC emission inventory calculated by this study, but also presented significant seasonal differences in the petrochemical industry and gasoline evaporation, in which VOC emissions strongly respond to environmental temperature.
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- 2019
30. ROS activates autophagy in follicular granulosa cells via mTOR pathway to regulate broodiness in goose
- Author
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Ting Ren, Yaping Lou, Lu Han, Jing Yu, Ayong Zhao, Wensai Yu, Songbai Yang, and Yali Wang
- Subjects
0301 basic medicine ,endocrine system ,Cell Survival ,Granulosa cell ,Biology ,Nesting Behavior ,ATG12 ,Superoxide dismutase ,03 medical and health sciences ,Endocrinology ,Food Animals ,Anseriformes ,Follicular phase ,Autophagy ,Animals ,Cells, Cultured ,PI3K/AKT/mTOR pathway ,chemistry.chemical_classification ,Reactive oxygen species ,Granulosa Cells ,TOR Serine-Threonine Kinases ,Hydrogen Peroxide ,General Medicine ,Oxidants ,Cell biology ,Broodiness ,030104 developmental biology ,chemistry ,biology.protein ,Female ,Animal Science and Zoology ,Reactive Oxygen Species ,Signal Transduction - Abstract
Broodiness causes reduced reproductive ability in poultry, but its regulatory mechanism remains poorly understood. ROS (reactive oxygen species) and autophagy are important for follicular development, and the interaction between the two may play a role in regulating broodiness. We examined goose follicles for ROS and oxidation scavenger activities during the egg-laying and broody stages. The follicular granulosa cells were exposed to media containing H2O2, and the interactions between ROS and autophagy in follicular granulosa cells in vitro were analyzed using a Western blot method. We found that the activities of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) were enhanced and the amount of malondialdehyde (MDA) decreased in broody goose follicles. H2O2 inhibited the cell viability and induced autophagy. Furthermore, it was also found that H2O2 regulated autophagy by reducing mTOR and increasing p53; however, H2O2 had no impact on Beclin1 or ATG12. It was also shown that the enhanced autophagy lessened ROS-induced damages. We conclude that ROS and autophagy both played important roles in regulating follicular development to control broodiness in geese, and ROS activated autophagy in follicular granulosa cells via the mTOR pathway.
- Published
- 2017
31. Cancer Bioinformatics for Updating Anticancer Drug Developments and Personalized Therapeutics
- Author
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Rong-Xin Qu, Ting-Ren Lu, Hong-Ying Wu, and Da-Yong Lu
- Subjects
Male ,0301 basic medicine ,Biomedical Research ,Cancer therapy ,Antineoplastic Agents ,Disease ,Bioinformatics ,Sensitivity and Specificity ,03 medical and health sciences ,0302 clinical medicine ,Cancer stem cell ,Neoplasms ,Humans ,Medicine ,Molecular Targeted Therapy ,Precision Medicine ,Pharmacology ,business.industry ,Computational Biology ,Cancer ,General Medicine ,medicine.disease ,Precision medicine ,Anticancer drug ,030104 developmental biology ,030220 oncology & carcinogenesis ,Female ,business - Abstract
Background Last two to three decades, this world witnesses a rapid progress of biomarkers and bioinformatics technologies. Cancer bioinformatics is one of such important omics branches for experimental/clinical studies and applications. Methods Same as other biological techniques or systems, bioinformatics techniques will be widely used. But they are presently not omni-potent. Despite great popularity and improvements, cancer bioinformatics has its own limitations and shortcomings at this stage of technical advancements. Results This article will offer a panorama of bioinformatics in cancer researches and clinical therapeutic applications-possible advantages and limitations relating to cancer therapeutics. A lot of beneficial capabilities and outcomes have been described. As a result, a successful new era for cancer bioinformatics is waiting for us if we can adhere on scientific studies of cancer bioinformatics in malignant- origin mining, medical verifications and clinical diagnostic applications. Conclusion Cancer bioinformatics gave a great significance in disease diagnosis and therapeutic predictions. Many creative ideas and future perspectives are highlighted.
