1. A minimal domain responsible for Munc13 activity.
- Author
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Basu J, Shen N, Dulubova I, Lu J, Guan R, Guryev O, Grishin NV, Rosenmund C, and Rizo J
- Subjects
- Animals, Chromatography, Gel, Computational Biology, Excitatory Postsynaptic Potentials, Exocytosis genetics, Mice, Mice, Knockout, Nerve Tissue Proteins metabolism, Neurons drug effects, Neurotransmitter Agents metabolism, Nuclear Magnetic Resonance, Biomolecular, Protein Folding, Protein Structure, Secondary genetics, Protein Structure, Tertiary, Rats, Structure-Activity Relationship, Sucrose pharmacology, Exocytosis physiology, Gene Expression, Hippocampus metabolism, Nerve Tissue Proteins genetics, Neurons metabolism
- Abstract
Munc13 proteins are essential in neurotransmitter release, controlling the priming of synaptic vesicles to a release-ready state. The sequences responsible for this priming activity are unknown. Here we identify a large alpha-helical domain of mammalian Munc13-1 that is autonomously folded and is sufficient to rescue the total arrest in neurotransmitter release observed in hippocampal neurons lacking Munc13s.
- Published
- 2005
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