1. [Frequency of genes speA, speB, and speC in Streptococcus pyogenes strains and the identification of the infective agent by polymerase chain reaction].
- Author
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Dmitrieva NF, Trofimov DIu, Eshchina AS, Riapis LA, Pavlova OG, Petrova TV, Skorkina IuA, Gerasimov AN, Alekseev LP, Zhuravlev MV, and Briko NI
- Subjects
- DNA Primers, Exotoxins analysis, Humans, Polymerase Chain Reaction methods, Russia epidemiology, Species Specificity, Streptococcal Infections epidemiology, Streptococcal Infections microbiology, Bacterial Proteins, Exotoxins genetics, Gene Frequency, Genes, Bacterial, Membrane Proteins, Streptococcus pyogenes genetics, Streptococcus pyogenes isolation & purification
- Abstract
In cultures of S. pyogenes isolated from patients and carriers in different territories of the Russian Federation the genes of erythorogenic toxins A, B and C (speA, speB and specC) were detected. The possibility of the identification of S. pyogenes by means of PCR on the basis of primers to erythrogenic toxin B was determined. Gene speB was detected in all S. pyogenes cultures under study and proved to be species specific. Genes speA and speC were detected, respectively, in 29.4% and 9.35% of the S. pyogenes cultures under study. A test system for the identification of S. pyogenes on the basis of primers to gene speB was developed. The prospects for the detection of genes speA and speC for intraspecific typing of this infective agent were evaluated.
- Published
- 2002