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Purpose. Due to changing climatic conditions and the spread of new viral diseases, an important issue in beet growing is the expansion of plasma ulture using new sterile cytoplasm from wild species of the genus Beta L. as an alternative to Owen’s S-cytoplasm. The goal of this work was to obtain new breeding genotypes based on new cytoplasm. Methods. Cytological, biotechnological. field and laboratory. Results. Availability of recombination in the locus of grafted genes of one-year and two-year development cycles B+b- and hypocotyl colour R+r- was determined using the method of analytical crosses. Earlier we have proved the positive effect of sterility maintainer Beta vulgaris Nxxzz in replacement of the sterility maintainer genome based on our results on the selection of hypocotyl of green color r-rand two-year development cycle b-b- in the interspecific hybrids F2 obtained under the hybridization scheme Beta maritima L: ♀B1C maritima / ♂ Beta vulgaris Nxxzz (Роїк М. В., 2013). In the case of interspecific hybrids, in the conditions of sterile cytoplasm Beta patula L., only backcross offspring of the third cycle of saturating crosses were identified by one- and two-year developmental cycles. Seed bearing plants of BC3S patula of a two-year development cycle were characterized by spreading stems, multi-seeded fruits (3, 4 seeds) and sterile anthers. At the same time, monogermity and a two-year development cycle were restored against the background of sterile cytoplasm of Beta patula L. only among the seed bearing plants of the fourth cycle of saturating crosses. Recombinant genotypes with a red hypocotyl colour and a two-year development cycle BC4S patula R+r- were characterized by multigermity, chimericalness by sterility of CMS‑1 and CMS‑0 type and single- and multi-stem morphological types of anthers. The expression of CMS nuclear genes in the newly replaced BC5S patula lines was marked by high sterility rates of the CMS‑0 type; however, the fertility rates required additional rejection of multigerm seed plants. This is an indicator of the complex structure of the fertility locus in wild Beta patula L. and the special response of the nuclear genome of the sterility maintainers to the new cytoplasmic background. Experimental triploid hybrids with the new introductory sterile cytoplasm Beta patula L. were determined by high sugar content and dry matter content up to 29%. Conclusions. Alloplasmic lines based on the cytoplasm of the wild species Beta patula L are created. Their sugar content at the level of experimental triploid hybrids was 19.5% and 20.5% with a dry matter content of 25% —29%. Alloplasmic lines with a new BCC Beta patula L. recommended for use in the selection process of sugar beets.

Introduction. Today, alternative natural resources are increasingly used as the main industrial raw material used not only for the production of biofuels and various types of energy, but also other products. Bioenergy crops of miscanthus and switchgrass, which are characterized by long-term high yields, in contrast to, for example, agricultural waste, can be a promising way to attract new sources of high quality cellulose and for the production of bioplastics. The latest scientific research of miscanthus confirms the hypothesis of Ukrainian scientists of the 1980s and scientists from other countries that the raw material of this cellulose-containing crop is fully positioned as a material for the production of cellulose and bioplastics, and for biotechnological production of soluble carbohydrates and biofuels. Conclusion. It is proved that bioenergy crops of miscanthus and switchgrass can be a promising way to attract new sources of high quality cellulose for bioplastics production.

Goal. Expanding the breeding trait collection of breeding genotypes of the genus Miscanthus (Anderson) and the genetic basis for new allotriploid clones by transferring components for hybridization of natural wild species Miscanthus sinensis and Miscanthus sacchariflorus to the tetraploid level. Methods. Cytological, biotechnological, fluorescent cytphotometry, field, laboratory. Results. The efficiency of polyploidisation for induction of new tetraploid forms of miscanthus in liquid nutrient media supplemented with colchicine (0.05% mass) and for stabilization of myxoploid shoots (0.005% mass) for 6 h of cultivation is examined. The period of exposure for Miscanthus sinensis (2x=2x= 38) and Miscanthus sacchariflorus (2x = 2x = 38) varied from 2 hours to 3 days depending on the genetic origin of the material, with transfer to a hormonal environment. The best indicators of tetraploid induction for Miscanthus sinensis were observed for the exposure period of 2 days with polyploidization efficiency (Db%) of 31.25% and 21.42%, and for Miscanthus sacchariflorus 2 and 6 hours with 35.0% and 27.3%, respectively. The technology of transfering rooted shoots into the soil on the Yaltushkiv Eperimental Breeding Farm was improved and the composition of the soil mixture that provided 99% rooting of culture seedlings at a humidity of 60−70% and air temperature of 35−55°С was found. Conclusions. New biotechnological tetraploid lines of Miscanthus sinensis (2xn=4x=76) and Miscanthus sacchariflorus (2xn=4x=76) were created in the conditions of liquid nutrient media supplemented with colchicine (0.05 % mass). It is investigated that flowering of new tetraploid clones in the conditions of Ukraine for the second year of vegetation occurs from late September to early October with formation of fertile pollen grains.

