8 results on '"Roitsch, Thomas"'
Search Results
2. Physiological and molecular analysis of the interaction between aluminium toxicity and drought stress in common bean ( Phaseolus vulgaris )
- Author
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Yang, Zhong-Bao, Eticha, Dejene, Albacete, Alfonso, Rao, Idupulapati Madhusudana, Roitsch, Thomas, and Horst, Walter Johannes
- Published
- 2012
3. Metabolic Consequences of Infection of Grapevine (Vitis vinifera L.) cv. "Modra frankinja" with Flavescence Dorée Phytoplasma.
- Author
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Prezelj, Nina, Covington, Elizabeth, Roitsch, Thomas, Gruden, Kristina, Fragner, Lena, Weckwerth, Wolfram, Chersicola, Marko, Vodopivec, Maja, Dermastia, Marina, Zhilong Bao, and Allwood, Will
- Subjects
GRAPE diseases & pests ,PHYTOPLASMA diseases ,PLANT metabolism - Abstract
Flavescence dorée, caused by the quarantine phytoplasma FDp, represents the most devastating of the grapevine yellows diseases in Europe. In an integrated study we have explored the FDp-grapevine interaction in infected grapevines of cv. "Modra frankinja" under natural conditions in the vineyard. In FDp-infected leaf vein-enriched tissues, the seasonal transcriptional profiles of 14 genes selected from various metabolic pathways showed an FDp-specific plant response compared to other grapevine yellows and uncovered a new association of the SWEET17a vacuolar transporter of fructose with pathogens. Non-targeted metabolome analysis from leaf vein-enriched tissues identified 22 significantly changed compounds with increased levels during infection. Several metabolites corroborated the gene expression study. Detailed investigation of the dynamics of carbohydrate metabolism revealed significant accumulation of sucrose and starch in the mesophyll of FDp-infected leaves, as well as significant up-regulation of genes involved in their biosynthesis. In addition, infected leaves had high activities of ADP-glucose pyrophosphorylase and, more significantly, sucrose synthase. The data support the conclusion that FDp infection inhibits phloem transport, resulting in accumulation of carbohydrates and secondary metabolites that provoke a source-sink transition and defense response status. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
4. Homo- and heterodimers of tobacco bZIP proteins counteract as positive or negative regulators of transcription during pollen development.
- Author
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Tim Iven, Strathmann, Anne, Böttner, Stefan, Zwafink, Thomas, Heinekamp, Thorsten, Guivarc'h, Anne, Roitsch, Thomas, and Dröge-Laser, Wolfgang
- Subjects
GENE expression ,LEUCINE ,GENETIC transcription ,INVERTASE ,TAPETUM - Abstract
Expression of BZI-1ΔN, a dominant-negative form of the tobacco ( Nicotiana tabacum) basic leucine zipper (bZIP) transcription factor BZI-1 leads to severe defects in pollen development which coincides with reduced transcript abundance of the stamen specific invertase gene NIN88 and decreased extracellular invertase enzymatic activity. This finding suggests a function of BZI-1 in regulating carbohydrate supply of the developing pollen. BZI-1 heterodimerises with the bZIP factors BZI-2, BZI-3 and BZI-4 in vitro and in planta. Whereas BZI-1 exhibits only weak activation properties, BZI-1/BZI-2 heterodimers strongly activate transcription. Consistently, approaches leading to reduced levels of functional BZI-1 or BZI-2 both significantly interfere with pollen development, auxin responsiveness and carbohydrate partitioning. In situ hybridisation studies for BZI-1 and BZI-2 confirmed temporal and spatial overlapping expression patterns in tapetum and pollen supporting functional cooperation of these factors during pollen development. Plants over-expressing BZI-4 produce significantly reduced amounts of intact pollen and are also impaired in NIN88 transcription and enzymatic activity. BZI-4 homodimer efficiently binds to a G-box located in the NIN88 promoter but exhibits almost no transcriptional activation capacity. As BZI-4 does not actively repress transcription, we propose that its homodimer blocks G-box mediated transcription. In summary, these data support a regulatory model in which BZI-4 homodimers and BZI-1/BZI-2 heterodimers perform opposing functions as negative or positive transcriptional regulators during pollen development. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
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5. Arbuscular mycorrhiza induces gene expression of the apoplastic invertase LIN6 in tomato (Lycopersicon esculentum) roots.
