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Martinez-Cava, A, Hernandez-Belmonte, A, Courel-Ibanez, J, Moran-Navarro, R, Gonzalez-Badillo, JJ, and Pallares, JG. Bench press at full range of motion produces greater neuromuscular adaptations than partial executions after prolonged resistance training. J Strength Cond Res XX(X): 000-000, 2019-Training at a particular range of motion (ROM) produces specific neuromuscular adaptations. However, the effects of full and partial ROM in one of the most common upper-limb exercises such as the bench press (BP) remain controversial. In this study, 50 recreationally to highly resistance trained men were randomly assigned to 1 of 4 training groups: full bench press (BPFULL), two-thirds bench press (BP2/3), and one-third bench press (BP1/3) and control (training cessation). Experimental groups completed a 10-week velocity-based resistance training program using the same relative load (linear periodization, 60-80% 1 repetition maximum [1RM]), only differing in the ROM trained. Individual ROM for each BP variation was determined in the familiarization and subsequently replicated in every lift during training and testing sessions. Neuromuscular adaptations were evaluated by 1RM strength and mean propulsive velocity (MPV). The BPFULL group obtained the best results for the 3 BP variations (effect size [ES] = 0.52-1.96); in turn, partial BP produced smaller improvements as the ROM decreased (BP2/3: ES = 0.29-0.78; BP1/3: ES = -0.01 to 0.66). After 10-week of training cessation, the control group declined in all neuromuscular parameters (ES = 0.86-0.92) except in MPV against low loads. Based on these findings, the BPFULL stands as the most effective exercise to maximize neuromuscular improvements in recreational and well-trained athletes compared with partial ROM variations.

The mortality of septic shock remains high [Ann Intensive Care. 2017;7:19], so apart from usual therapy based on source control and antibiotics, some patients may need rescue therapies. Blood purification systems may play a role by facilitating the nonspecific removal of inflammatory mediators and microbiological toxins. There are different hemoadsorption systems, we describe in this case report the sequential use of Polymyxin B (PMX) endotoxin-adsorbing column (Toraymixin PMX-20R; Toray, Tokyo, Japan) and Cytosorb® (Cytosorbents Corp., New Jersey, USA).

Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry systems are designed for rapid and reliable microbial identification. VITEK MS PRIME is the bioMérieux's new generation instrument equipped with a continuous load-and-go sample loading system, urgent slide prioritization for critical patient samples and new internal components for faster identification. The aim of this study was to assess the performance of VITEK MS PRIME and to compare it to that of the VITEK MS system. In addition, at two sites, we performed a time-and-motion study to evaluate the efficiency of sample analysis from colony picking to slide removal from the instrument. We analyzed by VITEK MS and VITEK MS PRIME a total of 1413 isolates (1320 bacterial and 76 yeast) deriving from routine diagnostic samples that came into four laboratories in Canada, France, Italy, and Spain. VITEK MS PRIME and VITEK MS were concordant to the species and genus level for 1354/1413 (95.8%) and to the species level for 1341/1413 (94.9%). The identification and concordance rates in individual centers were largely homogenous. Overall, VITEK MS PRIME identified 1370/1413 (97.0%) of isolates compared to 1367/1413 (96.7%) identified by VITEK MS. Identification rates were consistently high for all microorganism categories. A time-and-motion study showed that the use of VITEK MS PRIME was associated with significant time saving. VITEK MS PRIME performs as well as VITEK MS and reduces the time necessary for pathogen identification. To fully optimize the laboratory process and obtain maximum efficiency, VITEK MS PRIME must be integrated into the laboratory workflow.

Anatomical-based approaches, targeting either pulmonary vein isolation (PVI) or additional extra PV regions, represent the most commonly used ablation treatments in symptomatic patients with atrial fibrillation (AF) recurrences despite antiarrhythmic drug therapy. PVI remains the main anatomical target during catheter-based AF ablation, with the aid of new technological advances as contact force monitoring to increase safety and effective radiofrequency (RF) lesions. Nowadays, cryoballoon ablation has also achieved the same level of scientific evidence in patients with paroxysmal AF undergoing PVI. In parallel, electrical isolation of extra PV targets has progressively increased, which is associated with a steady increase in complex cases undergoing ablation. Several atrial regions as the left atrial posterior wall, the vein of Marshall, the left atrial appendage, or the coronary sinus have been described in different series as locations potentially involved in AF initiation and maintenance. Targeting these regions may be challenging using conventional point-by-point RF delivery, which has opened new opportunities for coadjuvant alternatives as balloon ablation or selective ethanol injection. Although more extensive ablation may increase intraprocedural AF termination and freedom from arrhythmias during the follow-up, some of the targets to achieve such outcomes are not exempt of potential severe complications. Here, we review and discuss current anatomical approaches and the main ablation technologies to target atrial regions associated with AF initiation and maintenance. This work was supported by the European Regional Development Fund, the Spanish Ministry of Science and Innovation (SAF2016- 80324-R), and the Fundación Interhospitalaria para la Investigación Cardiovascular (FIC). The Centro Nacional de Investigaciones Cardiovasculares (CNIC) is supported by the Spanish Ministry of Science and Innovation and the Pro-CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505). Giulio La Rosa has received a fellowship grant from the joint program between the Heart Rhythm Association of the Spanish Society of Cardiology (ARC) and CNIC. Sí

Pareja-Blanco, F, Rodríguez-Rosell, D, Aagaard, P, Sánchez-Medina, L, Ribas-Serna, J, Mora-Custodio, R, Otero-Esquina, C, Yáñez-García, JM, and González-Badillo, JJ. Time course of recovery from resistance exercise with different set configurations. J Strength Cond Res 34(10): 2867-2876, 2020-This study analyzed the response to 10 resistance exercise protocols differing in the number of repetitions performed in each set (R) with respect to the maximum predicted number (P). Ten males performed 10 protocols (R(P): 6(12), 12(12), 5(10), 10(10), 4(8), 8(8), 3(6), 6(6), 2(4), and 4(4)). Three sets with 5-minute interset rests were performed in each protocol in bench press and squat. Mechanical muscle function (countermovement jump height and velocity against a 1 m·s load, V1-load) and biochemical plasma profile (testosterone, cortisol, growth hormone, prolactin, IGF-1, and creatine kinase) were assessed at several time points from 24-hour pre-exercise to 48-hour post-exercise. Protocols to failure, especially those in which the number of repetitions performed was high, resulted in larger reductions in mechanical muscle function, which remained reduced up to 48-hour post-exercise. Protocols to failure also showed greater increments in plasma growth hormone, IGF-1, prolactin, and creatine kinase concentrations. In conclusion, resistance exercise to failure resulted in greater fatigue accumulation and slower rates of neuromuscular recovery, as well as higher hormonal responses and greater muscle damage, especially when the maximal number of repetitions in the set was high.

As the population ages, their diet changes and their energy and nutrient intake tends to decrease, affecting their body composition. The objective of this study was to evaluate the association between body composition, energy intake, and macro and micronutrient intake in people over 50 who continue in work activities. 82 people, from the Guadalajara Metropolitan Area in Jalisco, Mexico, were the participants. Questionnaires were applied to collect sociodemographic, physical activity and food consumption (FCFC) data, as well as anthropometric and body composition measures. A multivariate linear regression analysis was performed to associate the percentage of body fat with caloric intake and the intake of micro and macronutrients. The results showed that high intake of energy, carbohydrates, cereals with fat, food of animal origin and oils with protein are related to a high percentage of body fat (R2 = 0.42, p-value = 0.001). From this study, it is necessary to reconsider the nutrition strategies of older people. An inadequate diet could influence their nutritional status and health. It is suggested to attend the diet for this population group.