- Published
- 2017
32. Synthesis of 2-alkenyl-3-butoxypropyl guar gum with enhanced rheological properties
- Author
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Ting Ren, Fengsan Zhang, Ying Su, Lei Wang, and Shen Yiding
- Subjects
Rheometer ,Sodium ,chemistry.chemical_element ,Ether ,Chemistry Techniques, Synthetic ,02 engineering and technology ,Alkenes ,010402 general chemistry ,Galactans ,01 natural sciences ,Biochemistry ,Mannans ,chemistry.chemical_compound ,Structural Biology ,Plant Gums ,Polymer chemistry ,Molecular Biology ,Aqueous solution ,Guar gum ,Temperature ,Water ,General Medicine ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Molecular Weight ,chemistry ,Sodium hydroxide ,Attenuated total reflection ,Gravimetric analysis ,Rheology ,0210 nano-technology ,Nuclear chemistry - Abstract
A new guar gum derivative was synthesized though the nucleophilic substitution of sodium hydroxide-activated guar gum with n-butyl glycidyl (BGE) ether. The physicochemical properties of 2-alkenyl-3-butoxypropyl guar gum (ABPG) were characterized by attenuated total reflection Fourier transform infrared spectrometry (ATR-FTIR), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA). The results showed that sodium hydroxide can be effectively substituted with BGE to form the ABPG. The steady and dynamic rheological properties of the aqueous solution and ABPG gel were determined using an RS6000 rheometer. Compared with the guar gum, ABPG enhanced the thickening property and improved the solution stability. The ABPG gel exhibited good temperature resistance and shear stability properties.
- Published
- 2017
33. Anti-tumor and immunomodulatory activities induced by an alkali-extracted polysaccharide BCAP-1 from Bupleurum chinense via NF-κB signaling pathway
- Author
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Tiancheng Lu, Xiangfu Song, Ting Ren, Zhou Zheng, Zhicheng Wang, Haibin Tong, and Fengguo Du
- Subjects
Male ,0301 basic medicine ,Antineoplastic Agents ,02 engineering and technology ,Alkalies ,Polysaccharide ,Plant Roots ,Biochemistry ,Immunomodulation ,Sepharose ,Mice ,03 medical and health sciences ,Polysaccharides ,Structural Biology ,Cell Line, Tumor ,Animals ,MTT assay ,Secretion ,Cytotoxicity ,Molecular Biology ,chemistry.chemical_classification ,biology ,NF-kappa B ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Molecular biology ,Bupleurum ,030104 developmental biology ,chemistry ,Bupleurum chinense ,Phosphorylation ,Signal transduction ,0210 nano-technology - Abstract
Bupleurum chinense is a well-known traditional Chinese medicine. Polysaccharides extracted from medical plants possess multiple healthy benefits. In the present study, an alkali-extracted polysaccharide (BCAP-1) was isolated from Bupleurum chinense, and evaluated its physicochemical features, anti-tumor activities and immunomodulatory effects. BCAP-1 was obtained by alkali-extraction, ethanol precipitation, and fractionation by DEAE-cellulose and Sepharose CL-6B columns. BCAP-1 markedly inhibited Sarcoma 180tumor growth in tumor-bearing mice, and increased the secretion of TNF-α in serum. MTT assay showed that BCAP-1 had no cytotoxicity against S-180 tumor cells. BCAP-1 enhanced the secretion of TNF-α and NO, and the transcripts of TNF-α and iNOS were increased. Meanwhile, BCAP-1 treatment induced the phosphorylation of p65 and decreased the expression of IκB in macrophages. These results suggest that BCAP-1 could activate macrophages through NF-κB signaling pathway, and the anti-tumor effects of BCAP-1 can be achieved by its immunostimulating features.
- Published
- 2017
34. Measurement of O 6 -alkylguanine-DNA alkyltransferase activity in tumour cells using stable isotope dilution HPLC-ESIMS/MS
- Author
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Guohui Sun, Ting Ren, Lijiao Zhao, Tengjiao Fan, and Rugang Zhong
- Subjects
0301 basic medicine ,Spectrometry, Mass, Electrospray Ionization ,Guanine ,DNA damage ,Clinical Biochemistry ,Biochemistry ,Analytical Chemistry ,O(6)-Methylguanine-DNA Methyltransferase ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Limit of Detection ,Tandem Mass Spectrometry ,Cell Line, Tumor ,parasitic diseases ,Humans ,Chromatography, High Pressure Liquid ,Carcinogen ,Chromatography ,Reproducibility of Results ,Cell Biology ,General Medicine ,Blot ,030104 developmental biology ,chemistry ,Cell culture ,Isotope Labeling ,030220 oncology & carcinogenesis ,Cancer cell ,Linear Models ,DNA ,Alkyltransferase - Abstract
The repair of DNA mediated by O(6)-alkylguanine-DNA alkyltransferase (AGT) provides protection against DNA damage from endogenous or exogenous alkylation of the O(6) position of guanine. However, this repair acts as a double-edged sword in cancer treatment, as it not only protects normal cells from chemotherapy-associated toxicities, but also results in cancer cell resistance to guanine O(6)-alkylating antitumour agents. Thus, AGT plays an important role in predicting the individual susceptibility to guanine O(6)-alkylating carcinogens and chemotherapies. Accordingly, it is necessary to establish a quantitative method for determining AGT activity with high accuracy, sensitivity and practicality. Here, we describe a novel nonradioactive method for measuring AGT activity using stable isotope dilution high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). This method is based on the irreversibility of the removal of the O(6)-alkyl group from guanine by AGT and on the high affinity of O(6)-benzylguanine (O(6)-BG) as an AGT substrate. HPLC-ESI-MS/MS was used to measure the AGT activities in cell protein extracts from eight tumour lines, demonstrating that AGT activity was quite variable among different cell lines, ranging from nondetectable to 1021 fmol/mg protein. The experiments performed in intact tumour cells yielded similar results but exhibited slightly higher activities than those observed in cell protein extracts. The accuracy of this method was confirmed by an examination of AGT expression levels using western blotting analysis. To our knowledge, this method is the first mass spectrometry-based AGT activity assay, and will likely provide assistance in the screening of cancer risk or the application of chemotherapies.
