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ABSTRACT This study aims to comparatively analyze butter, ghee, and margarine fatty acid profiles and their implications for healthier fat and oil group choices. In this cross‐sectional study, 60 samples from best‐selling brands of butter, ghee, and margarine were randomly selected from five food chain stores in Tehran, Iran. Then, all the samples were coded, packed in cool conditions, and sent to the laboratory to determine the fatty acid profiles by using gas chromatography (GC). Based on the authors' experiences and the available literature, a policy dialogue session was held with stakeholders about oil and fat challenges, followed by a Strengths, Weaknesses, Opportunities, and Threats (SWOT) analysis. The mean ± SE of total fatty acids in butter, ghee, and margarine was 94.07 ± 0.17, 94.49 ± 0.61, and 99.00 ± 0.18; total saturated fatty acid (TSFA) 66.69 ± 0.39, 64.26 ± 0.63, and 40.36 ± 0.87; trans fatty acid (TFA) 2.43 ± 0.09, 3.60 ± 0.29, and 0.83 ± 0.15 g/100 gfat, respectively. The predominant TFAs in butter and ghee were vaccenic acid (animal source) (2.06 ± 0.07 and 2.41 ± 0.17), while in margarine, it was elaidic acid (plant‐based source) (0.32 ± 0.12 g/100 gfat), respectively. Also, the SWOT findings showed being TFAs in the acceptable range (as the main strength), mismanagement (Weakness), reducing taxes based on lower TFA content (Opportunity), and sanctions (as the main Threat) were the most important criteria affecting fat choices in the Iranian food basket. The results of the study found that butter, ghee, and margarine contain relatively low levels of TFAs; however, butter and ghee were rich in beneficial fatty acids, which have been shown to have health‐protective properties. Policymakers can implicate the proposed strategies and opportunities from the SWOT analysis for healthier fat and oil choices to promote public health. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Edible crickets have recently been used as a new alternative protein source with high nutritional value. The nutritional and flavor-related value of edible crickets varies greatly depending on the species, growth conditions and processing conditions. However, few studies have investigated the effects of the diet fed to crickets during their growth phase on flavor. Therefore, in this study, we characterized the flavor-related factors of powder from crickets reared on apple by-products (ACP) by comparing them with those of powder from crickets reared on a control diet (CCP). The fatty acid composition and volatile compounds of each powder were determined using gas chromatography and mass spectrometry, followed by sensory analysis and color measurement. A decrease in unsaturated fatty acids, specifically γ-linolenic acid, α-linolenic acid, arachidonic acid and docosahexaenoic acid, was observed in ACP. A total of 50 volatile compounds were identified, of which 11 were present in only ACP, while 39 were found in both powders. The sensory analysis showed that the overall balance score of ACP was higher than that of CCP, and according to the color measurements, ACP was darker than CCP. These differences between CCP and ACP might have been due to the differences in the chemical composition of the diets fed to the crickets during their growth phase. The results of this study suggest that one of the factors determining the food value of edible crickets, especially in terms of flavor, is the diet they are fed during their growth phase. [ABSTRACT FROM AUTHOR]

Recently, the use of bone grafts increased in medical practice. Some bone grafts may have different mechanical and biological properties of natural bone. To improve bone grafts we need to record our knowledge about bone. In this work, we are interested in determining the chemical composition of bone fluid in the knee joint as it is a lipid-predominant fluid. Bone samples were obtained from knee cancellous bone. Samples were used of women aged 60 years old with osteoarthritis. Sample preparation is essential to guarantee the quality and dependability of the analysis results. Bone fluid was centrifuged for 15 minutes at 3000 rpm to eliminate the blood and debris. The supernatant was then gathered and filtered. Extraction of total lipids from the bone fluid according to the method of Bligh and Dyer (1959) with modification. The bone fluid of a fresh human cancellous bone was extracted by mechanical compression tests. It consists of 90% lipids. The fatty acid composition of the bone fluid was determined by gas chromatography. Methyl oleate is the most abundant. [ABSTRACT FROM AUTHOR]

The major fatty acids present in cosmetics are the unsaturated fatty acids from triglycerides, especially essential fatty acids: linoleic acid (omega-6) and a-linolenic acid (omega-3). The purpose of the study was to develop a simple and precise gas chromatography-flame ionization detection method, using an OPTIMA-WAX (macrogol 20000) capillary GC column (30m x 0.32mm x 0.25µm) with a run time of 17min, for the analysis of fatty acids composition from vegetable oils and macerated oils. The method was validated for quantifying four major fatty acids: palmitic, stearic, oleic and linoleic acids, as methyl esters. The quantification was performed by internal standardization, using the methyl ester of nonadecanoic acid as internal standard. The esterification reaction was carried out on a magnetic stirrer at a temperature of 80°C and with continuous stirring, in hermetically sealed vials. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agriculture & Nature / Kahramanmaraş Sütçü İmam Üniversitesi Tarım & Doğa Dergisi is the property of Kahramanmaras Sutcu Imam Universitesi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Lychnis coronaria is a plant of Caryophyllaceae family, its raw material has been used in folk medicine of many countries as anti-inflammatory, wound healing and anti-haemorrhoidal drug. Objective: The study concerns fatty acid composition of Lychnis coronaria herb, leaves, flowers, stem and seeds. The raw material was collected in Kharkov Region, Ukraine. Methods: Fatty acids were studied with gas chromatography (GC) after their transformation to methyl esters. Results: The performed experiment showed that in all types of analysed raw material unsaturated fatty acids prevailed quantitatively. Among unsaturated fatty acids, linoleic acid dominated in herb, flowers, stems and seeds, whereas linolenic acid prevailed in leaves. Palmitic acid was predominant among saturated fatty acids in all types of Lychnis coronaria raw material. Conclusion: Thus, our research brings a substantial contribution to study of Lychnis coronaria raw material chemical composition, and the obtained results may be applied in the development of new medicines based on this plant. [ABSTRACT FROM AUTHOR]

Objectives: Post-marketing/surveillance studies show that most of the many vegetable oils that are sold with health-promoting claims or statements with high nutritional values and are beneficial against diseases are off-limits of related monographs/criteria. Defining the oil with a fast, cheap, and efficient analytical method is needed to express fatty acids in any herbal product to authenticate, trace, specify, and classify the content. Materials and Methods: Here, we define a new simple tool with a headspace single drop microextraction (HS-SDME) method coupled with a gas chromatography-flame ionization detector (GC-FID) for the analysis of common fatty acids (FAs) in oils. Linolenic acid, γ-linolenic acid, and linoleic acid in olive oil, thyme oil, and fish oil were determined. Derivatization was performed with 0.2 mL of 2 mol/L KOH in methanol to transfer the FAs of oils into their methyl esters (FAMEs). Then, FAMEs were extracted using a head space single drop, which is 2.0 µL of sodium dodecyl sulfate: 1-butanol (1:3, v/v) mixture. Results: The most suitable extraction condition was that 360 µL of the FAMEs, 2.0 mL vial, 0.07 g NaCl as a salting-out effect, 45 °C extraction temperature, and 35 min extraction time. The precision of the method was below 12%, with accuracy validated by the GC-FID reference method. Conclusion: The HS-SDME can be used effectively for extracting FAs from oils for improved analysis of other FAs. The method is of direct importance and relevance for the herbal, pharmaceutical, and cosmetics industries. [ABSTRACT FROM AUTHOR]

