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Biodentine is a tricalcium silicate cement used as a dentin replacement in restorative dentistry. The aim of this study was to assess the influence of cavity moisture on the immediate sealing and morphology of the interface between Biodentine and the dental tissues. 20 class II cavities were prepared in extracted teeth. 10 cavities were dried using the air spray before restoring with Biodentine and 10 cavities were preserved moist for the restorative procedure. The teeth were subjected to a microleakage test and then the axial sections were observed under optical microscope and scanning electron microscope. In most situations Biodentine provided an immediate good marginal sealing and a tide contact with adjacent tissues in both occlusal and cervical margins which did not seem to be influenced by the moisture of the dental supporting tissues.

The aim of the study is to asses, by using microhardness technique, the remineralization effect of some products used in the treatment of early dental caries adjacent to fixed orthodontic appliances. The study was performed on a group of 32 patients receiving fixed orthodontic therapy. The patients were randomly divided in two groups, accordingly to the administered preventive protocol: in group 1 (control) toothpaste Splat Professional Biocalcium (without fluoride) was applied twice a day; in group 2 (study group) toothpaste and mouthwash Vitis anticaries with natrium monofluorophosphate 1450 ppm F, respectively 226 ppm F, twice a day were used. After 60 days, the extraction of premolars was made and the preparation of the samples was done. The microhardness analysis was performed using the device CV 400 DAT (Namicon). The analysis of the results found lower microhardness for the samples submitted to brushing with fluoridated toothpaste and rinsing with fluoridated mouthwash solution, comparing to control (samples submitted only to brushing with non-fluoridated toothpaste). The conclusions of this study confirm the effectiveness of a proper prophylactic protocol (toothbrushing with fluoridated toothpaste, mouth rinsing with fluoridated mouthwashes) associated to the fixed orthodontics therapy, in the prevention of the early enamel demineralization onset adjacent to brackets.

The aim of the present study was to assess, by profilometry, the effect of different immersion regimes in Coca-Cola drink on the surface roughness of two highly viscous flowable composite resins. The studied materials were Gradia Direct LoFlo (GC Corporation,Tokyo, Japan) and Micro Esthetic flow-viscous (Bisico, Germany). Fifteen cylindrical samples having the of 5 mm and the thickness of 2 mm were made from each material using plastic molds placed on glass plates. The samples were light-cured for 40s on both sides through the glass plates, to ensure complete polymerization of the material. They were divided into two groups as follows: Group I. Gradia Direct LoFlo and Group II. Micro Esthetic flow-viscous. Specimens of each group were then randomly divided into 5 subgroups. In subgroup A (control group) 4 samples were kept only in artificial saliva. In subgroups B-E (each having 4 samples) the samples were immersed in Coca-Cola drink according to 4 different protocols. The mean values of roughness parameters, Ra, were calculated. The Kolmogorov-Smirnov normality test was used to determine the distribution of data in groups. ANOVA and Tukey post hoc statistical tests were used to compare the results from the groups. For both materials, the highest surface roughness was determined for the samples that were submerged 7 times/day in Coca Cola. The assessment of surface microstructure of the samples, after immersion, revealed for both materials a statistically significant increase of Ra values.

The present study was conducted to evaluate the effects of common drinks consumed by patients (Coffee, Coca-Cola, Black Tea) on different types of composite restorations, finishing and surface sealing (nanocomposites, nanohybrids, ormocers) regarding color changes. 45 specimens of composite materials (Nanoceramic, Nanocomposite and Nanohybrid) were made and divided into 3 groups G1-control group, G2 - finished in 2 stages, and G3 glaze coated. The LOVIBOND RT 300 portable spectrophotometer was used to determine the color by following the CIELAB scale. The data was analyzed using Mann-Whitney followed by a multiple comparison t test. Significant differences were found between the groups in terms color changes (p[0.001). The results showed that for both groups of composites (G2 and G3), irrespective of the beverage they were immersed in, the DE values were significantly higher at 7 days compared with the 30 day values.