- Published
- 2016
35. Impacts of climate change, population growth, and urbanization on future population exposure to long-term temperature change during the warm season in China
- Author
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Ying Li, Wei Zhang, Zhuang Li, Xin Wei, Ting Ren, Yan Zhu, and Jie Liu
- Subjects
China ,Hot Temperature ,Health, Toxicology and Mutagenesis ,Climate Change ,Population ,Climate change ,010501 environmental sciences ,01 natural sciences ,Urbanization ,Environmental Chemistry ,Population growth ,skin and connective tissue diseases ,education ,Population Growth ,0105 earth and related environmental sciences ,education.field_of_study ,Global warming ,Temperature ,Representative Concentration Pathways ,General Medicine ,Pollution ,Projections of population growth ,Environmental science ,sense organs ,Physical geography ,Seasons - Abstract
Climate change is anticipated to raise overall temperatures in the twenty-first century and is likely to intensify population exposure to heat during the warm season and, as a result, increase the risk of heat-related illnesses and deaths. While earlier studies of heat exposure and related health impacts generally focused on the acute effects of short-term exposure indicated by high daily temperature or several days of very hot weather, recent research has suggested that small changes in seasonal average temperature over a long period of time is likely to pose significant health risk as well. Using downscaled climate projections under three Representative Concentration Pathways emission scenarios, high-spatial-resolution population data, and the latest population projections by the United Nations, we aim at projecting future changes in long-term population exposure to summer heat across China in the mid- and late-twenty-first century resulting from global climate change. As the impacts of population growth are often overlooked in projecting future changes in heat exposure, we estimated changes in population-weighted average temperature in the warmest quarter over two future 20-year time periods and compared them with changes in temperature only. Our analysis shows that, nationally, population-weighted average temperature in the warmest quarter is projected to increase by 2.2 °C relative to the current situation in the 2050s and by 2.5 °C in the 2070s, as the result of climate change and population growth. Despite the foreseeable population stabilization in China, changes in population-weighted temperature are projected to be higher than changes in temperature itself for the majority of the 33 provinces (ranging from 0.02 °C to 1.27 °C, or 1% to 126% higher in the 2050s and from 0.02 °C to 1.16 °C, or 1% to 73% higher in the 2070s), with the largest differences mainly occurring in Western China. The impact of urbanization is projected to be relatively insignificant. Our findings provide evidence of possible underestimation of future changes in long-term exposure to summer heat if the effect of population growth is not factored in.
- Published
- 2019
36. Research on massive information query and intelligent analysis method in a complex large-scale system
- Author
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Lin Hui Li, Dai Lin Wang, Yun Lei Lv, and Dan Ting Ren
- Subjects
Information management ,Big Data ,Databases, Factual ,Computer science ,Big data ,Complex system ,Information Storage and Retrieval ,02 engineering and technology ,Computer Communication Networks ,Artificial Intelligence ,0502 economics and business ,Distributed data store ,0202 electrical engineering, electronic engineering, information engineering ,Data Mining ,Humans ,Analysis method ,Information retrieval ,business.industry ,Applied Mathematics ,Scale (chemistry) ,05 social sciences ,Sorting ,General Medicine ,Mathematical Concepts ,Information data ,Computational Mathematics ,Modeling and Simulation ,Job Application ,020201 artificial intelligence & image processing ,General Agricultural and Biological Sciences ,business ,050203 business & management ,Algorithms - Abstract
With the rapid growth of big data and network information, it is particularly important to perform information query and intelligent analysis on unstructured massive data in large-scale complex systems. The existing methods of directly collating, sorting, summarizing, and storing retrieval of documents cannot meet the needs of information management and rapid retrieval of massive data. This paper takes the standardized storage, effective extraction and standardized database construction of massive resume information in social large-scale complex systems as an example, and proposes a massive information query and intelligent analysis method. The method utilizes the semi-structured features of the resume document, constructs the extraction rule model of various resume data to extract the massive resume information. On the basis of HBase distributed storage, with the help of parallel computing technology to optimize the storage and query efficiency, which ensures the intelligent analysis and retrieval of massive resume information. The experimental results show that this method not only greatly improves the extraction accuracy and recall rate of resume information data, but also compared with the traditional methods, there are obvious improvements in the three aspects of massive information retrieval methods, query usage efficiency, and the intelligent analysis of complex systems.
