1. Detection of trace processes in the networks of neurons cultured on microelectrode arrays.
- Author
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Sukhanova AL, Mineyeva OA, Kiselev II, Burtsev MS, and Anokhin KV
- Subjects
- Animals, Cell Culture Techniques, Electric Stimulation, Fluorescent Antibody Technique, Mice, Mice, Inbred BALB C, Gene Expression Regulation physiology, Hippocampus cytology, Microelectrodes, Neuronal Plasticity physiology, Neurons metabolism, Proto-Oncogene Proteins c-fos metabolism
- Abstract
We compared the effects of electrical stimulation of primary dissociated neuronal cultures cultured on microelectrode arrays in terms on the expression of c-fos transcriptional factor that is involved in plastic reorganization in neurons. Dissociated hippocampal neurons cultured on multielectrode arrays were exposed to two stimulation protocols: high-frequency and low-frequency stimulations. Expression of c-fos was evaluated using immunofluorescence. Both high-frequency and low-frequency stimulations significantly increased c-fos expression in comparison with non-stimulated control. These findings indicate that c-fos expression can be induced in neuronal cell culture by different types of electrical stimulations and can be used for studying plasticity processes in microphysiological in vitro systems.
- Published
- 2012
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