Fatty acids have a crucial role in providing energy and performing essential functions in living organisms. Moreover, these substances exhibit the most significant alterations in their structure based on ecotoxicological parameters when viewed from a biochemical perspective. These bioactive chemicals are present in the cellular architecture. The study of these fatty acids, crucial for maintaining the integrity and permeability of cell membranes, holds great significance for all living organisms. Consequently, doing fatty acid analysis specifically at the phospholipid level holds significant importance. The impact of lambda cyhalothrin on the fatty acid content of several phospholipid subclasses (phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS) in the gill tissue of O. niloticus (Perciformes: Cichlidae) was assessed using gas chromatography. The alterations in the fatty acid composition was analyzed 21 days after exposure. Following the complete extraction of lipids from gill tissue, the tissue was subsequently separated into different subclasses of phospholipids using thin layer chromatography. The samples were subjected to methylation and then evaluated using Gas Chromatography to determine the percentage of the fatty acid. After doing the analysis, a grand total of 16 fatty acids were identified. The research revealed that the primary fatty acids were 16:0 and 18:0 of saturated fatty acids, monounsaturated 18:1n-9, and polyunsaturated 18:2n-6, 20:4n-6, and 22:6n-6. Upon analyzing the distribution of fatty acids, it was observed that PC, PE, and PI included 16:0, PE contained 18:1, PE and PS contained C18:2n-6 and 20:4n-6, and significant alterations in C22:6n-3 were detected in PE. Our investigation revealed that the n-3/n-6 ratio of fish in the PE subclass was the lowest when compared to PC, PI, and PS. [ABSTRACT FROM AUTHOR]

This study examined the presence of aflatoxins, fatty acid compositions, and storage stability in cookies (BSC), digestive biscuits (BSD), and crackers (BScr) produced in different countries. The findings showed that AFB2 and AFG2 were not found in the studied biscuits, but AFB1 was detected in BSD1-S, BSD1-I, BSC2-U, and BScr1-T, while AFG1 was only found in BSD1-S and BScr1-T. The saturated fatty acid content in BSC, BSD, and BScr samples varied from 20.69% to 45.90%, 10.90% to 47.03%, and 11.35% to 46.88%, respectively. The unsaturated fatty acid content in BSC, BSD, and BScr samples ranged from 11.31% to 59.17%, 12.40% to 74.24%, and from

This study aimed to detect the adulteration of different types of soft cheese with vegetable oils. Therefore, a total of 36 samples (6 for each) of Talaga, Kiri spread, Baramili spread, Baramili, and white and yellow feta cheese were collected from local markets in Damietta governorate, Egypt. The samples were evaluated for their chemical composition, assessed their fatty acid profile using gas chromatography, and estimated their peroxide value. The chemical evaluation revealed that all Talaga, Baramili, white and yellow feta cheese samples were matching the Egyptian standards while all Kiri and Baramili spread samples didn't match. By comparing the fatty acid profile of the control cow milk with that of the examined cheese samples, there were differences in certain FAs values demonstrating the fat impurity in the cheese samples. 83% of Talaga samples and all other cheese types were adulterated with vegetable oils. The saturated FA decreased, unsaturated FA increased and the peroxide value elevated, conversely from the situation in control milk, that happened in 50% of Talaga and white feta, and 100% of other types. In conclusion, FA profile estimation could be an efficient detection method for vegetable oil adulteration in dairy products. [ABSTRACT FROM AUTHOR]

This study developed a method for quantifying eight short-chain fatty acids (SCFAs) in mouse fecal samples using solid-phase microextraction (SPME) coupled with triple quadrupole gas chromatography tandem mass spectrometry. Furthermore, significant factors affecting SCFA analysis, including SPME fiber selection, pH, salting-out agent, and sample collection time, were investigated. Contrary to previous studies, we found that the CAR/PDMS fiber had the highest extraction efficiency for all SCFAs. The optimal extraction efficiency was observed at pH 2.0, particularly for low-molecular-weight SCFAs. NaH2PO4 showed a more effective extraction efficiency than NaCl, owing to its pH stability and less interference with the solvent matrix. Additionally, our results showed that the SCFA concentration increased over collection time. The composition ratio of the eight SCFAs was maintained for up to 24 h; thus, we concluded that samples should be collected within four hours to obtain reliable results. Our findings may improve laboratory methods for SCFA extraction and mouse fecal sample analysis. [ABSTRACT FROM AUTHOR]

This study aims to investigate the impact of anchovy (Engraulis encrasicolus) oil produced in different factories on the fatty acid composition and quality indicators. The study utilizes anchovy oils obtained from three different factories. Fatty acid analysis was conducted using gas chromatography, and the results were expressed as percentages. Additionally, fatty acid quality indices such as atherogenic index (AI), thrombogenic index (TI), Polyene index (PI), and hypocholesterolemic/hypercholesterolemic ratio (h/H) were calculated. The findings of the study indicate that different processing technologies may influence the fatty acid composition of anchovy oil. Anchovy oils were observed to be rich in polyunsaturated fatty acids (PUFA) and notably contain omega-3 fatty acids such as DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid). This study provides valuable insights into anchovy oil production and quality, offering an in-depth understanding of sustainable nutrition. In conclusion, this study sheds light on a significant issue in the anchovy oil industry and may guide researchers and industry experts interested in improving the quality of fish oil products and supporting human health with potential opportunities. [ABSTRACT FROM AUTHOR]

Background and Objectives: The quantity of absorbed oil in fried foods is a concern issue due to the presence of unhealthy trans fatty acids and alteration of nutrients and consequently their effects on public health. Therefore, the aim of the present study was to investigate fat contents of the guild and industrial fried foods in Iran and compare these content values with those of the national standards. Materials and Methods: In this study, total fat, saturated and trans fatty acid contents of 142 industrial (potato chips, shoestring potatoes and onions) and guiled (potato chips, potatoes, onions and garlics) fried foods from various regions of Iran in two consecutive years were assessed. First, Soxhlet extraction was used to extract fats. Then, all free acids were methyl-esterified and detected using gas chromatography. Compliance of total fat, saturated fatty acid and trans fatty acid contents of each fried food with Iranian national standards (INS) was assessed. Significant differences between the means of fatty acid composition of the fried foods were reported using ANOVA and Duncan post hoc tests (p < 0.05). Results: The mean fat concentrations of industrial (41.30%) and guild potato chips (42.89%) in 2017 and industrial potato chips (38.83%) in 2019 were higher than the Iranian national standards (38% w/w). The mean of saturated and trans fatty acids in the oily phase of guild potato chips was statistically different in 2017 and 2019 (p < 0.05). Significant differences were seen between the quantities of saturated fatty acids in the oily phase of shoestring potatoes in 2017 and 2019, while no significant differences were seen between the total fat, saturated and trans fatty acids of guild-fried potatoes in 2017 and 2019 (p < 0.05). Furthermore, levels of trans fatty acids in the oily phase of industrial fried onions (2.23%) and guild-fried garlics (2.41%) in 2019 were above the Iranian national standards (2% w/w). Conclusions: It seems that actions are needed to decrease the absorbed fats by fried products. [ABSTRACT FROM AUTHOR]

