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Soy protein isolate (SPI) and sugar beet pectin (SBP) were adopted to fabricate double network (DN) gels via thermal treatment and laccase-catalysis. The concentrations of SBP (0.5%-2.5%, w/w) and SPI (4%-8%, w/w) were evaluated. DN gels showed higher holding water capacity (WHC, above 83%), compared with SBP single gel (75.96%). The presence of SPI improved the mechanical properties of gels significantly. Apparent phase separation could be observed when SPI concentration was 4%. Moreover, interpenetrating networks gradually formed with the increase of SPI concentration. The favorable structural heterogeneity and mechanical integrity derived from these polymers might be mainly responsible for the enhancement of the mechanical properties. The presence of SBP and laccase could improve the β-sheet amounts of SPI and make it form more rigid structure according to the results of circular dichroism (CD) and fluorescence spectra. The excellent performance of DN gels could enable the delivery of various components.

In this study the effect of fucoidan SJP-3 extracted from Stichopus Japonicus was used to evaluate the protective effect on PC12 cells with hypoxia/reoxygenation injury. SJP-3 can stabilize the mitochondrial membrane potential and resistant Cyt-C/mitochondrial apoptotic pathway via increasing the Bcl-2 expression and decreasing expression of the Bax, caspase-3, caspase-9 suggesting that SJP-3 may exert a protective effects on neural cells with hypoxia/reoxygenation injury through the protection of mitochondria, the stability of mitochondrial membrane potential and inhibition of mitochondrial apoptosis pathway.

With the rapid development of modern electromagnetics, a lot of electromagnetic field numerical methods are maturing. 3D electromagnetic simulation software HFSS has two solutions including incentive solving and eigenmode solving. When solving the calculation of the structure of eigenmodes or resonant type can use eigenmode solver. Using matlab generate HFSS scripting language-based program to set eigenmode solution is a very convenient and very innovative approach. The program on a circular cavity modeling and eigenmode solution set simulation results meet the design requirements.

The DOA Estimation of the MIMO radar is widely used to determine the position of the objects. Having been considered the multipath communication environment, this paper estimates the DOA information of low elevation targets using beam space MUSIC algorithm based on the model of uniform linear array of MIMO radar. Simulations have shown that the algorithm is effective in the multipath spread environment, and the resolution of the DOA estimation of angular is about 0.5°

A series of electrical parameters is used to describe the performance of antenna. Antenna pattern which is the main electrical parameter of antenna shows an aerial image of radiation field. The antenna pattern simulation system which can make antenna users install the antenna visual is developed in Visual Studio 2005 with the application of MFC programming technology. Test results show that the antenna pattern simulation system is effective.

Two-factor and three-level fractional factorial design was employed for evaluation of the effect of Glycine and Triton X-100 on the secretion and expression of ZZ-EGFP fusion proteins. Varying contents of glycine (0%, 1%, 2%) and Triton X-100 (0%, 1%, 2%) were added into shaking flasks, respectively, and supplied with appropriate volume of ampicillin (total 9 combinations; group at concentration zero serving as control) to promote more ZZ-EGFP diffuse into liquid culture medium. Fluorescent intensity in the culture supernatant was detected. A standard curve could be generated on the basis of fluorescent intensity and protein concentration. The expression level of ZZ-EGFP fusion proteins was estimated by checking the protein standard curve concentration fluorescene intensity. Results show that when the culture medium contains 2% Glycine and 1% Triton X-100, the expression level of ZZ-EGFP was able to be greatly increased. Further experiments revealed that absorbance value (A600) in the experiment group, whose culture medium contains 2% Glycine and 2% Triton X-100, is significantly lower than other groups in the present experiment. These results indicate that the culture medium containing appropriate quantity of Glycine and Triton X-100 is favourable to the secretion and expression level of ZZ-EGFP in gene-engineering bacteria Escherichia coli HB101.

Aim: To study the relationship between primary structures of oligodeoxynucleo-tides (ODN) containing unmethylated deoxycytidyl-deoxyguanosine (CpG) dinucleotide motifs and their immunostimulatory activities in mouse spleen cells. Methods: A series of CpG ODN with different primary structures were synthesized. Their capabilities to stimulate mouse spleen cell proliferation were determined by [3H]thymidine incorporation assay. Cytokine (interleukin [IL]-6, IL-12, and IFN-α) secretion spectra induced by CpG ODN were assessed by ELISA. The ability of CpG ODN to activate natural killer cells was evaluated by standard 4 h 51Cr-release assay. Flow cytometry was utilized to examine the expressions of various lymphocyte surface molecules on diverse immunocytes. An effective CpG ODN for murine, ODN1826, was set as the template of modification and the positive control. Results: The immunostimulatory activities of CpG ODN with different sequences and compositions varied markedly, both in character and in extent. It was useless for improving the immunostimulatory activity of ODN1826 by simply increasing the functional hexameric CpG motif number, modifying the site of CpG motifs, or changing the distance between multi-CpG motifs. However, an addition of a self-complementary palindrome structure at the 3′-end, but not the 5′-end of CpG ODN, aroused marked improvement in its activity. Several designed ODN had superior comprehensive immunostimulatory properties compared to ODN1826. Conclusion: The immunostimulatory activity of a CpG ODN was relevant to its primary structure. It was useless for promoting immunostimulatory activity to simply change CpG motif number, space, or distance. The 3′-end palindrome structure of CpG ODN is associated with enhanced immunostimulatory activity.