1. Nocardioides daeguensis sp. nov., a nitrate-reducing bacterium isolated from activated sludge of an industrial wastewater treatment plant
- Author
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Long Jin, Yingshun Cui, Kwang Kyu Kim, Joonhong Park, Sahastranshu Sinha, Sung Taik Lee, Sung-Geun Woo, Myung Suk Kang, Myung-Jin Lee, and Jangho Lee
- Subjects
DNA, Bacterial ,Molecular Sequence Data ,Peptidoglycan ,Wastewater ,Diaminopimelic Acid ,medicine.disease_cause ,Microbiology ,Phylogenetics ,RNA, Ribosomal, 16S ,Actinomycetales ,Republic of Korea ,Botany ,medicine ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Base Composition ,Sewage ,Strain (chemistry) ,biology ,Phylogenetic tree ,Nocardioides nitrophenolicus ,Fatty Acids ,Nucleic Acid Hybridization ,Vitamin K 2 ,Sequence Analysis, DNA ,General Medicine ,Ribosomal RNA ,Nocardioides daeguensis ,biology.organism_classification ,16S ribosomal RNA ,Bacterial Typing Techniques ,Bacteria - Abstract
A Gram-reaction-positive, rod-shaped, non-spore-forming bacterium (strain 2C1-5T) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6 % similarity), N. kongjuensis (98.5 %), N. caeni (98.4 %), N. simplex (98.3 %), N. aromaticivorans (98.1 %) and N. ginsengisoli (97.5 %); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3 %. Strain 2C1-5T was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C16 : 0, C16 : 0 and C17 : 1ω6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol%. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5T to the genus Nocardioides . The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5T from existing species with validly published names. Therefore, strain 2C1-5T represents a novel species of the genus Nocardioides , for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5T ( = JCM 17460T = KCTC 19799T).
- Published
- 2013