The fatty acid profile of cheese influences its sensory parameters, such as color, texture, or flavor. Examining the fatty acid profile also helps to assess the nutritional value of the cheese that is being tested. However, the determination of fatty acids in cheese samples is a multi-stage and time-consuming task. In addition, large amounts of toxic organic solvents are used to prepare samples for analysis purposes. This paper presents the results of a study to determine the fatty acid profile of yellow cheese samples. Six different methods of sample preparation were compared for analysis purposes. The profile of fatty acids was determined using gas chromatography with flame ionization detection (GC-FID). The study showed significant differences (p > 0.05) in the resulting fatty acid profile between the methods used. It was found that the most reliable fatty acid profile results were obtained using methods derived from the Folch method. In addition, tools such as the Analytical Eco-Scale tool and the Analytical Greenness Metric for Sample Preparation (AGREEprep) tool were used to assess the greenness of the methods used. In the case of the Analytical Eco-Scale tool, all six methods scored 'acceptable green analysis' with scores ranging from 61 to 73. However, an evaluation of methods using the AGREEprep metric showed that the results of the methods (0.13–0.27) did not show the "greenness" of the analytical methods. [ABSTRACT FROM AUTHOR]

Here, we present the results of analyzing the fatty acid composition of the main edible vegetable oils from Kazakhstani oilseed producers (safflower, sunflower, maize (corn), linseed, cottonseed, soybean and rapeseed) in comparison with the known fatty acid (FA) composition of specific vegetable oils complying with the Codex Standard for Named Vegetable Oil (Codex Stan 210-1999). The fatty acid composition of 35 different vegetable oils was analyzed by gas chromatography with a Shimadzu GC-2010 Plus instrument with flame ionization detection using a high-polarity CP-Sil 2560, which allowed us to establish their authenticity for high accuracy and excellent separation. A comparative study of the fatty acid composition, groups and omega-6/omega-3 ratios in seven different vegetable oils was carried out. Subsequently, the data were processed by hierarchical clustering analysis, principal component analysis, artificial neural network and Pearson's correlation. Artificial neural network analysis demonstrated correct predictions. Principal component analysis showed the effects of oleic, linoleic and α-linolenic acids to vegetable oils classification. [ABSTRACT FROM AUTHOR]

The global demand for good quality food is going to be increased gradually. Mushrooms are broadly used as healthy nutritious meals. The nutritional values of extracts from four distinct Pleurotus species—Pleurotus ostreatus, Pleurotus sajor-caju, Pleurotus sapidus, and Pleurotus columbinus—were determined in the current study. Firstly, proximate analysis of selected Pleurotus species was performed followed by the Bradford assay to analyze the protein spectrophotometrically; high-performance liquid chromatography (HPLC) was performed for sugar determination while GC-MS was done to determine fatty acids on organic extracts of selected mushrooms. Descriptive statistics were used to calculate the percentages while significance was determined by SPSS statistics. The results depicted that fat, protein, ash, fiber, energy contents, and total carbohydrate were in the range of 0.64-2.02%, 16.07-25.15%, 2.1-9.14%, 6.21-54.12%, 342.20-394.30 kcal/100 g, and 65.66-82.47%, respectively. The protein's maximum concentration was observed in P. ostreatus followed by P. columbinus>P. sajor-caju>P. sapidus, sequentially. Various sugars may or may not be present in selected Pleurotus spps. Among the fatty acids, the prevalence of UFA was more than that of saturated fatty acids among all selected mushrooms. From this study, it is concluded that all four Pleurotus spps. have excellent nutritional composition and can be used as valuable food and a great source of biochemical compounds. [ABSTRACT FROM AUTHOR]

Background: Chromatography permits chemical components' separation, identification, and quantification in complex mixtures. Chromatographic methods have various applications, which include the determination of an agrochemical solvent percentage and its utilization in the food-processing industry. High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are essential for agriculture. Both types are used in numerous research studies today thanks to their versatility, sensitivity, reproducibility, and quickness. Aim: To discuss the main results of studies that include GC and HPLC in agriculture. Materials and methods: The study relied on a compilation of recent papers, where these techniques play a critical role in this area of science. Results: The utilization of GC and HPLC in agriculture contributed to relevant results, from the analysis of fatty acids profile in the formulation of a diet for animal nutrition, to the determination and quantification of agrochemical residues in plants, the detection of contaminants in the diets, and drug residues that compromise the lives of animals. Conclusions: The use of chromatography is an essential methodology for biochemical, toxicological, structural, and agro-industrial studies. [ABSTRACT FROM AUTHOR]

Copyright of @limentech: Ciencia y Tecnología Alimentaria is the property of Journal @limentech, University of Pamplona and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Determination of the fatty acid profile in milk samples is one of the most important in food analysis. There are many methodologies for FA determination. The conventional procedure for determining the FA composition of milk is isolation of fat or indirect methylation, trans-methylation, extraction of fatty acids, and analysis by gas chromatography. In this study, eight methods based on alkaline methylation were compared for the analysis of fatty acids in cow's milk. The response factors (RF) for GC analysis using FID were calculated. For most acids, RFs were close to 1, with the exception of short-chain fatty acids (C4:0–C8:0). To facilitate the selection of the method for the determination of fatty acids in milk samples, the methods were assessed using the environmental assessment tools of the analytical procedure: the Analytical Eco-Scale, Green Analytical Procedure Index (GAPI), and Analytical Greenness for Sample Preparation (AGREEprep). The method based on direct milk methylation received the highest scores. Omitting the lipid separation step has an impact on reducing the quantity of used toxic chemicals and reagents, and produces a smaller amount of waste, a much higher throughput, and a reduced cost analysis. [ABSTRACT FROM AUTHOR]

A new analytical method for the determination of six volatile short and medium-chain fatty acids (acetic, propionic, isobutyric, isovaleric, hexanoic, and octanoic acids) through liquid–liquid extraction with diethyl ether, followed by GC-FID analysis, was developed and validated. The extraction conditions were optimized by evaluating the effect of the number of extractions (1 to 3) and the effect of the addition of salts (NaH2PO4, (NH4)2SO4, NaCl, (NH4)2SO4/NaH2PO4) to increase the concentration of the analytes in the ethyl ether phase. Results showed that a single extraction allows obtaining the highest sensitivity (due to the impossibility of evaporating the solvent to avoid losses of the analytes). The use of salting out agents, in particular, NaH2PO4, showed an important increase in the extraction extent, on average, 1.5 times higher as compared to the extraction performed without salt. The proposed method is rapid, requiring a total of 30 min for preparation and analysis, and it makes use of small amounts of sample (500 µL) and solvent (400 µL). The method was then applied to quantify the analytes in 5 white wines and 5 red wines, allowing to highlight some clear differences between red and white wines, with the red ones having a significantly higher amount of acetic acid (715.7 ± 142.3 mg/L in red wines and 351.5 ± 21.2 mg/L in white wines) and the white wines having a significantly higher amount of hexanoic and octanoic acid (6.1 ± 3.0 mg/L and 2.6 ± 0.8 mg/L, respectively, are the mean concentrations in white wines, and 4.7 ± 0.8 and 2.4 ± 0.4 mg/L, respectively, are the mean concentrations in red wines). [ABSTRACT FROM AUTHOR]