The aim of study was to evaluate the accuracy of the near-infrared light transillumination technique used in the detection of the proximal incipient dental caries. The study was performed on 12 medium caries risk subjects (mean age of 26.5). A total number of 312 proximal dental surfaces from the posterior dental group were included in the study. All the proximal surfaces, which had not been restored, or unaffected by cavitated dental caries, were assessed by a near-infrared transillumination camera system using light with a 780nm wavelength (DIAGNOcam, KaVO). Bitewing radiographies were performed for all patients included in study. Visual and tactile examination after tooth separation was performed as gold standard to detect the incipient proximal dental caries (ICDAS scores 02 and 03). Statistical analysis using Chi-square test was performed to compare the diagnostic performance of near-infrared light transillumination and the bitewing radiography. Near-infrared light transillumination showed higher sensitivity (81.80%) when compared to bitewing radiography (65.90%) to detect the non-cavitated proximal enamel caries (ICDAS score 02). Near-infrared light transillumination method showed higher sensitivity (100%) when compared to bitewing radiography (81.50%) to detect the incipient cavitated proximal enamel caries (ICDAS score 03). Specificity values for proximal incipient dental caries (ICDAS codes 02 and 03) were 95.03% for near-infrared light transillumination and 99.18% for bitewing radiography. DIAGNOcam (KaVO) is a reliable tool with high diagnostic accuracy of the incipient dental caries localized on proximal surfaces of the posterior dental group.

The aims of the study were to characterize enamel, dentine and cementum surface aspect after using non-fluoride remineralization toothpastes containing calcium carbonate and calcium glycerophosphate by SEM evaluation, to determine chemical composition of enamel, dentin and cement after remineralization products application by EDX assessment, and to compare the remineralization capacity of the tested products. Ten extracted teeth having no dental caries, erosive or wear lesions were selected for the study. The teeth were split bucco-lingually and mesio-distally in four pieces. A free window of enamel, dentine, and cementum (4X4 mm) in the cervical area of the slices was preserved. In control group the slices were subjected to a artificial caries lesion formation. In study groups EcoBio� (President Company), Bio-natural (Dentissimo, Swiss Biodent), and Arthrodont Cassic (Pierre Fabre Oral Care) toothpastes were applied two times a day, for 3 minutes during caries lesion formation. The surface topography has been analyzed using a scanning electron microscope and the quantitative and qualitative chemical composition has been evaluated using an EDX detector. Interprismatic and intraprismatic mineral loss is clearly visible at higher magnification in enamel samples in control group. Some areas of demineralization are still present, and also small areas of extern deposits are visible on enamel, dentine and cementum samples in the groups were tested products were applied. All the tested toothpastes increased calcium and phosphorous ion concentrations in enamel, dentine and cementum. The highest remineralization effect was recorded in enamel by Dentissiomo, followed by Arthrodent and EcoBio toothpastes. In dentine and cementum the highest remineralization effect was registered by Arthrodent, followed by Dentissiomo and EcoBio toothpastes.

The aim of the study was to compare the marginal adaptation of three different resin-based materials used for direct restoration: hybrid composite resin (Gaenial Posterio, GC Corporation), a compomer (Dyract eXtra, Dentsply Sirona), and a giomer (Beautifil II, Shofu Dental) applied at room temperature or after preheated at preheating 50 and 60oC. Class II cavities were prepared on proximal surfaces of extracted teeth and randomly divided in 3 groups according to the material used for restoration. In each group five cavities were restored using the materials at room temperature (subgroup 1), five cavities were restored using preheated materials at 50şC (subgroup 2), and five cavities were restored using preheated materials at 60şC (subgroup 3). The teeth were stored in distilled water for 14 days and then were immersed in 2% methylene blue buffered dye solution (pH = 7) for 4 hours. The teeth were transversally cut and the sections were examined using optical microscope (Carl-Zeiss AXIO Imager A1m) at 50x magnification and the dye penetration was evaluated according to 4 scores. Decreased microleakage and fewer gap formation were recorded for all filling materials after they have been heated at 50 or 60oC. Pre-warming of resin-based materials improves the adaptation of these materials to tooth structures.

The study aimed to assess the changes of the surface roughness induced by some acidic beverages on indirect restorative materials by using profilometry. Twenty samples of three composite resins (Ceramage, SR Adoro, Luna-Wing) and three ceramics (IPS In Line, Hera Ceram, Reflex Dimension) immersed in three acidic beverages (Red Bul, wine, Coca Cola), for 5 minutes, three times daily, 14 days. The control samples were immersed in artificial saliva during the study periode. After the end of the erosive cycles and before to determine the surface roughness of the samples in the study groups, all the samples were immersed in artificial saliva for 18 hours. The surface roughness was determined in relation to the baseline surface using profilometer Perthometer_M1 (Mahr Gottingen GmbH, Germany). The immersion of the indirect restorative materials tested in the three acidic beverages lead has resulted to changes in their surface roughness. The most aggressive was wine, followed by Coca Cola, and Red Bull.

Atherosclerosis is a major component of the cardiovascular diseases and is centered by inflammation but its well-known predictors do not explain some of the atherosclerotic vascular disease events, generating the need to look for independent additional risk factors. Periodontitis, a chronic infection produced by oral bacteria and affecting the supporting structures of the teeth, seems to be linked with atherosclerosis and cardiovascular disease through several mechanisms, like genetic susceptibility, systemic inflammation, infection, and the molecular mimicry, the association being worsened in the presence of diabetes. The epidemiological studies revealed a modest but significant association between periodontal infections and cardiovascular disease, independent of the effect of confounding factors, but the definite effect of periodontitis and its treatment on the incidence of cardiovascular events requires further clarifications.

