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In this study, we analyzed hydroxylated polychlorinated biphenyls (OH-PCBs) in urine of both PCB transport workers and PCB researchers. A method to monitor OH-PCB in urine was developed. Urine was solid-phase extracted with 0.1% ammonia/ methanol (v/v) and glucuronic acid/sulfate conjugates and then decomposed using β-glucuronidase/arylsulfatase. After alkaline digestion/derivatization, the concentration of OH-PCBs was determined by HRGC/HRMS-SIM. In the first sampling campaign, the worker's OH-PCB levels increased several fold after the PCB waste transportation work, indicating exposure to PCBs. The concentration of OH-PCBs in PCB transport workers' urine (0.55~11 μg/g creatinine (Cre)) was higher than in PCB researchers' urine (

To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments. © 2012 The Author(s).

The aim of this study was to investigate 12 perfluorinated compounds (PFCs) including perfluorinated carboxylates (C4-C12) and perfluorinated alkyl sulfonates (C4, C6, and C8) in river and seawater samples to determine contamination levels in the aquatic environment of Hyogo prefecture, Japan. High levels of perfluorohexanoic acid (PFHxA; 2300-16,000 ng/L) were detected in the Samondogawa River at Tatsumi Bridge downstream of a PFC production facility; this location also had the highest mass flow rate of PFCs (3900-29,000 kg/y). Widespread contamination of coastal waters was confirmed with PFHxA as the dominant compound. Perfluorooctanoic acid was also prevalent in coastal waters. The concentration of PFHxA in coastal seawater and the distance from the mouth of the Samondogawa River were inversely related. This discharge of high concentrations of PFHxA from the Samondogawa River may have affected concentrations of PFCs in Osaka Bay.

Purpose The purpose of this study was to evaluate the influences of volatile organic compounds (VOCs) emissions on hazardousness and photochemical reactivity and to propose efficient VOCs abatement strategies. Methods The atmospheric concentrations of 101 components of VOCs were measured at six sites which comprehend four urban areas, a roadside area, and an industrial area in Hyogo Prefecture. VOCs influence on hazardousness and photochemical reactivity were evaluated using VOCs measured data and the index on the health effect evaluation and ozone formation potential. The substances that require emissions reduction were selected from the evaluation results. The state and characteristics of environmental pollution were considered from the concentration trends of the selected substances. Results In industrial area, n-hexadecane, styrene, vinyl chloride monomer, and 1,2-dichloroethane showed a high concentration. In roadside area, n-hexane, n-undecane, n-dodecane, tetrachloroethylene, formaldehyde, acetaldehyde, and 1-butanol showed a high concentration. The excess cancer incidences for formaldehyde exceeded 10−5 of the level of concern for carcinogenic effect at all sites. Toluene had a high percentage of ozone production. The interannual and seasonal trends of toluene concentrations were different at a regional level and the formaldehyde concentrations in summer were significantly higher than those in winter at the urban sites. Conclusions The results show the need to prepare the emission reduction plan for the major sources such as vehicle exhaust, solvent use, and industrial processes to diminish the concentration of toluene that contributes significantly to the formation of photochemical oxidant.