Copyright of Food & Machinery is the property of Food & Machinery Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Because of the central role of fatty acids in biological systems, their accurate quantification is still important. However, the impact of the complex matrix of biologically and clinically relevant samples such as plasma, serum, or cells makes the analysis still challenging, especially, when free non-esterified fatty acids have to be quantified. Here we developed and characterized a novel GC–MS method using pentafluorobenzyl bromide as a derivatization agent and compared different ionization techniques such as atmospheric pressure chemical ionization (APCI), atmospheric pressure chemical photoionization (APPI), and negative ion chemical ionization (NICI). The GC-APCI-MS showed the lowest limits of detection from 30 to 300 nM for a broad range of fatty acids and a similar response for various fatty acids from a chain length of 10 to 20 carbon atoms. This allows the number of internal standards necessary for accurate quantification to be reduced. Moreover, the use of pentafluorobenzyl bromide allows the direct derivatization of free fatty acids making them accessible for GC–MS analysis without labor-intense sample pretreatment. [ABSTRACT FROM AUTHOR]

Objectives: Datura stramonium L., D. stramonium var. tatula (L.) Torr., Hyoscyamus reticulatus L., H. niger L., and Atropa caucasica Kreyer naturally found in Azerbaijan and their seeds possess 17-35% of oils. This study aims to evaluate and determine fatty acids of these plants special to Azerbaijan climate and geography. The presented study is the first to research into fatty acids of A. caucasica, which is an endemic species of Caucasus. Materials and Methods: Fatty acid seed oils were derivatized to methyl esters and analyzed by gas chromatography equipped with a flame ionization detector, compared with a standard mixture of 37 fatty acid methyl esters. Results: Linoleic (55-79%), oleic (11-26%), palmitic (4-12%), and stearic (2-3%) acids compose 97% of total fatty acids. Other minor compounds, including two trans-fatty acids, were determined in the samples. Significantly high concentration of a medicinally important polyunsaturated fatty acid, e.g. linoleic acid, was observed in all samples. Conclusion: The results of this study showed that these oils are particularly valuable sources of linoleic and oleic acids, which have beneficial effects on cardiovascular diseases and are important compounds for the pharmaceutical and cosmetic industries in the manufacture of liposomes, nano- and microemulsions, soaps, etc. [ABSTRACT FROM AUTHOR]

To elucidate the medicinal nature of black ginseng (BG) by comparison of the effects of four Chinese herbs with different medicinal natures on the deficiency-heat syndrome rat model which was established by intragastric administration of traditional Chinese drugs with hot nature, the appearance indexes, biochemical indexes, and pathological sections of thyroid and stomach were examined. In addition, the seven short-chain fatty acids (SCFAs) in rat feces were also determined by headspace gas chromatography-mass spectrometry to reveal the action mechanism of the drugs with different natures. Results indicated that all the 4 drugs could exhibit similar actions in regulating the biochemical indexes of triiodothyronine (T3), thyroxine (T4), thyrotropin-releasing hormone (TRH), thyroid-stimulating hormone (TSH), and corticosterone (CORT) representing the hypothalamus-pituitary-thyroid (HPT) and hypothalamus-pituitary-adrenal (HPA) axes of the animal. However, cold-natured cortex phellodendri (HB) and ginseng leaves (GLs) showed stronger downregulation of the AChE activity of the nervous system. Red ginseng (RG) and BG tested exhibited stronger upregulation of the liver Na+-K+-ATPase activity. Principal component analysis (PCA) showed that GLs are similar to those of HB which belongs to the cold-nature drug, whereas BG showed closer to RG which attributes to a warm-nature drug. Thus, BG could be ascribed to a warm-nature drug. Further research disclosed that RG and BG mainly regulated the acetic acid and GL and HB primarily modulated the isovaleric acid and hexanoic acid in rat feces, which could be the features of drugs with warm or cold nature on the regulation of SCFAs in rats. It is for the first time that the medicinal nature of BG and its effect on the SCFAs were examined. [ABSTRACT FROM AUTHOR]

The common carp (Cyprinus carpio) is the main species of carp being cultured in mid and southern Iraq. The consumer believes that the farmed fish is less nutritious than the wild fish, which affects its market value. The current study was designed to investigate the nutritional value of the common carp cultivated in the Basrah province based on the fatty and amino acid profile. Fatty acids were determined by Gas chromatography (GC), while an Amino Acid Analyzer was used for the determination of amino acids. The results revealed that the tested fish is rich in polyunsaturated fatty acids (especially essential omega-3 fatty acids), including a-linolenic acid (14.08±3.92%), docosahexaenoic acids (7.98 ±2.82 %), and eicosapentaenoic acid (2.81 ±0.34 %). Seven essential amino acids which are significant for the human body and the other ten non-essential amino acids were identified in the samples tested. Thus, it was established that the nutritional value of the farmed Cyprinus carpio is not less than the wild fish of the same species or family. [ABSTRACT FROM AUTHOR]

Apple industrial by-products are a promising source of bioactive compounds with direct implications on human health. The main goal of the present work was to characterize the Jonathan and Golden Delicious by-products from their fatty acid, amino acid, and volatile aroma compounds' point of view. GC-MS (gas chromatography-mass spectrometry) and ITEX/GC-MS methods were used for the by-products characterization. Linoleic and oleic were the main fatty acids identified in all samples, while palmitic and stearic acid were the representant of saturated ones. With respect to amino acids, from the essential group, isoleucine was the majority compound identified in JS (Jonathan skin) and GS (Golden skin) samples, lysine was the representant of JP (Jonathan pomace), and valine was mainly identified in GP (Golden pomace). A total number of 47 aroma volatile compounds were quantified in all samples, from which the esters groups ranged from 41.55–53.29%, aldehydes 29.75–43.99%, alcohols from 4.15 to 6.37%, ketones 4.14–5.72%, and the terpenes and terpenoids group reached values between 2.27% and 4.61%. Moreover, the by-products were valorized in biscuits manufacturing, highlighting their importance in enhancing the volatile aroma compounds, color, and sensorial analysis of the final baked goods. [ABSTRACT FROM AUTHOR]

The review considers the principal, as well as less common but promising, methods to determine the fatty acids (FAs) in biological samples. The advantages, disadvantages, and prospects of these methods are described and compared. Special attention is paid to specific features of the preparation of biological samples, such as the extraction of lipids and derivatization process of FAs. Methodological aspects of the determination of FAs via gas chromatography (GC) and high-performance liquid chromatography (HPLC) and features of the applications of various types of stationary phases used for the chromatographic separation of FAs are considered. The potential for the use of capillary electrophoresis (CE) and nuclear magnetic resonance spectroscopy (NMR) to analyze FAs are analyzed. The special requirements and limitations of these methods concerning work with biomaterials are also considered [ABSTRACT FROM AUTHOR]

