184 results on '"Cai, Yun"'
Search Results
2. Radical denitrogenative transformations of polynitrogen heterocycles: Building C–N bonds and beyond
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Zu-Li Wang, Cai-Yun Chen, Wen-Chao Yang, and Jun-Feng Li
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Annulation ,Chemical bond ,Chemistry ,Radical ,Synthon ,Photocatalysis ,General Medicine ,Bond formation ,Combinatorial chemistry ,Borylation ,Catalysis - Abstract
Polynitrogen heterocycles are readily available and have recently arisen as versatile synthons for the formation of various C–C and C–X bonds, and medicinally active nitrogen-containing heterocycles. Several cascade reactions, including annulation, radical cascade, and borylation reactions, have been reported in which polynitrogen heterocycles are applied as arylation reagents. The success of these exceptional reactions illustrates the great synthetic potential of polynitrogen heterocycles, which provides a direct and useful approach to arylation reactions and the synthesis of nitrogen-containing heterocycles. The use of photocatalysts to effectively transfer energy from visible light to non-absorbing compounds has gained increasing attention as this method allows for the mild and efficient generation of radicals in a controlled manner. This approach has thus led to new methods that involve unique bond formation reactions. In addition, the use of free radical intermediates stabilized by transition metal catalysts is a powerful way to construct new chemical bonds. The aim of this review is to highlight the rapidly expanding area of radical-initiated denitrogenative cascade reactions of polynitrogen heterocycles and elaborate on their mechanisms from a new perspective by using photocatalysis and metal-based catalysis.
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- 2021
3. Evaluation of physicochemical properties, bacterial community, and product fertility during rice straw composting supplemented with different nitrogen-rich wastes
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Shi-Peng Wang, Xiao-Xing Li, Zhao-Yong Sun, Wen-Liang Shuai, Zi-Yuan Xia, Cai-Yun Xie, Min Gou, and Yue-Qin Tang
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Environmental Engineering ,Renewable Energy, Sustainability and the Environment ,Bioengineering ,General Medicine ,Waste Management and Disposal - Abstract
This study evaluated the compostability of rice straw as the main feedstock (75% in dry weight), supplemented with three different nitrogen-rich wastes, namely food waste (FW), dairy manure (DM), and sewage sludge (SS). Organic matter (OM) degradation, maturity and fertility of the end-product, and bacterial community structure during the composting processes were compared. All composting processes generated mature end-product within 51 days. Notably, FW addition was more effective to accelerate rice straw OM degradation and significantly improved end-product fertility with a high yield of Chinese cabbage. The succession of the bacterial community was accelerated with FW supplementation. Genera Geobacillus, Chryseolinea, and Blastocatella were significantly enriched during the composting of rice straw with FW supplementation. Finally, temperature, total nitrogen, moisture, pH, and total carbon were the key factors affecting microorganisms. This study provides a promising alternative method to enhance the disposal of larger amounts of rice straw in a shorter time.
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- 2022
4. Identification and Validation of Four Novel Promoters for Gene Engineering with Broad Suitability across Species
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Ying-Cai Wu, Li-Cheng Liu, Cai-Yun Wang, and Yi-Xuan Zhang
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Green Fluorescent Proteins ,Gene Expression ,Cytochrome c Group ,Applied Microbiology and Biotechnology ,Sorbose dehydrogenase ,Metabolic engineering ,Bacterial Proteins ,Genes, Reporter ,Bacillus licheniformis ,Rhodobacteraceae ,Promoter Regions, Genetic ,Gene ,Genetics ,Bacteria ,Base Sequence ,biology ,Reproducibility of Results ,LacUV5 ,Promoter ,General Medicine ,biology.organism_classification ,Pseudomonas putida ,Metabolic Engineering ,Carbohydrate Dehydrogenases ,Biotechnology - Abstract
The transcriptional capacities of target genes are strongly influenced by promoters, whereas few studies have focused on the development of robust, high-performance and cross-species promoters for wide application in different bacteria. In this work, four novel promoters (Pk.rtufB, Pk.r1, Pk.r2, and Pk.r3) were predicted from Ketogulonicigenium robustum and their inconsistency in the -10 and -35 region nucleotide sequences indicated they were different promoters. Their activities were evaluated by using green fluorescent protein (gfp) as a reporter in different species of bacteria, including K. vulgare SPU B805, Pseudomonas putida KT2440, Paracoccus denitrificans PD1222, Bacillus licheniformis and Raoultella ornithinolytica, due to their importance in metabolic engineering. Our results showed that the four promoters had different activities, with Pk.r1 showing the strongest activity in almost all of the experimental bacteria. By comparison with the commonly used promoters of E. coli (tufB, lac, lacUV5), K. vulgare (Psdh, Psndh) and P. putida KT2440 (JE111411), the four promoters showed significant differences due to only 12.62% nucleotide similarities, and relatively higher ability in regulating target gene expression. Further validation experiments confirmed their ability in initiating the target minCD cassette because of the shape changes under the promoter regulation. The overexpression of sorbose dehydrogenase and cytochrome c551 by Pk.r1 and Pk.r2 resulted in a 22.75% enhancement of 2-KGA yield, indicating their potential for practical application in metabolic engineering. This study demonstrates an example of applying bioinformatics to find new biological components for gene operation and provides four novel promoters with broad suitability, which enriches the usable range of promoters to realize accurate regulation in different genetic backgrounds.
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- 2021
5. Thermophilic semi-continuous composting of kitchen waste: Performance evaluation and microbial community characteristics
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Shi-Peng Wang, Yang Gao, Zhao-Yong Sun, Xiang-Yu Peng, Cai-Yun Xie, and Yue-Qin Tang
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Actinobacteria ,Manure ,Soil ,Environmental Engineering ,Bacteria ,Renewable Energy, Sustainability and the Environment ,Composting ,Microbiota ,Bioengineering ,General Medicine ,Waste Management and Disposal - Abstract
This study evaluated the feasibility, system stability, and microbial community succession of thermophilic semi-continuous composting of kitchen waste (KW). The results revealed that treatment performance was stable at a 10 % feeding ratio, with an organic matter (OM) degradation efficiency of 81.5 % and seed germination index (GI) of 50.0 %. Moreover, the OM degradation efficiency and GI were improved to 83.4 % and 70.0 %, respectively, by maintaining an optimal compost moisture content (50-60 %). However, feeding ratios of ≥ 20 % caused deterioration of the composter system owing to OM overloading. Microbial community analysis revealed that Firmicutes, Actinobacteria, Chloroflexi, Proteobacteria, and Gemmatimonadetes were dominant. Additionally, moisture regulation significantly increased the Proteobacteria abundance by 57.1 % and reduced the Actinobacteria abundance by 57.8 %. Moreover, network analysis indicated that the bacterial community stability and positive interactions between genera were enhanced by moisture regulation. This information provides a useful reference for practical KW composting treatment in the semi-continuous mode.
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- 2022
6. Corrigendum to 'Low-dose phthalates promote breast cancer stem cell properties via the oncogene ΔNp63α and the Sonic hedgehog pathway' [Ecotoxicol. Environ. Saf. 252 (2023) 114605]
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Wan-shuang Cao, Meng-jiu Zhao, Yue Chen, Jian-yun Zhu, Chun-feng Xie, Xiao-ting Li, Shan-shan Geng, Cai-yun Zhong, Jin-yan Fu, and Jie-shu Wu
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Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,General Medicine ,Pollution - Published
- 2023
7. Valorizing kitchen waste to produce value-added fertilizer by thermophilic semi-continuous composting followed by static stacking: Performance and bacterial community succession analysis
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Xiang-Yu Peng, Shi-Peng Wang, Xiu-Lin Chu, Zhao-Yong Sun, Zi-Yuan Xia, Cai-Yun Xie, Min Gou, and Yue-Qin Tang
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Environmental Engineering ,Renewable Energy, Sustainability and the Environment ,Bioengineering ,General Medicine ,Waste Management and Disposal - Published
- 2023
8. Comprehensive chemical study on different organs of cultivated and wild Sarcandra glabra using ultra-high performance liquid chromatography time-of-flight mass spectrometry (UHPLC-TOF-MS)
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Jiang Zhi-Hong, Che Kai-Si, Chen Da-Xin, Zhang Wei, LU Jing-Guang, Zhong Ming, Wang Cai-Yun, and Wang Jing-Rong
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Plant Stems ,010405 organic chemistry ,Phytochemicals ,General Medicine ,Biology ,Plant Roots ,01 natural sciences ,Mass Spectrometry ,0104 chemical sciences ,Plant Leaves ,Magnoliopsida ,03 medical and health sciences ,Horticulture ,0302 clinical medicine ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,Chemical constituents ,Drug Discovery ,Ultra high performance ,Time-of-flight mass spectrometry ,Chromatography, High Pressure Liquid ,Sarcandra glabra - Abstract
To illuminate the similarities and differences between wild and cultivated Sarcandra glabra (S. glabra), we performed a comprehensively study on 26 batches of cultivated S. glabra and 2 batches of wild S. glabra. Chemical constituents and distribution characteristics of roots, stems and leaves in both wild and cultivated S. glabra were investigated through UHPLC-TOF-MS method. The result revealed that there were significant differences between roots, stems and leaves in S. glabra. And the chemical contents in the root part were less or even absence than those in leaf and stem, which suggested the root organ could be excluded as medicine. Meanwhile, the chemical contents of stems and leaves in cultivated S. glabra was sightly higher than that of wild samples. Therefore, cultivated S. glabra may have a high potential for substitution of wild S. glabra without affecting its pharmaceutical properties. In summary, our study could provide important information to the molecular basis for quality control of S. glabra.
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- 2021
9. Association Between White Matter Hyperintensities and Chronic Kidney Disease: A Systematic Review and Meta-Analysis
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Cun-Sheng Wei, Cai-Yun Yan, Xiao-Rong Yu, Lin Wang, Rui Zhang, Jun-Ying Jiang, Qi Dai, Jun-Rong Li, and Xue Mei Chen
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General Medicine - Abstract
ObjectivesPrevious studies of the associations between white matter hyperintensities (WMH) and chronic kidney disease (CKD) were still conflicting; therefore, our study aimed to conduct a systematic review of all of the available research on this topic and a meta-analysis of the association between WMH and CKD among observational studies.Setting and DesignSystematic review and meta-analysis.Outcome MeasuresSeverity of WMH.Methods and ParticipantsAll relevant studies in public databases were examined until 15 November 2020. Two independent reviewers assessed all the included studies using the Cross-Sectional/Prevalence Study Quality (CSSQ) scale, and then literature review and meta-analyses were undertaken.ResultsWe pooled the odds ratio (OR) for the presence of WMH, periventricular hyperintensities (PVH), and deep subcortical white matter hyperintensities (DWMH) of patients with CKD vs. non-CKD patients by subgroup analysis, and the results obtained were WMH OR 2.07, 95% CI [1.58, 2.70], PVH OR 2.41, 95% CI [1.90, 3.05], and DWMH OR 2.11, 95% CI [1.60, 2.80], respectively. The main outcome showed that patients with CKD were more likely to have WMH in the brain compared to the normal controls. Another meta-analysis showed a statistically significant decline in renal function in patients with moderate to severe WMH compared with those with no to mild WMH.ConclusionsThe findings indicated that patients with CKD were more likely to experience WMH than demographically matched controls. On the other hand, patients with moderate to severe WMH in the brain had poor renal function more frequently than those with no to mild WMH.
