4 results on '"Winn, Virginia D."'
Search Results
2. Molecular networks in atopic mothers impact the risk of infant atopy.
- Author
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Schedel, Michaela, Leach, Sonia M., Strand, Matthew J., Danhorn, Thomas, MacBeth, Morgan, Faino, Anna V., Lynch, Anne M., Winn, Virginia D., Munoz, Lindsay L., Forsberg, Shannon M., Schwartz, David A., Gelfand, Erwin W., and Hauk, Pia J.
- Subjects
ATOPY ,MOTHERS ,GENE expression ,GENOMICS ,PRINCIPAL components analysis - Abstract
Background: The prevalence of atopic diseases has increased with atopic dermatitis (AD) as the earliest manifestation. We assessed if molecular risk factors in atopic mothers influence their infants' susceptibility to an atopic disease. Methods: Pregnant women and their infants with (n = 174, high‐risk) or without (n = 126, low‐risk) parental atopy were enrolled in a prospective birth cohort. Global differentially methylated regions (DMRs) were determined in atopic (n = 92) and non‐atopic (n = 82) mothers. Principal component analysis was used to predict atopy risk in children dependent on maternal atopy. Genome‐wide transcriptomic analyses were performed in paired atopic (n = 20) and non‐atopic (n = 15) mothers and cord blood. Integrative genomic analyses were conducted to define methylation–gene expression relationships. Results: Atopic dermatitis was more prevalent in high‐risk compared to low‐risk children by age 2. Differential methylation analyses identified 165 DMRs distinguishing atopic from non‐atopic mothers. Inclusion of DMRs in addition to maternal atopy significantly increased the odds ratio to develop AD in children from 2.56 to 4.26. In atopic compared to non‐atopic mothers, 139 differentially expressed genes (DEGs) were identified significantly enriched of genes within the interferon signaling pathway. Expression quantitative trait methylation analyses dependent on maternal atopy identified 29 DEGs controlled by 136 trans‐acting methylation marks, some located near transcription factors. Differential expression for the same nine genes, including MX1 and IFI6 within the interferon pathway, was identified in atopic and non‐atopic mothers and high‐risk and low‐risk children. Conclusion: These data suggest that in utero epigenetic and transcriptomic mechanisms predominantly involving the interferon pathway may impact and predict the development of infant atopy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. Murine trophoblast-derived and pregnancy-associated exosome-enriched extracellular vesicle microRNAs: Implications for placenta driven effects on maternal physiology.
- Author
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Stefanski, Adrianne L., Martinez, Nadine, Peterson, Lisa K., Callahan, Tiffany J., Treacy, Eric, Luck, Marisa, Friend, Samantha F., Hermesch, Amy, Maltepe, Emin, Phang, Tzu, Dragone, Leonard L., and Winn, Virginia D.
- Subjects
EXOSOMES ,TROPHOBLAST ,MICRORNA ,CELL communication ,X chromosome - Abstract
The role of extracellular vesicles (EVs), specifically exosomes, in intercellular communication likely plays a key role in placental orchestration of pregnancy and maternal immune sensing of the fetus. While murine models are powerful tools to study pregnancy and maternal-fetal immune interactions, in contrast to human placental exosomes, the content of murine placental and pregnancy exosomes remains largely understudied. Using a recently developed in vitro culture technique, murine trophoblast stem cells derived from B6 mice were differentiated into syncytial-like cells. EVs from the conditioned media, as well as from pregnant and non-pregnant sera, were enriched for exosomes. The RNA composition of these murine trophoblast-derived and pregnancy-associated exosome-enriched-EVs (ExoE-EVs) was determined using RNA-sequencing analysis and expression levels confirmed by qRT-PCR. Differentially abundant miRNAs were detected in syncytial differentiated ExoE-EVs, particularly from the X chromosome cluster (mmu-miR-322-3p, mmu-miR-322-5p, mmu-miR-503-5p, mmu-miR-542-3p, and mmu-miR-450a-5p). These were confirmed to be increased in pregnant mouse sera ExoE-EVs by qRT-PCR analysis. Interestingly, fifteen miRNAs were only present within the pregnancy-derived ExoE-EVs compared to non-pregnant controls. Mmu-miR-292-3p and mmu-miR-183-5p were noted to be some of the most abundant miRNAs in syncytial ExoE-EVs and were also present at higher levels in pregnant versus non-pregnant sera ExoE-EVs. The bioinformatics tool, MultiMir, was employed to query publicly available databases of predicted miRNA-target interactions. This analysis reveals that the X-chromosome miRNAs are predicted to target ubiquitin-mediated proteolysis and intracellular signaling pathways. Knowing the cargo of placental and pregnancy-specific ExoE-EVs as well as the predicted biological targets informs studies using murine models to examine not only maternal-fetal immune interactions but also the physiologic consequences of placental-maternal communication. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
4. The impact of preeclampsia on gene expression at the maternal–fetal interface.
- Author
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Winn, Virginia D., Gormley, Matthew, and Fisher, Susan J.
- Subjects
PREECLAMPSIA ,PREGNANCY ,HYPERTENSION ,PROTEINURIA ,GENE expression - Abstract
Abstract: Preeclampsia (PE) impacts 8 million mother–infant pairs worldwide each year. This human pregnancy-specific disease characterized by hypertension and proteinuria accounts for significant maternal and neonatal morbidity and mortality. The current theory of the pathogenesis of PE as reviewed by Dr. Christopher Redman and Dr. Ian Sargent is thought to occur as a 2-stage process with poor placentation in the first half of pregnancy resulting in the maternal response in the second half of pregnancy. Our studies have focused on understanding the placental contribution to this serious disease by examining the gene expression profile of the deciduas basalis or basal plate, the region of the placenta involved in the “poor placentation”. In this review we present summaries of our microarray datasets both of normal placentation and those of gene expression changes resulting in the context of PE. Additionally, we have taken this opportunity to combine the datasets to provide a more comprehensive view of this region of the placenta. As defects in the basal plate are, in part, at the root of the disease process, we believe that understanding the pathobiology that occurs in this region will increase our ability to alter the development and/or course of PE. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
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