Soybean oil is the most consumed oil worldwide due to its cheapness but presented a weak thermo-resistance due to its richness in poly-unsaturated fatty acids. This study aims to improve the thermo-stability of refined soybean oil by blending it with some cold-pressed oils. For this, cold-pressed and soybean oils were firstly characterized (K232, K270, acidity, peroxide value, iodine value, induction time, phenolic contents, and antioxidant activity). Then, binary blends of each cold-pressed oil (30%) with soybean oil (70%) were analyzed before and after heat treatment (170 °C for 10 h/day for 5 days) followed by the application of the simplex lattice mixture design in order to optimize the combination of the three best cold-pressed oils. The changes in fatty acid profiles were assessed by gas chromatography (GC-FID). The results revealed that soybean oil presented the best physicochemical traits, while cold-pressed oils expressed high levels of phenolic contents and antioxidant activities. From the six binary oil blends, soybean oil mixed with lentisk, sesame, or almond oils were selected for their best thermo-stability. The simplex lattice mixture design, applied for these three chosen oils, indicated that the combination of soybean oil (70%) with lentisk and sesame oils (17.7 and 12.3%, respectively) was considered the optimal blend that gives the maximal thermo-stability improvement to soybean oil. GC-FID analysis showed that fatty acids, particularly linoleic and linolenic acids, were more conserved after heat-treatment in optimal oils blend than soybean oil. This study clearly demonstrated that lentisk and sesame oils enhanced the thermo-resistance of soybean oil, and the findings of this study could be used as an integrated model in oil and fat industries. [ABSTRACT FROM AUTHOR]

Human milk lipids are among the many nutrients delivered to the infant, providing >50% of the infant's calorie intake. These lipids are highly complex and variable, and bioactive, contributing to infant growth, development, and health. The lipid concentration of milk samples is often measured in human cohorts; however, few studies measure infant intake of milk. Intake is important because it considers the variability of both lipid concentration and infants' consumed volume of milk. Measurement of infants' lipid intake in exclusively breastfeeding infants requires 3 main considerations: human milk sampling protocol (ie, the collection of representative samples); measurement of the infant milk intake, because volume varies widely between infants; and appropriate analytical laboratory methods. The purpose of this review was to provide an overview of existing methodology and demonstrate the importance of measuring infants' lipid intake to understand the impact that human milk lipids have on infant outcomes. [ABSTRACT FROM AUTHOR]

Background: Cocoa butter (CB), an expensive product containing a specific triacylglycerol (TAG)composition, is often the object of economic adulteration. Objective: The present study investigates the detection and quantification of cocoa butter substitutes (CBS) using differential scanning calorimetry. Method: CBS was mixed with CB (from 0 to 100%) as standard samples and analyzed using differential scanning calorimetry and gas chromatography to evaluate the thermal characteristics and fatty acid composition respectively. Results: Increasing the ratio of CBS/CB changed the thermal parameters and fatty acid profiles significantly. For quantification of CBS in commercial samples, medium melting fraction temperature (TMMF via r² = 0.922) and melting enthalpy (ΔHf via r² = 0.972) were successfully used to quantify the value of CBS in the samples suspected of being fraudulent. According to the results, all of the thermal parameters increased significantly (P < 0.05) with increasing ratio of CBS/CB. It can be attributed to the presence of saturated fatty acids (C12-C22) in CBS which increases TLMF. Conclusions: The promising results of the present study could be used as a tool for the fast identification of adulteration and quantification of CBS in chocolate compositions. Highlights: Differential scanning calorimetry methods has been developed for identification of CB adulteration. CBS adulteration was detected in CB and also in commercial chocolate samples. [ABSTRACT FROM AUTHOR]

The aim of this work was to study the quality (oxidative stability), fatty acids (FAs) profile and their distribution in triacylglycerol (TAG) molecules of hunted game wild fat (HGWF) obtained from wild boar, badger, and wild goose. Health lipid indices were calculated. The determination of FAs composition was carried out by gas chromatographic analysis of fatty acid methyl esters (FAME). Enzymatic hydrolysis and thin layer chromatography were used to determine the positional distribution of FAs in the internal and external positions of triacylglycerols (the regiospecific structure of triacylglycerols) of the obtained HGWF. A pressure differential scanning calorimeter (PDSC) was used to determine the oxidative stability of tested HGWF. The lipid fraction isolated from subcutaneous adipose tissues was dominated by MUFA, on average 46–50% of total FAs, and by SFA, on average 32–36% of total FAs. Palmitic acid was located mainly in the internal position of TAGs of the analyzed HGWF, whereas external positions were occupied by unsaturated oleic acid. Such a structure is responsible for normal fat absorption from food and it prevents the formation of insoluble calcium salts. Considering FAs profile, quality and the oxidation stability among all tested fat samples, the boar fat seems to be the most favorable. [ABSTRACT FROM AUTHOR]

Background Infants born at very low birth weight (VLBW) are vulnerable to deficits in fatty acids (FAs) but little is known of factors that influence the intakes or composition of their human milk feeds. Objectives We aimed to identify sources of variability in the fat composition of human milk fed to VLBW infants and examine the impact of milk source (mother's own or donor) on fat and FA intakes. Methods Serial samples of mother's milk (n  = 476) and donor milk (n  = 53) fed to infants born weighing <1250 g (n  = 114 infants from 100 mothers) were collected [Optimizing Mothers' Milk for Preterm Infants (OptiMoM) randomized clinical trial]. Fat and FA were analyzed using a mid-infrared human milk analyzer and GC with flame ionization detection. Results At full enteral feeding, donor milk is estimated to provide 1.3 g · kg−1 · d−1 less total fat than mature mother's milk (recommended intake: 4.8 g · kg−1 · d−1), and 5–9 mg · kg−1 · d−1 less DHA (22:6n–3) and arachidonic acid (20:4n–6) (estimated average requirement: 55–60 and 35–45 mg · kg−1 · d−1, respectively) than colostrum or transitional milk. Similar deficits were observed in measured intakes of a subset of OptiMoM infants. In multivariable-adjusted models, maternal ethnicity had medium to large [≥0.5 SD score (SDS)] effects on DHA, SFAs, and MUFAs. Mothers with prepregnancy BMI in overweight and obese categories had higher milk total fat (β: 0.35; 95% CI: 0.10, 0.61 and β: 0.46; 95% CI: 0.16, 0.77 SDS, respectively). Those with BMI ≥30 in addition had higher proportions of SFAs (β: 0.61; 95% CI: 0.33, 0.89 SDS) and lower DHA (β: −0.54; 95% CI: −0.89, −0.20 SDS). Other factors, such as gestational age and income, were also associated with FA composition. Conclusions The fat and FA content of human milk fed to VLBW infants is variable. Care must be taken to ensure fat and FA intakes meet recommendations, particularly when feeding a high proportion of donor milk. This trial was registered at clinicaltrials.gov as NCT02137473. [ABSTRACT FROM AUTHOR]