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- 2022
10. Type A aortic dissection developed after type B dissection with the presentation of shoulder pain: A case report
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Xiao-Kai Wang, Xin-Bo Yin, Su Xu, and Cai-Yun He
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Aortic dissection ,medicine.medical_specialty ,business.industry ,New type A aortic dissection ,Shoulder pain ,Misdiagnosis ,Emergency setting ,General Medicine ,medicine.disease ,Type b dissection ,Surgery ,Atypical symptoms ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Case report ,cardiovascular system ,medicine ,030211 gastroenterology & hepatology ,Presentation (obstetrics) ,Chronic type B aortic dissection ,business - Abstract
BACKGROUND Aortic dissection (AD) is a life-threatening condition with a high mortality rate without immediate medical attention. Early diagnosis and appropriate treatment are critical in treating patients with AD. In the emergency department, patients with AD commonly present with classic symptoms of unanticipated severe chest or back pain. However, it is worth noting that atypical symptoms of AD are easily misdiagnosed. CASE SUMMARY A 51-year-old woman was first diagnosed with scapulohumeral periarthritis due to left shoulder pain. After careful examination of her previous medical history and contrast-enhanced computed tomography angiography, the patient was diagnosed with a new type A AD after chronic type B dissection in the ascending aorta. The patient was successfully treated with surgical replacement of the dissected aortic arch and remains in good health. CONCLUSION New retrograde type A AD after chronic type B dissection is relatively rare. It is worth noting that a physician who has a patient with suspected AD should be vigilant. Both patient medical history and imaging tests are crucial for a more precise diagnosis.
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- 2021
11. Low-dose phthalates promote breast cancer stem cell properties via the oncogene ΔNp63α and the Sonic hedgehog pathway
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Wan-shuang Cao, Meng-jiu Zhao, Yue Chen, Jian-yun Zhu, Chun-feng Xie, Xiao-ting Li, Shan-shan Geng, Cai-yun Zhong, Jin-yan Fu, and Jie-shu Wu
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Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,General Medicine ,Pollution - Published
- 2023
12. Comparative efficacy of respiratory personal protective equipment against viral respiratory infectious diseases in healthcare workers: a network meta-analysis
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Su Xu, Xiao-Kai Wang, Xin-Bo Yin, and Cai-Yun He
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Adult ,medicine.medical_specialty ,Health Personnel ,Network Meta-Analysis ,Disease cluster ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,Viral respiratory infectious diseases ,law ,Surgical mask ,Health care ,medicine ,Healthcare workers ,Humans ,030212 general & internal medicine ,Respiratory Protective Devices ,Personal protective equipment ,Personal Protective Equipment ,Respiratory Tract Infections ,Review Paper ,business.industry ,SARS-CoV-2 ,030503 health policy & services ,Incidence (epidemiology) ,Public Health, Environmental and Occupational Health ,Masks ,COVID-19 ,Bayes Theorem ,Odds ratio ,General Medicine ,N95 respirator ,Systematic review ,Meta-analysis ,Emergency medicine ,Communicable Disease Control ,0305 other medical science ,business - Abstract
Objective With the epidemic of coronavirus disease 2019 (COVID-19), the healthcare workers (HCWs) require proper respiratory personal protective equipment (rPPE) against viral respiratory infectious diseases (VRIDs). It is necessary to evaluate which type of mask and manner of wearing is the best suitable rPPE for preventing the VRID. Study design A Bayesian network meta-analysis was performed to comprehensively analyze the protective efficacy of various rPPE. Methods This network meta-analysis protocol was registered in an international prospective register of systematic reviews (CRD42020179489). Electronic databases were searched for cluster randomized control trials (RCTs) of comparing the effectiveness of rPPE and wearing manner in preventing HCWs from VRID. The primary outcome was the incidence of laboratory-confirmed viral respiratory infection reported as an odds ratio (OR) with the associated 95% credibility interval (CrI). The secondary outcome was the incidence of clinical respiratory illness (CRI) reported as an OR with the associated 95% CrI. Surface under the cumulative ranking curve analysis (SUCRA) provided a ranking of each rPPE according to the primary outcome and the secondary outcome as data supplement. Results Six studies encompassing 12,265 HCWs were included. In terms of the incidence of laboratory-confirmed viral respiratory infection, the continuous wearing of N95 respirators (network OR, 0.48; 95% CrI: 0.27 to 0.86; SUCRA score, 85.4) showed more effective than the control group. However, in terms of reducing the incidence of CRI, there was no rPPE showing superior protective effectiveness. Conclusions There are significant differences in preventive efficacy among current rPPE. Our result suggests that continuous wearing of N95 respirators on the whole shift can serve as the best preventive rPPE for HCWs from the VRID., Highlights • First network meta-analysis of respiratory personal protective equipment (rPPE). • Wearing manner is important for susceptible healthcare workers. • Continuous wearing of N95 respirators on the whole shift can serve as the best preventive rPPE for healthcare workers from the VRIDs.
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- 2020
13. Serum ferritin levels in children with attention deficit hyperactivity disorder and tic disorder
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Cai-Yun Tang and Fang Wen
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General Medicine - Abstract
Iron plays an important role in neurodevelopmental functions in the brain. Serum ferritin levels are different in children with attention deficit hyperactivity disorder and tic disorder than in healthy children.To explore the current status of iron deficiency in children with neurodevelopmental disorders and its sex and age effects.A total of 1565 children with attention deficit hyperactivity disorder (ADHD), 1694 children with tic disorder (TD), 93 children with ASD and 1997 healthy control children were included between January 1, 2020, and December 31, 2021 at Beijing Children's Hospital. We describe the differences in age levels and ferritin levels between different disease groups and their sex differences. The differences between the sexes in each disease were analyzed using the t test. The incidence rate of low serum ferritin was used to describe the differences between different diseases and different age groups. A chi-square test was used to analyze the difference in the incidence of low serum ferritin between the disease group and the control group. Analysis of variance was used for comparisons between subgroups, and regression analysis was used for confounding factor control.A total of 1565 ADHD patients aged 5-12 years were included in this study, and the average serum ferritin levels of male and female children were 36.82 ± 20.64 μg/L and 35.64 ± 18.56 μg/L, respectively. A total of 1694 TD patients aged 5-12 years were included in this study, and the average serum ferritin levels of male and female children were 35.72 ± 20.15 μg/L and 34.54 ± 22.12 μg/L, respectively. As age increased, the incidence of low serum ferritin in ADHD and TD first decreased and then increased, and 10 years old was the turning point of rising levels. The incidence of ADHD with low serum ferritin was 8.37%, the incidence of TD with low serum ferritin was 11.04%, and the incidence of the healthy control group with low serum ferritin was 8.61%, among which male children with TD accounted for 9.25% and female children with TD accounted for 11.62%. There was a significant difference among the three groups (In conclusion, low serum ferritin is not a risk factor for ADHD or TD. The incidence of low serum ferritin levels in children with ADHD and TD between 5 and 12 years old decreases first and then increases with age.
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- 2022
14. Aiphanol, a multi-targeting stilbenolignan, potently suppresses mouse lymphangiogenesis and lymphatic metastasis
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Shan-mei Chen, Chuan-ke Zhao, Li-cheng Yao, Li-xin Wang, Yu-nan Ma, Lin Meng, Shao-qing Cai, Cai-yun Liu, Li-ke Qu, Yan-xing Jia, and Cheng-chao Shou
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Pharmacology ,Pharmacology (medical) ,General Medicine - Abstract
The high incidence of lymphatic metastasis is closely related to poor prognosis and mortality in cancers. Potent inhibitors to prevent pathological lymphangiogenesis and lymphatic spread are urgently needed. The VEGF-C-VEGFR3 pathway plays a vital role in driving lymphangiogenesis and lymph node metastasis. In addition, COX2 in tumor cells and tumor-associated macrophages (TAMs) facilitates lymphangiogenesis. We recently reported that aiphanol, a natural stilbenolignan, attenuates tumor angiogenesis by repressing VEGFR2 and COX2. In this study, we evaluated the antilymphangiogenic and antimetastatic potency of aiphanol using in vitro, ex vivo and in vivo systems. We first demonstrated that aiphanol directly bound to VEGFR3 and blocked its kinase activity with an half-maximal inhibitory concentration (IC
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- 2022
15. The anti-inflammatory effect of ent-kaur-15-en-17-al-18-oic acid on lipopolysaccharide-stimulated RAW264.7 cells associated with NF-κB and P38/MAPK pathways
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Zhuo Han, Wei-Dong Xie, Cai-Yun Zhang, Xia Li, Fang-Yuan Liu, Jia-Hui Ma, Shi-Qi Lin, and Fu-Juan Jia
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Pharmacology ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Organic Chemistry ,Pharmaceutical Science ,General Medicine ,Glutathione ,Malondialdehyde ,medicine.disease_cause ,Molecular biology ,Analytical Chemistry ,Nitric oxide ,Nitric oxide synthase ,Superoxide dismutase ,chemistry.chemical_compound ,Complementary and alternative medicine ,chemistry ,Drug Discovery ,biology.protein ,medicine ,Molecular Medicine ,Tumor necrosis factor alpha ,Oxidative stress - Abstract
Ent-kaur-15-en-17-al-18-oic acid (LL-3) was demonstrated that it can inhibit LPS-induced nitric oxide (NO) production and macrophage migration, maintain homeostasis of oxidative stress, including increased mitochondrial membrane potential (MMP), decreased levels of reactive oxygen species (ROS) and malondialdehyde (MDA), and maintenance of superoxide dismutase (SOD) and glutathione (GSH) activities and inhibit oxidative stress-induced P38 and nuclear factor κB (NF-κB) pathways to decrease inducible nitric oxide synthase (iNOS), cyclooxygense-2 (COX-2), and tumour necrosis factor (TNF)-α mRNA expressions without marked cytotoxicity. These findings revealed that LL-3 could serve as a candidate lead compound for further studying anti-inflammatory therapies.[Formula: see text].
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- 2020
16. Selbstorganisation in Wasser mit N‐substituierten Aminen
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Guangcheng Wu, Cai-Yun Wang, Ye Lei, Yang Zhang, Albert C. Fahrenbach, Libo Shen, Tianyu Jiao, and Hao Li
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Chemistry ,General Medicine - Published
- 2020
17. Assessment of sugar and sugar accumulation-related gene expression profiles reveal new insight into the formation of low sugar accumulation trait in a sweet orange (Citrus sinensis) bud mutant
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Syed Bilal Hussain, Cai-Yun Shi, Ling-Xia Guo, Ying-Xing Bai, Yong-Zhong Liu, Muhammad Abbas Khan, and Wei Du
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0301 basic medicine ,Citrus ,Sucrose ,Orange (colour) ,Carbohydrate metabolism ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Gene Expression Regulation, Plant ,Genetics ,Sugar transporter ,Food science ,Sugar ,Molecular Biology ,Gene Expression Profiling ,food and beverages ,Fructose ,General Medicine ,Sweetness ,Glucose ,030104 developmental biology ,chemistry ,Fruit ,030220 oncology & carcinogenesis ,Carbohydrate Metabolism ,Sugars ,Transcriptome ,Citrus × sinensis ,Citrus sinensis - Abstract
The accumulation of soluble sugars in fleshy fruits largely determines their sweetness or taste. A spontaneous sweet orange mutant 'Hong Anliu' (HAL, Citrus sinensis) accumulates low soluble sugar content in fruit juice sacs than its wild type, 'Anliu' (AL) orange; however, the cause of reduced sugar content in 'HAL' fruit remains unclear. In this study, sugar content and expression profiles of genes involved in sugar metabolism and transport were compared between 'HAL' and 'AL' fruit juice sacs. In both cultivars, fructose and glucose displayed the increasing trends with significantly lower contents in 'HAL' than 'AL' after 160 DAF; moreover, sucrose had a declining trend in 'HAL' and increasing trend in 'AL' with fruit development. On the other hand, transcript levels of VINV, CWINV1, CWINV2, SUS4, SUS5, SPS1, SPS2, VPP-1, VPP-2, and some sugar transporter genes were significantly decreased in 'HAL' compared with 'AL' after 100 DAF or 160 DAF. Interestingly, the transcript levels of SPS2 and SUT2 exhibited a similar trend as it was found for sucrose content in both cultivars. These results suggested that the low sugar accumulation in 'HAL' fruit JS is accompanied by the reduced sink strength, sucrose-synthesis ability, and vacuolar storage ability compared with 'AL'; reduction of CWINVs, VINV, SPS2, SUT2, VPP-1, and VPP-2 transcript levels possibly plays a key role in the low storage of soluble sugars in the vacuoles of mutant juice sacs.
