1. Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk
- Author
-
You-Liang Zheng, Shi-Bin Gao, Bihua Wu, Yuming Wei, Xi-Gui Hu, and Dengcai Liu
- Subjects
Triticum timopheevii ,DNA, Complementary ,Physiology ,Single-nucleotide polymorphism ,Plant Science ,Biology ,Genes, Plant ,Exon ,Protein sequencing ,Species Specificity ,Nucleotide ,Amino Acid Sequence ,Gene ,Triticum ,Plant Proteins ,chemistry.chemical_classification ,Genetics ,Base Sequence ,Intron ,food and beverages ,biology.organism_classification ,Amino acid ,chemistry ,Seeds ,RNA ,Agronomy and Crop Science ,Transcription Factors - Abstract
NAM is an important domestication gene and valuable to enhance grain protein contents (GPCs) of modern wheat cultivars. In the present study, 12 NAM-G1 genes in Triticum timopheevii Zhuk. (AAGG, 2n = 4x = 28) were cloned. These genes had the same length of 1546 bp including two introns and three exons, and encoded a polypeptide of 407 amino acid residues which contained a N-terminal NAC domain with five sub-domains, and a C-terminal transcriptional activation region (TAR). They were highly similar to the previously published functional NAM-B1 gene DQ871219 from T. turgidum ssp. dicoccoides Korn. (AABB, 2n = 4x = 28) in both the nucleotide and protein sequence's, with a very high identity of 99.5%. The differences among the 12 NAM-G1 genes resulted from 17 SNPs including 14 transitions and 3 transversions. They had outstandingly different expression levels in qRT-PCR. And, their relative expression quantities were significantly positively correlated with GPC of the accessions. In addition, the difference in amino acid sequences of the NAM-G1 genes may also affect the GPC variation. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.
- Published
- 2013