The number of online-purchased dietary supplements of diversified origin is exponentially increasing. Pomegranate seed oils (PSOs)—the main dietary source of conjugated linolenic acids (CLnA)—are available as dietary supplements via the Internet. PSO samples (n = 24) were purchased from Internet shops in Poland and China. Chromatographic techniques (gas chromatography coupled with mass spectroscopy [GC-MS] for fatty acids [FAs], liquid chromatography with fluorescence detection for tocopherols, and high-performance liquid chromatography with photodiode array detection for conjugated FAs) were used. The GC-MS method enabled the quantification of 45 FAs, which indicates its superiority to previously used methods. Argentometric liquid chromatography with pre-column mild alkaline hydrolysis for analysis of structural isomers of CLnA does not need preliminary derivatization into volatile compounds, which ensures the absence of artifacts and enables the analysis of the entire conjugated FA profile. PSO differed with tocopherols and FA profile. Some PSOs contained negligible amounts of CLnA, and because of that they did not meet the criteria of valuable, unadulterated nutraceuticals. Obtained results indicate that online purchased PSO shipped from distant places of origin could have been stored in inadequate conditions (temperature, humidity), which caused i.a. deterioration of oxidative quality. Unregulated online availability of dietary supplements of low oxidative quality and nutritive value as well as their intake may be a risk rather than a benefit for consumers. Argentometric liquid chromatography can be a simple, selective, and sensitive tool for screening of dietary supplements containing conjugated FAs and should be considered as an essential way of quality assurance. [ABSTRACT FROM AUTHOR]

The combination of solid phase microextraction (SPME) and gas chromatograph-mass spectrometer (GC–MS) is frequently used for comprehensive analysis of aroma components in foods because it can be used to easily analyze volatile components, allowing saving of the amount of solvent used. In this study, SPME-GC-MS analysis of sake samples before and after sherry cask storage was performed to investigate the special flavor derived from sherry cask storage. A GC column with polyethylene glycol as the stationary phase, which is the first choice for volatile component analysis, was used. However, the peak of the acid having a carbonyl group was tailed due to its bond with the hydroxyl group of the stationary phase. In the analysis of sake samples, a large and tailing peak derived from the large amount of fatty acids in Japanese sake was observed. Additionally, it was not possible to analyze other co-eluting components. To overcome this problem, a novel extraction condition was examined using SPME and tris (hydroxymethyl) aminomethane (Tris). By adding Tris solution to sake, the fatty acid peak was removed successfully, thereby facilitating analysis of the peaks of compounds co-eluting with fatty acids and comprehensive analysis of the aroma components in sake. Furthermore, a comparative analysis of sake before and after storage in sherry cask showed that levels of fatty alcohols, organic acid esters, fatty acid esters, and terpenes increased significantly after storage in sherry cask, suggesting that these ingredients might constitute the special flavor of sherry cask-stored sake. [ABSTRACT FROM AUTHOR]

The study of the influence of the season on the organoleptic and physicochemical parameters of milk is still relevant. In addition, the ratio of fatty acids in the milk of cows characterizes the level of their feeding and the study of these indicators is relevant given the prevalence of adulteration of milk and dairy products with vegetable oils. The aim was to study individual indicators of raw milk quality depending on the season and its fatty acid composition. The material for the study was prefabricated chilled raw milk obtained at different times of the year on the dairy farm of NULES of Ukraine "Agronomic Research Station". The research was conducted in the accredited Ukrainian Laboratory of Quality and Safety of AIC NULES of Ukraine. Organoleptic, physicochemical, sanitary and hygienic were determined by generally accepted standard methods. The fatty acid composition of milk was investigated by gas chromatography. Extraction of lipids from milk was performed by the method of Folch. Analysis of LCD methyl esters was performed on a Trace GC Ultra gas chromatograph (USA) with a flame ionization detector. Identification of fatty acids was performed using a standard sample Supelco 37 Component FAME Mix. Quantitative evaluation of the fatty acid spectrum of milk fat lipids was performed by the method of internal normalization with their determination in percent. The study was performed in 2 parallels. Statistical processing of experimental data was performed by conventional methods of variation statistics. The probability of the difference in indicators was assessed by Student's t-test. Differences between the compared indicators were considered significant at the significance level P < 0.05. Studies have shown that milk organoleptic and physicochemical parameters of raw milk vary depending on the season. In terms of microbiological indicators, the milk corresponded to the first grade, although in winter, according to other indicators, it could be attributed to the extra, or at worst, to the highest grade. In addition, in winter the content of saturated fatty acids in milk increases. The content of unsaturated fatty acids increases in summer and autumn due to the presence of green mass and corn silage in the diet of cows. The obtained results can be used as a basis for preparing a diet to obtain milk with optimal content and ratio of fatty acids, which can become competitive in the market of raw milk to produce milk and dairy products of improved chemical composition, dietary, functional products, baby food. [ABSTRACT FROM AUTHOR]

• A rapid and practicable analytical method for analysis of short-chain fatty acids in human plasma was developed. • Separation and detection of acetic, propionic, and butyric acids, was carried out by using GC–MS technique. • No tedious procedures such as evaporation and redissolution affecting the recovery of volatile acids were included. • The signals of target compounds were acquired in SIM mode allowing the obtainment of highly sensitive and selective data. A rapid and practicable analytical method for the measurement of short-chain fatty acids (SCFAs) in human plasma was developed. The extraction procedure involved the use of acidified water and methyl tert -butyl ether (MTBE), while the separation and detection of SCFAs, including acetic, propionic, and butyric acids was carried out by using gas chromatography-mass spectrometry (GC–MS) technique. The novelty of the research involves reducing the analysis time (less than 7 min) by using the novel fast GC–MS method. A narrow-bore GC capillary column of dimensions 30 m × 0.25 mm ID × 0.25 μm d f with acid-modified poly(ethylene glycol) stationary phase was employed for the chromatographic separation. The signals of target compounds were acquired in selected ion monitoring (SIM) mode monitoring a quantifier ion (Q) and two qualifier ions (q1 and q2). Linearity of the method, limits of detection (LoD) and quantification (LoQ) were evaluated. In detail, regression coefficients of the calibration curves were between 0.9960 and 0.9933; LoDs ranged from 0.02 μM to 0.03 μM, while LoQs from 0.06 μM to 0.10 μM. [ABSTRACT FROM AUTHOR]

Biofilm communities on intertidal mudflats are recognized as major producers of nutrients, especially fatty acids. The rising threats posed by both climatic and anthropogenic stressors increase the necessity of understanding and conserving these communities. Shorebirds provide a proxy for studying the complex ecology of biofilm communities because of their heavy reliance on fatty acids from diatomaceous biofilm for successful long-distance migration. Herein, we review biofilm feeding patterns by migratory shorebirds, experimental design considerations for sampling and studying the fatty acid content of biofilm, and the literature describing established and emerging analytical methodology. Techniques for fatty acid analysis include the commonly employed gas chromatography–flame ionization detection (GC/FID) and gas chromatography–mass spectrometry (GC/MS) with derivatization. Liquid chromatography–mass spectrometry (LC/MS) and liquid chromatography–quadrupole time of flight (LC/QTOF) are newly emerging techniques that enable derivatization to be eliminated. In addition, Fourier transform infrared spectroscopy (FT/IR), a common instrument in chemistry laboratories, has applications in fatty acid research, specifically for screening. Using a combination of sampling and analytical methods is necessary for improved understanding of intertidal biofilm, both as a source of essential fatty acids in aquatic systems and a critical food for shorebirds. [ABSTRACT FROM AUTHOR]