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- 2020
18. Cloning, expression, and characterization of a novel heparinase I from Bacteroides eggerthii
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Cai-Yun Liu, Ye-Wang Zhang, Li-Bin Guo, and Wen-Bin Su
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Cloning ,chemistry.chemical_classification ,Chemistry ,medicine.drug_class ,Low molecular weight heparin ,General Medicine ,Heparin ,Oligosaccharide ,Biochemistry ,Bacteroides eggerthii ,medicine ,Heparinase I ,Unsaturated disaccharide ,Biotechnology ,medicine.drug - Abstract
Heparinase I (Hep I) specifically degrades heparin to oligosaccharide or unsaturated disaccharide and has been widely used in preparation of low molecular weight heparin (LMWH). In this wor...
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- 2020
19. Paternal uniparental disomy of chromosome 16 resulting in homozygosity of a GPT2 mutation causes intellectual and developmental disability
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Jing Liu, Baiyun Chen, Yuchun Liu, Jinghui Kong, Bo Zhang, Liang Han, Daoqi Mei, Cai Yun Ma, Qing Shang, Zhenhua Xie, Mengjun Xiao, Shiyue Mei, Yaodong Zhang, Chao Gao, and Dongxiao Li
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Developmental Disabilities ,Intellectual Disability ,Homozygote ,Genetics ,Humans ,General Medicine ,Uniparental Disomy ,Genetics (clinical) ,Chromosomes, Human, Pair 16 ,Transaminases - Abstract
Recessive mutations in glutamate pyruvate transaminase 2 (GPT2) have recently been found to be associated with intellectual and developmental disability (IDD). In this study, we discovered a homozygous missense variant, NM_133443: [c.1172C T, p. Pro391Leu], of GPT2 on chromosome 16 in a proband diagnosed with IDD through trio whole-exome sequencing (WES). The pathogenicity of the variant was further verified by bioinformatics analysis and functional studies in vitro. This autosomal recessive disease was caused by paternal uniparental disomy (UPD) which was further proven by single nucleotide polymorphism array (SNP array). In past literature, recessive diseases in chromosome 16 were usually due to maternal UPD where Mendel's law of inheritance was not applicable. However, in our case we found that paternal UPD can cause recessive diseases related to the GPT2 gene on chromosome 16. Our study provides an important line of evidence for the diagnosis of GPT2-related intellectual developmental disorders.
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- 2021
20. Research on Exhibition Service Quality Improvement Based on Exhibitors’ Perspective—Take the 42nd China Shandong Medical Expo as an Example
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Xiang-Tai Guan and Cai-Yun Tian
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General Medicine - Abstract
Exhibitors are the main body of the exhibition, and improving the exhibition’s service quality to win the exhibition’s satisfaction is an essential condition for the exhibition to obtain and maintain a competitive advantage. This paper introduces information into the SERVQUAL model, constructs an evaluation scale of 21 items, including six dimensions of tangibles, reliability, responsiveness, assurance, empathy, and information, and takes the 42nd Shandong Medical Expo as an example to evaluate and analyze the service quality of the exhibition. It is found that the expectation perception gap between reliability and responsiveness is the largest, the expectation perception gap between assurance and empathy is more significant, and the expectation perception gap between tangibles and information is the smallest. Finally, some suggestions and countermeasures are put forward from the aspects of improving the exhibition design, highlighting the characteristics of the exhibition, broadening the content of exhibition activities, enhancing the value of exhibitors, attaching importance to the perception of exhibitors, improving the level of service, using digital technology and strengthening the effect of exhibitors.
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- 2022
21. Whole-Exome Sequencing Among Chinese Patients With Hereditary Diffuse Gastric Cancer
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Ze-Xian, Liu, Xiao-Long, Zhang, Qi, Zhao, Yungchang, Chen, Hui, Sheng, Cai-Yun, He, Yu-Ting, Sun, Ming-Yu, Lai, Min-Qing, Wu, Zhi-Xiang, Zuo, Wei, Wang, Zhi-Wei, Zhou, Feng-Hua, Wang, Yu-Hong, Li, Rui-Hua, Xu, and Miao-Zhen, Qiu
- Subjects
Adult ,Male ,Adenosine Triphosphatases ,Ubiquitin-Protein Ligases ,East Asian People ,RNA-Binding Proteins ,General Medicine ,Adenocarcinoma ,Pedigree ,Cohort Studies ,MicroRNAs ,Stomach Neoplasms ,Exome Sequencing ,Humans ,Female ,Genetic Predisposition to Disease ,Retrospective Studies ,Transcription Factors - Abstract
ImportanceThe E-cadherin gene, CDH1, and the α-E-catenin gene, CTNNA1, were previously identified as hereditary diffuse gastric cancer (HDGC) susceptibility genes, explaining 25% to 50% of HDGC cases. The genetic basis underlying disease susceptibility in the remaining 50% to 75% of patients with HDGC is still unknown.ObjectiveTo assess the incidence rate of CDH1 germline alterations in HDGC, identify new susceptibility genes that can be used for screening of HDGC, and provide a genetic landscape for HDGC.Design, Setting, and ParticipantsThis cohort study conducted retrospective whole-exome and targeted sequencing of 284 leukocyte samples and 186 paired tumor samples from Chinese patients with HDGC over a long follow-up period (median, 21.7 [range, 0.6-185.9] months). Among 10 431 patients diagnosed with gastric cancer between January 1, 2002, and August 31, 2018, 284 patients who met the criteria for HDGC were included. Data were analyzed from August 1 to 30, 2020.Main Outcomes and MeasuresIncidence rate of CDH1 germline alterations, identification of new HDGC susceptibility genes, and genetic landscape of HDGC.ResultsAmong 284 Chinese patients, 161 (56.7%) were female, and the median age was 35 (range, 20-75) years. The frequency of CDH1 germline alterations was 2.8%, whereas the frequency of CDH1 somatic alterations was 25.3%. The genes with the highest incidence (>10%) of private germline alterations (including insertions and deletions) in the HDGC cohort were MUC4, ABCA13, ZNF469, FCGBP, IGFN1, RNF213, and SSPO, whereas previously reported germline alterations of CTNNA1, BRCA2, STK11, PRSS1, ATM, MSR1, PALB2, BRCA1, and RAD51C were observed at low frequencies (median, 4 [range, 1-12] cases). Furthermore, enrichment of the somatic variant signature of exposure to aflatoxin suggested potential interaction between genetics and environment in HDGC. Double-hit events in genes such as CACNA1D were observed, which suggested that these events might serve as important mechanisms for HDGC tumorigenesis. In addition, germline variants of FSIP2, HSPG2, and NCKAP5 and somatic alterations of FGFR3, ASPSCR1, CIC, DGCR8, and LZTR1 were associated with poor overall survival among patients with HDGC.Conclusions and RelevanceThis study provided a genetic landscape for HDGC. The study’s findings challenged the previously reported high germline alteration rate of CDH1 in HDGC and identified new potential susceptibility genes. Analyses of variant signatures and double-hit events revealed potentially important mechanisms for HDGC tumorigenesis. Findings from the present study may provide helpful information for further investigations of HDGC.
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- 2022
22. Inherited rare and common variants in PTCH1 and PTCH2 contributing to the predisposition to reproductive cancers
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Xin-Hua Yang, Bo-Heng Xu, Da-Lei Zhou, Ya-Kang Long, Qing Liu, Chan Huang, Zu-Lu Ye, and Cai-Yun He
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Adult ,Male ,Ovarian Neoplasms ,Genital Neoplasms, Female ,High-Throughput Nucleotide Sequencing ,Prostatic Neoplasms ,General Medicine ,Middle Aged ,Patched-2 Receptor ,Patched-1 Receptor ,Mutation Rate ,Genetics ,Genital Neoplasms, Male ,Humans ,Female ,Genetic Predisposition to Disease ,Germ-Line Mutation - Abstract
PTCH1 and PTCH2 are associated with nevoid basal cell carcinoma syndrome and basal cell carcinoma. We determined the prevalence of their common and rare variants in 877 patients with various reproductive cancers and 296 healthy subjects. Using targeted next-generation sequencing, we found significantly statistical associations of the minor alleles at seven common variants of PTCH1 and PTCH2 with a decreased risk of reproductive cancers (P = 9.69 × 10
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- 2021
23. Comparative Transcriptome Analysis Reveals Regulatory Networks during the Maize Ear Shank Elongation Process
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Hu Pei, Jun Huang, Gao-Ke Li, Ruichun Yang, Chang-Jian Liao, Cai-Yun Xiong, and Qing-You Gong
- Subjects
0106 biological sciences ,0301 basic medicine ,QH301-705.5 ,Quantitative Trait Loci ,Quantitative trait locus ,Biology ,maize ,01 natural sciences ,Zea mays ,Catalysis ,Article ,regulatory network ,Inorganic Chemistry ,Transcriptome ,03 medical and health sciences ,chemistry.chemical_compound ,Auxin ,Gene Expression Regulation, Plant ,otorhinolaryngologic diseases ,Xyloglucan:xyloglucosyl transferase ,Gene Regulatory Networks ,Biology (General) ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,Transcription factor ,Gene ,Spectroscopy ,Plant Proteins ,ear shank length ,chemistry.chemical_classification ,Gene Expression Profiling ,Organic Chemistry ,Gene Expression Regulation, Developmental ,General Medicine ,Computer Science Applications ,Cell biology ,Xyloglucan ,body regions ,Chemistry ,030104 developmental biology ,Gene Ontology ,Phenotype ,chemistry ,Gibberellin ,sense organs ,transcriptome ,010606 plant biology & botany ,Transcription Factors - Abstract
In maize, the ear shank is a short branch that connects the ear to the stalk. The length of the ear shank mainly affects the transportation of photosynthetic products to the ear, and also influences the dehydration of the grain by adjusting the tightness of the husks. However, the molecular mechanisms of maize shank elongation have rarely been described. It has been reported that the maize ear shank length is a quantitative trait, but its genetic basis is still unclear. In this study, RNA-seq was performed to explore the transcriptional dynamics and determine the key genes involved in maize shank elongation at four different developmental stages. A total of 8145 differentially expressed genes (DEGs) were identified, including 729 transcription factors (TFs). Some important genes which participate in shank elongation were detected via function annotation and temporal expression pattern analyses, including genes related to signal transduction hormones (auxin, brassinosteroids, gibberellin, etc.), xyloglucan and xyloglucan xyloglucosyl transferase, and transcription factor families. The results provide insights into the genetic architecture of maize ear shanks and developing new varieties with ideal ear shank lengths, enabling adjustments for mechanized harvesting in the future.