- Published
- 2019
37. Coal seam water infusion for dust control: a technical review
- Author
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Kaixuan Zhang, Jianping Wei, Xiangyu Xu, Ting Ren, and Jian Zhang
- Subjects
Water flow ,Health, Toxicology and Mutagenesis ,Dust particles ,010501 environmental sciences ,complex mixtures ,01 natural sciences ,Occupational Exposure ,Environmental Chemistry ,Humans ,Coal ,Drainage ,Water content ,0105 earth and related environmental sciences ,Water infusion ,Waste management ,business.industry ,Coal mining ,Water ,Dust ,General Medicine ,Pollution ,Coal Mining ,respiratory tract diseases ,Environmental science ,Dust control ,business - Abstract
Coal mine dust continues to be a health and safety issue in underground coal mines. Coal seam water infusion was developed and widely applied in European coal mines for dust control, and was also a common practice in most Chinese coal mines. This method typically involves the infusion of water into the coal seam to increase its moisture content, and therefore reduce dust generation during mining operations. With the availability of other dust control methods such as water spraying systems, the water infusion method has not been considered as a viable means for dust mitigation in modern mines. However, the increase in production output and the deployment of more powerful equipment for coal cutting and transport and intensive gas drainage practices mean that workers could be exposed to more dust contaminations. Whilst the mine operators are committed to suppress and dilute airborne dust particles using these passive measures, there is a need to critically examine and subsequently develop this proactive dust control technology for practical applications in Chinese coal mines. The paper provides a critical review of the water infusion technologies in view of its technological advances and practical application limitations. The methods of water infusion, mechanism of water flow in coal, the role of surfactants and the key parameters influencing the effect of water infusion on dust control are identified and discussed. Existing problems and prospects for water infusion are analysed.
- Published
- 2018
38. HIV Vaccination, is Breakthrough Underway?
- Author
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Ting-Ren Lu, Da-Yong Lu, Bin Xu, Jian Ding, and Hong-Ying Wu
- Subjects
AIDS Vaccines ,0301 basic medicine ,Pharmacology ,medicine.medical_specialty ,business.industry ,Vaccination ,030106 microbiology ,Human immunodeficiency virus (HIV) ,virus diseases ,HIV Infections ,General Medicine ,medicine.disease_cause ,03 medical and health sciences ,Immunology ,Humans ,Medicine ,HIV vaccine ,business ,Intensive care medicine - Abstract
After long defeats-almost no marked breakthrough in HIV vaccination campaign has been observed during the past two decades, and we still have not lost our faiths for the development of highly effective and low risk HIV vaccines. Many effective vaccines have been discovered and will certainly enter into the markets within the next 5 to 10 years. In order to promote HIV vaccine developments and clinical HIV therapeutic improvements, this perspective addresses the good and bad sides of currently available HIV vaccines, discusses many subjects of medical significance and finally provides up-to-date information in the field of HIV studies, in particular regarding vaccine developments and HIV pathogenesis.
