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Using a rat model of peritonitis, we herein report the inflammatory effect induced by the lectin isolated from Vatairea guianensis (VGL) seeds in the context of interactions between VGL and both toll‐like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1). Peritoneal macrophages were stimulated with VGL for dose‐dependent gene expression and release of TNF‐α. In vivo results showed that VGL (1 mg/kg; intraperitoneal) induced peritonitis in female Wistar rats. Leukocyte migration, macrophage activation, and protein leakage were measured 3 and 6 hours after induction. In vitro, peritoneal macrophages were stimulated with VGL for gene expression and TNF‐α dosage (mean ± SEM (n = 6), analysis of variance, and Bonferroni's test (P <.05)). In silico, VGL structure was applied in molecular docking with representative glycans. It was found that (a) VGL increases vascular permeability and stimulates leukocyte migration, both rolling and adhesion; (b) lectin‐induced neutrophil migration occurs via macrophage stimulation, both in vitro and in vivo; (c) lectin interacts with TLR4 and TNFR1; and (d) stimulates TNF‐α gene expression (RT‐PCR) and release from peritoneal macrophages. Thus, upon lectin‐glycan binding on the cell surface, our results suggest that VGL induces an acute inflammatory response, in turn activating the release of peritoneal macrophages via TNF‐α and TLR and/or TNFR receptor pathways. [ABSTRACT FROM AUTHOR]

Summary: White tea derived from the Camellia sinensis (L.) plant has anticancer effects. Its main catechin decreases the gene expression of Vegf in tumours. However, VEGF is also the angiogenic factor of the reproductive organs. In this study, Wistar rats were divided into the following groups (n = 30): the control group (CT), which received water, and the white tea intake group (WT), for 3 months. At the end of every month, the corpora lutea (CL) of ten rats were removed to evaluate the gene expression of Vegf and its receptors (Flt‐1 and Kdr). White tea increased the relative mRNA abundance of Vegf (CT = 0.95 ± 0.06 and WT = 2.13 ± 0.19), Kdr (CT = 0.88 ± 0.06 and WT = 1.94 ± 0.32) and Flt‐1 (CT = 0.94 ± 0.06 and WT = 2.11 ± 0.22) in CL (P < 0.05). White tea increased angiogenic‐related genes in rat CL but did not alter the number of rat CL. [ABSTRACT FROM AUTHOR]