- Published
- 2021
24. Analysis of the Sagittal Motion Posture of the Acromioclavicular Joint Using Image Registration and Axial Angle Representation
- Author
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Jun-lin Zhou, Feng Liu, Yi Zhang, Peng Su, and Cai Yun
- Subjects
Orthodontics ,musculoskeletal diseases ,business.industry ,Image registration ,International Journal of General Medicine ,General Medicine ,030204 cardiovascular system & hematology ,Sagittal plane ,03 medical and health sciences ,image registration ,0302 clinical medicine ,medicine.anatomical_structure ,axial angle representation ,030220 oncology & carcinogenesis ,Horizontal position representation ,medicine ,acromioclavicular joints ,Upper limb ,Acromioclavicular joint ,Tomography ,Range of motion ,business ,Rotation (mathematics) ,Original Research - Abstract
Peng Su,1,2 Jun-lin Zhou,2 Cai Yun,1 Feng Liu,1 Yi Zhang1 1Department of Orthopaedics, Teaching Hospital of Capital Medical University, Beijing Shijingshan Hospital, Beijing, People’s Republic of China; 2Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Jun-lin ZhouDepartment of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, People’s Republic of ChinaTel +86-13240718193Email zhoujunlin145@163.comObjective: This study aimed to directly and accurately measure the range of motion of the acromioclavicular joint through 3D reconstruction and image registration.Methods: Thirteen healthy volunteers participated in the study. Computerized tomography (CT) was used to measure the acromioclavicular joint in four different motion poses. The images were integrated using reconstruction and registration technology, and the rotation angle range of the acromioclavicular joint was measured using 3D reconstruction. The measurements were expressed by axial angle representation. The dominant and the non-dominant sides were compared, and the difference in the axial angle of the acromioclavicular joint was compared in different postures.Results: The difference between the dominant and non-dominant sides in acromioclavicular motion was not significant. In the sagittal motion of the upper limb, the rotation angle of the acromioclavicular joint was greatest in a resting horizontal position, with an average of 26.1°. In this position, 34.6% of flexion and extension movements of the upper limb were completed by the acromioclavicular joint, which was the highest level of engagement. The rotation angle was lowest in the resting posteroinferior position, with an average of 8.2°. In this position, 24.9% of the flexion and extension movements of the upper limb were completed by the acromioclavicular joint.Conclusion: Combining 3D reconstruction and image registration is a direct and accurate method of measuring the motion of the acromioclavicular joint. The rotational motions of the acromioclavicular joint are bilaterally symmetrical and can be used as a reference for comparative study. The maximum range of motion of the acromioclavicular joint during rotation was found in the resting horizontal position. The clinical examination and post-treatment evaluation of the acromioclavicular joint’s rotation function can therefore be targeted in this range.Keywords: image registration, axial angle representation, acromioclavicular joints
- Published
- 2021
25. Role of introduced surface cysteine of NADH oxidase from Lactobacillus rhamnosus
- Author
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Cai-Yun Liu, Qing-Lan Tao, Fei-Long Li, Ye-Wang Zhang, and Jian Gao
- Subjects
Surface Properties ,Dimer ,Mutant ,02 engineering and technology ,medicine.disease_cause ,Biochemistry ,Protein Structure, Secondary ,Catalysis ,03 medical and health sciences ,chemistry.chemical_compound ,Residue (chemistry) ,Lactobacillus rhamnosus ,Multienzyme Complexes ,Structural Biology ,medicine ,NADH, NADPH Oxidoreductases ,Cysteine ,Molecular Biology ,030304 developmental biology ,Alanine ,0303 health sciences ,biology ,Lacticaseibacillus rhamnosus ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Molecular Docking Simulation ,Kinetics ,chemistry ,Mutation ,Biocatalysis ,Mutagenesis, Site-Directed ,0210 nano-technology ,Oxidative stress - Abstract
Cysteine, a critical residue for catalytic process but also vulnerable to oxidative damage, was conventionally expressed as a buried catalytic site in most redox enzymes. In the present work, specific surface-exposed sites of a NADH oxidase from Lactobacillus rhamnosus (LrNox) were selected and mutated to cysteine to investigate its effects on catalytic function because LrNox has a buried catalytic cysteine but no surface-exposed one. The results showed that exception of the sites on dimer interface, the activities of LrNox mutants were improved to vary degrees when the polar uncharged and alanine residues were mutated to cysteine. But the cysteine mutations of polar charged and nonpolar residues except alanine showed obvious decline in catalytic activity. Substituting of Ala85 and Thr96 with other residues suggested that the cysteine mutation showed the highest activity. Structural analysis suggested that even a single cysteine mutation on the specific non-conserved surface area of LrNox could induce changes on the conformation of catalytic cysteine and lower the activation free energy to improve the catalytic activity.
- Published
- 2019
26. Comparative Transcriptome Analysis of Recombinant Industrial Saccharomyces cerevisiae Strains with Different Xylose Utilization Pathways
- Author
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Zi-Yuan Xia, Bo Wu, Yun-Cheng Li, Bai-Xue Yang, Min Gou, Yue-Qin Tang, Cai-Yun Xie, and Zhao-Yong Sun
- Subjects
Xylose isomerase ,Saccharomyces cerevisiae ,DNA, Recombinant ,Bioengineering ,Xylose ,Ethanol fermentation ,Applied Microbiology and Biotechnology ,Biochemistry ,Transcriptome ,chemistry.chemical_compound ,Mitotic cell cycle ,Xylose metabolism ,Industry ,Molecular Biology ,Gene ,biology ,Chemistry ,Gene Expression Profiling ,General Medicine ,biology.organism_classification ,Fermentation ,Transcription Factors ,Biotechnology - Abstract
A heterologous xylose utilization pathway, either xylose reductase-xylitol dehydrogenase (XR-XDH) or xylose isomerase (XI), is usually introduced into Saccharomyces cerevisiae to construct a xylose-fermenting strain for lignocellulosic ethanol production. To investigate the molecular basis underlying the effect of different xylose utilization pathways on the xylose metabolism and ethanol fermentation, transcriptomes of flocculating industrial strains with the same genetic background harboring different xylose utilization pathways were studied. A different source of xylA did not obviously affect the change of the strains transcriptome, but compared with the XR-XDH strain, several key genes in the central carbon pathway were downregulated in the XI strains, suggesting a lower carbon flow to ethanol. The carbon starvation caused by lower xylose metabolism in XI strains further influenced the stress response and cell metabolism of amino acid, nucleobase, and vitamin. Besides, the downregulated genes mostly included those involved in mitotic cell cycle and the cell division-related process. Moreover, the transcriptomes analysis indicated that the after integrate xylA in the δ region, the DNA and chromosome stability and cell wall integrity of the strains were affected to some extent. The aim of this was to provide some reference for constructing efficient xylose-fermenting strains.
- Published
- 2019
27. Withaminimas A—F, six withanolides with potential anti-inflammatory activity from Physalis minima
- Author
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Cai-Yun Gao, Shanshan Wei, Ling-Yi Kong, Jun Luo, and Rui-Jun Li
- Subjects
Lipopolysaccharides ,Physalis ,medicine.drug_class ,Stereochemistry ,Anti-Inflammatory Agents ,01 natural sciences ,Anti-inflammatory ,Nitric oxide ,Mice ,Structure-Activity Relationship ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Drug Discovery ,medicine ,Ic50 values ,Animals ,Withanolides ,Molecular Structure ,biology ,010405 organic chemistry ,Macrophages ,General Medicine ,biology.organism_classification ,0104 chemical sciences ,RAW 264.7 Cells ,Complementary and alternative medicine ,chemistry ,030220 oncology & carcinogenesis ,Drugs, Chinese Herbal - Abstract
Withaminimas A-F (1-6), six new withaphysalin-type withanolides were isolated from the aerial parts of Physalis minima L.. The structures of these compounds were elucidated through a variety of spectroscopic techniques including HR-MS, NMR, and ECD. Compound 1 belongs to rare 18-norwithanolides, and 2-3 were 13/14-secowithanolides. According to the traditional usage of P. minima, inhibitory effects on nitric oxide (NO) production in lipopolysaccaride-activated RAW264.7 macrophages were evaluated, and compounds 1-4 exhibited significant inhibitory effects with IC50 values among 3.91-18.46 μmol·L-1.
- Published
- 2019
28. Soluble guanylate cyclase (sGC) stimulator vericiguat alleviates myocardial ischemia-reperfusion injury by improving microcirculation
- Author
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Cai, Yun, Zhang, Beijian, Shalamu, Adilan, Gao, Tingwen, and Ge, Junbo
- Subjects
Original Article ,General Medicine - Abstract
BACKGROUND: This study aimed to verify the effect of soluble guanylate cyclase (sGC) stimulator vericiguat on myocardial ischemia-reperfusion injury and explore its mechanism. METHODS: A myocardial ischemia-reperfusion injury model of mice was established and intravenous administration was performed 2 minutes before reperfusion. Triphenyltetrazolium chloride (TTC) staining and echocardiography were used to verify the effect of vericiguat on myocardial ischemia-reperfusion injury in the infarct area, and immunofluorescence was used to observe myocardial pathological changes at different time points after reperfusion. Quantitative proteomics was conducted to analysis the main differentially expressed proteins after drug intervention. The distribution of endothelial cells and sGC after myocardial ischemia-reperfusion injury in mice was observed by immunofluorescence. RNA sequencing of endothelial cells was used to search for differentially expressed molecules. Thioflavin-S staining was used to observe the effect of vericiguat on improving the nonrecurrence phenomenon and reducing the infarct size after reperfusion. RESULTS: The effect of the sGC stimulator vericiguat on myocardial ischemia-reperfusion injury was verified, and myocardial microcirculation significantly increased after drug intervention. Quantitative proteomics found that the protein expression of myocardial tissue in the ischemia-reperfusion area was not significantly different in the drug intervention group, except for increased adenosine triphosphate (ATP) activity. Vericiguat, nitroglycerin, and nitrite did not directly affect apoptosis or cell viability. RNA sequencing of human umbilical vein endothelial cells screened the upregulated antioxidant response. CONCLUSIONS: SGC stimulator vericiguat ameliorated myocardial ischemia-reperfusion injury through indirect pathways of improving microcirculation.
- Published
- 2022
29. A new flavanol from the roots of Daphne genkwa
- Author
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Cai-Yun Liu, Ling Tong, Cong Guo, and Rui Wang
- Subjects
Pharmacology ,Daphne genkwa ,biology ,Traditional medicine ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Pharmaceutical Science ,General Medicine ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Complementary and alternative medicine ,Phytochemical ,Drug Discovery ,Molecular Medicine ,Thymelaeaceae ,Derivative (chemistry) - Abstract
The phytochemical investigation of the roots of Daphne genkwa yielded six secondary metabolites, including a new flavanol derivative, (2R, 3S)-5,7,4′-trihydroxy-8-methoxycarbonylflavanol (1...
- Published
- 2018
30. Genetically Encoding a Lipidated Amino Acid for Extension of Protein Half-Life in vivo
- Author
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Liu Yang, Cai-Yun Fu, Qianqian Zhao, Qi Chen, Feng Zheng, Qian Wang, Lei Wang, Haorong Li, and Xiumei Wang
- Subjects
0301 basic medicine ,Serum albumin ,Stimulation ,010402 general chemistry ,Protein lipidation ,01 natural sciences ,Catalysis ,Mice ,03 medical and health sciences ,Glucagon-Like Peptide 1 ,In vivo ,Escherichia coli ,medicine ,Animals ,Amino Acids ,Gene ,chemistry.chemical_classification ,Mice, Inbred ICR ,biology ,010405 organic chemistry ,Lysine ,General Medicine ,General Chemistry ,Human serum albumin ,Lipids ,0104 chemical sciences ,Amino acid ,030104 developmental biology ,chemistry ,Biochemistry ,Genetic Code ,biology.protein ,Function (biology) ,Half-Life ,medicine.drug - Abstract
Protein therapeutics are increasingly used to treat various diseases, yet they often suffer from short serum half-lives. An emerging strategy to extend lifetime in vivo is to attach fatty acids onto proteins to increase their binding to human serum albumin (HSA). Herein, the genetic encoding of ϵ-N-heptanoyl-l-lysine (HepoK) is reported, which introduces a fatty-acid-containing amino acid into proteins with exquisite site-specificity and homogeneity, overcoming issues associated with existing chemical conjugation methods. The expression in E .coli and purification of HepoK-incorporated glucagon-like peptide-1 (GLP1) is demonstrated. GLP1(HepoK) showed stronger binding to HSA than GLP1(WT), without impairing the stimulation of the GLP1 receptor in cells. Moreover, GLP1(HepoK) decreased blood glucose level to the same level as GLP1(WT) in mice, showing longer-lasting effects than GLP1(WT). HepoK incorporation will also be useful for investigating the function of protein lipidation.
