Under the laboratory conditions, the effect of temperature (10, 15, 20, 25, 30°C) on growth and biochemical composition of Sargassum muticum was studied, the results showed that: the optimum growth temperature of S.muticum was 15 °C in the range of 10-30 °C; the contents of chlorophyll a, carotenoid, soluble protein, soluble sugar and brown algae polyphenols were the highest at the temperature of 25 °C, it was speculated that these components appeared compensatory increase duo to the high temperature stress. The contents of these biochemical components were the lowest at 30 °C.