Protective forest plantations are an important part of forest-agricultural landscapes and one of the most effective long-term and relatively inexpensive measures to combat wind and water soil erosion. They have a positive effect on the microclimate of the surrounding areas and can significantly increase crop yields. Also, under certain conditions, they can become an important source of bioenergy feedstock. Goal. Development of theoretical bases and practical measures of the use of agroforestry reclamation plantations of Ukraine for energy purposes. Methods. In the course of the research it was envisaged to develop schemes of protective forest plantations on the basis of the analysis of the existing information, long-term production and scientific experience which, at full performance of ecological functions, can be used for obtaining bioenergy feedstock. Results. It is established that for the simultaneous use of protective forest plantations as a source of energy biomass it is necessary to include fast-growing woody plants (willow, poplar, acacia, maple, etc.) in rows or backstage, which are expected to be periodically cut for biomass. Such plants are then intensively restored by growth from stumps. The basis of such plantations for long-term and permanent performance of their protective functions should mainly consists of the rows of oak trees. Conclusions. Protective forest plantations, in addition to the positive impact on the microclimate of the environment and a significant increase in the productivity of agricultural land, can also play an important role as a source of bioenergy feedstock. Today, forest protection plantations are losing their functions for various reasons and need to be reconstructed. At the same time, along with the restoration of protective plantations, it will be possible to obtain about 54.6 million tons of wood for energy needs and form new protective plantations, which, along with improving the ecological environment, will create a reliable feedstock base for bioenergy. The schemes presented in the article can be applied at the stage of restoration and expansion of the field protection system to increase their energy role.

It was found that long-term cultivation of perennial cereal bioenergy plants has a positive effect on the fertility of low-yielding soils. Thus, for the cultivation of switchgrass for nine years, the content of organic carbon in the soil increased from 1.87 to 2.40%, and for the cultivation of giant miscanthus for six years from 1.87 to 2.42%. Conclusions. Laying plantations of perennial cereal bioenergy plants on low-yielding and erosion-prone lands helps to restore their fertility and provides a sustainable supply of high quality feedstock for the production of biofuels.

Purpose.The analysis of the agroecological state of agricultural lands of Ukraine and the factors that cause soil degradation are given in the article. Conclusions. Large-scale use of straw for energy may be one of the key factors in reducing organic carbon in the soil. This will activate the processes of humus mineralization. Therefore, the use of straw for energy production does not meet the criteria of sustainable development and violates Ukrainian legislation on land use. The sustainable bioenergy development must be based on the biomass of high-productive bioenergy plants, which should be grown on low-yielding and degraded (marginal) land.

Purpose. Investigation of cytogenetic aspects of embryological processes in the culture of immature apomictic embryos, breeding genotypes of sugar beet with cytoplasmic sterility for differentiation and selection by gametophyte reduced parthenogenesis. Methods. Cytological, biotechnological, fluorescent cytophotometry, field, laboratory. Results. The cytogenetic features of genesis of immature apomictic embryos cells induced in vitro on the 12th, 20th and 22th days of development have been investigated on the basis of CMS apozygotic lines of Beta vulgaris and alloplasmic lines of wild species Beta maritime and Beta patula. Indicators of efficiency of haploid reduced parthenogenesis in vitro in alloplasmic lines significantly exceeded the best technologies in pollen-sterile lines of sugar beet from 3.79% to 6.25% and had a value of 62.2%, 24.8%, and 16.7%, respectively. Stabilization of genome ploidy to diploid was carried out in selected breeding numbers without colchicine, based on evaluation and selection of genome ploidy using software of ploidy analyzer (AP) Partec. Conclusions. The efficiency of haploid reduced parthenogenesis induction in vitro in apozygotic CMS breeding genotypes of sugar beet as affected by genetic potential of cytoplasm and taking into account the total percentage of haploids (50 units; 100 units) and myxoploids (50 units; 100 units) has been investigated. Homozygous lines were created by stabilizing the genome ploidy of haploid and myxoploid micro sprouts during III–IV passages without the use of colchicine. Technologies of rooting in the open ground for use in the breeding process of sugar beets have been improved.