- Published
- 2018
31. Abstracts From the 13th World Congress of the World Federation of Critical Care Nurses and 10th Congress of the Serbian Society of Intensive Care, Anesthaesiology and Resuscitation (UINARS), Belgrade, Serbia, 25–28 October 2018
- Author
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Jun Zhong, Lin Zhao, Na Zhang, Samela Zelic, Meng Yu, Susan Yeager, Li Yao, Yu Xu, Nana Xu, Yuchen Wu, Ged Williams, Chunyan Wang, Rong Wang, Limei Wang, Maja Vuckovic, Kathleen Vollman, Sandro Vidmanić, Gulay Altun Ugras, Ayşe Uçar, Işik MeryemTürkan, Yongming Tian, Rósa Thorsteinsdóttir, Jianhua Sun, Serpil Akbaş Sü, Emina Stojanovic, Mirela Stefanovic, Jelena Slijepčević, Marija Simic, Li Shi, Han Sheng, Kurşun Şerife, Sasa Seferovic, Alicia San José, Faydalı Saide, Maria Isabelita Rogado, Lana Rodić, Sasa Ristic, Damir Peličić, Gordana Panova, Marjetka Nemes, Perver Mustu, Marcelo Morales, Marion Mitchell, Mirjana Meštrović, Violeta Lopez, Sarah Livesay, Huan Liu, Yao Liao, Qing Li, Zorica Lazarevic, Rodić Lana, Sofija Kurtović, Sibel Küçük, Burcu Küççük, Ruth Kleinpell, Wenwen Jing, Radmilo Jankovic, Sanja Ivankovic, Martina Ivanišić, Sandra Goldsworthy, Paul Fulbrook, Adriano Friganovic, Mario Dugonjić, Dubravac Elmedin, Aiping Du, Gordana Dragosev, Dobrović Dragica, Dušanka Cvijanović, Isabel Coetzee, Dilek Cingil, Mathilde Elsa Christensen, Xiuwan Chen, Burcu Ceylan, Shuyin Cao, Eva Barkestad, Marina Ahmetbegovic, Laila Abeddin, Hui Yang, Liming Li, Shichao Zhu, Yongju Pei, Mustafa Semiz, Haihua Zhu, Maria Ingrid Bianca Klette, Britt Sætre Hansen, Ayşenur Gürel, Gülfidan Başer, Filiz Hisar, Jelena Milovanovic, Maletic Mirjana, Biljana Savic, Gutovic Miljana, Sofija Kurtovic, Mirjana Maletic, Daliborka Dimitric, Biljana Savić, Dunja Majstorović, Emanuela Marcucci, Laura Alberto, Nicola Witton, Gajnok Šarika, Akin Belgin, Alime Selçuk Tosun, Dilek Uludasdemir, Ayşe Özeflanili, Marijana Neuberg, Kristina Kišić, Dijana Hereković, Anita Lukić, Branko Lukic, Xiaoying Wu, Xi Jin, Zhi Guo, Jelena Vrgoč, Mario Bespaljko, Ivan Lončar, Sibel Kucuk, Jasna Boshkovska, Blagica Panova, Dragica Dobrovic, Danica Stamenkovic, Miljana Stankovic, Nikola Antonijevic, Büşra Çakir, Özlem Çiftçi, Hongyan Zhang, Limei Zhang, Xueying Liu, Qingyin Li, Feifei Zhuang, Lin Zhang, Marija Momcilovic, Jagica Jovanovic, Suzana Jankovic, Nesime Demirören, Qing Zhang, Hailing Guo, Hong Sun, Xiaoting Wang, Gülay Yıldırım, Selda Arslan, Meryem Turkan Isik, Hasan Serinol, Sabina Babic, Nikolina Vratan, Sanja Jelic, Huijie Yu, Jianmei Xia, Zhigang Zhang, Caiyun Zhang, Jinhui Tian, Hao Wang, Min Zhou, A. Du, Y. Tian, Zhi-Gang Zhang, Jin-Hui Tian, Cai-Yun Zhang, Nannan Ding, D. Huang, P. Yan, Q. Li, Qing Yin Li, ChunYing Huo, YunXIA Hao, Lin Liu, Jili Zheng, Yan Xue, and Jingyi Zhang
- Subjects
Resuscitation ,Nursing ,Political science ,Critical care nursing ,Intensive care ,language ,General Medicine ,Serbian ,language.human_language - Published
- 2018
32. Genome-wide identification and transcript analysis of vacuolar-ATPase genes in citrus reveal their possible involvement in citrate accumulation
- Author
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Dong-Yuan Ning, Cai-Yun Shi, Huan Yang, Yong-Zhong Liu, Syed Bilal Hussain, and Ling-Xia Guo
- Subjects
0106 biological sciences ,0301 basic medicine ,Citrus ,Vacuolar Proton-Translocating ATPases ,Transcription, Genetic ,Protein subunit ,Plant Science ,Vacuole ,Horticulture ,Biology ,01 natural sciences ,Biochemistry ,Genome ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Transcription (biology) ,Citrates ,Molecular Biology ,Gene ,food and beverages ,Ripening ,General Medicine ,030104 developmental biology ,chemistry ,Citric acid ,Citrus × sinensis ,010606 plant biology & botany - Abstract
The vacuolar H+-ATPase (V-ATPase) proton pump plays an important role in the acidification of vacuoles; however, genes encoding V-ATPase in the citrus genome and their roles in citric acid accumulation remain unclear in citrus fruit. In this study, we found at least one gene encoding subunit A, B, C, D, G, c'', d or e; two genes encoding the subunit E, F, H or a; and four genes encoding subunit c in the citrus genome. Spatial expression analysis showed that most genes were predominantly expressed in the mature leaves and/or flowers but were less expressed in root and juice cells. Two sweet orange (Citrus sinensis) cultivars, 'Anliu' (AL) and 'Hong Anliu' (HAL), which differ in terms of fruit acidity, were used in this study. The citric acid content was significantly higher in 'AL' fruits than in 'HAL' fruits over the entire experimental period (82 days-236 days after full blossom, DAFB). Transcript analysis showed that the transcript levels of most subunit genes, including V1-A, V1-B, V1-C, V1-E1, V1-G, V1-H2 and V0-a2, V0-c", V0-c4, and V0-d, were significantly lower in 'HAL' than in 'AL' fruits during fruit development and ripening. Moreover, ABA injection significantly increased the citric acid content, simultaneously accompanied by the obvious induction of V1-A, V1-C, V1-E1, V1-F1, V1-H2, V0-a1, V0-a2, V0-c1, V0-c2, V0-c4, and V0-d transcription levels. In conclusion, the results demonstrated that V1-A, V1-C, V1-E1, V1-H2, V0-a2, V0-c4, and V0-d may play more roles than other subunit genes in the vacuole acidification of citrus fruits. The lower activity of V-ATPase caused by the transcript reduction of some subunit genes may be one reason for the low citrate accumulation in 'HAL' juice sacs.
- Published
- 2018
33. Effects of a yeast-derived product on growth performance, antioxidant capacity, and immune function of broilers
- Author
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Qi Ming Li, Zai Bin Yang, Kang Cheng, Yi Chun Tong, Tian Wang, Cai Yun Yu, Ting Wang, and Chao Wang
- Subjects
Immunoglobulin A ,Male ,GPX1 ,Saccharomyces cerevisiae ,antioxidant capacity ,Feed conversion ratio ,SF1-1100 ,Immunoglobulin G ,Antioxidants ,Superoxide dismutase ,Animal science ,yeast-derived product ,METABOLISM AND NUTRITION ,Animals ,immune function ,chemistry.chemical_classification ,growth performance ,biology ,Glutathione peroxidase ,Broiler ,Immunity ,General Medicine ,Animal Feed ,Yeast ,Animal culture ,Diet ,broiler chicken ,chemistry ,Dietary Supplements ,biology.protein ,Animal Science and Zoology ,Chickens - Abstract
Yeast culture plus enzymatically hydrolyzed yeast cell wall (YC-EHY) contains crude protein, mannan-oligosaccharide, β-glucan and yeast culture. This study was carried out to explore the effects of dietary YC-EHY at different levels on growth performance, antioxidant capacity, and immune function of broiler chickens. A total of 320 one-day-age male broiler chicks were allocated into 4 groups and were fed with a basal diet supplemented with 0 mg/kg (the control group), 50 mg/kg, 100 mg/kg, 150 mg/kg YC-EHY for 42 d. Dietary YC-EHY improved average daily gain and feed efficiency during the starter, grower, and overall periods as well as average body weight of broiler chickens on 42 d (linear and quadratic, P < 0.05). Broiler chickens fed with YC-EHY quadratically increased jejunal sucrase activity on 21 d (quadratic, P < 0.05), and linearly and quadratically enhanced maltase activity on 21 and 42 d (linear and quadratic, P < 0.05). Supplementing YC-EHY linearly and quadratically enhanced jejunal superoxide dismutase (SOD) activity on 21 and 42 d and glutathione peroxidase (GPX) activity on 42 d whereas decreased malonaldehyde (MDA) concentration on 42 d (linear and quadratic, P < 0.05). Consistently, the jejunal genes expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and SOD1 on 21 and 42 d, heme oxygenase-1 (HO-1) and GPX1 on 42 d were enhanced by YC-EHY supplementation (linear and quadratic, P < 0.05). The concentrations of jejunal immunoglobulin G (IgG) on 21 and 42 d and secreted immunoglobulin A (SIgA) on 42 d were linearly and quadratically elevated by supplementing YC-EHY (linear and quadratic, P < 0.05). Dietary YC-EHY quadratically increased jejunal IgG and IgM genes expression on 21 d (quadratic, P < 0.05), and linearly and quadratically enhanced the genes expression of IgG and IgM on 42 d (linear and quadratic, P < 0.05). Overall, this study indicated that supplementing YC-EHY could exert beneficial effects on growth performance, intestinal antioxidant capacity and immune function in broiler chickens.
- Published
- 2021
34. S100P acts as a target of miR-495 in pancreatic cancer through bioinformatics analysis and experimental verification
- Author
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Peng-Fei Jiang, Cai-Yun Song, Yan Wu, Xiu-Ju Zhang, and Yan-Xi Zhang
- Subjects
Medicine (General) ,Epithelial-Mesenchymal Transition ,Bioinformatics analysis ,Microarray ,pancreatic cancer ,Apoptosis ,Adenocarcinoma ,03 medical and health sciences ,0302 clinical medicine ,R5-920 ,Downregulation and upregulation ,Pancreatic cancer ,Cell Line, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,3' Untranslated Regions ,Cell Proliferation ,Gene knockdown ,business.industry ,Calcium-Binding Proteins ,Computational Biology ,General Medicine ,medicine.disease ,invasion ,Neoplasm Proteins ,Blot ,Pancreatic Neoplasms ,MicroRNAs ,HEK293 Cells ,miRNA‐495 ,030220 oncology & carcinogenesis ,Cancer research ,Disease Progression ,030211 gastroenterology & hepatology ,Calcium ,business ,S100P ,Protein Binding - Abstract
S100 calcium binding protein P (S100P) and miR‐495 are aberrantly expressed and exert essential roles in cancers. However, the mechanisms of miR‐495‐S100P in pancreatic cancer are yet to be illustrated. Thus, we explored the regulatory functions of miR‐495‐S100P axis in pancreatic adenocarcinoma cells growth and invasion. In this study, we identified that S100P was upregulated in pancreatic adenocarcinoma by bioinformatics analysis of the GEO (Gene Expression Omnibus database) microarray dataset (GSE16515). Western blotting and luciferase reporter gene analysis exhibited that miR‐495 negatively determined the level of S100P via binging to its 3′‐untranslated regions (3′‐UTRs). A series of functional experiments indicated that upregulation of miR‐495 or S100P knockdown suppressed pancreatic adenocarcinoma cells proliferation, invasion, and promoted apoptosis. Furthermore, the expression of S100P was negatively associated with the level of miR‐495 in The Cancer Genome Atlas (TCGA) pancreatic adenocarcinoma case‐cohort. Besides, reintroduction of S100P debilitated the anti‐cancer action of miR‐495 in pancreatic adenocarcinoma cells. Our data indicated that miR‐495 performed suppressive roles in pancreatic adenocarcinoma through targeting S100P.
- Published
- 2021
35. The chemical mechanisms of the enzymes in the branched-chain amino acids biosynthetic pathway and their applications
- Author
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Wen-Yun Gao, Ya-Jian Zhang, Le-Tian Yan, Cai-Yun Luo, Zi-Xian Long, Heng Li, and Yan-Fei Liang
- Subjects
0301 basic medicine ,chemistry.chemical_classification ,Carbon chain ,030102 biochemistry & molecular biology ,Mechanism (biology) ,Lab scale ,General Medicine ,Biochemistry ,Amino acid ,Biosynthetic Pathways ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Enzyme ,chemistry ,Biosynthesis ,Chain (algebraic topology) ,Animals ,Humans ,Organic synthesis ,Amino Acids, Branched-Chain - Abstract
l-Valine, l-isoleucine, and l-leucine are three key proteinogenic amino acids, and they are also the essential amino acids required for mammalian growth, possessing important and to some extent, special physiological and biological functions. Because of the branched structures in their carbon chains, they are also named as branched-chain amino acids (BCAAs). This review will highlight the advance in studies of the enzymes involved in the biosynthetic pathway of BCAAs, concentrating on their chemical mechanisms and applications in screening herbicides and antibacterial agents. The uses of some of these enzymes in lab scale organic synthesis are also discussed.