Introduction: Recently, new commercial infant formulas have been composed considering novel fat blends and oligosaccharides to better resemble the fatty acid (FA) composition and stereospecific distribution (e.g., increased amount of ß-palmitate) as well as probiotics content of human breast milk. We hypothesized that these newly composed infant formulas may decrease fecal FA soap excretion and may positively affect erythrocyte FA profiles compared with regular formulas. Methods: Healthy infants were randomly assigned to receive a high-sn-2-palmitate formula (>25% of the PA is esterified to the sn-2 position of the glycerol backbone, verum: n = 30) or a "standard" formula containing <10% of PA in sn-2 position and no oligosaccharides (control: n = 27); a non-randomized group of breast-fed infants served as control. Anthropometric data of the infants (body weight, recumbent length, and head circumference) were recorded at inclusion (visit 1) and 6 and 12 weeks after onset of intervention (visits 2 and 3). Blood samples for erythrocyte FA analysis (gas chromatography) were taken at visits 1 and 2; stool samples were collected at visit 2. Results: Quantitative formula intake (mL/kg body weight × day) at visit 2 (verum: 155 ± 30, control: 164 ± 30) and visit 3 (verum: 134 ± 26, control: 134 ± 21) was comparable. Six weeks after onset of intervention, stool total FA soaps, palmitate soaps, and total FAs were similar in both formula-fed groups but significantly higher than in breast-fed infants. During the 6-week intervention, erythrocyte palmitate decreased significantly from baseline in all 3 groups with no group differences (verum: 29.20 ± 1.17 to 27.12 ± 0.66, control: 29.88 ± 2.00 to 27.01 ± 0.94, breast-fed: 30.20 ± 0.86 to 26.84 ± 0.98). For selected FAs, significant changes over time in verum and control group were obvious but without formula effects. Some variations in the FA profile of breast-fed infants compared to both verum and control groups were observed. Conclusions: In contrast to our hypothesis, feeding a newly composed infant formula based on a fat blend with 25% of PA in the sn-2 position of triacylglycerols and supplemented with a prebiotic could not decrease insoluble FA soap excretion compared with a standard product; in this respect, breastfeeding is obviously the best choice. Surprisingly, erythrocyte FA profiles were comparable in formula-fed and breast-fed infants; obvious alterations in FA composition of the respective fat sources and structure did not affect FA incorporation into membranes. Caution should be, however, exercised in drawing robust conclusions in the absence of larger, adequately powered intervention studies. [ABSTRACT FROM AUTHOR]

Background and Objectives: Breast milk composition is affected by several factors such as maternal diet and body mass index. Milk fats include a major calorie content of the milk, which changes dramatically throughout the lactation. The aim of the present study was to assess breast milk fatty acid profile in overweight and obese mothers and its correlations with maternal body mass index. Materials and Methods: Milk samples were collected from 73 healthy lactating women referring to various health centers in Mashhad. Data were collected using food frequency questionnaire to assess the maternal dietary intakes. Breast milk fatty acid profile was assessed using gas chromatography. Results: Breast milk content of saturated fatty acids was higher in obese mothers than overweight mothers (p = 0.03) with positive correlations with maternal body mass index (r = 0.256; p = 0.037). Furthermore, breast milk monounsaturated fatty acids were negatively correlated with pre-pregnancy body mass index (r = -0.385; p = 0.003) and pre-pregnancy body weight (r = -0.276; p = 0.026). However, no significant correlations were observed between the maternal body fat mass and fat proportion with the breast milk fatty acids (p > 0.05). Moreover, no significant associations were seen between dietary saturated fatty acids, milk monounsaturated fatty acids, polyunsaturated fatty acids, n-3 polyunsaturated fatty acids and n-6 polyunsaturated fatty acids with the corresponding fatty acids in breast milk (p > 0.05). Conclusions: Based on the results, breast milk of the obese mothers included higher proportion of saturated fatty acids, while no significant differences were observed in the contents of milk monounsaturated fatty acids and polyunsaturated fatty acids between the overweight and obese women. Furthermore, maternal pre-pregnancy body mass index was negatively correlated with the contents of milk monounsaturated fatty acids, while it was positively correlated with saturated fatty acids contents. [ABSTRACT FROM AUTHOR]

Crustaceans are low in fat but rich in omega-3 (n-3) fatty acids (FA). In this study, a mini-scale extraction method was optimized based on the Bligh and Dyer method for the determination of n-3 fatty acids, i.e., alpha-linolenic acid (LNA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), in shrimp samples followed by gas chromatography with flame ionization detection. The optimal extraction conditions were determined through a 23 factorial design with a center point. The optimized method extracted comparable amounts of n-3 FA as the original Bligh and Dyer method but was 45 times faster and used 30 times less chloroform and sample and 11 times less total solvent volume. Additionally, the optimized method was successfully applied for the extraction of fatty acid, mainly n-3 fatty acid, from various shrimp species. Thus, the n-3 mini-scale extraction method can be considered a suitable alternative to traditional methods for the extraction of n-3 fatty acid from shrimp. [ABSTRACT FROM AUTHOR]

In this study, the effects of different concentrations of chitosan (C)/basil seed gum (B) and nettle essential oil (N) on the quality and shelf life of beef burger were examined during storage at refrigerator (4 ± 1 °C). At first, the chemical compounds of N were measured using the gas chromatography. Then, different concentrations of C (1, 1.5 and 2% w/w), B (0.5, 1 and 1.5% w/w) and N (0.5, 1 and 1.5% w/w) were added in the form of combination to the beef burger. 14 produced treatments (control + 13 treatments) were analyzed by microbial parameters (total viable count, psychrotrophic counts) and chemical parameters (peroxide value, the thiobarbituric acid, the total volatile basic nitrogen and free fatty acid) evaluation at 3-day intervals within 12 days. According to the results, the major constituents of essential oil were phytol (27.34%), α-limonene (19.73%), γ-terpinene (17.21%), and p-cymene (16.41%). The use of C + B + N led to decrease in oxidation and microbial spoilage. The minimum microbial and chemical quality loss during the storage period was observed in treatments (T13: C 2%+ N 1.5% + B 1%), (T8: C 1.5% + N 1.5% + B 1.5%) and (T11: C1% + N 1.5% + B 1%) respectively, and these three treatments had acceptable level of chemical and microbiological parameters by the end of the storage period; however, only T11 was approved by the sensory panelist. According to the results, T11 can be applied in the meat-product industry in order to improve the quality of beef burger. [ABSTRACT FROM AUTHOR]

Different Oil/Water (W/O) emulsions with pumpkin oil were prepared. This oil is considered as a source of essential fatty acids and biologically active nutrients that are used in foods. The oil was stabilified with soybean protein isolate (SI). Infrared analysis was performed to investigate the impact of pumpkin oil in the continuous phase and dispersed phase content in the emulsions. Droplet size distribution was analysed to obtain conditions for emulsion preparations. And at the end the different oils were investigated using gas chromatography (GC) analysis to determine fatty acid compositions. [ABSTRACT FROM AUTHOR]

