1. Riboflavin-citrate conjugate multicore SPIONs with enhanced magnetic responses and cellular uptake in breast cancer cells
- Author
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Wid Mekseriwattana, Pablo Guardia, Beatriz Torres Herrero, Jesus M. de la Fuente, Chutima Kuhakarn, Anna Roig, Kanlaya Prapainop Katewongsa, Mahidol University, National Research Council of Thailand, Ministry of Higher Education, Science, Research and Innovation (Thailand), Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Center of Excellence for Innovation in Chemistry (Thailand), Diputación General de Aragón, Agencia Estatal de Investigación (España), Mekseriwattana, Wid, Torres Herrero, Beatriz 0000-0003-4648-8972], Kuhakarn, Chutima, Roig Serra, Anna, Katewongsa, Kanlaya Prapainop, Mekseriwattana, Wid [0000-0002-0966-6293], Kuhakarn, Chutima [0000-0003-4638-4356], Roig Serra, Anna [0000-0001-6464-7573], and Katewongsa, Kanlaya Prapainop [0000-0001-6417-4921]
- Subjects
Particle-size ,General Engineering ,General Materials Science ,Bioengineering ,Hyperthermia ,General Chemistry ,Iron-oxide nanoparticles ,Citric-acid ,Atomic and Molecular Physics, and Optics - Abstract
Breast cancer accounts for up to 10% of the newly diagnosed cancer cases worldwide, making it the most common cancer found in women. The use of superparamagnetic iron oxide nanoparticles (SPIONs) has been beneficial in the advancement of contrast agents and magnetic hyperthermia (MH) for the diagnosis and treatment of cancers. To achieve delivery of SPIONs to cancer cells, surface functionalization with specific ligands are required. Riboflavin carrier protein (RCP) has been identified as an alternative target for breast cancer cells. Here, we report a novel riboflavin (Rf)-based ligand that provides SPIONs with enhanced colloidal stability and high uptake potential in breast cancer cells. This is achieved by synthesizing an Rf-citrate ligand. The ligand was tested in a multicore SPION system, and affinity to RCP was assessed by isothermal titration calorimetry which showed a specific, entropy-driven binding. MRI and MH responses of the coated Rf-SPIONs were tested to evaluate the suitability of this system as a theranostic platform. Finally, interaction of the Rf-SPIONs with breast cancer cells was evaluated by in vitro cellular uptake in MCF-7 breast cancer cells. The overall characterization of the Rf-SPIONs highlighted the excellent performance of this platform for theranostic applications in breast cancer., This project was supported by Mahidol University under a New Discovery and Frontier Research Grant to K. P. K. This project is funded by National Research Council of Thailand (NRCT) and Mahidol University: N42A650357. Partial support was also provided by a Central Instrument Facility (CIF) grant and the Center for Nanoimaging, Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research and Innovation, Faculty of Science, Mahidol University. W. M. was supported by the Science Achievement Scholarship of Thailand. The authors would like to thank Associate Professor Nuttawee Niamsiri, Department of Biotechnology, Faculty of Science, Mahidol University, for the use of the Zetasizer instrument. This research was also funded by the Spanish Government, through the “Severo Ochoa” Programme for Centers of Excellence in R&D (FUNFUTURE, CEX2019-000917 S) and DGA, through the “Fondo Social Europeo” (DGA E15_20R). B. T. H. is grateful for a predoctoral fellowship FPU (FPU19/01311). P. G. acknowledges financial support from the Spanish government (MICIU) through the Ramon y Cajal research program (RyC2019-028414-I).
- Published
- 2022