1. Solid-state NMR characterization of gas vesicle structure.
- Author
-
Sivertsen AC, Bayro MJ, Belenky M, Griffin RG, and Herzfeld J
- Subjects
- Anabaena, Hydrophobic and Hydrophilic Interactions, Movement, Protein Structure, Secondary, Proteins chemistry, Proteins metabolism, Solvents chemistry, Gases, Magnetic Resonance Spectroscopy methods, Organelles chemistry
- Abstract
Gas vesicles are gas-filled buoyancy organelles with walls that consist almost exclusively of gas vesicle protein A (GvpA). Intact, collapsed gas vesicles from the cyanobacterium Anabaena flos-aquae were studied by solid-state NMR spectroscopy, and most of the GvpA sequence was assigned. Chemical shift analysis indicates a coil-α-β-β-α-coil peptide backbone, consistent with secondary-structure-prediction algorithms, and complementary information about mobility and solvent exposure yields a picture of the overall topology of the vesicle subunit that is consistent with its role in stabilizing an air-water interface., (Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF