1. Insight into the effects of adipose tissue inflammation factors on miR-378 expression and the underlying mechanism.
- Author
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Jiang X, Xue M, Fu Z, Ji C, Guo X, Zhu L, Xu L, Pang L, Xu M, and Qu H
- Subjects
- Adipocytes cytology, Adipocytes drug effects, Adipocytes metabolism, Adipose Tissue metabolism, Binding Sites genetics, CCAAT-Enhancer-Binding Proteins metabolism, Cell Differentiation drug effects, Cell Differentiation genetics, Cells, Cultured, HEK293 Cells, Humans, Inflammation Mediators pharmacology, Interleukin-6 pharmacology, Promoter Regions, Genetic genetics, Reverse Transcriptase Polymerase Chain Reaction, Sterol Regulatory Element Binding Proteins metabolism, Tumor Necrosis Factor-alpha pharmacology, Cytokines pharmacology, Fatty Acids, Nonesterified pharmacology, Gene Expression drug effects, Leptin pharmacology, MicroRNAs genetics
- Abstract
Background/aims: Obesity and the related metabolic syndrome have emerged as major public health issues in modern society. miRNAs have been shown to play key roles in regulating obesity-related metabolic syndrome, and some miRNAs regulated by adiponectin were identified as novel targets for controlling adipose tissue inflammation. miR-378 is a candidate target that was shown to be involved in adipose differentiation, mitochondrial metabolism and systemic energy homeostasis. However, little is known about the regulatory mechanisms of miR-378 expression. To better understand the physiological role of miR-378 in obesity and metabolic syndrome, it is crucial that we understand the regulation of miR-378 gene expression in human adipocytes., Methods: In this study, we investigated the effects of adipokines and inflammatory cytokines on miR-378 expression using Real-time PCR and the potential regulatory mechanisms using luciferase reporter assays and electrophoretic mobility shift assay (EMSA). Results : We found that adipokines and cytokines upregulated miR-378 expression primarily through SREBP and C/EBP binding sites in the miR-378 promoter region., Conclusion: Our findings showed that adipokines induced miR-378 expression and revealed the most likely mechanism of adipokine-induced miR-378 dysregulation in human adipocytes. miRNAs have been shown to function in regulating obesity-related metabolic syndrome, and miR-378 may be a novel target for controlling adipose tissue inflammation. This study offers a theoretical basis for understanding systemic adipose tissue inflammation and may provide new strategies for clinical treatment., (© 2014 S. Karger AG, Basel.)
- Published
- 2014
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