Sanchez-Moreno, M, Cornejo-Daza, PJ, Gonzalez-Badillo, JJ, and Pareja-Blanco, F. Effects of velocity loss during body mass prone-grip pull-up training on strength and endurance performance. J Strength Cond Res 34(4): 911-917, 2020-This study aimed to analyze the effects of 2 pull-up (PU) training programs that differed in the magnitude of repetition velocity loss allowed in each set (25% velocity loss "VL25" vs. 50% velocity loss "VL50") on PU performance. Twenty-nine strength-trained men (age = 26.1 ± 6.3 years, body mass [BM] = 74.2 ± 6.4 kg, and 15.9 ± 4.9 PU repetitions to failure) were randomly assigned to 2 groups: VL25 (n = 15) or VL50 (n = 14) and followed an 8-week (16 sessions) velocity-based BM prone-grip PU training program. Mean propulsive velocity (MPV) was monitored in all repetitions. Assessments performed at pre-training and post-training included estimated 1 repetition maximum; average MPV attained with all common external loads used during pre-training and post-training testing (AVinc); peak MPV lifting one's own BM (MPVbest); maximum number of repetitions to failure lifting one's own BM (MNR); and average MPV corresponding to the same number of repetitions lifting one's own BM performed during pre-training testing (AVMNR). VL25 attained significantly greater gains than VL50 in all analyzed variables except in MNR (P < 0.05). In addition, VL25 improved significantly (P < 0.001) in all the evaluated variables while VL50 remained unchanged. In conclusion, our results suggest that once a 25% velocity loss is achieved during PU training, further repetitions did not elicit additional gains and can even blunt the improvement in strength and endurance performance.

As with other pathogens, competitive interactions between Bordetella pertussis strains drive infection risk. Vaccines are thought to perturb strain diversity through shifts in immune pressures; however, this has rarely been measured because of inadequate data and analytical tools. We used 3344 sequences from 23 countries to show that, on average, there are 28.1 transmission chains circulating within a subnational region, with the number of chains strongly associated with host population size. It took 5 to 10 years for B. pertussis to be homogeneously distributed throughout Europe, with the same time frame required for the United States. Increased fitness of pertactin-deficient strains after implementation of acellular vaccines, but reduced fitness otherwise, can explain long-term genotype dynamics. These findings highlight the role of vaccine policy in shifting local diversity of a pathogen that is responsible for 160,000 deaths annually.

Yanez-Garcia, JM, Rodriguez-Rosell, D, Mora-Custodio, R, and Gonzalez-Badillo, JJ. Changes in muscle strength, jump, and sprint performance in young elite basketball players: the impact of combined high-speed resistance training and plyometrics. J Strength Cond Res XX(X): 000-000, 2019-This study aimed to compare the effect of a combined resistance training (RT) and plyometrics on strength, sprint, and jump performance in basketball players of different ages. Thirty three elite basketball players from the same academy were categorized into 3 groups by chronological age: under-13 (U13, n = 11); under-15 (U15, n = 11); and under-17 (U17, n = 11). Players participated in a 6-week strength training program that included 2 sessions each week and consisted of full squats with low loads (45-60% 1 repetition maximum) and low volume (2-3 sets and 4-8 repetitions), jumps, and sprint exercises. All repetitions were performed at maximal intended velocity. In addition to strength training sessions, subjects performed 4 on court basketball training sessions plus 2 official matches per week. After training program, all 3 experimental groups resulted in significant improvements (p < 0.05-0.001) in maximal strength (Δ: 9.2-27.3%; effect size [ES]: 0.38-0.82), countermovement jump height (Δ: 6.6-11.6%; ES: 0.37-0.95), and sprint time in 10 and 20 m (Δ: -3.9 to -0.3%; ES: 0.09-0.69) for all experimental groups. Comparison between groups showed that training program was more effective in inducing improvements in most variables assessed for U13 compared with U15 (ES: 0.11-0.42) and U17 (ES: 0.20-0.43), whereas differences between U15 and U17 were relevant in jump and strength parameters (ES: 0.20-0.35). Therefore, these findings suggest that high-speed RT combined with plyometrics produces increments in several important variables, including strength, jump, and sprint, to yield high performance during a match in young basketball players. However, training program used seems to be generally less effective as the age of the basketball players increased.

Morán-Navarro, R, Martínez-Cava, A, Sánchez-Medina, L, Mora-Rodríguez, R, González-Badillo, JJ, and Pallarés, JG. Movement velocity as a measure of level of effort during resistance exercise. J Strength Cond Res 33(6): 1496-1504, 2019-This study analyzed whether the loss of repetition velocity during a resistance exercise set was a reliable indicator of the number of repetitions left in reserve. After the assessment of one-repetition (1RM) strength and full load-velocity relationship, 30 men were divided into 3 groups according to their 1RM strength per body mass: novice, well trained, and highly trained. On 2 separate occasions and in random order, subjects performed tests of maximal number of repetitions to failure against loads of 65, 75, and 85% 1RM in 4 exercises: bench press, full squat, prone bench pull, and shoulder press. For each exercise, and regardless of the load being used, the absolute velocities associated with stopping a set before failure, leaving a certain number of repetitions (2, 4, 6, or 8) in reserve, were very similar and showed a high reliability (coefficient of variation [CV] 4.4-8.0%). No significant differences in these stopping velocities were observed for any resistance training exercise analyzed between the novice, well trained and highly trained groups. These results indicate that by monitoring repetition velocity one can estimate with high accuracy the proximity of muscle failure and, therefore, to more objectively quantify the level of effort and fatigue being incurred during resistance training. This method emerges as a substantial improvement over the use of perceived exertion to gauge the number of repetitions left in reserve.

The choice of the optimal squatting depth for resistance training (RT) has been a matter of debate for decades and is still controversial. In this study, fifty-three resistance-trained men were randomly assigned to one of four training groups: full squat (F-SQ), parallel squat (P-SQ), half squat (H-SQ), and Control (training cessation). Experimental groups completed a 10-week velocity-based RT programme using the same relative load (linear periodization from 60% to 80% 1RM), only differing in the depth of the squat trained. The individual range of motion and spinal curvatures for each squat variation were determined in the familiarization and subsequently replicated in every lift during the training and testing sessions. Neuromuscular adaptations were evaluated by one-repetition maximum strength (1RM) and mean propulsive velocity (MPV) at each squatting depth. Functional performance was assessed by countermovement jump, 20-m sprint and Wingate tests. Physical functional disability included pain and stiffness records. F-SQ was the only group that increased 1RM and MPV in the three squat variations (ES = 0.77-2.36), and achieved the highest functional performance (ES = 0.35-0.85). P-SQ group obtained the second best results (ES = 0.15-0.56). H-SQ produced no increments in neuromuscular and functional performance (ES = -0.11-0.28) and was the only group reporting significant increases in pain, stiffness and physical functional disability (ES = 1.21-0.87). Controls declined on all tests (ES = 0.02-1.32). We recommend using F-SQ or P-SQ exercises to improve strength and functional performance in well-trained athletes. In turn, the use of H-SQ is inadvisable due to the limited performance improvements and the increments in pain and discomfort after continued training.

