1. Transcriptome analysis to identify candidate genes associated with the yellow-leaf phenotype of a Cymbidium mutant generated by γ-irradiation.
- Author
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Kim SH, Kim SW, Lim GH, Lyu JI, Choi HI, Jo YD, Kang SY, Kang BC, and Kim JB
- Subjects
- Carotenoids metabolism, Chlorophyll metabolism, Chromosome Mapping, Gene Expression Regulation, Plant, Gene Ontology, Ion Transport genetics, Molecular Sequence Annotation, Phenotype, Pigmentation genetics, Gamma Rays, Gene Expression Profiling, Genetic Association Studies, Mutation genetics, Orchidaceae genetics, Orchidaceae radiation effects, Plant Leaves genetics
- Abstract
Leaf color is an important agronomic trait in flowering plants, including orchids. However, factors underlying leaf phenotypes in plants remain largely unclear. A mutant displaying yellow leaves was obtained by the γ-ray-based mutagenesis of a Cymbidium orchid and characterized using RNA sequencing. A total of 144,918 unigenes obtained from over 25 million reads were assigned to 22 metabolic pathways in the Kyoto Encyclopedia of Genes and Genomes database. In addition, gene ontology was used to classify the predicted functions of transcripts into 73 functional groups. The RNA sequencing analysis identified 2,267 differentially expressed genes between wild-type and mutant Cymbidium sp. Genes involved in the chlorophyll biosynthesis and degradation, as well as ion transport, were identified and assayed for their expression levels in wild-type and mutant plants using quantitative real-time profiling. No critical expression changes were detected in genes involved in chlorophyll biosynthesis. In contrast, seven genes involved in ion transport, including two metal ion transporters, were down-regulated, and chlorophyllase 2, associated with chlorophyll degradation, was up-regulated. Together, these results suggest that alterations in chlorophyll metabolism and/or ion transport might contribute to leaf color in Cymbidium orchids., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2020
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