6 results on '"Gulisano, Massimo"'
Search Results
2. Novel Mechanisms of Spinal Cord Plasticity in a Mouse Model of Motoneuron Disease.
- Author
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Gulino, Rosario, Parenti, Rosalba, and Gulisano, Massimo
- Subjects
ANIMAL experimentation ,BIOLOGICAL models ,DNA ,GENE expression ,MICE ,MOTOR neuron diseases ,MULTIVARIATE analysis ,NEUROPLASTICITY ,PROTEINS ,REGRESSION analysis ,RNA ,SPINAL cord ,STATISTICS ,DATA analysis ,ONE-way analysis of variance - Abstract
A hopeful spinal cord repairing strategy involves the activation of neural precursor cells. Unfortunately, their ability to generate neurons after injury appears limited. Another process promoting functional recovery is synaptic plasticity. We have previously studied some mechanisms of spinal plasticity involving BDNF, Shh, Notch-1, Numb, and Noggin, by using a mouse model of motoneuron depletion induced by cholera toxin-B saporin. TDP-43 is a nuclear RNA/DNA binding protein involved in amyotrophic lateral sclerosis. Interestingly, TDP-43 could be localized at the synapse and affect synaptic strength. Here, we would like to deepen the investigation of this model of spinal plasticity. After lesion, we observed a glial reaction and an activity-dependent modification of Shh, Noggin, and Numb proteins. By using multivariate regression models, we found that Shh and Noggin could affect motor performance and that these proteins could be associated with both TDP-43 and Numb. Our data suggest that TDP-43 is likely an important regulator of synaptic plasticity, probably in collaboration with other proteins involved in both neurogenesis and synaptic plasticity. Moreover, given the rapidly increasing knowledge about spinal cord plasticity, we believe that further efforts to achieve spinal cord repair by stimulating the intrinsic potential of spinal cord will produce interesting results. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
3. Gene Expression Analysis of PTEN Positive Glioblastoma Stem Cells Identifies DUB3 and Wee1 Modulation in a Cell Differentiation Model.
- Author
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Forte, Stefano, Pagliuca, Alfredo, Maniscalchi, Eugenia T., Gulino, Rosario, Calabrese, Giovanna, Ricci-Vitiani, Lucia, Pallini, Roberto, Signore, Michele, Parenti, Rosalba, De Maria, Ruggero, and Gulisano, Massimo
- Subjects
GLIOBLASTOMA multiforme ,GENE expression ,ASTROCYTOMAS ,BRAIN tumors ,CANCER stem cells ,CANCER cell differentiation ,TUMOR suppressor proteins ,CANCER cell culture ,PROGNOSIS - Abstract
The term astrocytoma defines a quite heterogeneous group of neoplastic diseases that collectively represent the most frequent brain tumors in humans. Among them, glioblastoma multiforme represents the most malignant form and its associated prognosis is one of the poorest among tumors of the central nervous system. It has been demonstrated that a small population of tumor cells, isolated from the brain neoplastic tissue, can reproduce the parental tumor when transplanted in immunodeficient mouse. These tumor initiating cells are supposed to be involved in cancer development and progression and possess stem cell-like features; like their normal counterpart, these cells remain quiescent until they are committed to differentiation. Many studies have shown that the role of the tumor suppressor protein PTEN in cell cycle progression is fundamental for tumor dynamics: in low grade gliomas, PTEN contributes to maintain cells in G1 while the loss of its activity is frequently observed in high grade gliomas. The mechanisms underlying the above described PTEN activity have been studied in many tumors, but those involved in the maintenance of tumor initiating cells quiescence remain to be investigated in more detail. The aim of the present study is to shed light on the role of PTEN pathway on cell cycle regulation in Glioblastoma stem cells, through a cell differentiation model. Our results suggest the existence of a molecular mechanism, that involves DUB3 and WEE1 gene products in the regulation of Cdc25a, as functional effector of the PTEN/Akt pathway. [ABSTRACT FROM AUTHOR]