The aim was to develop and validate a Pseudomonas aeruginosa genotypic resistance score, based on analysis of the whole genome sequence resistome, to predict antimicrobial susceptibility phenotypes.A scoring system based on the analysis of mutation-driven resistance in 40 chromosomal genes and horizontally acquired resistance (Resfinder) was developed for ceftazidime, ceftolozane/tazobactam, meropenem, ciprofloxacin and tobramycin. Resistance genes/mutations were scored from 0 (no effect) to 1 (EUCAST clinical resistance). One hundred wild-type strains obtained from 51 different hospitals during a 2017 multicentre study were fully sequenced and analysed in order to define a catalogue of natural polymorphisms in the 40 chromosomal resistance genes. The capacity of genotypic score to predict the susceptibility phenotype was tested in 204 isolates randomly selected from the 51 hospitals (four from each hospital).The analysis of the 100 wild-type isolates yielded a catalogue of 455 natural polymorphisms in the 40 genes involved in mutational resistance. However, resistance mutations and high-risk clones (such as ST235) were also documented among a few wild-type isolates. Overall, the capacity of the genotypic score (0.5) for predicting phenotypic susceptibility (S + I in the case of meropenem) was very high (95-100%). In contrast, the capacity of the genotypic score to predict resistance (≥1) was far more variable depending on the agent. Prediction of meropenem clinical resistance was particularly low (18/39, 46.1%), whereas it classified clinical ceftolozane/tazobactam resistance in 100% (7/7) of cases.Although a margin for improvement was evidenced in this proof of concept study, an overall good correlation between the genotypic resistance score and the susceptibility profile was documented. Further refining of the scoring system, automatization and testing of large international cohorts should follow.

Rodriguez-Rosell, D, Yanez-Garcia, JM, Mora-Custodio, R, Torres-Torrelo, J, Ribas-Serna, J, and Gonzalez-Badillo, JJ. Role of the effort index in predicting neuromuscular fatigue during resistance exercises. J Strength Cond Res XX(X): 000-000, 2020-This study aimed: (a) to analyze the acute mechanical, metabolic, and electromyographic (EMG) response to 16 resistance exercise protocols (REPs) defined by the first repetition's mean velocity and the percentage of velocity loss (%VL) over the set in the full-squat (SQ) exercise; and (b) to assess whether the effort index (EI, the product of the first repetition's mean velocity and the %VL in the set) could be used as an objective indicator of neuromuscular fatigue. Eleven resistance-trained men performed 16 REPs in the SQ exercise. For the configuration of the 16 REPs, 4 relative intensities (50, 60, 70, and 80% 1 repetition maximum) and 4 magnitudes of %VL (∼10, ∼20, ∼30, and ∼45%) were used. The induced fatigue after each REP was quantified using the percentage of change in (a) countermovement jump (CMJ) height, (b) mean propulsive velocity attained with the load that elicited an ∼1.00 m·s (V1 m·s load), and (c) changes in surface EMG variables. Blood lactate concentration was also collected. The EI presented very strong relationships with the %VL with the V1 m·s load, CMJ height, and post-exercise lactate concentration (r = 0.92, 0.93, and 0.91, respectively; p < 0.001). Moderate to strong relationships were found between the EI and the changes in amplitude (r = 054-0.58; p < 0.05), frequency (r = -0.55 to -0.83; p < 0.05-0.001), and time-frequency (r = 0.52-0.77; p < 0.05-0.001) EMG variables. In addition, the moderate to strong relationships found between the relative changes in mechanical, metabolic, and EMG variables after each REP reinforce the validity of the EI as an objective indicator of muscle fatigue, metabolic stress, and neural effect induced by typical resistance training sessions. Thus, by adjusting the EI in each session, it is possible to quantifying the actual level of effort experienced by each individual during resistance exercises.

This study aimed to compare the effects of 2 resistance training (RT) programs with different velocity losses (VLs) allowed in each set: 10% (VL10%) versus 30% (VL30%) on neuromuscular performance and hormonal response. Twenty-five young healthy males were randomly assigned into 2 groups: VL10% (n = 12) or VL30% (n = 13). Subjects followed a velocity-based RT program for 8 weeks (2 sessions per week) using only the full-squat (SQ) exercise at 70%–85% 1-repetition maximum (1RM). Repetition velocity was recorded in all training sessions. A 20-m running sprint, countermovement jump (CMJ), 1RM, muscle endurance, and electromyogram (EMG) during SQ exercise and resting hormonal concentrations were assessed before and after the RT program. Both groups showed similar improvements in muscle strength and endurance variables (VL10%: 7.0%–74.8%; VL30%: 4.2%–73.2%). The VL10% resulted in greater percentage increments in CMJ (9.2% vs. 5.4%) and sprint performance (–1.5% vs. 0.4%) than VL30%, despite VL10% performing less than half of the repetitions than VL30% during RT. In addition, only VL10% showed slight increments in EMG variables, whereas no significant changes in resting hormonal concentrations were observed. Therefore, our results suggest that velocity losses in the set as low as 10% are enough to achieve significant improvements in neuromuscular performance, which means greater efficiency during RT. Novelty The VL10% group showed similar or even greater percentage of changes in physical performance compared with VL30%. No significant changes in resting hormonal concentrations were observed for any training group. Curvilinear relationships between percentage VL in the set and changes in strength and CMJ performance were observed.

The aim of this article is to present a case of mycotic aneurysm of internal carotid artery secondary to livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) treated with resection and common-to-internal carotid artery bypass with autologous vein graft in a male pig farmer. A 69-year-old man, pig farmer, with recent dental extraction was admitted with a right cervical pulsatile mass, dysphonia, pain, leukocytosis and elevated C-reactive protein (CRP). Ultrasonography (US) and computed tomography angiography (CTA) showed a 3.9 × 4.5 cm mycotic aneurysm of right internal carotid artery with hypermetabolic uptake in positron emission tomography (PET) scan. Resection of the mycotic aneurysm and a common-to-internal carotid artery bypass with major saphenous vein graft were performed. LA-MRSA clonal complex (CC) 398 was detected in intraoperative samples and antibiotic therapy was changed according to antibiogram. Patient was discharged at the seventh postoperative day and received antibiotic therapy for 6 weeks. US 12 months later showed patency of the bypass without collections. Mycotic aneurysms of internal carotid artery are very infrequent. MRSA isolation is rare, and to the best of our knowledge this is the first case caused by multi-drug resistant LA-MRSA CC398. The treatment includes mycotic aneurysm resection and reconstruction with venous graft bypass plus intensive antibiotic therapy.

Background In high-income countries, shigellosis is mainly found in travellers to high-risk regions or in men who have sex with men (MSM). This study investigated the genomic characteristics and the features of antimicrobial resistance of MSM-associated Shigella flexneri and Shigella sonnei circulating in Barcelona, Spain, elucidating their connectivity with contemporaneous Shigella spp. from other countries. Methods Antimicrobial susceptibility, whole-genome sequencing, genomic characterization and phylogenetic analysis were performed in MSM-associated Shigella spp. recovered from 2015 to 2019. Reference genomes of MSM-associated Shigella spp. were included for contextualization and to determine their connection with international outbreaks. Results In total, 44 S. flexneri and 26 S. sonnei were identified among MSM. Overall, 80% showed resistance to azithromycin, 65.7% showed resistance to trimethoprim-sulphamethoxazole and 32.8% showed resistance to ciprofloxacin; 27.1% were resistant to all three antimicrobials. mphA and/or ermB, and qnrS and mutations in the quinolone resistance determining regions were found in the azithromycin- and ciprofloxacin-resistant isolates, respectively. Additionally, two isolates carried blaCTX-M-27. Single-nucleotide-polymorphism-based analysis revealed that the isolates were organized into different lineages, most of which were closely related to dominant MSM-associated lineages described previously in the UK and Australia. Conclusions This study investigated the circulation of lineages of S. flexneri and S. sonnei among MSM in Spain that were mainly resistant to first-/second-line oral treatments, and closely related to dominant MSM-associated lineages described previously in the UK and Australia. These data reinforce the urgent need for the implementation of public health measures focusing on the early detection and prevention of transmission of this emerging pathogen, which is contributing to the antimicrobial resistance crisis in sexually transmitted infections.

Rate of force development (RFD) refers to the ability of the neuromuscular system to increase contractile force from a low or resting level when muscle activation is performed as quickly as possible, and it is considered an important muscle strength parameter, especially for athletes in sports requiring high-speed actions. The assessment of RFD has been used for strength diagnosis, to monitor the effects of training interventions in both healthy populations and patients, discriminate high-level athletes from those of lower levels, evaluate the impairment in mechanical muscle function after acute bouts of eccentric muscle actions and estimate the degree of fatigue and recovery after acute exhausting exercise. Notably, the evaluation of RFD in human skeletal muscle is a complex task as influenced by numerous distinct methodological factors including mode of contraction, type of instruction, method used to quantify RFD, devices used for force/torque recording and ambient temperature. Another important aspect is our limited understanding of the mechanisms underpinning rapid muscle force production. Therefore, this review is primarily focused on (i) describing the main mechanical characteristics of RFD; (ii) analysing various physiological factors that influence RFD; and (iii) presenting and discussing central biomechanical and methodological factors affecting the measurement of RFD. The intention of this review is to provide more methodological and analytical coherency on the RFD concept, which may aid to clarify the thinking of coaches and sports scientists in this area.

To describe the acute and delayed time course of recovery following resistance training (RT) protocols differing in the number of repetitions (R) performed in each set (S) out of the maximum possible number (P). Ten resistance-trained men undertook three RT protocols [S × R(P)]: (1) 3 × 5(10), (2) 6 × 5(10), and (3) 3 × 10(10) in the bench press (BP) and full squat (SQ) exercises. Selected mechanical and biochemical variables were assessed at seven time points (from − 12 h to + 72 h post-exercise). Countermovement jump height (CMJ) and movement velocity against the load that elicited a 1 m s−1 mean propulsive velocity (V1) and 75% 1RM in the BP and SQ were used as mechanical indicators of neuromuscular performance. Training to muscle failure in each set [3 × 10(10)], even when compared to completing the same total exercise volume [6 × 5(10)], resulted in a significantly higher acute decline of CMJ and velocity against the V1 and 75% 1RM loads in both BP and SQ. In contrast, recovery from the 3 × 5(10) and 6 × 5(10) protocols was significantly faster between 24 and 48 h post-exercise compared to 3 × 10(10). Markers of acute (ammonia, growth hormone) and delayed (creatine kinase) fatigue showed a markedly different course of recovery between protocols, suggesting that training to failure slows down recovery up to 24–48 h post-exercise. RT leading to failure considerably increases the time needed for the recovery of neuromuscular function and metabolic and hormonal homeostasis. Avoiding failure would allow athletes to be in a better neuromuscular condition to undertake a new training session or competition in a shorter period of time.

The landscape is a complex concept that results from the interplay of nature, society and culture. It`s outlined, firstly, the geographical ideas, general concepts, perception and interpretation of Spanish landscape. For the first time, a new word is proposed for the Spanish language: “maresaje”, to make reference to the marine landscape or seascape. Therefore, this paper aims to highlight the interest of deep and valuable images about landscape and seascape coming from literature and painting for the construction of Spanish cultural identity.

Rodriguez-Rosell, D, Franco-Marquez, F, Mora-Custodio, R, and Gonzalez-Badillo, JJ. Effect of high-speed strength training on physical performance in young soccer players of different ages. J Strength Cond Res 31(9): 2498-2508, 2017-The aim of the present study was to compare the effectiveness of low-load, low-volume weight training combined with plyometrics on strength, sprint, and jump performance in soccer players of different ages. Eighty-six soccer players from the same academy were categorized into 3 groups by age (under 13 years, U13, n = 30; under 15, U15, n = 28; and under 17, U17, n = 28) and then randomly assigned into 2 subgroups: a strength training group (STG) and a control group (CG). The strength training program was performed twice a week for 6 weeks and consisted of full squats (load: 45-60% 1 repetition maximum; volume: 3 set of 8-4 repetitions), jumps, and straight line sprint exercises. After training intervention, the STGs showed significant improvements in maximal strength (7.5-54.5%; p < 0.001), jump height (5.7-12.5%; p

Rodríguez-Rosell, D, Mora-Custodio, R, Franco-Márquez, F, Yáñez-García, JM, González-Badillo, JJ. Traditional vs. sport-specific vertical jump tests: reliability, validity, and relationship with the legs strength and sprint performance in adult and teen soccer and basketball players. J Strength Cond Res 31(1): 196-206, 2017-The vertical jump is considered an essential motor skill in many team sports. Many protocols have been used to assess vertical jump ability. However, controversy regarding test selection still exists based on the reliability and specificity of the tests. The main aim of this study was to analyze the reliability and validity of 2 standardized (countermovement jump [CMJ] and Abalakov jump [AJ]) and 2 sport-specific (run-up with 2 [2-LEGS] or 1 leg [1-LEG] take-off jump) vertical jump tests, and their usefulness as predictors of sprint and strength performance for soccer (n = 127) and basketball (n = 59) players in 3 different categories (Under-15, Under-18, and Adults). Three attempts for each of the 4 jump tests were recorded. Twenty-meter sprint time and estimated 1 repetition maximum in full squat were also evaluated. All jump tests showed high intraclass correlation coefficients (0.969-0.995) and low coefficients of variation (1.54-4.82%), although 1-LEG was the jump test with the lowest absolute and relative reliability. All selected jump tests were significantly correlated (r = 0.580-0.983). Factor analysis resulted in the extraction of one principal component, which explained 82.90-95.79% of the variance of all jump tests. The 1-LEG test showed the lowest associations with sprint and strength performance. The results of this study suggest that CMJ and AJ are the most reliable tests for the estimation of explosive force in soccer and basketball players in different age categories.

Rodriguez-Rosell, D, Yanez-Garcia, JM, Sanchez-Medina, L, Mora-Custodio, R, and Gonzalez-Badillo, JJ. Relationship between velocity loss and repetitions in reserve in the bench press and back squat exercises. J Strength Cond Res 34(9): 2537-2547, 2020-This study aimed to compare the pattern of repetition velocity decline during a single set to failure performed against 4 relative loads in the bench press (BP) and full back squat (SQ) exercises. After an initial test to determine 1 repetition maximum (1RM) strength and load-velocity relationships, 20 men performed one set of repetitions to failure (MNR test) against loads of 50, 60, 70, and 80% 1RM in BP and SQ, on 8 random order sessions performed every 6-7 days. Velocity against the load that elicited a ∼1.00 m·s (V1 m·s load) was measured before and immediately after each MNR test, and it was considered a measure of acute muscle fatigue. The number of repetitions completed against each relative load showed high interindividual variability in both BP (coefficient of variation [CV]: 15-22%) and SQ (CV: 26-34%). Strong relationships were found between the relative loss of velocity in the set and the percentage of performed repetitions in both exercises (R = 0.97 and 0.93 for BP and SQ, respectively). Equations to predict repetitions left in reserve from velocity loss are provided. For a given magnitude of velocity loss within the set (15-65%), the percentages of performed repetitions were lower for the BP compared with the SQ for all loads analyzed. Acute fatigue after each set to failure was found dependent on the magnitude of velocity loss (r = 0.97 and 0.99 for BP and SQ, respectively) but independent of the number of repetitions completed by each participant (p > 0.05) for both exercises. The percentage of velocity loss against the V1 m·s load decreased as relative load increased, being greater for BP than SQ. These findings indicate that monitoring repetition velocity can be used to provide a very good estimate of the number (or percentage) of repetitions actually performed and those left in reserve in each exercise set, and thus to more objectively quantify the level of effort incurred during resistance training.

