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As SARS-CoV-2 variants of concern (VOC) reduce the effectiveness of existing anti-COVID therapeutics, it is increasingly critical to identify highly potent neutralizing antibodies (nAbs) that bind to conserved regions across multiple variants, especially beta, delta, and omicron variants. Using single-cell sequencing with biochemical methods and pseudo-typed virus neutralization experiments, here we report the characterization of a potent nAb BD-218, identified from an early screen of patients recovering from the original virus. We have determined the cryo-EM structure of the BD-218/spike protein complex to define its epitope in detail, which revealed that BD-218 interacts with a novel epitope on the receptor-binding domain (RBD) of the spike protein. We concluded that BD-218 is a highly effective and broadly active nAb against SARS-CoV-2 variants with promising potential for therapeutic development.

The present study aimed to identify novel diagnostic differentially expressed microRNAs (miRNAs/miRs) in order to understand the molecular mechanisms underlying hepatocellular carcinoma. The expression data of miRNA and mRNA were downloaded for differential expression analysis. Optimal diagnostic differentially expressed miRNA biomarkers were identified via a random forest algorithm. Classification models were established to distinguish patients with hepatocellular carcinoma and normal individuals. A regulatory network between optimal diagnostic differentially expressed miRNA and differentially expressed mRNAs was then constructed. The {"type":"entrez-geo","attrs":{"text":"GSE63046","term_id":"63046"}}GSE63046 dataset and in vitro experiments were used to validate the expression of the optimal diagnostic differentially expressed miRNAs identified. In addition, diagnostic and prognostic analyses of optimal diagnostic differentially expressed miRNAs were performed. In total, 14 differentially expressed miRNAs (all upregulated) and 2,982 differentially expressed mRNAs (1,989 upregulated and 993 downregulated) were identified. hsa-miR-10b-5p, hsa-miR-10b-3p, hsa-miR-224-5p, hsa-miR-183-5p and hsa-miR-182-5p were considered as the optimal diagnostic biomarkers for hepatocellular carcinoma. The mRNAs targeted by these five miRNAs included secreted frizzled related protein 1 (SFRP1), endothelin receptor type B (EDNRB), nuclear receptor subfamily 4 group A member 3 (NR4A3), four and a half LIM domains 2 (FHL2), NK3 homeobox 1 (NKX3-1), interleukin 6 signal transducer (IL6ST) and forkhead box O1 (FOXO1). ‘Bile acid biosynthesis and cholesterol’ was the most enriched signaling pathways of these target mRNAs. The expression validation of the five miRNAs was consistent with the present bioinformatics analysis. Notably, hsa-miR-10b-5p and hsa-miR-10b-3p had a significant prognosis value for patients with hepatocellular carcinoma. In conclusion, the five differentially expressed miRNAs may be considered as diagnostic biomarkers for patients with hepatocellular carcinoma. In addition, the differential expression levels of the targets of these five mRNAs, including SFRP1, EDNRB, NR4A3, FHL2, NKX3−1, IL6ST and FOXO1, may be involved in hepatocellular carcinoma tumorigenesis.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a major impact on global human health. During the spread of SARS-CoV-2, weakened host immunity and the use of vaccines with low efficacy may result in the development of more-virulent strains or strains with resistance to existing vaccines and antibodies. The prevalence of SARS-CoV-2 mutant strains differs between regions, and this variation may have an impact on the effectiveness of vaccines. In this study, an epidemiological investigation of SARS-CoV-2 in Portugal was performed, and the VSV-ΔG-G* pseudovirus system was used to construct 12 spike protein epidemic mutants, D614G, A222V+D614G, B.1.1.7, S477N+D614G, P1162R+D614G+A222V, D839Y+D614G, L176F+D614G, B.1.1.7+L216F, B.1.1.7+M740V, B.1.258, B.1.258+L1063F, and B.1.258+N751Y. The mutant pseudoviruses were used to infect four susceptible cell lines (Huh7, hACE2-293T-293T, Vero, and LLC-MK2) and 14 cell lines overexpressing ACE2 from different species. Mutant strains did not show increased infectivity or cross-species transmission. Neutralization activity against these pseudoviruses was evaluated using mouse serum and 11 monoclonal antibodies. The neutralizing activity of immunized mouse serum was not significantly reduced with the mutant strains, but the mutant strains from Portugal could evade nine of the 11 monoclonal antibodies tested. Neutralization resistance was mainly caused by the mutations S477N, N439K, and N501Y in the spike-receptor binding domain. These findings emphasize the importance of SARS-CoV-2 mutation tracking in different regions for epidemic prevention and control. Supplementary Information The online version contains supplementary material available at 10.1007/s00705-021-05327-0.