The aim of this study was to evaluate the influence of different finishing and polishing systems and of composite resins structure on surface state of the restoration. Cylindrical samples from two different composite resins (Filtek Z 550 and Filtek Z 250, 3M ESPE) were obtained. In group 1 the samples were not finished and polished. In groups 2-4 the samples were submitted to finishing procedure using two tungsten carbide burs, then polished using three different polishing systems: three steps diamond abrasive polishers (Kenda CGI, Lichtenstein) - group 2, one step silicon carbide abrasive particles brush (Occlubrush, KerrHawe SA, Switzerland) - group 3 and two steps Sof-Lex spiral wheels (3M ESPE) - group 4. Half of the samples in each group were subjected to surface microstructure evaluation using a scanning electron mycroscope. The other half of the samples was subjected to surface state assessment using profilometery. All the investigated finishing and polishing systems leaded to increased surface roughness when compared to the roughness of composite resins placed in direct contact to celluloid clear matrix. The surface roughness of both microfilled-hybrid and nanofilled-hybrid composite resins finished and polished with different systems were influenced mostly by the polishing system and less by the composite resin structure.

The aim of the study was to evaluate and to compare the compressive parameters of repaired composite restoration when using different types of composite resins and a universal bonding agent as an intermediate layer. Aged micro-filled hybrid and nano-filled hybrid composite resins were chosen to simulate old restoration. The same micro-filled hybrid composite resin was used as a repair material. A universal bonding agent applied in etch-and-rinse and self etch strategies was used as an intermediate layer in restoration repair. Non-aged composite resins were considered as control. Compressive strength and compressive modulus were determined by evaluating the samples in a universal testing machine. Lower values of the tested parameters were recorded after aging both types of composite resin when compared to control. Higher values of compressive strength were recorded when nano-filled hybrid composite resin was repaired when compared to micro-filled hybrid composite resin. The strategy of universal bonding agent application as an intermediate layer did not influenced the compressive properties of repaired restoration.

The aim of this in vitro study was to evaluate the effect of three desensitizing tooth pastes on the dentinal tubule occlusion. Thirty dentin discs having a thickness of 3 mm were obtained by cutting human teeth. The discs were submersed in citric acid for 30 seconds to open the dentin tubules. Then the discs were cut in two halves. In each group 10 halves were kept in 0.9% NaCl solution) and the other 10 halves were exposed to the action of one of the tested desensitizing toothpastes. The dentin samples were placed in the machine designed to simulate tooth brushing. Three commercial desensitizing toothpastes were chosen to be applied on dentin surface. The morphology of dentin samples and the level of tubule occlusion was scored using scanning electron microscope. All the three desensitizing toothpastes demonstrated significant effects on dentinal tubule occlusion. The tooth paste containing arginine and calcium carbonate as active ingredients showed the highest degree of tubule occlusion, followed by the dentifrice containing strontium acetate and sodium fluoride.

The aim of this study was to evaluate and to compare the compression behavior under identical mechanical tests, of three different composite resins, by determining Young�s modulus for compression, ultimate compressive strength and ultimate compressive strain. The studied materials were: Filtek Z250 Universal Restorative, Filtek Z550 and Filtek Bulk Fill Posterior Restorative (3M ESPE, St. Paul, MN, USA). Fifteen cylindrical samples, having 6 mm in height and 5 mm in diameter, were made from each material, using plastic molds. The samples were subjected to quantitative analysis of the compression behavior after mechanical tests. The fractured fragments of the samples were subjected to qualitative surface evaluation by scanning electron microscopy. Results were statistically analyzed using one-way analysis of variance (ANOVA) with Tukey�s post hoc test. Filtek Z250 had the lowest value of Young�s modulus for compression and the results were statistically significant (p[0.05) when compared to Filtek Bulk Fill Posterior Restorative and Filtek Z550. There were no statistically significant differences between all three materials regarding ultimate compressive strength (p]0.05). The lowest value for ultimate compressive strain was recorded for Filtek Bulk Fill.

The aim of this in vitro study was to to evaluate the impact of finishing procedures on the enamel adjacent to composite restorations and to assess if the resistance of the enamel-resin junction to leakage is affected by the use of these instruments. The surfaces of enamel at the joint with composite were observed by scanning electron microscopy, then the microleakages at the enamel margin was assessed using an optical microscope. Finishing with extra-/ ultra-fine carbide burs and extra-fine diamond burs produced a superficial abrasion to the adjacent enamel and did not seem to have a significant influence on the sealing ability of composite resin.