- Published
- 2016
39. Influence of the large-small split effect on strategy choice in complex subtraction
- Author
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Lei Mo, Hao Wu, Yanhui Xiang, Ting ting Ren, Rui hong Shang, Li ling Zheng, and Xiaomei Chao
- Subjects
03 medical and health sciences ,0302 clinical medicine ,Arts and Humanities (miscellaneous) ,Process theory ,05 social sciences ,Subtraction ,0501 psychology and cognitive sciences ,General Medicine ,Arithmetic ,Psychology ,030217 neurology & neurosurgery ,050105 experimental psychology ,General Psychology - Abstract
Two main theories have been used to explain the arithmetic split effect: decision-making process theory and strategy choice theory. Using the inequality paradigm, previous studies have confirmed that individuals tend to adopt a plausibility-checking strategy and a whole-calculation strategy to solve large and small split problems in complex addition arithmetic, respectively. This supports strategy choice theory, but it is unknown whether this theory also explains performance in solving different split problems in complex subtraction arithmetic. This study used small, intermediate and large split sizes, with each split condition being further divided into problems requiring and not requiring borrowing. The reaction times (RTs) for large and intermediate splits were significantly shorter than those for small splits, while accuracy was significantly higher for large and middle splits than for small splits, reflecting no speed-accuracy trade-off. Further, RTs and accuracy differed significantly between the borrow and no-borrow conditions only for small splits. This study indicates that strategy choice theory is suitable to explain the split effect in complex subtraction arithmetic. That is, individuals tend to choose the plausibility-checking strategy or the whole-calculation strategy according to the split size.
- Published
- 2016
40. Analysis of the complete mitochondrial genome of the Zhedong White goose and characterization of NUMTs: Reveal domestication history of goose in China and Euro
- Author
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Ke He, Ting Ren, Ayong Zhao, and Shiri Liang
- Subjects
0106 biological sciences ,0301 basic medicine ,China ,Anser cygnoides ,Mitochondrial DNA ,Molecular Sequence Data ,DNA, Mitochondrial ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,Goose ,Phylogenetics ,biology.animal ,Geese ,Genetics ,Animals ,Clade ,Domestication ,Phylogeny ,Base Sequence ,Phylogenetic tree ,biology ,Genetic Variation ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,Biological Evolution ,Europe ,030104 developmental biology ,Haplotypes ,Evolutionary biology ,Animals, Domestic ,Genome, Mitochondrial ,Numt - Abstract
To understand the phyletic evolution of geese, the complete mitogenome of the Zhedong goose was sequenced for the first time. It is composed of 37 genes and 1 control region, and the structure and arrangement of all genes sequenced are identical to those of other goose breeds. We confirmed the accuracy of the mitogenome sequence through RT-PCR and found numts from amplification in genomic DNA. Comparisons of the phylogenetic trees and sequences of geese that were suggested a clade of Chinese geese, except the Yili goose, were classified in the Euro clade. Several breed-specific mutations and Chinese breed-specific mutations were found. Our results suggest that Chinese geese evolved from the swan goose, splitting from their common ancestors at different times, which was consistent with studies before. Furthermore, numts in most genes of Zhedong goose clustered with European geese in the phylogenetic tree, suggesting that the haplotypes in the Euro clade might be more ancient. However, the mitogenome of the swan goose shows distinctive evolutionary positions in some genes, which suggest its unclear relationship with Chinese geese and European geese. The current study added to the understanding of the evolution of geese and provided evidence that the typing of numts is an encouraging way for the evolutionary study of geese and the mitochondrial genomes of geese deserve further investigation.
- Published
- 2016
41. Effectiveness and safety of acupuncture for anxiety disorder of coronavirus disease 2019
- Author
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Ting Ren, Hongyu Li, Luwen Zhu, Yu Zhang, and Qiang Tang
- Subjects
Research design ,medicine.medical_specialty ,Pneumonia, Viral ,Acupuncture Therapy ,MEDLINE ,Cochrane Library ,law.invention ,Betacoronavirus ,03 medical and health sciences ,0302 clinical medicine ,systematic review ,Meta-Analysis as Topic ,Randomized controlled trial ,law ,Study Protocol Systematic Review ,Acupuncture ,medicine ,Humans ,protocol ,030212 general & internal medicine ,Pandemics ,SARS-CoV-2 ,business.industry ,COVID-19 ,General Medicine ,anxiety ,medicine.disease ,Anxiety Disorders ,Treatment Outcome ,Research Design ,030220 oncology & carcinogenesis ,Meta-analysis ,Family medicine ,Coronavirus Infections ,business ,acupuncture ,Anxiety disorder ,Research Article ,Systematic Reviews as Topic - Abstract
