1. Immunological activities of polysaccharide extracted from Elaeagnus angustifolia L.
- Author
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Sun, Na-xin, Liu, Hui-ping, Liu, Xu-hui, Zhang, Yan, Liu, Xiao-qing, Wang, Shu, Xu, Xiang-xin, and Tian, Wen-tan
- Subjects
POLYSACCHARIDES ,RUSSIAN olive ,GEL permeation chromatography - Abstract
The study was to investigate the immunological activities of polysaccharide isolated from Elaeagnus angustifolia L. The extracted Elaeagnus angustifolia L. polysaccharide (EAP) was purified by DEAE cellulose-52 chromatography (DEAE-52) and Sephadex G-100 size-exclusion chromatography (G-100). Then the average molecular weight (Mw) of purified polysaccharide was determined by high-performance gel permeation chromatography (HPGPC). One main fraction (EAP-1a) was obtained through the isolation and purification steps. The results showed that EAP-1a had strong immunological activities. EAP-1a could significantly increase spleen and thymus index, enhance the NK cell activity, stimulate the phagocytosis of peritoneal phagocyte and increase the level of IL-2, IFN-γ and IgG in serum. Thus, EAP-1a isolated from Elaeagnus angustifolia L. could enhance the immunological activities of immunosuppressed mice induced by cyclophosphamide (CY). [ABSTRACT FROM AUTHOR]
- Published
- 2018
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