Bachero-Mena, B, Pareja-Blanco, F, and González-Badillo, JJ. Effects of resistance training on physical performance in high-level 800-meter athletes: a comparison between high-speed resistance training and circuit training. J Strength Cond Res 35(7): 1905-1915, 2021-This study compared the effects of 2 resistance training programs during 25 weeks on physical performance and hormonal response in high-level 800 m athletes. Thirteen male athletes (800-m personal best: 1:43-1:58 minutes:ss) were divided into 2 groups: high-speed resistance training group (RTG) (n = 6) and circuit training group (CTG) (n = 7). Three tests (T1, T2, and T3) including sprint and 800 m running, strength exercises, and blood hormones samples were performed. Both groups showed improvements in 800 m performance (RTG: likely positive, 80/20/0%; CTG: very likely positive, 98/2/0%); however, RTG showed an additional improvement in 200 m (likely positive, 85/15/0%), countermovement jump (CMJ) (very likely positive, 98/2/0%), and squat (likely positive, 91/9/0%), whereas CTG reached likely positive (88/11/1%) effects in CMJ and unclear/possibly negative effects in the rest of the strength variables analyzed. Concerning hormones, RTG resulted in a likely increase (83/15/3%) in testosterone from T1 to T3, and CTG showed a likely increase (79/17/4%) in cortisol from T2 to T3, remaining the rest of the hormones analyzed unclear. These results suggest that a resistance training characterized by high-speed and low-volume produced better improvements in both strength and running performance than a circuit training, accompanied by little changes in the hormonal response.

Introduction and objectives: We describe the results for Spain of the Second European Cardiac Resynchronization Therapy Survey (CRT-Survey II) and compare them with those of the other participating countries. Methods: We included patients undergoing CRT device implantation between October 2015 and December 2016 in 36 participating Spanish centers. We registered the patients' baseline characteristics, implant procedure data, and short-term follow-up information until hospital discharge. Results: Implant success was achieved in 95.9%. The median [interquartile range] annual implantation rate by center was significantly lower in Spain than in the other participating countries: 30 implants/y [21-50] vs 55 implants/y [33-100]; P = .00003. In Spanish centers, there was a lower proportion of patients >= 75 years (27.9% vs 32.4%; P = .0071), a higher proportion in New York Heart Association functional class II (46.9% vs 36.9%; P < .00001), and a higher percentage with electrocardiographic criteria of left bundle branch block (82.9% vs 74.6%; P < .00001). The mean length of hospital stay was significantly lower in Spanish centers (5.8 +/- 8.5 days vs 6.4 +/- 11.6; P < .00001). Spanish patients were more likely to receive a quadripolar LV lead (74% vs 56%; P < .00001) and to be followed up by remote monitoring (55.8% vs 27.7%; P = 75 years received a CRT device, while more patients were in New York Heart Association functional class II and had left bundle branch block. In addition, the length of hospital stay was shorter, and there was greater use of quadripolar LV leads and remote CRT monitoring. (C) 2018 Sociedad Espanola de Cardiologia. Published by Elsevier Espana, S.L.U. All rights reserved.

Strength and conditioning specialists commonly deal with the quantification and selection the setting of protocols regarding resistance training intensities. Although the one repetition maximum (1RM) method has been widely used to prescribe exercise intensity, the velocity-based training (VBT) method may enable a more optimal tool for better monitoring and planning of resistance training (RT) programs. The aim of this study was to compare the effects of two RT programs only differing in the training load prescription strategy (adjusting or not daily via VBT) with loads from 50 to 80% 1RM on 1RM, countermovement (CMJ) and sprint. Twenty-four male students with previous experience in RT were randomly assigned to two groups: adjusted loads (AL) (n = 13) and non-adjusted loads (NAL) (n = 11) and carried out an 8-week (16 sessions) RT program. The performance assessment pre- and post-training program included estimated 1RM and full load-velocity profile in the squat exercise; countermovement jump (CMJ); and 20-m sprint (T20). Relative intensity (RI) and mean propulsive velocity attained during each training session (Vsession) was monitored. Subjects in the NAL group trained at a significantly faster Vsession than those in AL (p < 0.001) (0.88–0.91 vs. 0.67–0.68 m/s, with a ∼15% RM gap between groups for the last sessions), and did not achieve the maximum programmed intensity (80% RM). Significant differences were detected in sessions 3–4, showing differences between programmed and performed Vsession and lower RI and velocity loss (VL) for the NAL compared to the AL group (p < 0.05). Although both groups improved 1RM, CMJ and T20, NAL experienced greater and significant changes than AL (28.90 vs.12.70%, 16.10 vs. 7.90% and −1.99 vs. −0.95%, respectively). Load adjustment based on movement velocity is a useful way to control for highly individualised responses to training and improve the implementation of RT programs.

This study presents an effective technology for the olive processing industry to remediate olive washing water. A 14.5-L enclosed tubular photobioreactor was inoculated with a stable microalgal-bacterial consortium obtained by screening strains well adapted to olive washing water. The capacity of an enclosed tubular photobioreactor to remove toxic compounds was evaluated under photosynthesis conditions and without any external supply of oxygen. The results showed that the dominant green microalgae Scenedesmus obliquus, Chlorella vulgaris and the cyanobacteria Anabaena sp. and bacteria present in olive washing water (i.e. Pantoea agglomerans and Raoultella terrigena) formed a synergistic association that was resistant to toxic pollutants present in the effluent and during the initial biodegradation process, which resulted in the breakdown of the pollutant. Total phenolic compounds, COD, BOD5, turbidity and colour removals of 90.3 ± 11.4, 80.7 ± 9.7, 97.8 ± 12.7, 82.9 ± 8.4 and 83.3 ± 10.4 %, respectively, were recorded in the photobioreactor at 3 days of hydraulic retention time.