- Published
- 2020
36. Impact of ultrasound in diagnosis of hydatidiform mole in early pregnancy: A protocol of systematic review
- Author
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Cai-Yun An and Li Li
- Subjects
Research design ,medicine.medical_specialty ,MEDLINE ,PsycINFO ,Cochrane Library ,Ultrasonography, Prenatal ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,medicine ,Humans ,Medical physics ,030212 general & internal medicine ,Protocol (science) ,business.industry ,Ultrasound ,General Medicine ,Hydatidiform Mole ,Clinical trial ,Study heterogeneity ,Research Design ,030220 oncology & carcinogenesis ,Uterine Neoplasms ,Female ,business ,Systematic Reviews as Topic - Abstract
Background This study aims to assess current evidence of ultrasound in diagnosis of hydatidiform mole (HM) in early pregnancy (EP). Methods This study will incorporate case-control study on investigating the impact of ultrasound in diagnosis of HM in EP. Potential articles will be retrieved in electronic databases of Cochrane Library, MEDLINE/PUBMED, EMBASE, PsycINFO, WANGFANG, and CNKI from inception to the present. Conference proceeding, website of clinical trial registry, and reference list of key articles will be examined for additional studies. Two independent researchers will scan and select studies, collect and manage data, and appraise methodological quality of all eligible studies. We will carry out summary effect size, statistical heterogeneity, synthesize, and analyze outcome data. Results This study will summarize present evidence to assess the accuracy of ultrasound in diagnosis of HM in EP. Conclusion This study may provide evidence for ultrasound in diagnosis of HM in EP, which may benefit both patients and clinicians. Study registration INPLASY202080080.
- Published
- 2020
37. Circadian rhythm and atherosclerosis (Review)
- Author
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Cai-Yun Luo, Xin-An Wang, Tian Zhou, Zai-Qiang Zhang, Xia-Xia Zheng, Bin Yu, and Jiawang Ding
- Subjects
0301 basic medicine ,circadian rhythm ,Cancer Research ,Energy metabolism ,Inflammation ,Review ,Bioinformatics ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Immunology and Microbiology (miscellaneous) ,Medicine ,vascular smooth muscle cells ,Circadian rhythm ,Endothelial dysfunction ,business.industry ,General Medicine ,medicine.disease ,Molecular medicine ,endothelial cells ,macrophages ,030104 developmental biology ,030220 oncology & carcinogenesis ,medicine.symptom ,business ,Vascular function - Abstract
Atherosclerosis is the leading cause of morbidity and mortality worldwide. The underlying pathogenesis involves multiple metabolic disorders, endothelial dysfunction and a maladaptive immune response, and leads to chronic arterial wall inflammation. Numerous normal physiological activities exhibit daily rhythmicity, including energy metabolism, vascular function and inflammatory immunoreactions, and disrupted or misaligned circadian rhythms may promote the progression of atherosclerosis. However, the association between the circadian rhythm and atherosclerosis remains to be fully elucidated. In the present review, the effects of the circadian rhythm on atherosclerosis progression are discussed.
- Published
- 2020
38. Improving xylitol yield by deletion of endogenous xylitol-assimilating genes: a study of industrial Saccharomyces cerevisiae in fermentation of glucose and xylose
- Author
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Zi-Yuan Xia, Min Gou, Cai-Yun Xie, Bai-Xue Yang, Ya-Jing Wu, and Yue-Qin Tang
- Subjects
Saccharomyces cerevisiae ,Lignocellulosic biomass ,Xylose ,Xylitol ,Applied Microbiology and Biotechnology ,Microbiology ,Candida tropicalis ,chemistry.chemical_compound ,Aldehyde Reductase ,biology ,food and beverages ,General Medicine ,D-Xylulose Reductase ,biology.organism_classification ,carbohydrates (lipids) ,Phosphotransferases (Alcohol Group Acceptor) ,Glucose ,Biochemistry ,chemistry ,Metabolic Engineering ,Xylulokinase ,Fermentation ,Sorbitol ,CRISPR-Cas Systems ,Gene Deletion - Abstract
Engineered Saccharomyces cerevisiae can reduce xylose to xylitol. However, in S.cerevisiae, there are several endogenous enzymes including xylitol dehydrogenase encoded by XYL2, sorbitol dehydrogenases encoded by SOR1/SOR2 and xylulokinase encoded by XKS1 may lead to the assimilation of xylitol. In this study, to increase xylitol accumulation, these genes were separately deleted through CRISPR/Cas9 system. Their effects on xylitol yield of an industrial S. cerevisiae CK17 overexpressing Candida tropicalis XYL1 (encoding xylose reductase) were investigated. Deletion of SOR1/SOR2 or XKS1 increased the xylitol yield in both batch and fed-batch fermentation with different concentrations of glucose and xylose. The analysis of the transcription level of key genes in the mutants during fed-batch fermentation suggests that SOR1/SOR2 are more crucially responsible for xylitol oxidation than XYL2 under the genetic background of S.cerevisiae CK17. The deletion of XKS1 gene could also weaken SOR1/SOR2 expression, thereby increasing the xylitol accumulation. The XKS1-deleted strain CK17ΔXKS1 produced 46.17 g/L of xylitol and reached a xylitol yield of 0.92 g/g during simultaneous saccharification and fermentation (SSF) of pretreated corn stover slurry. Therefore, the deletion of XKS1 gene provides a promising strategy to meet the industrial demands for xylitol production from lignocellulosic biomass.
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- 2020
39. Spatio-temporally expressed sorbitol transporters cooperatively regulate sorbitol accumulation in pear fruit
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Kaijie Qi, Cai-Yun Yu, Chen Wu, Huping Zhang, Shaoling Zhang, Chao Gu, and Ruo-Fan Wu
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0106 biological sciences ,0301 basic medicine ,Sucrose ,Plant Science ,Fructose ,Biology ,01 natural sciences ,Pyrus ,03 medical and health sciences ,chemistry.chemical_compound ,Genetics ,Metabolome ,Sorbitol ,Food science ,Sugar ,Abscisic acid ,PEAR ,Gene Expression Profiling ,food and beverages ,Membrane Transport Proteins ,General Medicine ,Sweetness ,carbohydrates (lipids) ,Plant Leaves ,030104 developmental biology ,Glucose ,chemistry ,Fruit ,Transcriptome ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Sorbitol is the primary substrate translocated from source to sink in pear species. Among the many sorbitol transporters (SOTs), some are known to be involved in sorbitol accumulation in fruit; however, their particular roles are unclear. In this study, we examined the transcriptome and metabolome of a variety of pear samples from six time points to identify those SOTs. Similar to previous studies, sorbitol and sucrose differed significantly between the leaf and fruit, and sorbitol was consistently observed at higher concentrations at all time points. Interestingly, we found that sorbitol accumulation in pear fruit was cooperatively mediated by SOT3, SOT6/20, SOT19/21, and SOT22. In particular, the up-regulated SOT6/20 and SOT19/21 in fruit under 1 mg L-1 abscisic acid and 10 mg L-1 indole acetic acid treatments, respectively, resulted in an increased sorbitol concentration. In addition, sorbitol concentration showed positive correlations to fructose and glucose concentrations, indicating a role for sorbitol in the determination of fruit sweetness. Together with the deduced process of sugar biosynthesis, transport, conversion, and accumulation in pear, our study provides a foundation for further research into sugar accumulation processes in pear fruit, contributing to the improvement of fruit quality.
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- 2020
40. High serum lactate dehydrogenase and dyspnea: Positive predictors of adverse outcome in critical COVID-19 patients in Yichang
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Gongping Chen, Yongxu Jin, Ai-Guo Cheng, Cai-Yun Wang, Shuyu Zhang, Qingquan Chen, Xiaoting Lv, Qicai Liu, Yongping Zhu, and Haibo Ding
- Subjects
medicine.medical_specialty ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Adverse outcomes ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Gastroenterology ,Pulmonary fibrosis ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Retrospective Study ,Internal medicine ,Lactate dehydrogenase ,mental disorders ,Overall survival ,Medicine ,business.industry ,SARS-CoV-2 ,High serum ,COVID-19 ,General Medicine ,medicine.disease ,respiratory tract diseases ,Dyspnea ,chemistry ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,business - Abstract
BACKGROUND Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak in China, constitutes a Public Health Emergency of International Concern. It is well known that COVID-19 patients may have increased serum lactate dehydrogenase (LDH) levels in the early stage. The clinical changes in LDH may have predictive value in disease evolution and prognosis in critically ill COVID-19 patients. AIM To examine serum LDH and clinical characteristics in patients with COVID-19 and their predictive value for prognosis. METHODS This retrospective study analyzed the clinical data of forty-seven critical COVID-19 patients in the intensive care unit of the Third People's Hospital of Yichang City from January 27 to March 25, 2020 and divided them into survivors and non-survivors. The patients were diagnosed according to the World Health Organization interim guidance and critical cases met any one of the following criteria: Respiratory failure and required mechanical ventilation, the occurrence of shock, and the combined failure of other organs that required intensive care unit monitoring and treatments, according to the diagnostic criteria of critical COVID-19. Clinical data including symptoms, detection of SARS-CoV-2, chest computed tomography (CT) images, changes in serum LDH in different clinical phases, and prognosis were collected. Statistical analysis of the data was performed. Continuous variables were expressed as median (interquartile range) and compared with the Mann-Whitney U test. Categorical variables were compared with the Chi-square test. Survival data were analyzed using Kaplan-Meier survival curves and log-rank tests. RESULTS According to chest CT images, we observed the alveolitis and fibrosis stages in all critical patients in this study. Most non-survivors died in the fibrosis stage. Non-survivors had fewer days of hospitalization, shorter disease duration, shorter duration of alveolitis and fibrosis, and had dyspnea symptoms at disease onset (P = 0.05). Both first and lowest LDH values in the alveolitis stage were more pronounced in non-survivors than in survivors (449.0 U/L vs 288.0 U/L, P = 0.0243; 445.0 U/L vs 288.0 U/L, P = 0.0199, respectively), while the first, lowest and highest values of serum LDH in non-survivors were all significantly increased compared to survivors in the fibrosis phase (449.0 U/L vs 225.5 U/L, P = 0.0028; 432.0 U/L vs 191.0 U/L, P = 0.0007; 1303.0 U/L vs 263.5 U/L, P = 0.0001, respectively). The cut-off points of first LDH values in the alveolitis and fibrosis phase for distinction of non-survivors from survivors were 397.0 U/L and 263.0 U/L, respectively. In the fibrosis stage, non-survivors had more days with high LDH than survivors (7.0 d vs 0.0 d, P = 0.0002). Importantly, patients with high LDH had a significantly shorter median survival time than patients with low LDH in the alveolitis phase (22.0 d vs 36.5 d, P = 0.0002), while patients with high LDH also had a significantly shorter median survival time than patients with low LDH in the fibrosis phase (27.5 d vs 40.0 d, P = 0.0008). The proportion of non-survivors with detectable SARS-CoV-2 until death in the alveolitis stage was significantly increased compared with that in the fibrosis stage (100% vs 35.7%, P = 0.0220). CONCLUSION High LDH and dyspnea symptoms were positive predictors of an adverse outcome in critical COVID-19. The rapid progressive fibrosis stage was more perilous than the alveolitis stage, even if SARS-CoV-2 is undetectable.