Background: Anxiety disorder places a heavy burden in the clinical treatment of patients of COVID-19. Acupuncture is a recommended treatment of COVID-19 in China, and clinical researches showed the effectiveness of acupuncture. We will conduct this systematic review and meta-analysis to assess the effectiveness and safety of acupuncture for COVID-19. Methods: Electronic databases of Medline, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI), Chinese Biomedical literature Database (CBM), Chinese Scientific and Journal Database (VIP), and Wan Fang database (Wanfang) will be searched for randomized controlled trials of acupuncture for anxiety disorder of COVID-19 from inception of the database to August 10, 2020. Two reviewers will screen studies, collect information independently. We will utilize RevMan 5.3 for meta-analysis. Results: We will publish the study result to a peer-reviewed journal. Conclusion: This study will contribute to provide high-quality evidence of acupuncture for anxiety disorder of COVID-19
- Published
- 2020
42. Comparative Plastid Genomes of Primula Species: Sequence Divergence and Phylogenetic Relationships
- Author
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Tao Zhou, Zhan-Lin Liu, Ting Ren, and Yanci Yang
- Subjects
0301 basic medicine ,DNA, Plant ,Genome, Plastid ,repeat ,Biology ,Genome ,Catalysis ,Article ,Inorganic Chemistry ,Evolution, Molecular ,lcsh:Chemistry ,03 medical and health sciences ,Monophyly ,sequence divergence ,Phylogenetics ,plastid genome ,phylogenetic relationship ,Primula ,Androsace ,Plastids ,Physical and Theoretical Chemistry ,Clade ,Molecular Biology ,lcsh:QH301-705.5 ,Spectroscopy ,Phylogeny ,Phylogenetic tree ,Organic Chemistry ,food and beverages ,General Medicine ,Sequence Analysis, DNA ,biology.organism_classification ,Computer Science Applications ,Primulaceae ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,Evolutionary biology - Abstract
Compared to traditional DNA markers, genome-scale datasets can provide mass information to effectively address historically difficult phylogenies. Primula is the largest genus in the family Primulaceae, with members distributed mainly throughout temperate and arctic areas of the Northern Hemisphere. The phylogenetic relationships among Primula taxa still maintain unresolved, mainly due to intra- and interspecific morphological variation, which was caused by frequent hybridization and introgression. In this study, we sequenced and assembled four complete plastid genomes (Primula handeliana, Primula woodwardii, Primula knuthiana, and Androsace laxa) by Illumina paired-end sequencing. A total of 10 Primula species (including 7 published plastid genomes) were analyzed to investigate the plastid genome sequence divergence and their inferences for the phylogeny of Primula. The 10 Primula plastid genomes were similar in terms of their gene content and order, GC content, and codon usage, but slightly different in the number of the repeat. Moderate sequence divergence was observed among Primula plastid genomes. Phylogenetic analysis strongly supported that Primula was monophyletic and more closely related to Androsace in the Primulaceae family. The phylogenetic relationships among the 10 Primula species showed that the placement of P. knuthiana–P. veris clade was uncertain in the phylogenetic tree. This study indicated that plastid genome data were highly effective to investigate the phylogeny.
- Published
- 2018
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43. Synthesis, cytotoxic evaluation and target identification of thieno[2,3-d]pyrimidine derivatives with a dithiocarbamate side chain at C2 position
- Author
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Bin Peng, Ji Liao, You-Shan Li, Xingzhi Xu, Jing Li, Ting-Ting Ren, Hailong Wang, Fu-Cheng Wang, Sheng-Li Cao, Shibin Xu, Chao-Rui Yang, Wei Jiang, Zheng Li, and Guo Wang
- Subjects
Cell cycle checkpoint ,Pyrimidine ,Antineoplastic Agents ,01 natural sciences ,chemistry.chemical_compound ,Structure-Activity Relationship ,Biotin ,Thiocarbamates ,Drug Discovery ,Tumor Cells, Cultured ,Cytotoxic T cell ,Humans ,Dithiocarbamate ,Cytotoxicity ,Cell Proliferation ,Pharmacology ,chemistry.chemical_classification ,biology ,Dose-Response Relationship, Drug ,Molecular Structure ,010405 organic chemistry ,Organic Chemistry ,Cell Cycle ,General Medicine ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Tubulin ,Pyrimidines ,Biochemistry ,chemistry ,biology.protein ,Target protein ,Drug Screening Assays, Antitumor - Abstract
Two series of thieno[2,3- d ]pyrimidine derivatives bearing a dithiocarbamate side chain at the C2 position were synthesized and evaluated for cytotoxic activity in human lung cancer A549 and colon cancer HCT-116 cell lines. Compound 3n exhibited the most cytotoxic effect on A549 cells with an IC 50 value of 4.87 μM, inducing a cell cycle arrest at G2/M phase and activating the spindle assembly checkpoint (SAC). To identify the target protein(s) of 3n , we incorporated biotin with 3n through a three-carbon chain and an amide bond to synthesize probe 10 . The targeted proteins were pulled down from the A549 total cell lysate by biotin-streptavidin affinity purification and analyzed by mass spectrometry. Tubulin was the only protein identified, which is related to the SAC and directly binds to probe 10 both in vivo and in vitro . Furthermore, compound 3n inhibited tubulin polymerization in vitro in a dose-dependent manner, competed with taxol in binding to tubulin, exerting cytotoxic activity toward taxol-resistant A549 cells. These results demonstrate that thieno[2,3- d ]pyrimidine derivative 3n exhibits cytotoxicity in cancer cells by targeting tubulin to activate the SAC and potentially acts as a therapeutic lead compound for taxol-resistant cancers.