It is generally accepted that preadolescents have a limited capacity to develop muscle hypertrophy in response to exercise compared with older populations; however, studies are scarce and conflicting. The main aim of the present study was to assess if playing tennis is associated with the hypertrophy of dominant pectoralis muscles (PM) in professional (PRO) and in prepubescent tennis players (PRE). A secondary aim was to assess if the degree of asymmetry of PM is greater in PRO than PRE. The volume of PM of both sides was determined using magnetic resonance imaging in 8 male PRO (21.9 years), 6 male PRE (11 years, Tanner 1-2) and 12 male non-active controls (6 adults: 23.5 years; and 6 prepubescents: 10.7 years, Tanner 1-2). PRO and PRE had 15 and 30% greater volume, respectively, in the dominant than in the contralateral PM (P

En el presente trabajo se aborda una problemática de larga trayectoria en el empleo público (el abuso del empleo temporal) que se ha agudizado en los últimos tiempos como consecuencia de la crisis económica. Concretamente se examinan las diversas respuestas que se han ofrecido tanto por las entidades públicas empleadoras como por los Juzgados y Tribunales de los órdenes jurisdiccionales que conocen de las pretensiones formuladas por las dos grandes categorías de personal al servicio de las Administraciones Públicas (funcionarios y trabajadores en régimen laboral). Finalmente se realiza un análisis crítico de las principales fórmulas articuladas como reacción a la problemática expuesta, con opción razonada por aquella que se considera más adecuada.This paper deals with a long-standing problem in public employment (the abuse of temporary employment) that has wors-ened in recent times as a result of the economic crisis. Specifically, it examines the various responses that have been offered both by public employers and by the Courts and Tribunals of jurisdictional orders that hear the claims made by the two large categories of personnel at the service of Public Administrations (officials and workers in labor regime). Finally, a critical analysis of the main formulas articulated as a reaction to the exposed problem is made, with a reasoned option for the one considered most appropriate.

We describe two cystic fibrosis patients infected with pandrug-resistant Burkholderia cepacia complex, with the exception of ceftazidime–avibactam, who received prophylaxis with this antibiotic during lung transplantation. Although both patients had a post-operative relapse of respiratory infection, one with positive blood cultures, ceftazidime–avibactam treatment yielded a favourable outcome. 12 months after transplantation, one patient presented an excellent clinical outcome. However, the other patient died 10 months later due to severe B. cepacia sinusitis with intracranial invasion.

This study aimed to evaluate the effectiveness and safety of teicoplanin for treating enterococcal infective endocarditis (EIE). A retrospective analysis of a prospective cohort of definite EIE patients treated with teicoplanin in a Spanish referral centre (2000-2017) was performed. The primary outcome was mortality during treatment. Secondary outcomes were mortality during 3-month follow-up, adverse effects and relapse. A total of 22 patients received teicoplanin, 9 (40.9%) as first-line (8 Enterococcus faecium and 1 Enterococcus faecalis) and 13 (59.1%) as salvage therapy (13 E. faecalis). Median (IQR) age was 71.5 (58.3-78) years and Charlson comorbidity index was 4.5 (3-7). Five (22.7%) affected prosthetic valves. Median duration of treatment in survivors was 53 (42.5-61) days for antibiotics and 27 (17-41.5) days for teicoplanin [median dose 10 (10-10.8) mg/kg/day]. Reasons for teicoplanin use were resistance to β-lactams (40.9%), adverse events with previous regimens (31.8%) and outpatient parenteral antimicrobial therapy (OPAT) (27.3%). Teicoplanin was withdrawn due to adverse events in 2 patients (9.1%). Five patients (22.7%) died during treatment: four in the first-line (three with surgery indicated but not performed) and one in the salvage therapy group (surgery indicated but not performed). Two deaths (11.8%) occurred over the 3-month follow-up. There were no relapses during a median of 43.2 (22.1-69.1) months. Teicoplanin can be used as an alternative treatment for susceptible E. faecium IE and as a salvage therapy in selected patients with E. faecalis IE when adverse events develop with standard regimens or to allow OPAT.

The abundance of fungi in a full-scale membrane bioreactor (MBR) treating urban wastewater and experiencing seasonal foaming was assessed by quantitative PCR (qPCR), comparing three different sets of widely used universal fungal primers targeting the gene encoding the small ribosomal subunit RNA, 18S-rDNA, (primers NS1-Fung and FungiQuant) or the internal transcribed spacer ITS2 (primers ITS3-ITS4). Fungi were a numerically important fraction of the MBR microbiota (≥106 18S-rDNA copies/L activated sludge), and occurred both in the aerated and anoxic bioreactors. The numbers of copies of fungal markers/L activated sludge calculated using the NS1-Fung or ITS3-ITS4 primer sets were up to 2 orders of magnitude higher than the quantifications based on the FungiQuant primers. Fungal 18S-rDNA counts derived from the FungiQuant primers decreased significantly during cold seasons, concurring with foaming episodes in the MBR. Redundancy analysis corroborated that temperature was the main factor driving fungi abundance, which was also favored by longer solid retention time (SRT), lower chemical oxygen demand/biochemical oxygen demand at 5 days (COD/BOD5) of influent water, and lower biomass accumulation in the MBR.

Phage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated from faeces. Nine ARGs (bla(TEM), bla(CTX-M-1 group), bla(CTX-M-9 group), bla(OXA-48), qnrA, qnrS, mecA, sul1 and armA) were quantified by qPCR in the phage DNA fractions of 150 faecal samples obtained from healthy individuals who had not received antibiotic treatment or travelled abroad in the 3 months prior to sample collection. On the suspicion that the detected particles originated from bacterial flora, 82 Escherichia coli and Klebsiella pneumoniae isolates possessing at least one identified ARG (bla(TEM), bla(CTX-M-1 group), bla(CTX-M-9 group), armA, qnrA, qnrS and sul1) were isolated and their capacity to produce phage particles carrying these ARGs following induction was evaluated. Of 150 samples, 72.7% were positive for at least one ARG, with bla(TEM) and bla(CTX-M-9 group) being the most prevalent and abundant. Of the 82 isolates, 51 (62%) showed an increase in the number of copies of the respective ARG in the phage fraction following induction, with bla(TEM), bla(CTX-M-1 group), bla(CTX-M-9 group) and sul1 being the most abundant. Phages induced from the isolates were further purified and visualised using microscopy and their DNA showed ARG levels of up to 1010 gene copies/mL. This study highlights the abundance of phage particles harbouring ARGs and indicates that bacterial strains in the intestinal habitat could be source of these particles. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

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The activity of eight aminoglycosides (amikacin, apramycin, arbekacin, gentamicin, kanamycin, neomycin, netilmicin and tobramycin) against a collection of 257 amoxicillin/clavulanic acid (AMC)-resistant Escherichia coli isolates was determined by microdilution. Aminoglycoside resistance rates, the prevalence of aminoglycoside-modifying enzyme (AME) genes, the relationship between AME gene detection and resistance phenotype to aminoglycosides, and the association of AME genes with mechanisms of AMC resistance in E. coli isolates in Spain were investigated. Aminoglycoside-resistant isolates were screened for the presence of genes encoding common AMEs [aac(3)-Ia, aac(3)-IIa, aac(3)-IVa, aac(6')-Ib, ant(2")-Ia, ant(4')-IIa and aph(3')-Ia] or 16S rRNA methylases (armA, rmtB, rmtC and npmA). In total, 105 isolates (40.9%) were resistant to at least one of the aminoglycosides tested. Amikacin, apramycin and arbekacin showed better activity, with MIC90 values of 2 mg/L (arbekacin) and 8 mg/L (amikacin and apramycin). Kanamycin presented the highest MIC90 (128 mg/L). The most common AME gene was aac(6')-Ib (36 strains; 34.3%), followed by aph(3')-Ia (31 strains; 29.5%), ant(2")-Ia (29 strains; 27.6%) and aac(3)-IIa (23 strains; 21.9%). aac(3)-Ia, aac(3)-IVa, ant(4')-IIa and the four methylases were not detected. The ant(2")-Ia gene was usually associated with OXA-1 [21/30; 70%], whilst 23/25 (92%) strains producing CTX-M-15 had the aac(6')-Ib gene. The most prevalent AME gene was aac(6')-Ib (18/41; 44%) in nosocomial isolates, whilst ant(2")-Ia and aph(3')-Ia genes (20/64; 31%) were more frequent in strains of community origin. In 64.6% isolates the phenotypic profile correlated with the presence of commonly encountered AMEs. (C) 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