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- 2020
41. Design, Preparation and Studies Regarding Cytotoxic Properties of Glycyrrhetinic Acid Derivatives
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Wim Dehaen, Rui Wang, Chang-Xin He, Qing-Xuan Zheng, Yan Xu, Cai-Yun Zhao, Zhi-Fang Wang, Hui-Jing Li, Qi-Yong Huai, and Rui Zhang
- Subjects
0301 basic medicine ,glycyrrhetinic acid ,Cell Survival ,MDA-MB-231 ,Pharmaceutical Science ,Antineoplastic Agents ,Apoptosis ,Pharmacology ,HeLa ,03 medical and health sciences ,chemistry.chemical_compound ,Structure-Activity Relationship ,0302 clinical medicine ,Bromide ,Cell Line, Tumor ,medicine ,Cytotoxic T cell ,Humans ,Doxorubicin ,Pharmacology & Pharmacy ,BIOLOGICAL EVALUATION ,Cytotoxicity ,Science & Technology ,Natural product ,biology ,Chemistry ,apoptosis ,Gefitinib ,General Medicine ,biology.organism_classification ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer cell ,cytotoxicity ,Glycyrrhetinic Acid ,Life Sciences & Biomedicine ,medicine.drug - Abstract
Glycyrrhetinic acid (GA) is a natural product with certain antitumor activity. In order to enhance the cytotoxicity, a total of eighteen derivatives of GA were designed and synthesized. Their cytotoxicity against MDA-MB-231cells (human breast cancer cells) and HeLa cells (human cervical cancer cells), were evaluated by the MTT method (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide). The results indicated that these target compounds have a wide molar activity range and some of them show better activity than the commercial drugs gefitinib and doxorubicin. Compound 6g induces apoptosis of 7, 10 and 44% of MDA-MB-231 cells at 5, 10, and 20 µM, respectively. ispartof: BIOLOGICAL & PHARMACEUTICAL BULLETIN vol:43 issue:1 pages:102-109 ispartof: location:Japan status: published
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- 2020
42. The impact of major dietary patterns on glycemic control, cardiovascular risk factors, and weight loss in patients with type 2 diabetes: A network meta-analysis
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Long Ge, Qingxia Yang, Cai-yun Gao, Jinhui Tian, Yiting Wu, Yang-qin Xun, Bei Pan, and Guowu Ding
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Blood Glucose ,medicine.medical_specialty ,Waist ,Mediterranean diet ,Network Meta-Analysis ,Cardiovascular risk factors ,Type 2 diabetes ,Diet, Mediterranean ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,Risk Factors ,law ,Weight loss ,Internal medicine ,Weight Loss ,medicine ,Humans ,030212 general & internal medicine ,Triglycerides ,Randomized Controlled Trials as Topic ,Glycemic ,Glycated Hemoglobin ,business.industry ,Health Policy ,Cholesterol, HDL ,General Medicine ,medicine.disease ,Dietary Fats ,Diabetes Mellitus, Type 2 ,Cardiovascular Diseases ,Meta-analysis ,Waist Circumference ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
OBJECTIVE To comprehensively compare the differences between major dietary patterns in improving glycemic control, cardiovascular risk, and weight loss for patients with type 2 diabetes. METHOD We systematically searched PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials (CENTRAL) to identify randomized controlled trials (RCTs) that compared the efficacy of Mediterranean, low-carbohydrate, and/or low-fat dietary patterns for patients with type 2 diabetes. Studies that compared any one of those diets with regular diet were also included. The risk of bias in eligible RCTs was evaluated according to the Cochrane Handbook. The primary outcome was glycemic control. Secondary outcomes included weight loss and cardiovascular risk factors. A network meta-analysis was performed using R-3.3.2. RESULTS Ten RCTs involving five dietary patterns met the eligibility criteria. The findings revealed that compared to low-fat diet, Mediterranean diet showed beneficial effects in glycemic control (HbA1c [%]: MD = -0.45, 95% CI = -0.55 to -0.34; fasting plasma glucose [mmol/L]: MD = -1.24 95% CI = -1.57 to -0.91, respectively; weight loss [kg]: MD =-1.18, 95% CI = -1.99 to -0.37; waist circumference [cm]: MD = -0.73, 95% CI = -1.26 to -0.19), and cardiovascular risk factors (HDL-cholesterol [mmol/L]: MD = 0.07, 95% CI = 0.04 to 0.11; total cholesterol [mmol/L]: MD = -0.17, 95% CI = -0.26 to -0.08; triglycerides [mmol/L]: MD = -0.21, 95% CI = -0.27 to -0.16). Network meta-analysis showed that high-carbohydrate diet improved HDL-cholesterol (mmol/l) (MD = 1.04, 95% CI = 0.39 to 1.68) when compared with regular diet. The differences were not statistically significant in other indirect comparison groups for other outcomes. CONCLUSIONS A mediterranean diet showed beneficial improvements in glycemic control, weight loss, and cardiovascular risk factors in people with type 2 diabetes.
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- 2018
43. Effect of
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Xiao-Bo Wu, Xiao-Ping Han, Li Longxue, Tao Hong, Cai-Yun Dan, Liu Zhiyong, and Jing-Liang Shi
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0301 basic medicine ,Article Subject ,Longevity ,Biology ,complex mixtures ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Honey Bees ,0302 clinical medicine ,Animal science ,Memory ,Memory formation ,Animals ,Learning ,Sucrose syrup ,General Immunology and Microbiology ,Low dose ,fungi ,General Medicine ,Astragalus propinquus ,Bees ,biology.organism_classification ,Astragalus ,030104 developmental biology ,behavior and behavior mechanisms ,Medicine ,030217 neurology & neurosurgery ,Research Article - Abstract
In this study, the effects of Astragalus membranaceus oral solution on lifespan and learning and memory abilities of honey bees were evaluated. Two groups of bees were fed with sucrose syrup (50%) containing low dose (1.33%) and high dose (13.3%) of A. membranaceus oral solution, respectively. The proboscis extension response (PER) analysis was applied to examine the learning and memory capabilities of bees. Two genes related to memory formation in honey bees were determined by real-time PCR. High dose (13.3%) of A. membranaceus significantly decreased the mean lifespan of bees compared to the bees fed with low dose (1.33%) and control bees. No significant differences in lifespan of bees were found between low-dose-fed bees and control bees. The results of PER experiments showed apparent improvement in the memorizing ability of the high-dose group (in comparison with the control group). Moreover, the relative expression levels of Nmdar1 in the low-dose group and control group were significantly lower than those in the high-dose group. It is preliminarily concluded that A. membranaceus has an adverse effect on the mean lifespan of honey bees but might be helpful in strengthening memories.
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- 2019
44. Protective effects of 3β-angeloyloxy-8β, 10β-dihydroxyeremophila-7(11)-en-12, 8α-lactone on paraquat-induced oxidative injury in SH-SY5Y cells
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Jie Qi, Cai-Yun Zhang, Qi-Lin Wang, Xia Li, Wei-Dong Xie, Fang-Yuan Liu, Jing-Jing Zhuang, Chao Guo, Jia-Hui Ma, and Shi-Qi Lin
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Paraquat ,SH-SY5Y ,Cytochrome ,Pharmaceutical Science ,Apoptosis ,Pharmacology ,medicine.disease_cause ,01 natural sciences ,Analytical Chemistry ,Lactones ,chemistry.chemical_compound ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,Oxidative injury ,chemistry.chemical_classification ,biology ,Caspase 3 ,Superoxide Dismutase ,010405 organic chemistry ,Organic Chemistry ,Neurodegeneration ,Cytochromes c ,General Medicine ,Glutathione ,medicine.disease ,Caspase 9 ,Mitochondria ,0104 chemical sciences ,Oxidative Stress ,010404 medicinal & biomolecular chemistry ,Complementary and alternative medicine ,chemistry ,biology.protein ,Molecular Medicine ,Reactive Oxygen Species ,Sesquiterpenes ,Lactone ,Oxidative stress - Abstract
3β-Angeloyloxy-8β,10β-dihydroxyeremophila-7(11)-en-12,8α-lactone (FJ1) inhibited effectively paraquat (PQ)-induced injury in SH-SY5Y cells. In this way, FJ1 was shown to reverse the PQ-induced activation of caspase-9 and caspase-3, the increase in Bax/Bcl-2 ratio, and the release of cytochrome c. The mechanism was associated with a reduction of oxidative stress, including the decrease in the levels of ROS and MDA and maintaining the activity of SOD and GSH. Taken together, findings revealed that FJ1 had protective effects against PQ-induced injury via attenuating the oxidative stress in SH-SY5Y cells, which suggested that FJ1 might be a candidate for further evaluation against neurodegeneration in Parkinson's disease.
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- 2018
45. Change of Inflammatory Factors in Patients with Acute Coronary Syndrome
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Cai-Yun Ma, Zhen-Ye Xu, Jinghua Liu, Hongyu Peng, Feng-Xue Ren, Fang Liu, and Shao-Ping Wang
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Adult ,medicine.medical_specialty ,Acute coronary syndrome ,Heart disease ,lcsh:Medicine ,Coronary Disease ,030204 cardiovascular system & hematology ,Coronary Angiography ,Chest pain ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Coronary Heart Disease ,Clinical significance ,Chitinase-3-Like Protein 1 ,030212 general & internal medicine ,Acute Coronary Syndrome ,Aged ,Intracellular Adhesion Molecule-1 ,medicine.diagnostic_test ,business.industry ,Lipoprotein-associated phospholipase A2 ,lcsh:R ,General Medicine ,Middle Aged ,Intercellular Adhesion Molecule-1 ,medicine.disease ,Thrombosis ,Lipoprotein-Associated Phospholipase A2 ,Stenosis ,1-Alkyl-2-acetylglycerophosphocholine Esterase ,Angiography ,Cardiology ,Original Article ,medicine.symptom ,business - Abstract
Background: Acute coronary syndrome (ACS) is closely related to unstable plaques and secondary thrombosis. The inflammatory cells in plaques and their inflammatory products may be the cause for plaque instability and ruptures. The study aimed to disclose the changes of inflammatory factors including serum intracellular adhesion molecule-1 (ICAM-1), chitinase-3-like protein 1 (YKL-40), and lipoprotein-associated phospholipase A2 (Lp-PLA2) in patients with ACS and its clinical significance. Methods: A total of 120 patients with coronary heart disease (CHD) were categorized into 2 groups: 69 with ACS and 51 with stable angina pectoris (SAP); 20 patients with chest pain and normal angiography served as a control group. The 120 patients with CHD were categorized into single-vessel disease group, double-vessel disease group, and three-vessel disease group based on the number of coronary artery stenosis. The severity of coronary artery stenosis was quantified based on coronary angiography using Gensini score. They were further divided into mild CHD group with its Gensini score 54 (n = 36). Serum levels of ICAM-1, YKL-40, and Lp-PLA2 of different groups were determined by enzyme-linked immunosorbent assay. Correlation between ICAM-1, YKL-40, Lp-PLA2, and Gensini score was analyzed. Results: The levels of serum inflammatory factors ICAM-1, YKL-40, and Lp-PLA2 were significantly higher in the ACS group than those in control group and SAP group (all P < 0.05); and compared with control group, no significant difference was observed in terms of the serum ICAM-1, YKL-40, and Lp-PLA2 levels in the SAP group (P > 0.05).The levels of serum ICAM-1, YKL-40, and Lp-PLA2 were not significantly different among control group, single-vessel disease group, double-vessel disease group, and three-vessel disease group (all P > 0.05). The levels of serum ICAM-1, YKL-40, and Lp-PLA2 were not significantly different among control group, mild CHD group (Gensini score 54) (all P > 0.05). Nonparametric Spearman correlation analysis showed that the levels of serum ICAM-1, YKL-40, and Lp-PLA2 were not correlated with the Gensini score in CHD patients (r = 0.093, r = –0.149, and r = –0.085, all P > 0.05; respectively). Conclusions: The serum levels of ICAM-1, YKL-40, and Lp-PLA2 were correlated with different clinical types of CHD, but not well correlated the severity and extent of artery stenosis, suggesting that ICAM-1, YKL-40, and Lp-PLA2 might be involved in occurrence of instability of atherosclerotic plaque, and might reflect the severity of CHD mostly through reflecting the plaque stability.