- Published
- 2018
44. Keep up the pace of drug development evolution and expenditure
- Author
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Min Huang, Nagendra Sastry Yarla, En-Hong Chen, Ting-Ren Lu, Da-Yong Lu, Jian Ding, Bin Xu, and Hong Zhu
- Subjects
Drug development ,Economic policy ,General Medicine ,Business ,Pace - Published
- 2018
45. New Perspectives of HIV/AIDS Therapy Study
- Author
-
Bin Xu, Ting-Ren Lu, Da-Yong Lu, Hong-Ying Wu, and Jin-Yu Che
- Subjects
medicine.medical_specialty ,Anti-HIV Agents ,medicine.drug_class ,Human immunodeficiency virus (HIV) ,HIV Infections ,Drug resistance ,Disease ,medicine.disease_cause ,Antiviral Agents ,Patents as Topic ,Acquired immunodeficiency syndrome (AIDS) ,Antiretroviral Therapy, Highly Active ,Drug Resistance, Viral ,Drug Discovery ,medicine ,Humans ,Pharmacology (medical) ,Intensive care medicine ,AIDS Vaccines ,Acquired Immunodeficiency Syndrome ,business.industry ,Outbreak ,General Medicine ,medicine.disease ,Infectious Diseases ,Infectious disease (medical specialty) ,Immunology ,Antiviral drug ,business - Abstract
HIV/AIDS (acquired immune deficient syndrome), a human infectious disease was once listed as the No. 1 disease killer in US (1993). After the invention of antiviral drug cocktails-high active anti-retroviral therapy (HAART), most HIV-infected patients can survive much longer than with single antiviral drugs or vaccines alone. However, it turns out to be a chronic disease owing to being incapable to eradicate HIV from infectious patients. Furthermore, potential newly outbreak of HIV epidemics caused by widespread drug-resistance or viral mutations is still looming over the globe. In order to counteract these drawbacks and possibilities of HAART, many hurdles must be passed. More creative and revolutionary ideas and worldwide cooperation efforts among academics, drug developers and governmental funding bodies must be encouraged and promoted. In this perspective, many important drawbacks and weaknesses relating to HIV/AIDS therapies are outlined and possible future solutions are highlighted.
- Published
- 2015
46. Drug Combination in Clinical Cancer Treatments
- Author
-
Hong Zhu, Ting-Ren Lu, Da-Yong Lu, Jian Ding, Hong-Ying Wu, Bin Xu, and Nagendra Sastry Yarla
- Subjects
0301 basic medicine ,Drug ,medicine.medical_specialty ,media_common.quotation_subject ,Alternative medicine ,Antineoplastic Agents ,Drug resistance ,Traditional Chinese medicine ,Pharmacology ,03 medical and health sciences ,Cancer stem cell ,Neoplasms ,Antineoplastic Combined Chemotherapy Protocols ,Medicine ,Humans ,Precision Medicine ,Intensive care medicine ,media_common ,Neoplasm Staging ,business.industry ,Cancer ,General Medicine ,medicine.disease ,Molecular diagnostics ,Anticancer drug ,030104 developmental biology ,business - Abstract
Aim The modality of anticancer drug combinations needs to be renovated from empirical into technical-supportive systems. Methods To challenge past therapeutic routines, the new landscape may be established. Among the different areas of anticancer drug combination study, research in the fields of medical study is the most important one-including disciplinary of therapeutics in different cancer stages, modern genetic/ molecular diagnostics, cancer bioinformatics, traditional Chinese medicine, mathematical data analysis, therapeutic toxicity monitor, personalized cancer medicine and so on. Discussion This article addresses these types of cancer therapeutic management systems for clinical anticancer drug combination utilities. Conclusion Future cancer drug combinational studies and clinical optimums must be implemented.