The community structure and population dynamics of Mycolata were monitored in a full-scale membrane bioreactor during four experimental phases under changing operating and environmental conditions, by means of temperature-gradient gel electrophoresis of partial 16S-rRNA genes amplified from community DNA and RNA templates (total and active populations). Non-metric multidimensional scaling and BIO-ENV analyses demonstrated that population dynamics were mostly explained (30-32%) by changes in the input of nutrients in the influent water and the accumulation of biomass in the bioreactors, while the influence of hydraulic and solid retention times, temperature and F/M ratio was minor. Significant correlations were observed between particular Mycolata phylotypes and one or more variables, contributing information for the prediction of their abundance and activity under changing conditions. Fingerprinting and multivariate analyses demonstrated that two foaming episodes, recorded at temperatures20°C, were connected to the increase of the relative abundance of Mycolata unrelated to Gordonia amarae.

Ammonia-oxidizing bacteria (AOB), ammonia oxidizing archaea (AOA) and N2O-reducing denitrifiers were measured by quantitative real-time PCR (qPCR) in activated sludge samples from four full-scale wastewater treatment plants (WWTPs) in South Spain, and their abundances were linked to the generation of N2O in the samples using multivariate analysis (Non-metric multidimensional scaling, MDS, and BIO-ENV). The average abundances of AOA remained in similar orders of magnitude in all WWTPs (106 copies amoA/L activated sludge mixed liquor), while significant differences were detected for AOB (105-109copies amoA/L) and N2O-reducers (107-1010copies nosZ/L). Average N2O emissions measured in activated sludge samples ranged from 0.10 ± 0.05 to 6.49 ± 8.89 mg N2O-N/h/L activated sludge, and were strongly correlated with increased abundances of AOB and lower counts of N2O-reducers. A significant contribution of AOA to N2O generation was unlikely, since their abundance correlated negatively to N2O emissions. AOB abundance was favoured by higher NO3- and NO2-concentrations in the activated sludge.

Ortega-Becerra, M, Pareja-Blanco, F, Jimenez-Reyes, P, Cuadrado-Penafiel, V, and Gonzalez-Badillo, JJ. Determinant factors of physical performance and specific throwing in handball players of different ages. J Strength Cond Res 32(6): 1778-1786, 2018-This study aimed to analyze various fitness qualities in handball players of different ages and to determine the relationships between these parameters and throwing velocity. A total of 44 handball players participated, pooled by age groups: professional (ELITE, n = 13); under-18 (U18, n = 16); under-16 (U16, n = 15). The following tests were completed: 20-m running sprints; countermovement jumps (CMJs); jump squat to determine the load that elicited ∼20 cm jump height (JSLOAD-20 cm); a progressive loading test in full squat and bench press to determine the load that elicited ∼1 m·s (SQ-V1-LOAD and BP-V1-LOAD); and handball throwing (jump throw and 3-step throw). ELITE showed greater performance in almost all sprint distances, CMJ, JSLOAD-20 cm, and bench press strength than U18 and U16. The differences between U18 and U16 were unclear for these variables. ELITE also showed greater (p < 0.001) performance for squat strength and throwing than U18 and U16, and U18 attained greater performance (p ≤ 0.05) for these variables than U16. Throwing performance correlated (p ≤ 0.05) with sprint times (r = -0.31; -0.51) and jump ability (CMJ: r = 0.39; 0.56 and JSLOAD-20 cm: r = 0.57; 0.60). Muscle strength was also associated (p < 0.001) with both types of throw (SQ-V1-LOAD: r = 0.66; 0.76; and BP-V1-LOAD: r = 0.33; 0.70). These results indicate that handball throwing velocity is strongly associated with lower-limb strength, although upper-limb strength, jumping and sprint capacities also play a relevant role in throwing performance, suggesting the need for coaches to include proper strength programs to improve handball players' throwing velocity.

In this study, the neural networks are used to predict and explain the behavior of different edaphological variables in the adsorption and retention of heavy metals, both isolated and competing. A comparison with the results obtained using multiple regression, stepwise analysis, and regression trees is performed. In the neural network technique, CEC amorphous and crystallized oxides and kaolinite in the clay fraction are the most selected variables for making the optimal models, while mica and, to a lesser extent, plagioclase, are the next variables selected. Additionally, a competitive model has been considered, using simultaneously different metals. In the competitive model, the model predicts a more intense competence between Pb and Ni for the adsorption process and between Cr and Ni for the retention process.

Improvements in early detection and treatment have markedly reduced cancer-related mortality. However survival not only depends on effectively cure cancer, but prevention, diagnosis and treatment of cancer-related complications is also needed. Cardiovascular toxicity is a widespread problem across many classes of therapeutic schemes, however scientific evidence in the management of cardiovascular complications of onco-hematological patients is scarce, as these patients have been systematically excluded from clinical trials and current recommendations are based on expert consensus. Multidisciplinary teams are mandatory to decrease morbidity and mortality from both cardiotoxicity and cancer itself. An excessive concern for the occurrence of cardiovascular toxicity, can avoid potentially curative therapies, while underestimating this risk, increases long-term mortality of cancer survivors. The objective of this consensus document, developed in collaboration of the Spanish Society of Cardiology, the Spanish Society of Medical Oncology, the Spanish Society of Radiation Oncology and the Spanish Society of Hematology, is to update the necessary concepts and expertise on cardio-onco-hematology that enable its application in daily clinical practice and to promote the development of local multidisciplinary teams, to improve the cardiovascular health of patients with cancer.

Long-term dynamics of total and active bacterial populations in a full-scale membrane bioreactor (MBR) treating urban wastewater were monitored during nine months by temperature-gradient gel electrophoresis (TGGE) of partial 16S-rRNA genes, amplified from community DNA and RNA templates. The bacterial community, dominated by Alphaproteobacteria, displayed the required characteristics for a successful and steady contaminant removal under real operating conditions. The evolution of population dynamics showed that a fully-stable microbial community was not developed even after technical stabilization and steady performance of the MBR were achieved. Non-metric multidimensional scaling and BIO-ENV demonstrated that the trends of the populations were often mostly explained by temperature, followed by the concentration of volatile suspended solids and C/N ratio of the influent. These variables were mainly responsible for triggering the shifts between functionally redundant populations. These conclusions contribute to the prediction of the complex profiles of adaptation and response of bacterial populations under changing conditions.

INTRODUCCION: La Tempestad Electrica (TE) se refiere a un estado de inestabilidad electrica cardiaca caracterizada por multiples episodios de taquicardia ventricular dentro de un periodo relativamente corto de tiempo. La mayoria de las arritmias que se desarrollan durante una TE son la Taquicardia Ventricular (TV) del tipo de Torsades de Pointes (TdP), TV polimorfas y excepcionalmente Fibrilacion Ventricular. Debido a su muy rara presentacion y mas aun su registro, se presenta este caso. CASO CLINICO: Se presenta, a continuacion el primer caso documentado a nivel nacional de Tempestad Electrica sostenida por mas de 36 horas asociada a un sindrome de Q-T largo congenito en una paciente de 26 anos de edad. Se realizo manejo farmacologico concomitantemente con tratamiento extremo por medio de implante de marcapaso en vigencia de TE. CONCLUSION. La tempestad electrica es una emergencia medica, de rara presentacion que obliga a tomar decisiones rapidas y acertadas para evitar un potencial desenlace fatal.