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- 2018
46. SPP1 rs4754 and its epistatic interactions with SPARC polymorphisms in gastric cancer susceptibility
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Le Zong Chen, Feng Wang, Dong Liang Chen, Jian Yong Shao, Jun Ling Peng, D Yang, Zu-Lu Ye, Xuan Su, Rui-Hua Xu, Cai Yun He, and Zi Xian Wang
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Male ,0301 basic medicine ,Single-nucleotide polymorphism ,Biology ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Stomach Neoplasms ,Genotype ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,Osteonectin ,Gene ,Cancer ,Epistasis, Genetic ,General Medicine ,Middle Aged ,medicine.disease ,Phenotype ,Genotype frequency ,030104 developmental biology ,Case-Control Studies ,030220 oncology & carcinogenesis ,Cancer research ,Epistasis ,Female ,Osteopontin ,Variants of PCR - Abstract
The matricellular glycoprotein products of the SPP1 and SPARC genes play critical roles in many aggressive tumor phenotypes including gastric cancer. We sought to test whether the polymorphisms of these two genes, individually or jointly, influence gastric cancer susceptibility. Nine potentially functional, tagging single nucleotide polymorphisms (tagSNPs) of SPP1 and SPARC were selected and detected using the Kompetitive Allele Specific PCR method in 301 gastric cancer cases and 1441 healthy control subjects. We found that the genotype frequencies of SPP1 rs4754 in gastric cancer were significantly different from those in controls. The rs4754 TT genotype conferred an increased risk of gastric cancer, with unadjusted and adjusted ORs ranging from 1.75 to 1.95 (all P0.05). The assessment of the effect modifications of sex and age on the genetic effects also confirmed the statistically significant association of the rs4754 TT genotype with increased gastric cancer risk. Epistatic interactions were found between SPP1 rs4754 and SPARC rs1054204, rs3210714 and rs3549 (all P values for interaction0.05). During the assessment of the epistatic effects between pairs of interacting factors, increased gastric cancer risk was observed in the combined presence of the SPP1 rs4754 TT genotype and the common genotypes of interacting SPARC SNPs, with ORs ranging from 3.94 to 4.41. When the genetic influence of SPP1 rs4754 TT was excluded, the genetic effects of the SPARC rs1054204, rs3210714 and rs3549 common genotypes on gastric cancer susceptibility switched from being risky to beneficial. These data reveal an association between the SPP1 rs4754 polymorphism and altered risk of gastric cancer and highlight an important role of the epistatic effects of SPP rs4754 with SPARC polymorphisms in gastric carcinogenesis. Additional functional experiments and independent large-scale studies, especially in other ethnic populations, are needed to confirm our results.
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- 2018
47. Associations between matrix metalloproteinase gene polymorphisms and glaucoma susceptibility: a meta-analysis
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Chun-Yan Deng, Le Peng, Ming-Yue Wu, Lan Zhou, Cai-Yun Liu, Yang Wu, and Yong Zhang
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,Genotype ,Population ,Protective factor ,Glaucoma ,Subgroup analysis ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,lcsh:Ophthalmology ,Risk Factors ,Internal medicine ,Ophthalmology ,medicine ,Humans ,Genetic Predisposition to Disease ,Polymorphism ,education ,education.field_of_study ,business.industry ,General Medicine ,Odds ratio ,Publication bias ,medicine.disease ,Confidence interval ,030104 developmental biology ,Matrix metalloproteinases ,lcsh:RE1-994 ,Meta-analysis ,030221 ophthalmology & optometry ,business ,Research Article - Abstract
Background Matrix metalloproteinases (MMPs) polymorphisms have been implicated in the pathogenesis of glaucoma risk. However, the results were controversial. We performed a meta-analysis to evaluate the precise associations between MMPs polymorphisms and glaucoma risk. Methods Related studies were reviewed by searching electronic databases within four databases. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the association between the most common polymorphisms of MMPs and glaucoma risk. Heterogeneity, publication bias and sensitivity analysis were conducted to guarantee the statistical power. Results Overall, 11 selected articles involving 2,388 cases and 2,319 controls were included in this meta-analysis. Significant associations were only found between MMP-9 rs17576 G > A polymorphism (GA vs. GG: OR = 0.80, 95%CI = 0.67-0.97, P = 0.02, I2 = 0%), MMP-9 rs3918249 C > T polymorphism (TT vs. CC + CT: OR = 0.71, 95%CI = 0.51-0.98, P = 0.04, I2 = 0%) and glaucoma risk in the general population. Subgroup analysis also suggested that MMP-9 rs17576 G > A was related to glaucoma in the Caucasian population (GA vs. GG: OR = 0.67, 95%CI = 0.45-1.00, P = 0.05; GA + AA vs. GG: OR = 0.66, 95%CI = 0.45-0.97, P = 0.03, I2 = 0%). Conclusions Our meta-analysis demonstrates that MMP-9 rs17576 G > A polymorphism might be a protective factor against the development of glaucoma in Caucasian population.
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- 2017
48. The Novel Nature Microtubule Inhibitor Ivalin Induces G2/M Arrest and Apoptosis in Human Hepatocellular Carcinoma SMMC-7721 Cells In Vitro
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Fang-Yuan Liu, Cai-Yun Zhang, Zhuo Han, Wei-Dong Xie, Shi-Qi Lin, Fu-Juan Jia, Xia Li, and Jia-Hui Ma
- Subjects
Cell cycle checkpoint ,Carcinoma, Hepatocellular ,carpesium divaricatum ,Microtubules ,Article ,03 medical and health sciences ,Lactones ,0302 clinical medicine ,Cell Line, Tumor ,Humans ,Ivalin ,HCC ,microtubule ,cell cycle arrest ,apoptosis ,Cyclin B1 ,Mitosis ,030304 developmental biology ,Cell Proliferation ,0303 health sciences ,Cyclin-dependent kinase 1 ,lcsh:R5-920 ,biology ,Chemistry ,Cell growth ,Liver Neoplasms ,General Medicine ,Cell cycle ,Cell biology ,G2 Phase Cell Cycle Checkpoints ,Tubulin ,030220 oncology & carcinogenesis ,Cancer cell ,biology.protein ,lcsh:Medicine (General) ,Sesquiterpenes - Abstract
Background and Objectives: Microtubules are an attractive target for cancer chemotherapy. Previously, we reported that Ivalin exhibited excellent anti-migration and anti-invasion activities in human breast cancer cells. Here, we examined the microtubule inhibition effect of Ivalin in human hepatocellular carcinoma SMMC-7721 cells. Materials and Methods: We used the 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay to evaluate the cell proliferation effect of Ivalin and flow cytometry analysis to detect the apoptotic and cell cycle arrest effects of Ivalin. Immunofluorescence staining was used to measure the effect of Ivalin on the cytoskeleton network, and Western blotting was used to detect the expression levels of Bax, Bcl-2, Cdc2, phosphor-Cdc2, Cdc25A, Cyclin B1, and tubulin. Results: Ivalin induced cell cycle G2/M arrest and subsequent triggered apoptosis in human hepatocellular carcinoma SMMC-7721 cells. Furthermore, microtubules were shown to be involved in Ivalin-meditated apoptosis. In this connection, Ivalin treatment suppressed cellular microtubule network formation by regulating microtubule depolymerization. Moreover, Western blotting revealed Cdc25A and Cyclin B1 were upregulated in Ivalin-meditated cell cycle arrest. Subsequently, the induction of Bax (a proapoptotic protein) and reduction of Bcl-2 (an anti-apoptotic protein) expression were observed in Ivalin-treated SMMC-7721 cells. Conclusion: Ivalin induced microtubule depolymerization, then blocked cells in mitotic phase, and eventually resulted in apoptosis in SMMC-7721 cells. Collectively, these data indicate that Ivalin, acting as a novel inhibitor of microtubules, could be considered as a promising lead in anticancer drug development.
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- 2019
49. Assessment of two different HER2 scoring systems and clinical relevance for colorectal cancer
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Shaoyan Xi, Ying Jin, Cai-Yun He, Feng Wang, Rui-Hua Xu, Zi-Xian Wang, Chao Ren, Fu-rong Liu, and Fang Wang
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Oncology ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,Colorectal cancer ,Receptor, ErbB-2 ,Pathology and Forensic Medicine ,Young Adult ,Internal medicine ,HER2 ,Biomarkers, Tumor ,Medicine ,Humans ,In patient ,Clinical significance ,Stage (cooking) ,skin and connective tissue diseases ,Molecular Biology ,Human Epidermal Growth Factor Receptor 2 ,neoplasms ,Aged ,Aged, 80 and over ,Gastroesophageal adenocarcinoma ,business.industry ,Significant difference ,Methodology ,Cell Biology ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Primary tumor ,digestive system diseases ,Female ,Original Article ,business ,Colorectal Neoplasms - Abstract
Although the positivity of human epidermal growth factor receptor 2 (HER2) is low in colorectal cancer (CRC), anti-HER2 is becoming a new target therapy in metastatic colorectal cancer (mCRC). However, assessment of the HER2 scoring system was still not established in CRC. The purpose of our study was to evaluate HER2 status and its correlation with clinicopathological characteristics and survival according to the HER2 diagnostic criteria for gastroesophageal adenocarcinoma (GEA criteria) and the HERACLES diagnostic criteria (HERACLES criteria) in a large cohort of Chinese CRC patients. The HER2 positivity was 2.9% (43/1490) and 2.6% (39/1490) in CRCs based on the GEA criteria and the HERACLES criteria, and 3.7% (9/243) in mCRC according to both criteria. HER2 status was associated with primary tumor location (P = 0.037), regional lymph node metastasis (P = 0.035), and TNM stage (P = 0.022) in CRCs based on the HERACLES criteria. No such association was found based on the GEA criteria. Furthermore, HER2 positive only presented in patients with RAS gene wild type (P = 0.001). Significant difference was only observed between the HER2-positive and HER2-negative groups in terms of disease-free survival for stage II-III CRCs (P = 0.048) according to the HERACLES criteria, but not based on the GEA criteria. Our findings suggest that the frequency of HER2 overexpression or amplification was low in Chinese CRC patients, and provide a rationale for further evaluation of HER2 in CRC based on the HERACLES criteria and the HER2 diagnostic criteria for gastroesophageal adenocarcinoma.
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- 2019
50. Effects of volatile anesthetic preconditioning on expression of NFκB-regulated genes in aged rat myocardium
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Hong Qiu, Jun Chen, Hong Liu, and Cai-Yun Zhong
- Subjects
Aging ,Medical Biotechnology ,Diastole ,Ischemia ,030204 cardiovascular system & hematology ,Pharmacology ,Cardiovascular ,Caspase 8 ,General Biochemistry, Genetics and Molecular Biology ,Sevoflurane ,03 medical and health sciences ,0302 clinical medicine ,030202 anesthesiology ,Medicine ,Heart Disease - Coronary Heart Disease ,Caspase-9 ,Cardioprotection ,biology ,business.industry ,General Medicine ,medicine.disease ,Preload ,Heart Disease ,Anesthetic ,biology.protein ,Original Article ,business ,medicine.drug - Abstract
Volatile anesthetic preconditioning has been shown to be a potent way to provide myocardium protection against ischemia/reperfusion (I/R) injury; however, this cardioprotection is lost in senescent animal models and elderly patients. NFkB-regulated genes have been linked to myocardial I/R injury and anesthetic preconditioning. Here, we investigated NFkB activation related to anesthetic preconditioning in aging rat myocardium. Isolated, Langendorff perfused rat hearts from Fischer 344 male rats, 24 months old, were randomly assigned to one of the three groups. The hearts of the control group were perfused with physiologic solution without any intervention. The hearts in the I/R group were subjected to 25 minutes ischemia and followed by 60 minutes reperfusion. The hearts in the treatment group were subjected to 10 minutes 2.5% sevoflurane, followed by 20 minutes washout and by 25 minutes ischemia and 60 minutes of reperfusion, respectively. Left ventricular developed pressure (LVDP) and left ventricular end-diastolic pressure (LVEDP) were measured. Western blot analysis was used to measure inhibitor of kB (IkB) and anti-apoptotic genes: A1, ILP, c-IAP-2, Bcl-2, caspase 8 and caspase 9. Ischemia and reperfusion significantly decreased LVDP and increased LVEDP in aged rat hearts. Anesthetic preconditioning with sevoflurane did not change the effects I/R on LVDP and LVEDP, despite the fact that after treatment with anesthetic preconditioning, the levels of IκB, A1, ILP, caspase 8 and caspase 9 were significantly different compared to those of the control hearts. In conclusion, anesthetic preconditioning with sevoflurane does not improve myocardial systolic and diastolic functions. Our results suggest that the activation of NFkB regulated genes is different in the senescent myocardium and could account for loss of cardioprotection with aging.
- Published
- 2019
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