Introduction: Although lipid is the major energy source and exerts beneficial effects on infant growth, research on the composition of fatty acid (FA) at the sn-2 position of human milk (HM) in China and abroad is limited. Objectives: This study aimed to investigate the FA positional distribution in colostrum and mature HM of women living in the inland and coastal areas of China and explore the potential influences of geographical region and lactation stage on the FA profile of Chinese women. Methods: Colostrum milk (n = 61) and mature milk (n = 56) samples were obtained longitudinally from healthy lactating women in Guangzhou and Chengdu, China. Gas chromatography was used to determine the total and sn-2 FA composition. Results: Significant differences were observed in the FA profile of HM between different regions and lactation stages, with differences in polyunsaturated FA levels being the most pronounced. Nearly 70% of sn-2 FAs were saturated FAs, of which C16:0 accounted for approximately 75%. C8:0, C10:0, C18:0, C20:0, C22:0, and all of the unsaturated FAs were mainly located at the sn-1 and sn-3 positions, while C14:0, C15:0, and C16:0 were mainly at the sn-2 position. The proportion of C12:0 and C17:0 at sn-2 was approximately equivalent to that at the sn-1, 3 positions. Conclusions: The results indicate the variability in the FA profile of HM between regions and lactation stages. The contents of polyunsaturated FAs and sn-2 FAs, especially palmitic acid, should be paid more attention when optimizing infant formula. [ABSTRACT FROM AUTHOR]

Historically considered to be simple membrane components serving as structural elements and energy storing entities, fatty acids are now increasingly recognized as potent signaling molecules involved in many metabolic processes. Quantitative determination of fatty acids and exploration of fatty acid profiles have become common place in lipid analysis. We present here a reliable and sensitive method for comprehensive analysis of free fatty acids and fatty acid composition of complex lipids in biological material. The separation and quantitation of fatty acids are achieved by capillary gas chromatography. The analytical method uses pentafluorobenzyl bromide derivatization and negative chemical ionization gas chromatography-mass spectrometry. The chromatographic procedure provides base line separation between saturated and unsaturated fatty acids of different chain lengths as well as between most positional isomers. Fatty acids are extracted in the presence of isotope-labeled internal standards for high quantitation accuracy. Mass spectrometer conditions are optimized for broad detection capacity and sensitivity capable of measuring trace amounts of fatty acids in complex biological samples. .

Comparative studies on esterification methods for the quantitative determination of fatty acids (FAs) were accomplished using a rotatable central composite design (RCCD). The effect of the three methylation methods on the isomerization of unsaturated fatty acids in gas chromatography analysis was also evaluated. The experimental design showed that the effect of both temperature and duration time of reaction on the recovery of methylation was significant. After optimization, the method was validated and the results for linearity, precision, and limit of detection (LOD) were presented. Methods were similar on account of the fatty acid methyl esters recovery (%) determined (91.30–96.44%). The acid-catalyzed procedures gave no isomerization of conjugated dienes and no artifacts, but artifacts were observed by using a methyl chloroformate reagent. Therefore, the optimized acid-catalyzed methylation process would be more convenient for polyunsaturated fatty acid analysis, whereas methyl chloroformate reagent would be an alternative for fast analysis. [ABSTRACT FROM AUTHOR]

When multiple metabolic pathways lead to the same product, compound-specific isotope analysis may not provide enough information to quantify the activities of the contributing pathways. Instead, identification of where in the molecule the 13C is incorporated is required. Here we show how knowledge of position-specific 13C incorporation in fatty acids (FA) and FA fragments can be used to quantitatively estimate the fluxes through the central C metabolic network. We developed a method to measure 13C enrichment of FA and FA fragments (ethanoate, propionate) using electron impact GC–MS. We tested the accuracy and repeatability of the measurements using natural abundance and position-specific 13C labelled standards and FA extracted from Bacillus licheniformis and Pseudomonas fluorescens grown with labelled and unlabelled glucose. The molecular ions of FA generally reflected theoretical predictions of mass isotopomer distributions for natural abundance values, but that of the associated FA fragments deviated from expected values, likely associated with McLafferty rearrangements of hydrogen. After correction for naturally occurring isotopes, 13C enrichments of FA and FA fragments showed good agreement with expected isotope composition of FA standards (root mean square error < 0.044 at%; δ13C of ∼ 40‰), natural abundance and labelled glucose. The unsaturated FA extracted from P. fluorescens deviated from expected values likely associated with problems of co-elution and ion suppression and were excluded from analysis. The ratio of glucose-1-13C to glucose-3-13C incorporation into FA fragments was high for B. licheniformis, but low for P. fluorescens. Metabolic flux modelling based on the 13C enrichment of ethanoate and propionate fragments showed that B. licheniformis used Embden-Meyerhof-Parnas and pentose phosphate pathway (66% and 30%, respectively), whereas P. fluorescens utilized Entner-Doudoroff and pentose phosphate pathway (72% and 27%, respectively). FA fragment analysis is therefore a promising tool to study central C metabolic network activities of co-occurring groups of microbes in intact and complex environmental communities. [ABSTRACT FROM AUTHOR]

In this research work, the characterization of the palm stearin biodiesel was made using Nuclear Magnetic resonance (NMR), Fourier transform infrared spectroscopy (FITR) and GC/MS methods. Analysis of the composition of fatty acids was done using the GCMS apparatus based on the retention time. Fourier transform infrared spectrometer was used for the spectrum analysis of the various functional groups and bands located in it. The properties of the palm stearin biodiesel were predicted adopting the American Society for Testing and Materials (ASTM) standards. Measured values of the properties were the density at 18°C as 0.88 g/m³, kinematic viscosity at 35°C as 3.4 mm² /s, the calorific value of the palm stearin as 37121 kJ/kg and the flash and fire points of the biodiesel as 130°C and 160°C respectively. The rapid and correct characterization of the palm stearin biodiesel was made by the NMR. [ABSTRACT FROM AUTHOR]

In this study, fatty acid analysis and lipid nutrition assessment of five common cod products from fishery markets are performed by gas chromatography (GC). Meanwhile, a lipid‐based rapid identification method for cod products using thin‐layer chromatography (TLC) is established. GC analysis showed that 13, 11, 13, 13, and 6 types of fatty acids are identified in Atlantic cod, Pacific cod, sablefish, silver cod, and oilfish, respectively. The nutrition assessment shows that Atlantic cod, Pacific cod, sablefish, and silver cod have healthier atherogenicity index (AI) and thrombogenic index (TI), suggesting these four types of fish have higher nutritive value. In terms of the fatty acid, the five species of fish can be classified by systematic clustering and heat map analysis. The TLC‐rapid lipid identification method shows that sablefish and silver cod contain characteristic triglyceride, and oilfish contains characteristic wax ester, which are obviously different from the Atlantic cod and the Pacific cod. The oilfish could be distinguished from the high nutritive value of sablefish, silver cod, Atlantic cod, and Pacific cod by the rapid identification method in a few minutes. This study provides important nutritional information and a method for rapid identification of cod products. [ABSTRACT FROM AUTHOR]