1. Feed restriction significantly alters lipogenic gene expression in broiler breeder chickens.
- Author
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Richards MP, Poch SM, Coon CN, Rosebrough RW, Ashwell CM, and McMurtry JP
- Subjects
- ATP Citrate (pro-S)-Lyase genetics, Abdomen, Acetyl-CoA Carboxylase genetics, Adipose Tissue chemistry, Animals, Apolipoprotein A-I genetics, Apolipoproteins genetics, Apolipoproteins B genetics, CCAAT-Enhancer-Binding Proteins genetics, Carrier Proteins genetics, DNA-Binding Proteins genetics, Estradiol blood, Fatty Acid Synthases genetics, Fatty Acid-Binding Proteins, Light, Lipoproteins, VLDL genetics, Liver chemistry, Malate Dehydrogenase genetics, Oviposition, RNA, Messenger analysis, Stearoyl-CoA Desaturase genetics, Sterol Regulatory Element Binding Protein 1, Chickens metabolism, Food Deprivation, Gene Expression, Lipids biosynthesis, Lipids genetics, Neoplasm Proteins, Transcription Factors
- Abstract
Broiler breeder pullets were divided into two groups at 21 wk of age. One group was given free access to feed (ad libitum) and the other fed a limited amount of feed (restricted). At 22 wk, all birds were photostimulated and maintained throughout an egg-laying cycle ending at 36 wk. Samples of liver and abdominal fat pad were collected just before photostimulation (prelight), after photostimulation at first egg and at peak egg production (plateau). Hepatic expression of sterol regulatory element binding protein-1, ATP-citrate lyase, fatty acid synthase, malic enzyme, acetyl-CoA carboxylase and stearoyl-CoA (Delta9) desaturase 1 genes in ad libitum birds declined from their highest levels just before photostimulation as the birds came into and maintained egg production. In contrast, the restricted birds had significant (P < 0.05) increases in the expression of these genes after photostimulation at first egg with a subsequent decline as they reached peak egg production. Hepatic expression of fatty acid binding protein, VLDL apolipoprotein (apoVLDL-II) and apoB genes increased significantly (P < 0.05) in both ad libitum and restricted breeders after photostimulation, whereas apoA1 gene expression declined during this time. Abdominal fat pad weights were significantly (P < 0.05) higher in the ad libitum compared with restricted birds after photostimulation. Lipoprotein lipase in this tissue showed a pattern of expression similar to that observed for the hepatic lipogenic enzyme genes. In conclusion, feed restriction during the pullet-to-breeder transition period significantly (P < 0.05) altered hepatic lipogenic gene expression in broiler breeders.
- Published
- 2003
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