1. Effect of a genetic polymorphism in SREBP1 on fatty acid composition and related gene expression in subcutaneous fat tissue of beef cattle breeds.
- Author
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Gamarra D, Aldai N, Arakawa A, de Pancorbo MM, and Taniguchi M
- Subjects
- Alleles, Animals, Back, Female, Genotype, Introns genetics, Nutritive Value, Phylogeny, RNA, Messenger genetics, RNA, Messenger metabolism, Red Meat, Cattle genetics, Cattle metabolism, Fatty Acids metabolism, Gene Expression, Polymorphism, Genetic genetics, Sterol Regulatory Element Binding Protein 1 genetics, Sterol Regulatory Element Binding Protein 1 metabolism, Subcutaneous Fat metabolism
- Abstract
Sterol regulatory element-binding factor 1 (SREBP1) plays an important role in the lipogenesis which affects fatty acid (FA) composition in backfat and consequently influences beef nutritional quality. This study analyzed the association of 84 bp-indel, both short (S) and long (L) alleles in intron 5 of SREBP1, with FA composition and gene expression of SREBP1 in backfat of northern Spanish beef breeds (Pirenaica, Salers and Holstein-Friesian). Phylogenetic analysis suggests that 84 bp-indel of ruminants is a highly conserved region compared with those in the full-length sequence of intron 5 or mRNA of SREBP1 among species. Overall, higher content of polyunsaturated FAs was observed in SL genotype compared to LL genotype of 84 bp-Indel (p < .05). In particular, in Pirenaica, SL genotype was associated with a higher content of stearic (18:0), α-linolenic (18:3n-3) acid, and total n-3 content (p < .05). However, the gene expression of SREBP1 did not differ among genotypes of 84 bp-Indel (p > .05)., (© 2021 Japanese Society of Animal Science.)
- Published
- 2021
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