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Your search keyword '"Grabovetskiĭ VV"' showing total 3 results
Start Over Author "Grabovetskiĭ VV" Topic gene expression regulation, bacterial
3 results on '"Grabovetskiĭ VV"'

1. [Expression of Staphylococcus aureus enterotoxin A gene in heterologous systems].

Author

Chikindas ML, Isaeva SV, Chistiakova LG, Klinskiĭ EIu, Grabovetskiĭ VV, Vasilenko OV, Sveshnikov PG, Alakhov VIu, and Kiselev VI

Subjects
Bacillus subtilis genetics, Escherichia coli genetics, Gene Library, Plasmids, Restriction Mapping, Enterotoxins genetics, Gene Expression Regulation, Bacterial, Genes, Bacterial, Staphylococcus aureus genetics
Abstract

The genomic library of Staphylococcus aureus genes on the plasmid vector pSL5 has been constructed. The library contains a 2.5 kb HindIII DNA fragment including the gene for enterotoxin A. The entA gene on the high copy number plasmids in the Escherichia coli cells deficient in proteolysis determines the synthesis of enterotoxin A in the amounts comparable to the ones in the parent strain Staphylococcus aureus FRI 722(H).

Published
1990

2. [The cloning and expression of the Staphylococcus aureus enterotoxin A gene in Escherichia coli cells].

Author

Chikindas ML, Grabovetskiĭ VV, Melik-Nubarov NS, Klinskiĭ EIu, Vasilenko OV, Sveshnikov PG, Alakhov VIu, and Kiselev VI

Subjects
Coliphages genetics, DNA, Bacterial genetics, Gene Library, Genetic Vectors genetics, Plasmids genetics, Staphylococcus Phages genetics, Transfection genetics, Cloning, Molecular methods, Enterotoxins genetics, Escherichia coli genetics, Gene Expression Regulation, Bacterial genetics, Genes, Bacterial, Staphylococcus aureus genetics
Abstract

Gene ent-A has been cloned on phage vector pSL5 with the use of the gene library of S. aureus FR1722(H). It is located within DNA fragment Hind III having 2,500 nucleotide pairs.

Published
1990

3. [Thermo-regulated expression of the htpR gene under the control of the PR-promotor of bacteriophage lambda induces supersynthesis of heat shock proteins].

Author

Kiselev VI, Tarasova IM, Shchepetov ME, and Grabovetskiĭ VV

Subjects
Autoradiography, Escherichia coli metabolism, Plasmids, Promoter Regions, Genetic, Bacteriophage lambda genetics, Escherichia coli genetics, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Viral, Heat-Shock Proteins biosynthesis
Abstract

The mechanisms of induction of heat shock protein synthesis in E. coli have been studied. For this purpose plasmids in which htpR gene expression is controlled by the PR-promoter of bacteriophage lambda and by the Trp-promoter have been constructed. An effective induction of heat shock proteins requires both an increased content of htpR protein and additional cofactors formed in the cell under heat shock conditions.

Published
1989

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