- Published
- 2017
47. HAART in HIV/AIDS Treatments: Future Trends
- Author
-
Bin Xu, Jian Ding, Ting-Ren Lu, Da-Yong Lu, Hong-Ying Wu, and Nagendra Sastry Yarla
- Subjects
0301 basic medicine ,Microbiology (medical) ,Drug ,Male ,medicine.medical_specialty ,medicine.drug_class ,Anti-HIV Agents ,media_common.quotation_subject ,Chemistry, Pharmaceutical ,Integrase inhibitor ,HIV Infections ,Disease ,Virus ,03 medical and health sciences ,Drug Delivery Systems ,Acquired immunodeficiency syndrome (AIDS) ,Antiretroviral Therapy, Highly Active ,Drug Discovery ,medicine ,Humans ,Life saving ,Intensive care medicine ,media_common ,Disease Reservoirs ,Pharmacology ,Acquired Immunodeficiency Syndrome ,business.industry ,virus diseases ,General Medicine ,medicine.disease ,030104 developmental biology ,Pharmacogenetics ,Pharmacogenomics ,Immunology ,Molecular Medicine ,Female ,Antiviral drug ,business - Abstract
AIDS (acquired immune deficient syndrome) is a deadly human viral infectious disease caused by HIV (human immune-deficient virus) infection. Almost every AIDS patient losses his/her life before mid 1990s. AIDS was once the 1st disease killer in US (1993). After one decade hard work, antiviral drug cocktails-high active anti-retroviral therapy (HAART) have been invented for almost all HIV infection treatments. Due to the invention of HAART, 80-90% HIV/AIDS patients still effectively response to HAART for deadly AIDS episode controls and life saving. Yet, this type of HIV therapeutics is incurable. HIV/AIDS patients need to take HAART medications regularly and even life-long. To counteract this therapeutic drawback, more revolutionary efforts (different angles of therapeutic modes/attempts) are urgently needed. In this article, the major progresses and drawbacks of HIV/AIDS chemotherapy (HAART) to HIV/AIDS patients have been discussed. Future trends (updating pathogenesis study, next generations of drug developments, new drug target discovery, different scientific disciplinary and so on) are highlighted.
- Published
- 2017
48. Antimetastatic therapy at aberrant sialylation in cancer cells, a potential hotspot
- Author
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En-Hong Chen, Bin Xu, Hong Zhu, Hong-Ying Wu, Shu-Yun Wu, Ting-Ren Lu, Da-Yong Lu, Jian Ding, and Nagendra Sastry Yarla
- Subjects
Cancer cell ,Cancer research ,General Medicine ,Biology - Published
- 2017
49. Study on the interaction of taiwaniaquinoids with FTO by spectroscopy and molecular modeling
- Author
-
Jinqian Wang, Lijiao Zhang, Junbiao Chang, Ruiyong Wang, Chuanjun Song, and Ting Ren
- Subjects
Models, Molecular ,Molecular model ,Analytical chemistry ,Molecular Conformation ,Alpha-Ketoglutarate-Dependent Dioxygenase FTO ,Molecular Dynamics Simulation ,010402 general chemistry ,01 natural sciences ,Fluorescence spectroscopy ,Structural Biology ,Molecule ,Spectroscopy ,Molecular Biology ,Quenching (fluorescence) ,Binding Sites ,010405 organic chemistry ,Chemistry ,Spectrum Analysis ,Intermolecular force ,General Medicine ,Electrostatics ,0104 chemical sciences ,Molecular Docking Simulation ,Crystallography ,Thermodynamics ,Absorption (chemistry) ,Diterpenes ,Protein Binding - Abstract
In this work, an attempt has been made to study the interaction of four taiwaniaquinoids with fat mass and obesity-associated protein (FTO) by UV-vis absorption, fluorescence spectroscopy, and molecular docking techniques. The results indicated that taiwaniaquinoids effectively quenched the intrinsic fluorescence of FTO via static quenching. According to the binding constants and thermodynamic parameters at three different temperatures, the hydrophobic force and electrostatic interactions appeared be the predominant intermolecular forces in stabilizing the complex. Results revealed that W-4 was the strongest quencher and W-3 was the weakest. The results of synchronous and three-dimensional fluorescence spectra showed that the conformation of FTO was changed. In addition, the influence of molecular structure on the quenching effect has been investigated.
- Published
- 2016
50. Formal and Informal Institutional Constraints on Firm Innovative Performance
- Author
-
Ting Ren, Hongyan Yang, Cuifen Weng, and Xuanye Li
- Subjects
Survey data collection ,General Medicine ,Business ,China ,Degree (music) ,Industrial organization - Abstract
We study the impact of formal and informal institutional constraints on firm innovation, using 2012 World Bank Enterprise Survey data in China. We propose a framework identifying both the degree of...
- Published
- 2019
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