- Author
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Schaarschmidt, Sara, Roitsch, Thomas, and Hause, Bettina
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VESICULAR-arbuscular mycorrhizas , *GENE expression , *PLANT metabolism , *GENETIC regulation , *BIOCHEMISTRY - Abstract
Extracellular invertases are suggested to play a crucial role in the arbuscular mycorrhiza (AM) symbiosis to fulfil the increased sink function of the mycorrhizal root and the supply of the obligate biotrophic AM fungus with hexoses. In tomato (Lycopersicon esculentum), LIN6 represents an apoplastic invertase which is described as a key enzyme in establishing and maintaining sink metabolism. In this study, transcript levels of LIN6 were analysed in tomato roots colonized with the AM fungus Glomus intraradices. Using real-time RT–PCR, a nearly 3-fold increase in LIN6 mRNA levels was detected at late stages of mycorrhization (11 weeks after inoculation). A 1.8-fold induction could already be achieved at earlier stages (5 weeks after inoculation) using higher inoculum concentrations, whereas wounding of non-mycorrhizal roots resulted in up to 12-fold enhanced LIN6 transcripts. As revealed by in situ hybridization, the expression of LIN6 upon mycorrhization was specifically restricted to colonized cells and to the central cylinder. Such a strongly localized pattern due to mycorrhizal cells and to the central core could also be shown for promoter activity using transgenic Nicotiana tabacum plants expressing the gene coding for β-glucuronidase under the control of the LIN6 promoter. The moderate induction of LIN6 expression in mycorrhizal tomato roots compared with stress-stimulated induction suggested a fine-tuning in the activation of sink metabolism in the mutualistic interaction, avoiding stress-induced defence reactions. [ABSTRACT FROM PUBLISHER]
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- 2006
- Full Text
- View/download PDF
6. Complex regulation of gene expression, photosynthesis and sugar levels by pathogen infection in tomato.
- Author
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Berger, Susanne, Papadopoulos, Martina, Schreiber, Ulrich, Kaiser, Werner, and Roitsch, Thomas
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TOMATO diseases & pests ,TOMATO disease & pest resistance ,GENE expression ,PHOTOSYNTHESIS ,PATHOGENIC microorganisms ,SUGAR - Abstract
The infection of plants with pathogens results in the induction of defence reactions as well as changes in carbohydrate metabolism. On the one hand, the pathogen attempts to manipulate the carbohydrate metabolism of the plant for its own advantage. On the other, the plant has to reorganize carbon fluxes to ensure fight against the pathogen. in order to further investigate the connection between pathogen infection and carbohydrate metabolism, the effects of two types of pathogen, biotrophic and necrotrophic, on gene expression, endogenous sugar levels and photosynthesis of tomato plants were analysed. Photosynthetic gene expression was downregulated on infection with Pseudomonas syringae and Botrytis cinerea. in contrast, expression of a sink-specific gene encoding a cell wall invertase and of defence genes was induced by both pathogens. These results provide evidence for a co-regulation of defence, sink and photosynthetic gene expression in planta in response to both types of pathogen. The brassinosteroid-containing plant restorative ComCat enhanced resistance against B. cinerea and counter-regulated the repression of photosynthetic gene expression. Endogenous sugar levels decreased and the hexose to sucrose ratio increased on treatment with B. cinerea. The application of chlorophyll fluorescence imaging revealed the spatio-ternporal heterogeneity of the pathogen response. At 24 h after infection, inhibition of photosynthetic electron transport was restricted to the direct vicinity of the infection site, which was surrounded by a circle of increased photosynthetic activity. The photosynthesis of the remaining leaf was not affected at this stage. These results show the usefulness of chlorophyll fluorescence imaging for the assessment of the complex spatio-temporal changes and for the definition of the areas relevant for other types of determination, e.g. gene expression. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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7. Tomato growth promotion by the fungal endophytes Serendipita indica and Serendipita herbamans is associated with sucrose de-novo synthesis in roots and differential local and systemic effects on carbohydrate metabolisms and gene expression.