The aims of the study were to characterise the resin-resin interface when a universal bonding agent (UBA) was used in two different strategies in direct composite repair and to evaluate the bonding capacity of UBA by microleakage assessment. In study groups a micro-filled hybrid and a nano-filled hybrid composite resins were aged in order to simulate an old restorations. As a repair material was chosen the same micro-filled hybrid composite resin that was used as an old restoration. UBA was applied in etch-and-rinse and self-etch strategies and was used as an intermediate layer in repair procedure. After the repair the samples were aged again. In control groups were included non-aged, repaired composite resins samples. The resin-resin interface was characterised on SEM images and the microleakage at the interface was evaluated by dye penetration assessment. Universal bonding agent used in direct composite resins repair showed a very good adaptation to non-aged micro-filled hybrid and nano-filled hybrid composite resins. Aging by saliva storage of repaired composite resins leaded to an enlargement of resin-resin junction and a increased microleakage irrespective of the strategy (etch-and-rinse or self -etch) used for bonding agent application. Etch-and-rinse strategy for universal bonging agent application determined a better interface bonding when compared to self -etch strategy.

The aim of this study was to evaluate the postoperative effect of one bleaching agent containing 40% peroxide hydrogen on the interface between dental tissues and a microhybrid composite resin bonded with a universal adhesive applied in total-etch and self-etch technique. Standardized Class V restorations were performed in oral surfaces of 40 extracted human molars with the gingival margins placed bellow the cement-enamel junction. For 20 restorations the adhesive was applied with preliminary etching and for the others the adhesive was applied by using the self-etch technique. Half of the samples of each group were bleached using Opalescence Boost in one session 20 minutes-application. The sealing quality of both enamel and cervical margins of the restorations was evaluated using a microleakage test by immersion in 1% methylene blue for 24 hours. The microleakage scores were assessed using an optical microscope and the data were statistically analyzed. No statistical significant differences were recorded between the bleached and control groups and between the study groups according to the etching strategy used for bonding.

The aim of the study was to investigate the effect of xylitol, from some oral hygiene products and chewing gums, on salivary parameters variation in patients with carious disease. The study was performed on 30 patients with a medium level of carious disease, randomly divided in two groups as follows: group 1 (study group), using xylitol-based products for 30 days, and group 2 (control group), using products without xylitol. The way of administration, dose, and frequency of use were as follows: toothpaste (normal daily brushing, 3 times daily); mouthwashing (5 ml, twice daily); chewing gums (two tablets, 5 times daily), for 30 days. The assessment targeted the salivary parameters as follow: saliva microcrystallisation index (IMK), resting salivary flow rate (RSF), stimulated salivary flow rate(SSF). The results of our study confirm the improvement of salivary parameters due to the use of xylitol-based products, by the positive influence on the remineralisation capacity of saliva. The assessment method of saliva IMK represents a noninvasive, simple, informational indicator for the assessment of the remineralisation capacity of saliva.

The aim of the study was to evaluate the effect of biomimetic self assembling peptides (P11 -4) on remineralization of acute and chronic incipient enamel lesions by microhardness analysis. Ten extracted permanent human teeth having incipient white spot lesions and ten extracted teeth with brown spot lesions on proximal surfaces were included in this study. Half of the extern surface of the lesions was covered with flowable composite resin (Vertise flow, Kerr Corporation) and served as negative control site. On the other half Curodont� Protect gel (Credentis AG, Windisch, Switzerland) was applied 2 times a week, 8 weeks (study). The teeth were cut mesio-distally using diamond disks under cooling water, perpendicularly to the extern surface of the lesions, in order that both areas (negative control and study) to be evaluated. The opposite proximal sound enamel on each section served as positive control. The subsurface microhardness of the enamel samples was determined using digital microhardness tester (MicroVickers Hardness System CV- 400DMTM, CV Instruments Namicon). On horizontal tooth sections indentations were made at 25�m (A1), 75 �m (A2), 125 �m (A3), 175 �m (A4), 225 �m (A5), and 275 �m (A6) bellow the extern surface of the lesions. For white spot and brown spot caries lesions, in both negative control and study groups, the VHN values increased from A1 to A6 areas. In study group VHN values were higher than in negative control group irrespective of the area of examination. The lowest VHN value was registered in negative control A1 area and the highest in study group A6 area. In all six areas of investigation, VHN values in negative control and study groups were lower than the values in positive control group. The use of self-assembling peptides on white spot and brown spot caries lesions increase the hardness of these lesions in the deeper layers of the lesions.