This work describes the removal of 6 quinolone antibiotics from wastewaters under different redox conditions (aerobic, nitrifying and anoxic) through batch experiments in laboratory scale activated sludge reactors using mixed liquor from a membrane bioreactor pilot plant (MBR). The main removal pathways for antibiotics from wastewaters involved in each treatment are described. Mass balances indicated that sorption on sludge played a dominating role in the elimination of antibiotics. Sorption potential depended on the redox conditions, being lower in nitrifying (Kd, 414-876 L kg(-1)) and anoxic (Kd, 471-930 L kg(-1)) sludge in comparison with aerobic sludge (Kd, 534-1137 L kg(-1)). Kd was higher for piperazinylic quinolones. Redox conditions also influenced biodegradation, a secondary pathway, which followed first-order kinetics with degradation rates constants ranging from 1.8·10(-3) to 8.2·10(-3) h(-1). Biodegradation rates under anoxic conditions were negligible. The experimental results have also demonstrated much higher removal efficiency by biodegradation (36.2-60.0%) under nitrifying conditions in comparison with aerobic conditions (14.9-43.8%). The addition of allylthiourea, an ammonia monooxygenase inhibitor, inhibited nitrification completely and reduced significantly the biodegradation of target antibiotics (16.5-29.3%). The residual biodegradation in the presence of allylthiourea may be due to the activity of heterotrophs in the enriched nitrifier culture. The removal of the selected antibiotics under the studied redox conditions depended significantly on the bacteria composition of the sludge. These results suggest that despite the known persistence of this group of antibiotics it is possible to enhance their degradation using nitrifying conditions, which at adequate working conditions as high SRT, typical in MBR, become a promising alternative for improving quinolones removal from environment.

Olive breeding programmes are focused on selecting for traits as short juvenile period, plant architecture suited for mechanical harvest, or oil characteristics, including fatty acid composition, phenolic, and volatile compounds to suit new markets. Understanding the molecular basis of these characteristics and improving the efficiency of such breeding programmes require the development of genomic information and tools. However, despite its economic relevance, genomic information on olive or closely related species is still scarce. We have applied Sanger and 454 pyrosequencing technologies to generate close to 2 million reads from 12 cDNA libraries obtained from the Picual, Arbequina, and Lechin de Sevilla cultivars and seedlings from a segregating progeny of a Picual × Arbequina cross. The libraries include fruit mesocarp and seeds at three relevant developmental stages, young stems and leaves, active juvenile and adult buds as well as dormant buds, and juvenile and adult roots. The reads were assembled by library or tissue and then assembled together into 81 020 unigenes with an average size of 496 bases. Here, we report their assembly and their functional annotation.

A novel desiccation-tolerant, xeroprotectant-producing bacterium, designated strain 4J27T, was isolated from a Nerium oleander rhizosphere subjected to seasonal drought in Granada, Spain. Phylogenetic analysis based on 16S rRNA gene sequencing placed the isolate within the genus Arthrobacter , its closest relative being Arthrobacter phenanthrenivorans Shep3 DSM 18606T, with which it showed 99.23 % 16S rRNA gene sequence similarity. DNA–DNA hybridization measurements showed less than 25 % relatedness between strain 4J27T and Arthrobacter phenanthrenivorans DSM 18606T. The DNA base composition of strain 4J27T was 65.3 mol%. The main fatty acids were anteiso C15 : 0, anteiso C17 : 0, C16 : 0 and iso C16 : 0 and the major menaquinone was MK-9 (H2). The peptidoglycan type was A3α with an l-Lys–l-Ser–l-Thr–l-Ala interpeptide bridge. The bacterium tested positive for catalase activity and negative for oxidase activity. Phylogenetic, chemotaxonomic and phenotypic analyses indicated that the desiccation-tolerant strain 4J27T represents a novel species within the genus Arthrobacter , for which the name Arthrobacter siccitolerans is proposed. The type strain is 4J27T ( = CECT 8257T = LMG 27359T).

Viruses have been for long polemic biological particles which stand in the twilight of being living entities or not. As their genome is reduced, they rely on the metabolic machinery of their host in order to replicate and be able to continue with their infection process. The understanding of their metabolic requirements is thus of paramount importance in order to develop tailored drugs to control their population, without affecting the normal functioning of their host. New advancements in high throughput technologies, especially metabolomics are allowing researchers to uncover the metabolic mechanisms of viral replication. In this short review, we present the latest discoveries that have been made in the field and an overview of the intrinsic relationship between metabolism and innate immunity as an important part of the immune system.

The effect of salinity on the hydrolytic enzymatic activities (acid phosphatase, alkaline phosphatase, glucosidase, protease and esterase) released by the microorganisms in a submerged fixed bed bioreactor for real urban wastewater treatment was investigated. The influence of salt (NaCl) on the enzymatic activities was evaluated in four different experiments with concentrations of NaCl of 0, 3.7, 24.1 and 44.1g/L, remaining constant all other operating parameters of the bioreactor. The results show that enzymatic activities were reduced when the salinity was increased in the influent and consequently the biotransformation of organic matter in the submerged fixed bed bioreactor significantly decreased. A redundancy analysis was performed to evaluate the relationships between enzymatic activities and physic-chemical parameters analyzed in the influent. According to the results obtained with the Monte Carlo permutation test, salinity and sampling day significantly contributed to explain the variation of enzymatic activities, showing a negative correlation.

The purpose of this investigation was to determine the prevalence of plasmid-mediated AmpC (pAmpC) and carbapenemases in Enterobacteriaceae collected from 35 hospitals in Spain and to establish their epidemiological relationships. We conducted a prospective multi-centre study on pAmpC- or carbapenemase-producing Enterobacteriaceae isolates from clinical samples collected from February to July 2009. The strains suspected to carry pAmpC were resistant or showed intermediate susceptibility to co-amoxiclav and second- or third-generation cephalosporins. Strains suspected to carry a carbapenemase were selected because they showed a minimum inhibitory concentration (MIC) to imipenem >1 mg/L. Polymerase chain reaction (PCR) and a sequencing strategy were used to characterise the enzymes. The clonal relationships between isolates was analysed by pulsed field gel electrophoresis (PFGE). Among 100,132 Enterobacteriaceae isolates collected, 1,654 were compatible with the production of pAmpC or carbapenemases. We found a prevalence of 0.64 % of pAmpC (n = 635) and 0.04 % of carbapenemases (n = 43). The most prevalent pAmpC enzymes were CMY-type (78.3 %), DHA-type (19.5 %), ACC-type (1.6 %) and FOX-type (0.6 %). The CMY-type was the most frequent in Escherichia coli and Proteus mirabilis species, whereas the DHA-type was mainly found in Klebsiella spp. The enzymes involved in carbapenem resistance were VIM-1, IMP-22 and the new IMP-28. Nine new bla genes were described: bla CMY-54, bla CMY-55, bla CMY-56, bla CMY-57, bla CMY-96, bla DHA-6, bla DHA-7, bla FOX-8 and bla IMP-28. The prevalence of pAmpC or carbapenemases found is not negligible. The CMY-types were the predominant pAmpC, whereas the VIM or IMP enzymes were the predominant carbapenemases. Furthermore, we observed a great genetic diversity among pAmpC-producing strains and a close clonal relationship between carbapenemase-producing strains.