- Author
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De Rocchis, Vincenzo, Jammer, Alexandra, Camehl, Iris, Franken, Philipp, and Roitsch, Thomas
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CARBOHYDRATE metabolism , *ENDOPHYTIC fungi , *SUCROSE , *PLANT growth , *GENE expression , *PLANT metabolism , *GLYCOLYSIS , *PHYTOCHELATINS - Abstract
Plant growth-promoting and stress resilience-inducing root endophytic fungi represent an additional carbohydrate sink. This study aims to test if such root endophytes affect the sugar metabolism of the host plant to divert the flow of resources for their purposes. Fresh and dry weights of roots and shoots of tomato (Solanum lycopersicum) colonised by the closely related Serendipita indica and Serendipita herbamans were recorded. Plant carbohydrate metabolism was analysed by measuring sugar levels, by determining activity signatures of key enzymes of carbohydrate metabolism, and by quantifying mRNA levels of genes involved in sugar transport and turnover. During the interaction with the tomato plants, both fungi promoted root growth and shifted shoot biomass from stem to leaf tissues, resulting in increased leaf size. A common effect induced by both fungi was the inhibition of phosphofructokinase (PFK) in roots and leaves. This glycolytic-pacing enzyme shows how the glycolysis rate is reduced in plants and, eventually, how sugars are allocated to different tissues. Sucrose phosphate synthase (SPS) activity was strongly induced in colonised roots. This was accompanied by increased SPS-A1 gene expression in S. herbamans -colonised roots and by increased sucrose amounts in roots colonised by S. indica. Other enzyme activities were barely affected by S. indica , but mainly induced in leaves of S. herbamans -colonised plants and decreased in roots. This study suggests that two closely related root endophytic fungi differentially influence plant carbohydrate metabolism locally and systemically, but both induce a similar increase in plant biomass. Notably, both fungal endophytes induce an increase in SPS activity and, in the case of S. indica, sucrose resynthesis in roots. In leaves of S. indica -colonised plants, SWEET11b expression was enhanced, thus we assume that excess sucrose was exported by this transporter to the roots. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
8. Cloning and characterization of a novel LpWRKY1 transcription factor in tomato
- Author
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Hofmann, Markus Georg, Sinha, Alok Krishna, Proels, Reinhard Korbinian, and Roitsch, Thomas
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CLONING , *GENE expression , *TOMATOES , *MESSENGER RNA - Abstract
Abstract: The initiation of defence responses in plants is accompanied by fundamental changes in gene expression: the expression of pathogenesis-related genes is co-ordinately regulated with metabolic changes such as down regulation of photosynthesis and induction of sink metabolism. To identify candidate regulators of this co-ordinated regulatory mechanism, the role of WRKY transcription factors in the initiation of defence response was analysed in tomato. A WRKY-type transcription factor (LpWRKY1) from tomato was cloned by a reverse Northern approach. The corresponding mRNA is rapidly and transiently induced after challenging the cells with an elicitor-preparation derived from the wilt inducing fungus Fusarium oxysporum lycopersici (E-FOL) and the fungal elicitor chitosan, whereas the endogenous signals systemin and salicylic acid are inactive. Inhibition of protein biosynthesis by cycloheximide results in sustained induction of mRNA for LpWRKY1. In contrast, the transient induction of the gene encoding LpWRKY1 in response to elicitation by E-FOL is inhibited by the protein-kinase inhibitor staurosporine and may be mimicked by the phosphatase inhibitors endothall and cantharidine indicating the involvement of protein phosphorylation in the regulation of WRKY-type transcription factors. Direct proof of this postranslational modification of LpWRKY1 was obtained by demonstrating in-gel kinase assays using recombinant LpWRKY1 as substrate. A 44kDa and a 67kDa protein kinase were shown to be transiently activated to phosphorylate LpWRKY1 protein in response to elicitation with E-FOL. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
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