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- 2013
- Full Text
- View/download PDF
4. Proteomic and Carbonylation Profile Analysis of Rat Skeletal Muscles following Acute Swimming Exercise.
- Author
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Magherini, Francesca, Gamberi, Tania, Pietrovito, Laura, Fiaschi, Tania, Bini, Luca, Esposito, Fabio, Marini, Marina, Abruzzo, Provvidenza Maria, Gulisano, Massimo, and Modesti, Alessandra
- Subjects
PROTEOMICS ,CARBONYLATION ,SKELETAL muscle ,SWIMMING ,GENE expression ,MUSCULOSKELETAL system ,CELLULAR signal transduction ,LABORATORY rats - Abstract
Previous studies by us and other groups characterized protein expression variation following long-term moderate training, whereas the effects of single bursts of exercise are less known. Making use of a proteomic approach, we investigated the effects of acute swimming exercise (ASE) on protein expression and carbonylation patterns in two hind limb muscles: the Extensor Digitorum Longus (EDL) and the Soleus, mostly composed of fast-twitch and slow-twitch fibres, respectively. Carbonylation is one of the most common oxidative modifications of proteins and a marker of oxidative stress. In fact, several studies suggest that physical activity and the consequent increase in oxygen consumption can lead to increase in reactive oxygen and nitrogen species (RONS) production, hence the interest in examining the impact of RONS on skeletal muscle proteins following ASE. Results indicate that protein expression is unaffected by ASE in both muscle types. Unexpectedly, the protein carbonylation level was reduced following ASE. In particular, the analysis found 31 and 5 spots, in Soleus and EDL muscles respectively, whose carbonylation is reduced after ASE. Lipid peroxidation levels in Soleus were markedly reduced as well. Most of the decarbonylated proteins are involved either in the regulation of muscle contractions or in the regulation of energy metabolism. A number of hypotheses may be advanced to account for such results, which will be addressed in future studies. [ABSTRACT FROM AUTHOR]
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- 2013
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5. Involvement of brain-derived neurotrophic factor and sonic hedgehog in the spinal cord plasticity after neurotoxic partial removal of lumbar motoneurons
- Author
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Gulino, Rosario and Gulisano, Massimo
- Subjects
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NEUROTROPHINS , *SPINAL cord diseases , *NEUROPLASTICITY , *NEUROTOXIC agents , *MOTOR neurons , *GENE expression - Abstract
Abstract: Adult mammals could spontaneously achieve a partial sensory-motor recovery after spinal cord injury, by mechanisms including synaptic plasticity. We previously showed that this recovery is associated to the expression of synapsin-I, and that sonic hedgehog and Notch-1 could be also involved in plasticity. The role of brain-derived neurotrophic factor and glutamate receptors in regulating synaptic efficacy has been explored in the last decade but, although these mechanisms are now well-defined in the brain, the molecular mechanisms underlying the so called “spinal learning” are still less clear. Here, we measured the expression levels of choline acetyltransferase, synapsin-I, sonic hedgehog, Notch-1, glutamate receptor subunits (GluR1, GluR2, GluR4, NMDAR1) and brain-derived neurotrophic factor, in a motoneuron-depleted mouse spinal lesion model obtained by intramuscular injection of cholera toxin-B saporin. The lesion caused the down-regulation of the majority of analysed proteins. Moreover, we found that in lesioned but not in control spinal tissue, synapsin-I expression is associated to that of both brain-derived neurotrophic factor and sonic hedgehog, whereas GluR2 expression is linked to that of Shh. These results suggest that brain-derived neurotrophic factor and sonic hedgehog could collaborate in modulating synaptic plasticity after the removal of motoneurons, by a mechanism involving both pre- and post-synaptic processes. Interestingly, the involvement of sonic hedgehog showed here is novel, and offers new routes to address spinal cord plasticity and repair. [Copyright &y& Elsevier]
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- 2012
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6. CD200 expression in patients with Multiple Myeloma: Another piece of the puzzle.
- Author
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Conticello, Concetta, Giuffrida, Raffaella, Parrinello, Nunziatina, Buccheri, Simona, Adamo, Luana, Sciuto, Maria Rita, Colarossi, Cristina, Aiello, Eleonora, Chiarenza, Annalisa, Romano, Alessandra, Salomone, Edvige, Gulisano, Massimo, Giustolisi, Rosario, and Di Raimondo, Francesco
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MULTIPLE myeloma , *CD antigens , *GENE expression , *CANCER invasiveness , *RAS proteins , *EXTRACELLULAR signal-regulated kinases - Abstract
Abstract: CD200 is a relatively ubiquitously expressed molecule that plays a role in cancer immune evasion through interaction with its receptors. High expression levels of CD200 have been described in different human malignancies. For example, CD200 has been shown to be targeted after RAS/RAF/MEK/ERK activation in melanoma. Here we present the analysis of CD200 expression in human Multiple Myeloma (MM) samples. We found that CD200-positive cells express ERK and p-ERK. Moreover, UO126, a MEK inhibitor, reduces CD200 expression. Furthermore, we observe that CD200-positive cells show reduced immunogenicity compared to normal lymphocytes and that such immunogenicity increases when UO126 is used. We therefore hypothesize that CD200 expression in MM could suppress antitumor response and that anti-CD200 treatment might be therapeutically beneficial in CD200-expressing tumors. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
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