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Your search keyword '"Gao, Albert E."' showing total 2 results
Start Over Author "Gao, Albert E." Topic gelatin
2 results on '"Gao, Albert E."'

1. In vitro and in vivo analysis of visible light crosslinkable gelatin methacryloyl (GelMA) hydrogels.

Author

Noshadi I, Hong S, Sullivan KE, Shirzaei Sani E, Portillo-Lara R, Tamayol A, Shin SR, Gao AE, Stoppel WL, Black LD III, Khademhosseini A, and Annabi N

Subjects
Animals, Caprolactam chemistry, Cell Proliferation drug effects, Male, Materials Testing, Mechanical Phenomena, Mice, Myocytes, Cardiac cytology, Myocytes, Cardiac drug effects, NIH 3T3 Cells, Polymerization, Rats, Rats, Sprague-Dawley, Biocompatible Materials chemistry, Biocompatible Materials pharmacology, Gelatin chemistry, Gelatin pharmacology, Hydrogels chemistry, Light, Photochemical Processes
Abstract

Photocrosslinkable materials have been frequently used for constructing soft and biomimetic hydrogels for tissue engineering. Although ultraviolet (UV) light is commonly used for photocrosslinking such materials, its use has been associated with several biosafety concerns such as DNA damage, accelerated aging of tissues, and cancer. Here we report an injectable visible light crosslinked gelatin-based hydrogel for myocardium regeneration. Mechanical characterization revealed that the compressive moduli of the engineered hydrogels could be tuned in the range of 5-56 kPa by changing the concentrations of the initiator, co-initiator and co-monomer in the precursor formulation. In addition, the average pore sizes (26-103 μm) and swelling ratios (7-13%) were also shown to be tunable by varying the hydrogel formulation. In vitro studies showed that visible light crosslinked GelMA hydrogels supported the growth and function of primary cardiomyocytes (CMs). In addition, the engineered materials were shown to be biocompatible in vivo, and could be successfully delivered to the heart after myocardial infarction in an animal model to promote tissue healing. The developed visible light crosslinked hydrogel could be used for the repair of various soft tissues such as the myocardium and for the treatment of cardiovascular diseases with enhanced therapeutic functionality.

Published
2017
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2. In vitro and in vivo analysis of visible light crosslinkable gelatin methacryloyl (GelMA) hydrogels

Author

Noshadi, Iman, Hong, Seonki, Sullivan, Kelly E, Shirzaei Sani, Ehsan, Portillo-Lara, Roberto, Tamayol, Ali, Shin, Su Ryon, Gao, Albert E, Stoppel, Whitney L, Black, Lauren D, Khademhosseini, Ali, and Annabi, Nasim

Subjects
Male, Light, Medical Biotechnology, Biocompatible Materials, Bioengineering, macromolecular substances, Cardiovascular, Regenerative Medicine, Polymerization, Mice, Medicinal and Biomolecular Chemistry, Materials Testing, Animals, Caprolactam, Cell Proliferation, Mechanical Phenomena, Myocytes, technology, industry, and agriculture, Hydrogels, Photochemical Processes, Rats, Heart Disease, NIH 3T3 Cells, Gelatin, Sprague-Dawley, Biochemistry and Cell Biology, Cardiac, Biotechnology
Abstract

Photocrosslinkable materials have been frequently used for constructing soft and biomimetic hydrogels for tissue engineering. Although ultraviolet (UV) light is commonly used for photocrosslinking such materials, its use has been associated with several biosafety concerns such as DNA damage, accelerated aging of tissues, and cancer. Here we report an injectable visible light crosslinked gelatin-based hydrogel for myocardium regeneration. Mechanical characterization revealed that the compressive moduli of the engineered hydrogels could be tuned in the range of 5-56 kPa by changing the concentrations of the initiator, co-initiator and co-monomer in the precursor formulation. In addition, the average pore sizes (26-103 μm) and swelling ratios (7-13%) were also shown to be tunable by varying the hydrogel formulation. In vitro studies showed that visible light crosslinked GelMA hydrogels supported the growth and function of primary cardiomyocytes (CMs). In addition, the engineered materials were shown to be biocompatible in vivo, and could be successfully delivered to the heart after myocardial infarction in an animal model to promote tissue healing. The developed visible light crosslinked hydrogel could be used for the repair of various soft tissues such as the myocardium and for the treatment of cardiovascular diseases with enhanced therapeutic functionality.

Published
2017
Full Text
View/download PDF

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