This paper reviews the study of wind induced vibration of transmission power tower-line system. Each fields of research of transmission power tower-line system —analysis model, dynamic characteristic, wind response and vibration control —are systematically introduced. the problem that exist in this stage and the development trend are summarized in the conclusions.

The impacts of wind disturbance on voltage regulation and frequency regulation of power system were studied. The opinion that the regulation of power flow on the tie lines between the grids constrains the integrated capacity of wind power was put forward. Based on the real condition of Inner Mongolia power grid, an engineering practical method was put forward to calculate the integrated capacity of wind power under this constraint. The relationship between wind power and spinning reserve and the impacts of other related factors on the capacity of wind power were studied as well. The impact of wind disturbance on voltage stability where the wind farms are located was studied.

With the advent of the era of knowledge economy, Chinese power enterprises realize that intellectual property is becoming an important intangible asset. How to optimize the management organizational structure of intellectual property right has received increasing attention. This article analyzes the present situation of Chinese power enterprises, sums up the characteristics of advanced enterprises’ management organization structure, and provides an idea of optimization.

In order to promote the fusion of lime in semi-steelmaking, fluorite was used in converter steelmaking to improve the melting slag ratio, while fluorite is very harmful. In order to make best use of the metallurgical slag and improve the scorification problems in semi-steelmaking, PANGGANG Research Institute developed a new type high alumina composite slag, and accomplished industrial application which also achieved good results. They found that this new type slag can improve the melting slag ratio greatly and decrease the times of “re-drying” and “slopping” throughout the converter steelmaking, the slag sate can be good controlled, at the same time the content radio of TFe decreased significantly.

Steelmaking Plant of PZH Steel improves the hitting ratio of independent converter model, by optimizing the terminal controlling model of molten steel, stabilizing the charging load in BOF, accelerating the turnover rapid of ladle, improving the quality of semi-steel into the furnace and optimizing the management of production organization. So the ratio of the direct-tapping is improved and increases to more than 50% now from 25% on average in 2011.

This paper firstly analyzed the desulfurization mechanism of magnesium compound desulfurizer and compound desulfurizer. It is thought that the compound desulfurizer which contains a certain amount of magnesium is better used than the one does not contain. There is also the statistical analysis about these two type desulfurizers in practical Producing. The practical application of contrast verifies the theoretical analysis.The magnesium compound desulfurizer has better performance used in the effect of desulfurization, the consumption of desulfurizer, iron loss, process-time, the loss of hot metal temperature and the lance life than compound desulfurizer.

Sixteen cases of tuberculosis of the knee treated by total knee arthroplasty with followup of 3.4 to 11 years were reviewed. Eight cases were treated with antituberculous chemotherapy for 2 to 20 months before and 1 year after the arthroplasty. Another 8 cases were not diagnosed primarily and therefore received only postoperative antituberculous chemotherapy. Five cases had a recurrence of tuberculosis. Four of these 5 cases did not receive preoperative antituberculous treatment. The infection in 1 case was controlled satisfactorily with chemotherapy alone, and for the other 3 cases, chemotherapy was supported by surgical debridements. The last case of recurrent infection occurred in a patient who had received long- standing corticosteroid therapy. He required an excisional arthroplasty to control the infection. The mean functional knee score was 30.5 points before surgery and 82.6 points at the time of the last followup. These results suggest that arthroplasty may be formed for knees badly damaged with tuberculous infection, and good results may be expected for those patients who have received effective antituberculous chemotherapy both before and after surgery. There is a substantial risk of reactivation of tuberculous infection for those patients not treated before surgery or for patients dependent on corticosteroids.

This study assessed the healing potential of longitudinal or transverse incisions in the peripheral, central, and inner rim regions of rabbit medial menisci. Longitudinal and transverse incisions were made in 48 male New Zealand white rabbits. The incisions were either repaired with sutures or left open. The effect of joint motion on repair was also evaluated. In one group, longitudinal incisions were made in the peripheral, central, and inner rim regions of the meniscus. Some of these incisions were sutured, whereas others were not repaired. In another group, transverse incisions were made in the meniscus, extending from the inner rim to the middle of the meniscus or to the peripheral attachment (radial tear). Again, the lesions were left open or repaired. The effect of joint motion on healing was assessed by immobilizing several knees from each group with Kirschner wires temporarily for ten weeks. Suture of the meniscus incision did not substantially affect the rate of healing. When the healing rates of menisci at the periphery, body, and rim were compared, incisions at the periphery healed significantly better than incisions at the meniscal body and central rim regions. K-wire immobilization did not substantially affect the rate of healing. The difference in healing with regard to the type of incision, either longitudinal or transverse, was not statistically significant.

This is a report of a subchondral cyst of the entire fibular head in a 24-year-old woman, and a survey of the literature including pathogenesis, histopathology, and management. Subchondral bone cyst in this location seems not to have been reported previously. The treatment was excision of the head of the fibula.